##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936490_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017957 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.472016990894506 31.0 31.0 34.0 30.0 34.0 2 31.450899203011524 31.0 31.0 34.0 28.0 34.0 3 31.467688713766886 31.0 31.0 34.0 28.0 34.0 4 35.16213946168649 37.0 35.0 37.0 33.0 37.0 5 35.10150625222873 37.0 35.0 37.0 32.0 37.0 6 35.27181501772668 37.0 35.0 37.0 32.0 37.0 7 35.128464168918725 37.0 35.0 37.0 32.0 37.0 8 35.114532342721745 37.0 35.0 37.0 32.0 37.0 9 36.5948335735203 39.0 37.0 39.0 32.0 39.0 10-11 36.476449398157285 39.0 37.0 39.0 32.0 39.0 12-13 36.3822224317923 39.0 37.0 39.0 32.0 39.0 14-15 37.351412682461046 39.5 37.0 41.0 32.0 41.0 16-17 37.33276847646806 39.0 37.0 41.0 32.0 41.0 18-19 37.411903449752785 39.5 37.0 41.0 32.0 41.0 20-21 37.3804045750459 40.0 37.0 41.0 32.0 41.0 22-23 37.27109789509773 39.0 36.0 41.0 31.5 41.0 24-25 37.12355040537076 39.0 36.0 41.0 31.0 41.0 26-27 36.90433092949898 39.0 36.0 41.0 30.5 41.0 28-29 36.96804481918195 39.0 36.0 41.0 31.0 41.0 30-31 36.80249902500793 39.0 35.5 41.0 31.0 41.0 32-33 36.547736299273936 39.0 35.0 40.5 30.0 41.0 34-35 36.32166093459743 38.5 35.0 40.0 30.0 41.0 36-37 36.026990825742146 38.0 35.0 40.0 30.0 41.0 38-39 35.799050942230366 38.0 35.0 40.0 29.0 41.0 40-41 35.587003675007885 38.0 35.0 40.0 28.5 41.0 42-43 35.219699358617305 38.0 35.0 40.0 27.0 41.0 44-45 34.77396982387272 38.0 34.0 40.0 25.0 41.0 46-47 34.17782578242499 38.0 33.5 40.0 22.5 41.0 48-49 33.700369465507876 38.0 33.0 40.0 19.5 41.0 50-51 32.872612497384466 37.0 32.0 39.5 16.5 40.5 52-53 33.00635832358341 37.5 33.0 39.5 15.0 40.5 54-55 33.32889208483266 38.0 33.0 40.0 14.5 41.0 56-57 33.24436886823314 37.5 33.0 40.0 13.5 41.0 58-59 33.01590636932602 37.0 33.0 40.0 11.0 41.0 60-61 32.685971018422194 36.5 32.5 40.0 8.0 41.0 62-63 32.366279223975084 36.0 32.0 39.5 7.5 41.0 64-65 32.05560942161604 36.0 31.5 39.0 6.5 41.0 66-67 31.685086894633073 35.0 31.0 39.0 2.0 41.0 68-69 31.324756350219115 35.0 31.0 38.5 2.0 40.0 70-71 30.92718602062759 35.0 31.0 37.5 2.0 40.0 72-73 30.529286109334677 35.0 30.0 37.0 2.0 39.5 74-75 30.12988662585944 35.0 30.0 36.5 2.0 39.0 76-77 29.733011806982024 34.5 29.5 36.0 2.0 39.0 78-79 29.30008045526481 34.0 29.0 36.0 2.0 37.5 80-81 28.905892390346548 34.0 29.0 35.0 2.0 37.0 82-83 28.51716870162492 34.0 28.5 35.0 2.0 37.0 84-85 28.13247710856156 34.0 27.5 35.0 2.0 36.0 86-87 27.802155199089942 34.0 27.0 35.0 2.0 36.0 88-89 27.424197682220367 34.0 26.0 35.0 2.0 35.5 90-91 27.047442082524114 33.5 25.5 35.0 2.0 35.0 92-93 26.68277343738488 33.0 25.0 35.0 2.0 35.0 94-95 26.277558875276657 33.0 24.5 35.0 2.0 35.0 96-97 25.72064930051073 33.0 21.5 35.0 2.0 35.0 98-99 25.116183198307986 33.0 17.5 35.0 2.0 35.0 100-101 23.202631840048255 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4086917204561793 1101 2 0.35387419477735094 1101 3 0.4845170615815526 1101 4 0.39817577997280296 1101 5 0.5357273183948692 1101 6 0.3086656163802033 1101 7 0.4455330817629459 1101 8 0.41165260462633313 1101 9 0.5044320112702891 1101 10-11 0.5883428310259475 1101 12-13 0.632168612939985 1101 14-15 0.7862720942628414 1101 16-17 0.8424207992396191 1101 18-19 0.9081110624813746 1101 20-21 0.9514787964471125 1101 22-23 0.996901753654889 1101 