##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936491_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1011434 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.574938157111585 31.0 31.0 34.0 30.0 34.0 2 31.597259929960828 31.0 31.0 34.0 30.0 34.0 3 31.57615919575573 33.0 31.0 34.0 28.0 34.0 4 35.25711316803667 37.0 35.0 37.0 33.0 37.0 5 35.291560299535114 37.0 35.0 37.0 33.0 37.0 6 35.33217095727452 37.0 35.0 37.0 33.0 37.0 7 35.255114026224156 37.0 35.0 37.0 32.0 37.0 8 35.21559884283107 37.0 35.0 37.0 32.0 37.0 9 36.71944882216734 39.0 37.0 39.0 32.0 39.0 10-11 36.57904766895319 39.0 37.0 39.0 32.0 39.0 12-13 36.492411269544036 39.0 37.0 39.0 32.0 39.0 14-15 37.50419206789568 40.0 37.0 41.0 32.0 41.0 16-17 37.451853012653324 39.5 37.0 41.0 32.0 41.0 18-19 37.54070161770318 40.0 37.0 41.0 32.0 41.0 20-21 37.467879762792236 40.0 37.0 41.0 32.0 41.0 22-23 37.51927758014858 40.0 37.0 41.0 32.0 41.0 24-25 37.54776535097693 40.0 37.0 41.0 32.0 41.0 26-27 37.43681396907756 40.0 37.0 41.0 32.0 41.0 28-29 37.29418231936043 39.5 36.0 41.0 31.5 41.0 30-31 36.985005447710876 39.0 36.0 41.0 31.0 41.0 32-33 36.69139162812403 39.0 35.0 41.0 31.0 41.0 34-35 36.45557940508229 39.0 35.0 40.5 30.0 41.0 36-37 36.202402232869375 38.0 35.0 40.0 30.0 41.0 38-39 35.96576000015819 38.0 35.0 40.0 30.0 41.0 40-41 35.699132123302164 38.0 35.0 40.0 29.0 41.0 42-43 35.34412576599165 38.0 35.0 40.0 27.5 41.0 44-45 34.88310112177364 38.0 34.5 40.0 25.5 41.0 46-47 34.34653966546507 38.0 34.0 40.0 23.0 41.0 48-49 33.98721023813714 38.0 33.0 40.0 20.5 41.0 50-51 32.92603966249899 37.0 32.0 39.5 16.5 40.5 52-53 32.990067567433954 37.5 33.0 39.5 15.0 40.5 54-55 33.394816171890604 38.0 33.0 40.0 14.5 41.0 56-57 33.28433244284847 38.0 33.0 40.0 13.5 41.0 58-59 33.061150801732985 37.0 33.0 40.0 10.5 41.0 60-61 32.750325280740014 37.0 33.0 40.0 8.0 41.0 62-63 32.383038833972364 36.0 32.0 39.5 7.0 41.0 64-65 32.02592803880431 36.0 31.5 39.0 4.0 41.0 66-67 31.577125151023203 35.0 31.0 39.0 2.0 40.5 68-69 31.208864839426003 35.0 31.0 38.5 2.0 40.0 70-71 30.818902666906588 35.0 30.5 37.5 2.0 40.0 72-73 30.435966657241103 35.0 30.0 37.0 2.0 39.5 74-75 30.03194919292806 35.0 30.0 36.5 2.0 39.0 76-77 29.596538676769814 34.5 29.0 36.0 2.0 39.0 78-79 29.125128777557407 34.0 29.0 35.5 2.0 37.5 80-81 28.682039065327054 34.0 29.0 35.0 2.0 37.0 82-83 28.27974736858757 34.0 27.5 35.0 2.0 37.0 84-85 27.93283891979111 34.0 27.0 35.0 2.0 36.0 86-87 27.592870617361093 34.0 26.5 35.0 2.0 36.0 88-89 27.1834751451899 34.0 26.0 35.0 2.0 35.5 90-91 26.760596341432063 33.0 25.0 35.0 2.0 35.0 92-93 26.314084754912333 33.0 24.5 35.0 2.0 35.0 94-95 25.845396733746345 33.0 23.0 35.0 2.0 35.0 96-97 25.281077163710137 33.0 19.0 35.0 2.0 35.0 98-99 24.583229849896284 32.0 5.0 35.0 2.0 35.0 100-101 21.8945724585094 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41981464458149276 1101 2 0.4383312138526705 1101 3 0.5487440067153706 1101 4 0.5609773426107338 1101 5 0.46474178772793096 1101 6 0.6388285458609886 1101 7 0.5217390063838323 1101 8 0.4907050279553715 1101 9 0.6255794479476862 1101 10-11 0.7800631724757494 1101 12-13 0.663675360874386 1101 14-15 0.6441430242275885 1101 16-17 0.8879453529696519 1101 18-19 0.6695303327492041 1101 20-21 0.7635018813409502 1101 22-23 0.8650306214315222 1101 24-25 