FastQCFastQC Report
Thu 26 May 2016
SRR936491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011434
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125511.2409114188370176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106951.0574095788751416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.7032589373107885No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44380.43878295568470116No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.33002647725902035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27640.2732753694259833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.27050702270242055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21760.21514008823116487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.19912322504483734No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17010.16817706345643907No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.1637279347935703No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15540.15364324315773448No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15530.15354437363189294No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14800.14632689824546138No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13290.13139759984339067No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11660.11528186713122163No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11140.11014065178746216No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11030.10905308700320535No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.10836100032231466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.10282430687518909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG756.9159196E-638.0044332
TACCTGG9700.031.3438592
GTACCCG700.00838430827.1473661
GTACCTG12400.025.2864531
ACAACGC1151.2818805E-424.7818223
GTATCAA181350.024.3892171
GTACATG109450.024.0469341
GGTATCA135300.023.525711
GAGTACT106800.023.41684712-13
TACATGG112100.023.3501782
ACATGGG109800.022.537993
AGTACTT109300.022.35972612-13
ATATAGG4500.022.1692522
TAGTCGA1100.002878485521.5891555
GTACTTT117300.021.2407714-15
GTCGGGA6600.020.8736462
ATGGGAT28700.020.5209835
ATGGGAG26650.020.4955225
AGGTAAG2551.189801E-820.4925842
CATGGGA86600.020.40254
Produced by FastQC (version 0.11.5)