24-25 0.7746896545876538 1101 26-27 0.8853871742459916 1101 28-29 0.9737694080712984 1101 30-31 0.6079430610949501 1101 32-33 0.6708099161707892 1101 34-35 0.8911793714924059 1101 36-37 1.063461904136311 1101 38-39 0.8055634493206725 1101 40-41 0.6119004864955002 1101 42-43 0.5423541521251423 1101 44-45 0.8526647715007201 1101 46-47 0.8444168883726135 1101 48-49 0.49547732869618955 1101 50-51 0.9251369874795756 1101 52-53 0.653407059444369 1101 54-55 0.6403365191726138 1101 56-57 0.6275999643620551 1101 58-59 0.5701122626514916 1101 60-61 0.6667613435217774 1101 62-63 0.3569441005696987 1101 64-65 0.3899113330508186 1101 66-67 0.5428582621380258 1101 68-69 0.5069063535450056 1101 70-71 0.251036855119505 1101 72-73 0.2904142951731181 1101 74-75 0.3075448434092465 1101 76-77 0.2920380284302091 1101 78-79 0.08084806818610701 1101 80-81 0.18461078606901182 1101 82-83 0.5320547635610424 1101 84-85 0.35652402188183174 1101 86-87 0.481309544997341 1101 88-89 0.6277769371529907 1101 90-91 0.4493815808520232 1101 92-93 0.11358054338523615 1101 94-95 0.17982808549714946 1101 96-97 0.12236866114726297 1101 98-99 0.03395091182675358 1101 100-101 0.23609703565306717 1102 1 0.3393588105129197 1102 2 0.3154212537483474 1102 3 0.4210336197545139 1102 4 0.5120168749932361 1102 5 0.5414483071221099 1102 6 0.5612299814204391 1102 7 0.37955003686410294 1102 8 0.3357097957117432 1102 9 0.6941323513750746 1102 10-11 0.7945271811918175 1102 12-13 0.7324681892713656 1102 14-15 0.7342933027629499 1102 16-17 0.6441833693287862 1102 18-19 0.5835554131486305 1102 20-21 0.4000917413200611 1102 22-23 0.23630794907725772 1102 24-25 0.4818010409951654 1102 26-27 0.5888227966711881 1102 28-29 0.580683061565523 1102 30-31 0.42609145374466806 1102 32-33 0.4845863536775781 1102 34-35 0.5859295783128857 1102 36-37 0.44821014720677965 1102 38-39 0.31751568841350064 1102 40-41 0.15277260040971896 1102 42-43 0.4432777413587772 1102 44-45 0.3308661018368966 1102 46-47 0.7248642055558392 1102 48-49 0.6174136697703219 1102 50-51 0.7480485184525065 1102 52-53 0.7676273253444705 1102 54-55 0.5771403280119927 1102 56-57 0.8975743311571307 1102 58-59 0.7866162235770418 1102 60-61 0.5579596697382598 1102 62-63 0.5209856443279008 1102 64-65 0.5249698068410211 1102 66-67 0.5077964457946109 1102 68-69 0.7776813662884905 1102 70-71 0.6144120817863943 1102 72-73 0.399838519491869 1102 74-75 0.5068315216634289 1102 76-77 0.5556667668670663 1102 78-79 0.44356052922447375 1102 80-81 0.3974442057516363 1102 82-83 0.40834686928600306 1102 84-85 0.43641261040517776 1102 86-87 0.44212959864531953 1102 88-89 0.343356473206601 1102 90-91 0.2597183221968997 1102 92-93 0.32398330015599797 1102 94-95 0.5676462547807581 1102 96-97 0.4046399678348749 1102 98-99 0.19428587744024028 1102 100-101 0.4361645134459806 1103 1 0.313816334956627 1103 2 0.484157865785356 1103 3 0.44932226795028996 1103 4 0.4140429819543314 1103 5 0.45083748425074077 1103 6 0.17269557404607383 1103 7 0.400542957027028 1103 8 0.12464667137340513 1103 9 0.5553402117867137 1103 10-11 0.5961813218913221 1103 12-13 0.5907714249619005 1103 14-15 0.6081753490527433 1103 16-17 0.6541361407052122 1103 18-19 0.5460834764977704 1103 20-21 0.4632527217026947 1103 22-23 0.5895025906239937 1103 24-25 0.5941084103043863 1103 26-27 0.6469880800181684 1103 