0.7221369656097423 1101 26-27 0.6328948358489868 1101 28-29 0.7001299865192863 1101 30-31 0.74044763814004 1101 32-33 0.7738600501151822 1101 34-35 0.6106763103300139 1101 36-37 0.6014646878389485 1101 38-39 0.31411670850234685 1101 40-41 0.4712361110275296 1101 42-43 0.7227254322647809 1101 44-45 0.7568183246206033 1101 46-47 0.9463388001684407 1101 48-49 0.9253424254772611 1101 50-51 0.7678084326620009 1101 52-53 0.7498123644788137 1101 54-55 0.8373195503893172 1101 56-57 0.8634351494800825 1101 58-59 0.6980595954426931 1101 60-61 0.7951678521285643 1101 62-63 0.9248863129932445 1101 64-65 0.6655519481639693 1101 66-67 0.93307835824017 1101 68-69 0.7727660798439793 1101 70-71 0.6758722069188714 1101 72-73 0.6906158535270954 1101 74-75 0.7634853958543886 1101 76-77 0.6597606947792372 1101 78-79 0.788922795550075 1101 80-81 0.8254781042720829 1101 82-83 0.7316490587497242 1101 84-85 0.6958562686923528 1101 86-87 0.7697455979617409 1101 88-89 0.4908362517987328 1101 90-91 0.36889959441667486 1101 92-93 0.38561148982927307 1101 94-95 0.5063103754038458 1101 96-97 0.22609995643872693 1101 98-99 0.3664984691851423 1101 100-101 0.4261853578065775 1102 1 0.3734408619203897 1102 2 0.28606520741801233 1102 3 0.40119667900730605 1102 4 0.38820302401699536 1102 5 0.38383590547701374 1102 6 0.4018919038518547 1102 7 0.42335244112233283 1102 8 0.38493204485719446 1102 9 0.6204900391212789 1102 10-11 0.5946970385619679 1102 12-13 0.48495743661606383 1102 14-15 0.5219912503599389 1102 16-17 0.40805204085393143 1102 18-19 0.2640648821928906 1102 20-21 0.277930003061833 1102 22-23 0.1850498300203114 1102 24-25 0.21378524030556179 1102 26-27 0.07058670493573516 1102 28-29 0.1522472673115658 1102 30-31 0.45063588664628185 1102 32-33 0.20798391299273788 1102 34-35 0.4215497591172337 1102 36-37 0.17645731659718678 1102 38-39 0.1317597756326805 1102 40-41 0.11475093589549346 1102 42-43 -0.010151606750767428 1102 44-45 0.29390759469422534 1102 46-47 0.3519749816917752 1102 48-49 0.31646810081308985 1102 50-51 0.38535914246921976 1102 52-53 0.4308679479776458 1102 54-55 0.3076963726036368 1102 56-57 0.33146455639864314 1102 58-59 0.3525364323994964 1102 60-61 0.4355461052309906 1102 62-63 0.606906119953436 1102 64-65 0.5768444737248366 1102 66-67 0.452885318426798 1102 68-69 0.3000114901619817 1102 70-71 0.15674817060033064 1102 72-73 0.02744637133514871 1102 74-75 0.15660672649360663 1102 76-77 0.23999937125110193 1102 78-79 0.26092345896183033 1102 80-81 0.380644802736267 1102 82-83 0.38310585460101976 1102 84-85 0.2122437315109451 1102 86-87 0.3586725836441893 1102 88-89 0.5682353741873634 1102 90-91 0.5221872766381068 1102 92-93 0.3973301382036958 1102 94-95 0.391382960454969 1102 96-97 0.08149570540024698 1102 98-99 0.22948713915296892 1102 100-101 0.4247598897327265 1103 1 0.22897073537599866 1103 2 0.2813135570197538 1103 3 0.36163077116354714 1103 4 0.4075858498978988 1103 5 0.42356186453716305 1103 6 0.5078063159442436 1103 7 0.5491859545998921 1103 8 0.539603877505975 1103 9 0.3182696922466448 1103 10-11 0.5471784559921673 1103 12-13 0.19590493981709045 1103 14-15 0.5590015352095392 1103 16-17 0.7654432891702712 1103 18-19 0.806176020094675 1103 20-21 0.6567026388767161 1103 22-23 0.36841240214729254 1103 24-25 0.4488329230965249 1103 26-27 0.35815405116184706 1103 