FastQCFastQC Report
Thu 26 May 2016
SRR936490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017957
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127031.2478916103528932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107341.0544649724890147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72520.7124073020766103No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44760.4397042311217468No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36360.3571860108039927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28620.28115136493977644No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.2653353727122069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.21022498985713542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20280.19922256048143486No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.171520015089046No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16830.16533114856521444No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15250.14980986426735118No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14760.1449963014154822No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14650.14391570567322587No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12300.12083025117956848No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12010.1179814078590746No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11240.11041723766328046No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.11012252973357421No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10920.10727368641308031No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.10550543883484273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG802.2609674E-741.565512
ATCACGC951.9283107E-839.9989663
TACCTGG9100.037.063122
TAACGCA1302.055458E-1036.535724
TCACGCA1252.1549204E-730.397724
GTACCTG11550.029.5027541
TATCACG1301.0013973E-525.5787752
ATAACGC1701.122844E-725.1464083
GTACATG106950.025.1300541
ACCTGGG13100.024.6558533
TACATGG109500.024.5976722
GTATCAA177700.024.550631
ACATGGG108700.023.7712333
GGTATCA132800.023.707841
GAGTACT108150.023.53614412-13
TAAGGTG3250.023.3805645
CATGGGA85000.022.0718754
ATGGGAT28900.022.0204075
ATGGGAG27550.021.892765
AGTACTT110700.021.5354112-13
Produced by FastQC (version 0.11.5)