28-29 0.622507478833235 1103 30-31 0.5047708348704489 1103 32-33 0.5187050481512614 1103 34-35 0.4422600855873142 1103 36-37 0.4858232052428306 1103 38-39 0.4580983365241096 1103 40-41 0.5682641633315342 1103 42-43 0.49987533942328355 1103 44-45 0.5023023412144241 1103 46-47 0.48852896452546446 1103 48-49 0.6185376892242758 1103 50-51 0.22559189718566586 1103 52-53 0.2881528118666381 1103 54-55 0.385938181778549 1103 56-57 0.5630389077087941 1103 58-59 0.5094102487451764 1103 60-61 0.36320473073735826 1103 62-63 0.35669064220365243 1103 64-65 0.3223006815262224 1103 66-67 0.2754343907107213 1103 68-69 0.12157982227198616 1103 70-71 0.15318877229680083 1103 72-73 0.09001270784583681 1103 74-75 0.10139819978486031 1103 76-77 -0.006869318118706502 1103 78-79 0.18160358659699938 1103 80-81 0.49283285404644417 1103 82-83 0.24765120522818052 1103 84-85 0.13055602340359584 1103 86-87 0.369155482783178 1103 88-89 0.3011379063858719 1103 90-91 0.41125000914708565 1103 92-93 0.2708577833298733 1103 94-95 0.4752790896172421 1103 96-97 0.8175235085258628 1103 98-99 0.7167212067086162 1103 100-101 0.8971640093702931 1104 1 0.2615922674278721 1104 2 0.3883344095735346 1104 3 0.49695767323165185 1104 4 0.5774926642303981 1104 5 0.38542089447184935 1104 6 0.536715881881058 1104 7 0.49663800450818485 1104 8 0.5996351973520078 1104 9 0.5859647297595245 1104 10-11 0.7380503808323056 1104 12-13 0.7684304407037885 1104 14-15 0.7618520040324768 1104 16-17 0.6105916380626368 1104 18-19 0.7904422797928277 1104 20-21 0.6810826412189428 1104 22-23 0.629488490589452 1104 24-25 0.7629492265922906 1104 26-27 0.6133031921323706 1104 28-29 0.5417436165593443 1104 30-31 0.4260689843592118 1104 32-33 0.43369319560544994 1104 34-35 0.4529108163760256 1104 36-37 0.6611188091548783 1104 38-39 0.5610725921321844 1104 40-41 0.5823310769853833 1104 42-43 0.5774312072614833 1104 44-45 0.401689604814095 1104 46-47 0.45615282481210073 1104 48-49 0.13658005556967368 1104 50-51 -0.02558365770747173 1104 52-53 0.1545838470836074 1104 54-55 0.463130666176248 1104 56-57 0.6088427279664756 1104 58-59 0.6615179200156476 1104 60-61 0.5781933513470747 1104 62-63 0.4365569284532782 1104 64-65 0.5160157567342978 1104 66-67 0.3927868963057861 1104 68-69 0.3920393035989065 1104 70-71 0.5064466846400073 1104 72-73 0.28594120458342687 1104 74-75 0.14096251818067884 1104 76-77 0.038062003357349994 1104 78-79 0.13513450967610652 1104 80-81 0.3881755842484331 1104 82-83 0.21021182827937324 1104 84-85 0.27790233066132686 1104 86-87 0.29160718543332464 1104 88-89 0.13886259611414076 1104 90-91 0.19802862441013502 1104 92-93 0.08617855911566608 1104 94-95 0.07582511108903844 1104 96-97 0.11048200805507591 1104 98-99 -0.16058436185871727 1104 100-101 -0.06693626174781997 1105 1 0.29634076468051873 1105 2 0.4732672930045929 1105 3 0.2971392936173878 1105 4 0.3223446021291352 1105 5 0.47196162537865405 1105 6 0.27380977132310846 1105 7 0.47063402176673463 1105 8 0.21001706112750185 1105 9 0.6188490827484259 1105 10-11 0.6193227376564394 1105 12-13 0.538984428976832 1105 14-15 0.6654950139524516 1105 16-17 0.5667008131188709 1105 18-19 0.4623939152982288 1105 20-21 0.16723016860029105 1105 22-23 0.026655396958148003 1105 24-25 -0.006814666906251432 1105 26-27 0.015627379911258288 1105 28-29 0.1400956650897811 