28-29 0.5109033359548221 1103 30-31 0.3794785708506794 1103 32-33 0.5153092700940292 1103 34-35 0.5678490424203559 1103 36-37 0.6481989232619725 1103 38-39 0.46704213092143476 1103 40-41 0.48312855699398227 1103 42-43 0.8870497834083722 1103 44-45 0.9013185895892164 1103 46-47 0.9726224200408708 1103 48-49 0.9827197428969967 1103 50-51 1.1402666869094205 1103 52-53 1.2143820491133823 1103 54-55 1.311619705992399 1103 56-57 1.1655579597942207 1103 58-59 1.0246777513878058 1103 60-61 1.039958738052718 1103 62-63 1.259125219675731 1103 64-65 1.1097422737481146 1103 66-67 1.1035745779508837 1103 68-69 1.1856309922987727 1103 70-71 0.9926062945506402 1103 72-73 0.7676198502250173 1103 74-75 0.9024994381353579 1103 76-77 0.9991981590190306 1103 78-79 0.8228102757302089 1103 80-81 0.9840264562939751 1103 82-83 0.7737025338500629 1103 84-85 0.906646719687938 1103 86-87 0.9076285092806486 1103 88-89 0.6360284965579481 1103 90-91 0.7234724671198407 1103 92-93 0.5849132816927316 1103 94-95 0.9242017741636666 1103 96-97 0.8471225241640674 1103 98-99 1.1251701213436327 1103 100-101 0.9537457885969864 1104 1 0.39092088879406006 1104 2 0.4796790684034349 1104 3 0.4074344866986799 1104 4 0.5310918220678431 1104 5 0.5163546644315318 1104 6 0.5652981819295775 1104 7 0.5022027823046074 1104 8 0.5023667426498264 1104 9 0.5028236368742469 1104 10-11 0.8188786867597315 1104 12-13 0.7502944502390747 1104 14-15 0.5631754523657477 1104 16-17 0.5315955405827566 1104 18-19 0.3682990028491915 1104 20-21 0.3888506690755449 1104 22-23 0.3939855274810782 1104 24-25 0.34503974285937744 1104 26-27 0.21716709120426003 1104 28-29 0.32665090658920803 1104 30-31 0.4615335845765216 1104 32-33 0.5307826194950351 1104 34-35 0.4866759115686463 1104 36-37 0.3889485138515454 1104 38-39 0.3697182266732213 1104 40-41 0.0756252610183239 1104 42-43 0.3021313600375848 1104 44-45 0.4069237188959036 1104 46-47 0.42411196455575606 1104 48-49 0.6614153248115926 1104 50-51 0.7829346437198197 1104 52-53 0.7425190395181005 1104 54-55 0.6373576793762936 1104 56-57 0.5562897156575133 1104 58-59 0.49239738949066236 1104 60-61 0.1288771653648766 1104 62-63 0.3117470229734778 1104 64-65 0.40380981316680575 1104 66-67 0.43678540018103007 1104 68-69 0.5146467124768428 1104 70-71 0.41995835993018815 1104 72-73 0.2791912374119967 1104 74-75 0.3570745873657053 1104 76-77 0.2737751015754455 1104 78-79 0.02212368803558107 1104 80-81 0.3248116178017071 1104 82-83 0.14741739894661876 1104 84-85 0.03236873049912958 1104 86-87 0.03390034204018377 1104 88-89 -0.07225745815170015 1104 90-91 -0.13116515291968867 1104 92-93 -0.1827288098378439 1104 94-95 -0.042921238151162555 1104 96-97 0.20076987349019149 1104 98-99 0.44680315165084394 1104 100-101 0.35362055137542114 1105 1 0.31806896109394245 1105 2 0.36616525091518426 1105 3 0.43754944107777405 1105 4 0.3331247291061672 1105 5 0.2729616123060765 1105 6 0.351707040867943 1105 7 0.4108035068030631 1105 8 0.4373896351138242 1105 9 0.678961113501444 1105 10-11 0.5688053465219483 1105 12-13 0.31653638398448436 1105 14-15 0.3950936862016121 1105 16-17 0.36795308478607325 1105 18-19 0.3907340861946338 1105 20-21 0.3504180413393456 1105 22-23 0.12810115668408173 1105 24-25 0.10916910285450143 1105 26-27 0.08020501724543294 1105 28-29 0.2311511385599374 1105 30-31 0.03936468177459318 