FastQCFastQC Report
Thu 26 May 2016
SRR936490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017957
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111881.0990641058512294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85910.8439452747021731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53820.5287060258930387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39900.3919615465093319No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24310.23881165903864307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23220.22810393759264883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.2198521155608734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.20708143860693526No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17410.1710288352062022No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17240.1693588236045334No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15240.14971162829078244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.14087039039959448No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13740.1349762318054692No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13390.13153797262556277No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11660.11454314867916818No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.10688074250680529No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10390.10206717965493631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG502.8199112E-547.5195162
TACCTGG10150.033.7084242
ATCACGC804.4824893E-429.6996943
GTATCAA163150.027.7587011
GTACATG122150.027.4276731
TACATGG123700.026.8137592
GTACCTG13200.026.6410371
ACATGGG123750.025.5357363
CGTCACT2059.513315E-1025.4995271
GAGTACT84850.025.25848612-13
CATGGGT6400.025.244744
ATGGGAT33950.024.9169525
CATGGGA97150.024.1632944
ATGGGAG30700.024.1490975
GGTATCA116050.023.8735351
GTTCGCA1000.001648623823.7620939
GTACACG2252.7976057E-923.2329041
TATCAAC194700.023.1862032
ATCAACG194800.023.1742973
TCAACGC195850.023.0985834
Produced by FastQC (version 0.11.5)