1105 30-31 0.12872645015557538 1105 32-33 -0.017940004969837275 1105 34-35 0.02702253596152815 1105 36-37 -0.03955604425811288 1105 38-39 -0.04959553118528959 1105 40-41 -0.19433807889469534 1105 42-43 0.03165687750524171 1105 44-45 0.15690819462860617 1105 46-47 0.02589597486557693 1105 48-49 0.07193149562572643 1105 50-51 0.03774423623359979 1105 52-53 0.04561023274573728 1105 54-55 0.0071312008961612605 1105 56-57 0.15140366759844426 1105 58-59 -0.03938004067322254 1105 60-61 -0.045133805049282216 1105 62-63 0.049940599213321946 1105 64-65 0.09601031734203502 1105 66-67 -0.12308552689736985 1105 68-69 -0.10095356065599148 1105 70-71 -0.021102915241986153 1105 72-73 -0.02525979478965823 1105 74-75 -0.05163990834142851 1105 76-77 0.06347595032788433 1105 78-79 -0.1864497363688109 1105 80-81 0.11901555548419296 1105 82-83 -0.16624897121909754 1105 84-85 -0.2303632407514371 1105 86-87 -0.14407292298574959 1105 88-89 -0.1742426515164972 1105 90-91 0.19567124711913664 1105 92-93 0.34877075220487086 1105 94-95 0.2199123073128071 1105 96-97 0.4066882624342476 1105 98-99 0.16070947595520124 1105 100-101 0.6704544894414717 1106 1 0.026737217601958463 1106 2 0.1558572398484941 1106 3 -0.04057452911453652 1106 4 0.26153524150470986 1106 5 0.2902749029565115 1106 6 0.26594814172739234 1106 7 0.41012156263540334 1106 8 0.4216210803867497 1106 9 0.42078627556269765 1106 10-11 0.2711301702097231 1106 12-13 0.34076109824287215 1106 14-15 0.31700822785745686 1106 16-17 0.27904216756202516 1106 18-19 0.3660006424421809 1106 20-21 0.2926062140154926 1106 22-23 0.3047742934861901 1106 24-25 0.13096148725358603 1106 26-27 0.051544412041003795 1106 28-29 0.142445390008767 1106 30-31 0.10585564523058366 1106 32-33 0.13656458090623858 1106 34-35 0.2899024267876271 1106 36-37 0.3908187670484651 1106 38-39 -0.04788611189599834 1106 40-41 -0.0020447294881407174 1106 42-43 0.3229731348294109 1106 44-45 0.0720457887964514 1106 46-47 0.4662751144951258 1106 48-49 0.3306140757354683 1106 50-51 0.33789152441406145 1106 52-53 0.26477495584037314 1106 54-55 0.39605561263772415 1106 56-57 0.3878293671055104 1106 58-59 0.2355346448615947 1106 60-61 0.1714527041761258 1106 62-63 0.1708179610110463 1106 64-65 -0.2015462370267116 1106 66-67 0.27276688444354846 1106 68-69 0.3760701091895484 1106 70-71 0.42588988400252603 1106 72-73 0.15689955546109857 1106 74-75 0.13324029369466928 1106 76-77 0.08606013799824197 1106 78-79 0.2676664630059058 1106 80-81 -0.009960867675555818 1106 82-83 0.1303337104810396 1106 84-85 0.28145692637190933 1106 86-87 0.32891753681807856 1106 88-89 0.33186976325846373 1106 90-91 0.511700529476034 1106 92-93 0.3587826819214932 1106 94-95 0.2538301729130161 1106 96-97 0.19058242957190075 1106 98-99 0.47555651802936083 1106 100-101 0.7702518452296196 1107 1 0.36923904463986545 1107 2 0.27265351128394144 1107 3 0.465291224173896 1107 4 0.23416672960011908 1107 5 0.3338301733159099 1107 6 0.4247517438299653 1107 7 0.38637992033705615 1107 8 0.5033476054122445 1107 9 0.5489538057920811 1107 10-11 0.6315482265040657 1107 12-13 0.6807688660856996 1107 14-15 0.7224169167712944 1107 16-17 0.7708606026794271 1107 18-19 0.7441351030508727 1107 20-21 0.676895845727806 1107 22-23 0.5910413477944871 1107 24-25 0.6998208808552491 1107 26-27 0.6938924770694754 1107 28-29 