1105 32-33 -0.1117964262852098 1105 34-35 0.12261543984798351 1105 36-37 0.2086126015036669 1105 38-39 0.02879109359700749 1105 40-41 -0.2577044795025074 1105 42-43 -0.11708940579383409 1105 44-45 0.08189150074033336 1105 46-47 0.46629642579789987 1105 48-49 0.44573082373609907 1105 50-51 0.20924996456578526 1105 52-53 0.33744145819947846 1105 54-55 0.427232040285233 1105 56-57 0.3131848695782793 1105 58-59 0.2388946316165459 1105 60-61 0.2815171796111642 1105 62-63 0.36564905166287076 1105 64-65 0.32102672687837597 1105 66-67 0.38672611877477436 1105 68-69 0.2635391108666383 1105 70-71 0.24763551292307895 1105 72-73 0.12900683240517807 1105 74-75 0.2799647082247958 1105 76-77 0.18854765311277788 1105 78-79 0.1627340287747927 1105 80-81 0.30582422857358793 1105 82-83 0.1863627054057133 1105 84-85 0.37428701119776164 1105 86-87 0.15618780765462859 1105 88-89 0.014048771316549136 1105 90-91 0.16425447976990526 1105 92-93 0.0040558885299262215 1105 94-95 0.22395146850194436 1105 96-97 0.1909563404589676 1105 98-99 0.36122376377236876 1105 100-101 0.6024567144391213 1106 1 0.26565752276701105 1106 2 0.34824850001103513 1106 3 0.48523908772961377 1106 4 0.5126831000655727 1106 5 0.34126810196170965 1106 6 0.6076721777819358 1106 7 0.6316809244188022 1106 8 0.594399316808456 1106 9 0.6849363589133048 1106 10-11 0.5778625606199341 1106 12-13 0.697078648737282 1106 14-15 0.8129250764570202 1106 16-17 0.7690650195525706 1106 18-19 0.6682819892429066 1106 20-21 0.3720614584608981 1106 22-23 0.38407303259665326 1106 24-25 0.30993285602744436 1106 26-27 0.30677913499820164 1106 28-29 0.559863765196944 1106 30-31 0.6858165990196952 1106 32-33 0.7184279823676647 1106 34-35 0.6704982711259149 1106 36-37 0.4578904054886621 1106 38-39 0.517377476327205 1106 40-41 0.4608951565915689 1106 42-43 0.605661453428489 1106 44-45 0.6230825064235503 1106 46-47 0.8046143717511356 1106 48-49 0.8608216879003479 1106 50-51 0.5396630651790346 1106 52-53 0.7151533435466462 1106 54-55 0.941599585268051 1106 56-57 0.9800784158639715 1106 58-59 1.081049314097399 1106 60-61 1.0354651096154228 1106 62-63 0.9979917522042427 1106 64-65 1.1423713817293368 1106 66-67 1.1779541889502134 1106 68-69 1.3464961834456481 1106 70-71 1.3919691382796842 1106 72-73 0.9964260205804827 1106 74-75 1.318361275828181 1106 76-77 1.3110693863832168 1106 78-79 1.2186519926986854 1106 80-81 1.4315784348318665 1106 82-83 1.245567054306136 1106 84-85 1.1674783762942411 1106 86-87 1.1869041632041935 1106 88-89 1.1908239358604291 1106 90-91 1.1831304148059587 1106 92-93 1.0667375607001084 1106 94-95 1.2436518076982068 1106 96-97 1.1497046837884817 1106 98-99 1.2354987598412883 1106 100-101 0.8731622875909473 1107 1 0.25696667791254413 1107 2 0.11495015119381335 1107 3 0.1878326929055909 1107 4 0.23770825868363943 1107 5 0.3165379362387597 1107 6 0.3506638290078996 1107 7 0.42385994148347095 1107 8 0.5148065192997038 1107 9 0.5584124809012891 1107 10-11 0.526078998159619 1107 12-13 0.3183604641313238 1107 14-15 0.12111015750448217 1107 16-17 0.19245799932633645 1107 18-19 0.2728278167994702 1107 20-21 0.21054269855401486 1107 22-23 0.37872774406077525 1107 24-25 0.34266324634234735 1107 26-27 0.39486280413969865 1107 28-29 0.35683263761576 1107 30-31 0.2711399466491713 1107 32-33 0.05586400605348274 1107 34-35 0.25958871689775975 1107 36-37 0.21979917796405601 1107 38-39 0.11225332448576353 1107 40-41 0.17909228786125198 1107 42-43 0.039380048346664864 1107 44-45 0.1107541357086177 1107 46-47 0.27310239279366755 1107 48-49 0.02737821812740293 1107 50-51 -0.18774276816509428 1107 52-53 0.01209889295471811 1107 54-55 0.12496250427241762 1107 56-57 -0.04076414949444995 1107 58-59 -0.07924801109879098 1107 60-61 0.0826713152639158 1107 62-63 0.003583228075711986 1107 64-65 -0.11058567369736849 1107 66-67 0.11619399269139663 1107 68-69 -0.13541811744709875 1107 70-71 -0.08782708771165204 1107 72-73 0.028761215435601173 1107 74-75 0.24521210789818326 1107 76-77 3.874474635878755E-4 1107 78-79 -0.10855343551611085 1107 80-81 -0.03966861199987548 1107 82-83 -0.0839127993635671 1107 84-85 -0.38245403016536983 1107 86-87 -0.24767867883423733 1107 88-89 -0.354665437052919 1107 90-91 -0.35003620219133325 1107 92-93 -0.19190771790189487 1107 94-95 -0.28804502771476237 1107 96-97 -0.11461802029866064 1107 98-99 -0.013232511565131944 1107 100-101 -0.43228752258565706 1108 1 0.11021997947446849 1108 2 0.1297667534295428 1108 3 0.11008323457791391 1108 4 0.23914350182036515 1108 5 0.25946251433760636 1108 6 0.3129981225214067 1108 7 0.2843657034023579 1108 8 0.08686192487947153 1108 9 0.09404639252115032 1108 10-11 0.19336064933437314 1108 12-13 0.1173883026534881 1108 14-15 0.305635253731964 1108 16-17 0.24524193532772642 1108 18-19 0.3818174827137497 1108 20-21 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-2.5262644029537 2316 58-59 -2.347490975257781 2316 60-61 -2.5676943231717715 2316 62-63 -2.7233266343052946 2316 64-65 -2.485737749246997 2316 66-67 -2.439562817852508 2316 68-69 -2.513894733484502 2316 70-71 -2.324251998062177 2316 72-73 -2.3166356829741765 2316 74-75 -2.3152139852621687 2316 76-77 -2.2478843359507863 2316 78-79 -3.0667772494206957 2316 80-81 -3.062560206540173 2316 82-83 -3.086005715646916 2316 84-85 -2.7760278994554923 2316 86-87 -2.584696871575918 2316 88-89 -2.7359390228441782 2316 90-91 -2.8918264693561575 2316 92-93 -3.408655272489508 2316 94-95 -3.19231159174608 2316 96-97 -3.4638576425505043 2316 98-99 -4.504551062904714 2316 100-101 -5.528389882572888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1501.0 3 616.0 4 588.0 5 681.0 6 772.0 7 981.0 8 1297.0 9 1689.0 10 2354.0 11 3669.0 12 5223.0 13 6917.0 14 7842.0 15 10477.0 16 14145.0 17 19551.0 18 13924.0 19 7927.0 20 7227.0 21 7552.0 22 8203.0 23 9161.0 24 10312.0 25 11919.0 26 13819.0 27 16250.0 28 19273.0 29 23057.0 30 28123.0 31 34096.0 32 43454.0 33 54897.0 34 73488.0 35 101507.0 36 135239.0 37 167149.0 38 128168.0 39 18383.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.83700721063519 18.48798161545125 14.019009496837684 19.656001677075878 2 15.786418807010605 21.659802734037033 40.048055768422614 22.50572269052975 3 19.530908537152147 29.43999351313294 29.444641116057568 21.58445683365735 4 11.977912004018876 17.06163199594948 37.517454026193896 33.44300197383775 5 12.2802298061641 39.85983762447916 32.00937976269715 15.850552806659593 6 28.438468470339895 36.60277544495849 18.707199360593194 16.251556724108426 7 27.669937471078892 35.49613791957855 20.305584888646315 16.52833972069624 8 27.976838597063786 31.72301256471496 19.744057596930272 20.556091241290986 9 