0.6845690506891273 1107 30-31 0.5883573596910594 1107 32-33 0.6293671304098183 1107 34-35 0.5896562574692936 1107 36-37 0.6598914948840857 1107 38-39 0.7640668907786576 1107 40-41 0.8954267813854315 1107 42-43 0.9842206053552331 1107 44-45 0.8756722831446027 1107 46-47 0.984968597106139 1107 48-49 0.8095269154730502 1107 50-51 0.743128532743853 1107 52-53 0.7477813924550603 1107 54-55 0.9553881410424196 1107 56-57 0.9658736719539434 1107 58-59 0.9479565325412125 1107 60-61 0.9805870493929447 1107 62-63 1.0418361206889912 1107 64-65 0.8947379571956233 1107 66-67 0.9696202514000163 1107 68-69 1.045549335369806 1107 70-71 1.1148159234440307 1107 72-73 0.9660822151592221 1107 74-75 0.9740651860659533 1107 76-77 0.9267724529373602 1107 78-79 1.1464327644526229 1107 80-81 1.2674679200625114 1107 82-83 1.2892996117149842 1107 84-85 1.284204284789368 1107 86-87 1.349691356560971 1107 88-89 1.4339141694265827 1107 90-91 1.3318885713135593 1107 92-93 1.2860262328763845 1107 94-95 1.515216676567558 1107 96-97 1.6553137963651245 1107 98-99 1.9170106244319207 1107 100-101 2.1585370376385242 1108 1 0.19322536862619089 1108 2 0.17372155520912713 1108 3 0.0034072622899365967 1108 4 0.11731054131536212 1108 5 0.09149135954852028 1108 6 0.23169226505620344 1108 7 0.3108349876492653 1108 8 0.4597214189289147 1108 9 0.4427436995819747 1108 10-11 0.2875121681822961 1108 12-13 0.33338298298552616 1108 14-15 0.5360612661299342 1108 16-17 0.6436731183490849 1108 18-19 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-2.337785844172455 2316 72-73 -2.3753996003167046 2316 74-75 -2.5198620090388673 2316 76-77 -2.937263811000765 2316 78-79 -3.1467161253596636 2316 80-81 -3.133506656083803 2316 82-83 -3.120882405434653 2316 84-85 -3.22175063971315 2316 86-87 -3.464187782637108 2316 88-89 -3.3147383103348282 2316 90-91 -3.3018182463971826 2316 92-93 -3.338683363864618 2316 94-95 -3.4383708658325425 2316 96-97 -3.728069095849957 2316 98-99 -4.06920179014163 2316 100-101 -4.729732979188903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1705.0 3 637.0 4 623.0 5 697.0 6 855.0 7 1051.0 8 1282.0 9 1790.0 10 2486.0 11 3655.0 12 5362.0 13 6894.0 14 8071.0 15 10428.0 16 13749.0 17 17955.0 18 14678.0 19 7789.0 20 7138.0 21 7697.0 22 8262.0 23 9087.0 24 10482.0 25 11880.0 26 13932.0 27 16470.0 28 19689.0 29 23421.0 30 28613.0 31 35082.0 32 43556.0 33 56153.0 34 73397.0 35 101444.0 36 134397.0 37 166118.0 38 130601.0 39 20824.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.82157605005978 18.52149145774855 13.998995896143043 19.657936596048625 2 15.678673353603726 21.654869560518907 40.0426365918234 22.623820494053966 3 19.544508697779428 29.48157186843589 29.346089683508115 21.627829750276565 4 11.97092299921185 17.035321264104624 37.59854320177205 33.395212534911465 5 12.280248266508101 39.883729501550434 31.960823820218092 15.875198411723368 6 28.399736177582763 36.68203009580765 18.606066146295625 16.31216758031397 7 27.613984749626603 35.475984592406256 20.341659460734217 16.568371197232924 8 27.966179136595215 31.700721714821928 19.83531735309001 20.497781795492845 9 29.609772296760905 16.17954522231916 20.32587422481077 33.88480825610916 10-11 25.004324324324323 27.71199017199017 25.601425061425058 21.682260442260443 12-13 27.87065851550357 25.73925811050064 26.597223553067817 19.79285982092797 14-15 24.378809211967816 25.634160408660954 27.739267393292938 22.24776298607829 16-17 20.731511400142573 29.627695402064504 27.712646349198238 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20.596524766539677 26.81792550881874 31.069673636911975 21.515876087729605 44-45 20.898475394560855 26.725907486279826 30.81462430576397 21.560992813395348 46-47 21.45104010609819 27.110690866222953 30.15168111599578 21.286587911683082 48-49 21.553998829016805 27.511179352972842 29.261956406417617 21.672865411592735 50-51 21.489131678775326 27.856185386991612 29.11156944619772 21.543113488035342 52-53 21.704990760761184 28.268976296268644 28.344570068402785 21.681462874567387 54-55 21.07425959963809 27.865548579517824 28.740658729636348 22.319533091207735 56-57 21.813519018902735 28.178548149341577 27.927055278820617 22.080877552935075 58-59 22.083225065856915 27.518588031650616 28.228953662175023 22.16923324031745 60-61 21.77017335407446 27.958962439541214 28.837742295633706 21.433121910750625 62-63 21.946894656752516 28.082576657343907 28.806883320120363 21.163645365783214 64-65 21.523348450829168 28.3612932507659 28.7034139867209 21.411944311684024 66-67 21.72232305345911 28.430217279717855 28.120568508071546 21.72689115875148 68-69 21.68724708388145 28.317173882502505 28.499121210126567 21.49645782348948 70-71 21.833585339805918 28.345914007335203 28.199769902862066 21.62073074999681 72-73 21.724447561983187 28.33376068502019 28.15437121830598 21.78742053469064 74-75 21.120869620744365 29.405205739182545 27.93843297328362 21.535491666789465 76-77 21.26078546370494 28.82458461904215 28.198406359629086 21.716223557623824 78-79 21.127164037121716 28.702012585847775 28.314275704459096 21.85654767257141 80-81 21.19546606649186 29.371275622025472 27.706498012436825 21.726760299045843 82-83 21.296680615120426 28.877965683758312 27.77374720568378 22.051606495437483 84-85 20.970194086957207 28.803966327673958 27.697438323319762 22.528401262049076 86-87 21.280253785238468 28.98866602120018 27.479679349542696 22.251400844018654 88-89 21.18820363114071 28.61332197650138 27.49095063855163 22.70752375380628 90-91 21.027842266129976 28.42354552430095 27.869284102330326 22.679328107238753 92-93 21.231150594810003 28.818269529300338 27.764677678610028 22.185902197279635 94-95 20.83581805088767 28.430506666247496 28.027319285305175 22.706355997559655 96-97 20.74379747158766 28.42098748270356 28.233053659380015 22.602161386328774 98-99 20.779112949859073 28.697585734407692 27.605243674227182 22.918057641506053 100-101 20.718237609759957 28.53790818615544 28.075256669626526 22.66859753445808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 956.0 1 747.0 2 1121.0 3 1573.5 4 1243.5 5 1132.5 6 1651.0 7 2967.5 8 4453.0 9 5034.5 10 4266.0 11 3324.0 12 3140.5 13 3253.5 14 3375.5 15 3349.0 16 3288.0 17 3255.0 18 3277.0 19 3375.5 20 3555.5 21 3827.5 22 4172.5 23 4581.5 24 4981.0 25 5686.5 26 6621.0 27 7716.5 28 8769.5 29 10051.0 30 12117.0 31 14455.5 32 17632.5 33 20347.5 34 22283.5 35 24046.0 36 26564.5 37 29141.0 38 28962.0 39 27976.5 40 27466.5 41 27542.5 42 27647.0 43 28633.5 44 30154.0 45 30955.5 46 31018.5 47 33691.0 48 43963.5 49 44293.5 50 35368.0 51 30854.0 52 27670.5 53 27198.0 54 26920.5 55 25260.0 56 23537.0 57 22063.0 58 21285.5 59 20500.5 60 18850.0 61 17490.0 62 15438.5 63 11234.5 64 7578.0 65 5707.5 66 4196.0 67 3195.5 68 2341.5 69 1537.0 70 1160.0 71 988.0 72 925.5 73 968.0 74 809.5 75 615.5 76 433.0 77 240.0 78 150.5 79 100.0 80 61.0 81 36.5 82 31.0 83 16.5 84 7.5 85 5.5 86 3.0 87 2.5 88 3.0 89 3.0 90 3.0 91 3.0 92 1.5 93 1.