29.623955445529866 16.158512160794874 20.42366704019575 33.79386535347951 10-11 25.052757249392556 27.6940074589626 25.576249618570156 21.67698567307469 12-13 27.97135018484709 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20.83895860573988 38-39 21.074109703756946 26.12313420661576 32.23817099105922 20.564585098568074 40-41 20.768119610289713 26.402585281258588 32.16835175960879 20.66094334884291 42-43 20.593424749365596 26.67064471587682 31.204187135740302 21.531743399017287 44-45 20.88017804864303 26.602047243004563 30.9170177445524 21.600756963800006 46-47 21.456043439959107 27.046159900100747 30.167517121984066 21.33027953795608 48-49 21.56674213736986 27.400264971377158 29.31211255351334 21.720880337739636 50-51 21.488036385208623 27.788609847735813 29.161360490409333 21.561993276646234 52-53 21.650026300281986 28.245069191493737 28.373851587311005 21.73105292091327 54-55 21.05454664105132 27.77194961700579 28.865364212807737 22.308139529135147 56-57 21.833523823836494 28.096327554022842 27.932449694687882 22.137698927452778 58-59 22.119670542635657 27.411604176554345 28.27361177028951 22.195113510520486 60-61 21.774043245931512 27.886205428803496 28.917991519688275 21.421759805576716 62-63 21.912752971791342 28.016703133295827 28.85635839264722 21.21418550226561 64-65 21.54765341675334 28.33884369426197 28.74510098593276 21.36840190305192 66-67 21.742567893299054 28.279113462465546 28.112485939257592 21.865832704977812 68-69 21.64041692941133 28.36277309989243 28.52212619162432 21.474683779071924 70-71 21.88955603490703 28.186048168063422 28.261677102536314 21.662718694493236 72-73 21.70790352350528 28.235953172875394 28.2585489366394 21.797594366979926 74-75 21.23251294375955 29.291369343441087 27.9533282893468 21.522789423452558 76-77 21.223872183239624 28.658092143976816 28.2916227803409 21.82641289244265 78-79 21.163145460948847 28.612385094200004 28.376538837467898 21.847930607383255 80-81 21.195265762772582 29.288058474885297 27.846472255056266 21.67020350728586 82-83 21.341218216414518 28.739330454322204 27.802868690086207 22.116582639177075 84-85 21.008213889443777 28.700650900798447 27.799704118020518 22.491431091737258 86-87 21.2416707377615 28.85544218023445 27.589117419604943 22.313769662399103 88-89 21.206252039676023 28.454049189568725 27.554712764168947 22.7849860065863 90-91 21.05193918832036 28.262544634467208 27.949089506325482 22.736426670886953 92-93 21.285896400000397 28.650215252304577 27.863874304126334 22.20001404356869 94-95 20.787010164316023 28.315169937594124 28.14998373353453 22.74783616455532 96-97 20.754967005299523 28.32911237185072 28.21341088958618 22.702509733263582 98-99 20.86583619595943 28.553056888974627 27.67614115528912 22.904965759776825 100-101 20.880274513140893 28.36319165062108 28.003625346555026 22.752908489682998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 779.0 1 630.0 2 1076.5 3 1529.5 4 1190.0 5 1056.5 6 1553.5 7 2855.5 8 4283.5 9 4823.5 10 4162.5 11 3282.5 12 3054.0 13 3182.0 14 3331.5 15 3341.5 16 3266.5 17 3236.5 18 3314.5 19 3359.0 20 3524.0 21 3788.5 22 4140.0 23 4594.5 24 5007.0 25 5646.0 26 6624.5 27 7719.0 28 8641.0 29 10051.5 30 12218.0 31 14513.5 32 17555.5 33 20212.0 34 22259.5 35 23946.5 36 26520.0 37 29093.5 38 28728.0 39 27769.5 40 27385.5 41 27530.5 42 27473.5 43 28469.0 44 30025.5 45 30791.5 46 30786.0 47 33590.0 48 44057.5 49 44206.5 50 35170.5 51 30710.5 52 27452.5 53 26669.5 54 26491.5 55 25356.0 56 23552.5 57 21744.5 58 21007.5 59 20285.5 60 18775.5 61 17423.5 62 15303.0 63 11141.0 64 7456.5 65 5563.0 66 4085.0 67 3142.0 68 2319.0 69 1530.0 70 1168.0 71 990.5 72 908.5 73 929.0 74 761.0 75 612.5 76 444.0 77 243.5 78 152.0 79 87.0 80 53.0 81 37.0 82 27.0 83 19.5 84 12.0 85 6.0 86 4.5 87 3.0 88 2.5 89 3.5 90 3.