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013163620860213152 2 0.005108270781575253 3 0.010904193399131791 4 0.03762437902583312 5 0.04931446023751495 6 0.05953100180066545 7 0.027211365509545095 8 0.023773106329638676 9 0.013851272696194437 10-11 0.04489384129192098 12-13 0.042585295842555236 14-15 0.019057779454338446 16-17 0.02244692064596049 18-19 0.031140804572295292 20-21 0.025590471896160646 22-23 0.014293334590753833 24-25 0.002848843320493891 26-27 0.0029961972853470233 28-29 0.0013261856836781908 30-31 0.006974754336381596 32-33 0.002062955507943852 34-35 0.002062955507943852 36-37 0.006876518359812841 38-39 0.006188866523831557 40-41 0.004911798828437743 42-43 0.0010805957422563035 44-45 0.0021120734962282294 46-47 0.003143551250200156 48-49 8.841237891187939E-4 50-51 0.0021120734962282294 52-53 0.001571775625100078 54-55 0.0027506073439251366 56-57 0.003487377168190798 58-59 0.0029961972853470233 60-61 0.001129713730540681 62-63 0.0017682475782375877 64-65 0.004076793027603327 66-67 0.0028979613087782687 68-69 0.00785887812550039 70-71 0.012377733047663113 72-73 0.0058941585941252915 74-75 0.004125911015887704 76-77 0.0035364951564751754 78-79 0.037869968967255 80-81 0.024902820060179357 82-83 0.004175029004172081 84-85 0.008693883926334805 86-87 0.008693883926334805 88-89 0.006630928418390953 90-91 0.011395373281975565 92-93 0.015030104415019495 94-95 0.006680046406675331 96-97 0.004469736933878346 98-99 0.02313457248194177 100-101 0.04445177939736158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1017957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.01705156944076 #Duplication Level Percentage of deduplicated Percentage of total 1 76.94284152372865 36.94568389344186 2 12.51524260880578 12.01890099502179 3 4.152565344567296 5.9818183288668125 4 1.9720652649966617 3.787710381105903 5 1.0507953261561305 2.5228046682483116 6 0.6212038801654755 1.78970272494254 7 0.44973877318982314 1.511659090552293 8 0.3115734363681815 1.1968670209407073 9 0.23649259434144598 1.0220109388455643 >10 1.5060782577868543 14.043998649013895 >50 0.15203626575045606 5.106414290467873 >100 0.08114909439802241 7.468138610954327 >500 0.004725137103543489 1.6049799837431575 >1k 0.0028761704108525585 2.5172636278523597 >5k 4.1088148726465125E-4 1.3783887715330756 >10k+ 2.0544074363232562E-4 1.1036580244694805 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11188 1.0990641058512294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8591 0.8439452747021731 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5382 0.5287060258930387 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3990 0.3919615465093319 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2431 0.23881165903864307 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2322 0.22810393759264883 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2238 0.2198521155608734 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2108 0.20708143860693526 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1741 0.1710288352062022 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1724 0.1693588236045334 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1524 0.14971162829078244 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1434 0.14087039039959448 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1374 0.1349762318054692 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1339 0.13153797262556277 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1166 0.11454314867916818 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1088 0.10688074250680529 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1039 0.10206717965493631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.823597656875487E-5 0.0 5 0.0 0.0 0.0 9.823597656875487E-5 0.0 6 0.0 0.0 0.0 9.823597656875487E-5 0.0 7 0.0 0.0 0.0 9.823597656875487E-5 0.0 8 0.0 0.0 0.0 9.823597656875487E-5 0.0 9 0.0 0.0 0.0 9.823597656875487E-5 0.0 10-11 0.0 0.0 0.0 2.4558994142188717E-4 0.0 12-13 0.0 0.0 0.0 3.929439062750195E-4 0.0 14-15 0.0 0.0 0.0 4.911798828437743E-4 0.0 16-17 0.0 0.0 0.0 6.87651835981284E-4 0.0 18-19 0.0 0.0 0.0 8.350058008344164E-4 0.0 20-21 0.0 0.0 0.0 9.823597656875487E-4 0.0 22-23 0.0 0.0 0.0 0.0010805957422563035 0.0 24-25 0.0 0.0 0.0 0.0012279497071094358 0.0 26-27 0.0 0.0 0.0 0.001473539648531323 0.0 28-29 0.0 0.0 0.0 0.0028979613087782687 0.0 30-31 0.0 0.0 0.0 0.0049609168167221215 0.0 32-33 0.0 0.0 0.0 0.006925636348097218 0.0 34-35 0.0 0.0 0.0 0.00898859185604107 0.0 36-37 0.0 0.0 0.0 0.01203390712967247 0.0 38-39 0.0 0.0 0.0 0.015619520274432024 0.0 40-41 0.0 0.0 0.0 0.020187493184879127 0.0 42-43 0.0 0.0 0.0 0.027751663380673253 0.0 44-45 0.0 0.0 0.0 0.03654378328357681 0.0 46-47 0.0 0.0 0.0 0.06036600760149986 0.0 48-49 0.0 0.0 0.0 0.10516161291685208 0.0 50-51 0.0 0.0 0.0 0.1392986147744944 0.0 52-53 0.0 0.0 0.0 0.17358297059698985 0.0 54-55 0.0 0.0 0.0 0.21543149661527944 0.0 56-57 0.0 0.0 0.0 0.24858613870723417 0.0 58-59 0.0 0.0 0.0 0.29397115988199896 0.0 60-61 0.0 0.0 0.0 0.37270729510185596 0.0 62-63 0.0 0.0 0.0 0.438427163426353 0.0 64-65 0.0 0.0 0.0 0.5085185327081596 0.0 66-67 0.0 0.0 0.0 0.627777008262628 0.0 68-69 0.0 0.0 0.0 0.7662406172362879 0.0 70-71 0.0 0.0 0.0 0.8860393906618845 0.0 72-73 0.0 0.0 0.0 1.0143846940489627 0.0 74-75 0.0 0.0 0.0 1.130892562259506 0.0 76-77 0.0 0.0 0.0 1.2221537844918793 0.0 78-79 0.0 0.0 0.0 1.3370898770773225 0.0 80-81 0.0 0.0 0.0 1.5362142015821887 0.0 82-83 0.0 0.0 0.0 1.7515474622208993 0.0 84-85 0.0 0.0 0.0 1.9949270941699897 0.0 86-87 0.0 0.0 0.0 2.3719076542525865 0.0 88-89 0.0 0.0 0.0 2.8206495952186588 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 50 2.8199112E-5 47.519516 2 TACCTGG 1015 0.0 33.708424 2 ATCACGC 80 4.4824893E-4 29.699694 3 GTATCAA 16315 0.0 27.758701 1 GTACATG 12215 0.0 27.427673 1 TACATGG 12370 0.0 26.813759 2 GTACCTG 1320 0.0 26.641037 1 ACATGGG 12375 0.0 25.535736 3 CGTCACT 205 9.513315E-10 25.499527 1 GAGTACT 8485 0.0 25.258486 12-13 CATGGGT 640 0.0 25.24474 4 ATGGGAT 3395 0.0 24.916952 5 CATGGGA 9715 0.0 24.163294 4 ATGGGAG 3070 0.0 24.149097 5 GGTATCA 11605 0.0 23.873535 1 GTTCGCA 100 0.0016486238 23.762093 9 GTACACG 225 2.7976057E-9 23.232904 1 TATCAAC 19470 0.0 23.186203 2 ATCAACG 19480 0.0 23.174297 3 TCAACGC 19585 0.0 23.098583 4 >>END_MODULE