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014830428876229195 2 0.010776778316726549 3 0.015819124134644473 4 0.02036712232335476 5 0.03173711779513048 6 0.0491381543432394 7 0.006129910602174734 8 0.026595902451371025 9 0.035296420725425486 10-11 0.04305767850398543 12-13 0.03242920447602118 14-15 0.029908031567062208 16-17 0.03139107445468513 18-19 0.02590381577048033 20-21 0.05675110783303705 22-23 0.014583255061625375 24-25 0.003064955301087367 26-27 0.0014336081247021556 28-29 9.886952584152796E-5 30-31 0.003262694352770423 32-33 0.0020762600426720876 34-35 0.0 36-37 1.4830428876229195E-4 38-39 9.886952584152796E-5 40-41 0.0015819124134644473 42-43 0.004992911054997162 44-45 0.002471738146038199 46-47 0.0014830428876229196 48-49 3.9547810336611184E-4 50-51 0.003361563878611951 52-53 0.0037570419819780627 54-55 0.006031041076333206 56-57 9.886952584152796E-4 58-59 0.007316344912273069 60-61 0.003213259589849659 62-63 6.920866808906958E-4 64-65 5.932171550491678E-4 66-67 0.019427861827860247 68-69 0.04661698143428044 70-71 0.0570971511734824 72-73 0.018043688466078855 74-75 0.0325280740018627 76-77 0.029561988226616865 78-79 0.059914932659965954 80-81 0.08705461750346538 82-83 0.05482315207912725 84-85 0.021751295685136153 86-87 0.023728686201966713 88-89 0.024124164305332825 90-91 0.04389806947363842 92-93 0.02916651012325075 94-95 0.014929298402070725 96-97 0.0208614699525624 98-99 0.05551523876001795 100-101 0.0763272739496596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1011434.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.66212022574096 #Duplication Level Percentage of deduplicated Percentage of total 1 76.5679311183775 36.49389938400362 2 12.661166931712925 12.069161209949545 3 4.263803099631378 6.096656878605533 4 1.972775381029883 3.761066295561129 5 1.0863749378760288 2.5889466449639578 6 0.6771130690650977 1.9363586702520683 7 0.4485821523985026 1.4966263535117361 8 0.32184458982870917 1.227183642753618 9 0.25017133185188833 1.0731326486182597 >10 1.5095858736750827 14.081544576436631 >50 0.14782301235836193 4.895761969558507 >100 0.08467464732038538 7.6800265925109565 >500 0.004599610441898024 1.5584063453967305 >1k 0.002927024826662379 2.544031843338994 >5k 4.1814640380891127E-4 1.3849179223942556 >10k+ 2.0907320190445563E-4 1.1122790221445302 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11162 1.1035816474431352 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8513 0.8416762734889276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5385 0.5324123966566281 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4064 0.4018057530199697 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2541 0.25122746516332256 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2254 0.22285191124680406 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2232 0.22067678167829044 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2124 0.2099988728874054 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1759 0.17391149595524769 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1657 0.16382680431941185 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1561 0.15433532983862516 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1452 0.14355855152189861 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1318 0.13031003505913386 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 0.12932133980071858 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1162 0.1148863890278555 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1089 0.10766891364142395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.886952584152797E-5 0.0 5 0.0 0.0 0.0 9.886952584152797E-5 0.0 6 0.0 0.0 0.0 9.886952584152797E-5 0.0 7 0.0 0.0 0.0 1.9773905168305595E-4 0.0 8 0.0 0.0 0.0 1.9773905168305595E-4 0.0 9 0.0 0.0 0.0 2.966085775245839E-4 0.0 10-11 0.0 0.0 0.0 2.966085775245839E-4 0.0 12-13 0.0 0.0 0.0 2.966085775245839E-4 0.0 14-15 0.0 0.0 0.0 2.966085775245839E-4 0.0 16-17 0.0 0.0 0.0 4.943476292076398E-4 0.0 18-19 0.0 0.0 0.0 6.426519179699318E-4 0.0 20-21 0.0 0.0 0.0 8.403909696529878E-4 0.0 22-23 0.0 0.0 0.0 0.0011864343100983356 0.0 24-25 0.0 0.0 0.0 0.0014336081247021556 0.0 26-27 0.0 0.0 0.0 0.002026825279751323 0.0 28-29 0.0 0.0 0.0 0.003361563878611951 0.0 30-31 0.0 0.0 0.0 0.004894041529155634 0.0 32-33 0.0 0.0 0.0 0.00706917109766925 0.0 34-35 0.0 0.0 0.0 0.010579039265043493 0.0 36-37 0.0 0.0 0.0 0.0141383421953385 0.0 38-39 0.0 0.0 0.0 0.01735160178518816 0.0 40-41 0.0 0.0 0.0 0.021751295685136153 0.0 42-43 0.0 0.0 0.0 0.028326119153597763 0.0 44-45 0.0 0.0 0.0 0.039893853677056536 0.0 46-47 0.0 0.0 0.0 0.0673795818610013 0.0 48-49 0.0 0.0 0.0 0.1228453858580985 0.0 50-51 0.0 0.0 0.0 0.1613056314104529 0.0 52-53 0.0 0.0 0.0 0.1972447040538483 0.0 54-55 0.0 0.0 0.0 0.24188429497129815 0.0 56-57 0.0 0.0 0.0 0.2812838010191471 0.0 58-59 0.0 0.0 0.0 0.3311140420432772 0.0 60-61 0.0 0.0 0.0 0.4137195308838738 0.0 62-63 0.0 0.0 0.0 0.4825327208695772 0.0 64-65 0.0 0.0 0.0 0.5579701690866631 0.0 66-67 0.0 0.0 0.0 0.6868465960210948 0.0 68-69 0.0 0.0 0.0 0.8382158400844741 0.0 70-71 0.0 0.0 0.0 0.966845093204302 0.0 72-73 0.0 0.0 0.0 1.1101564709115967 0.0 74-75 0.0 0.0 0.0 1.2286021628697474 0.0 76-77 0.0 0.0 0.0 1.3255931677202861 0.0 78-79 0.0 0.0 0.0 1.445472467803139 0.0 80-81 0.0 0.0 0.0 1.658041948362424 0.0 82-83 0.0 0.0 0.0 1.891176290296747 0.0 84-85 0.0 0.0 0.0 2.1413161906758127 0.0 86-87 0.0 0.0 0.0 2.5171192583994606 0.0 88-89 0.0 0.0 0.0 2.960845690376238 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1050 0.0 42.075306 2 TATCACG 90 5.6929457E-7 36.947853 2 ATCACGC 105 4.6487912E-8 36.19739 3 GTACCTG 1375 0.0 32.474113 1 GTACATG 11815 0.0 26.977453 1 ACCTGGG 1630 0.0 26.232006 3 GTATCAA 16990 0.0 26.085577 1 TACATGG 12030 0.0 26.022764 2 TCACGCA 130 9.9969875E-6 25.584343 4 ACATGGG 11755 0.0 25.219622 3 GTATCAC 210 1.2641976E-9 24.882013 1 ATGGGAT 3175 0.0 24.693422 5 GAGTACT 8580 0.0 24.30946 12-13 CATGGGG 2335 0.0 24.214731 4 GTAAGGT 360 0.0 23.75689 4 ATGGGAG 2830 0.0 23.506212 5 CATGGGT 780 0.0 23.14774 4 CATGGGA 9035 0.0 23.033798 4 AGTACTT 8845 0.0 22.82912 12-13 GGTATCA 12440 0.0 22.758207 1 >>END_MODULE