##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936490_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017957 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41477783442719 31.0 31.0 34.0 30.0 34.0 2 31.57461071538385 31.0 31.0 34.0 30.0 34.0 3 31.818258531548974 31.0 31.0 34.0 30.0 34.0 4 35.68464679745804 37.0 35.0 37.0 33.0 37.0 5 35.52131082157694 37.0 35.0 37.0 33.0 37.0 6 35.64884764287686 37.0 35.0 37.0 33.0 37.0 7 35.576585258512885 37.0 35.0 37.0 33.0 37.0 8 35.57111842641683 37.0 35.0 37.0 33.0 37.0 9 37.14831078326491 39.0 37.0 39.0 33.0 39.0 10-11 37.04017753205685 39.0 37.0 39.0 33.0 39.0 12-13 36.89737533117804 39.0 37.0 39.0 33.0 39.0 14-15 37.8613571103691 39.5 37.0 41.0 33.0 41.0 16-17 37.8259764410481 39.0 37.0 41.0 32.5 41.0 18-19 38.00833286671244 40.0 37.0 41.0 33.0 41.0 20-21 38.113482200132225 40.0 37.0 41.0 33.0 41.0 22-23 37.994032164423444 40.0 37.0 41.0 33.0 41.0 24-25 37.86560778107523 40.0 37.0 41.0 33.0 41.0 26-27 37.617056024959794 39.5 36.5 41.0 32.0 41.0 28-29 37.4729448296932 39.0 36.0 41.0 32.0 41.0 30-31 37.28245200926955 39.0 36.0 41.0 32.0 41.0 32-33 36.932495183981246 39.0 35.0 41.0 31.0 41.0 34-35 36.65678805686291 39.0 35.0 41.0 31.0 41.0 36-37 36.3127037782539 38.5 35.0 40.5 30.0 41.0 38-39 36.09435712903394 38.0 35.0 40.0 30.0 41.0 40-41 35.76103165457873 38.0 35.0 40.0 29.5 41.0 42-43 35.35912420662169 38.0 35.0 40.0 27.5 41.0 44-45 34.84028009041639 38.0 34.0 40.0 25.0 41.0 46-47 34.11187997135439 38.0 33.5 40.0 21.5 41.0 48-49 33.420984383426806 38.0 33.0 40.0 15.5 41.0 50-51 33.11479954457801 38.0 33.0 40.0 10.5 41.0 52-53 32.80839858658077 37.0 33.0 40.0 7.5 41.0 54-55 32.51901701152406 37.0 32.0 40.0 6.0 41.0 56-57 32.234154782569405 37.0 32.0 40.0 2.0 41.0 58-59 31.881379075933463 36.0 31.0 39.5 2.0 41.0 60-61 31.489666557624734 36.0 31.0 39.0 2.0 40.5 62-63 31.188695593232328 35.0 30.5 39.0 2.0 40.0 64-65 30.749600425165305 35.0 30.0 38.5 2.0 40.0 66-67 30.340364082176357 35.0 29.0 38.0 2.0 40.0 68-69 29.894575605845827 34.5 29.0 37.5 2.0 39.5 70-71 29.42532051943255 34.0 28.5 37.0 2.0 39.0 72-73 29.029148087787597 34.0 28.0 36.0 2.0 39.0 74-75 28.690580741622682 34.0 27.5 36.0 2.0 38.5 76-77 27.296443268232352 32.0 26.0 34.5 2.0 36.5 78-79 28.066951747470668 33.5 27.0 35.0 2.0 37.0 80-81 27.902193314648848 34.0 27.0 35.0 2.0 37.0 82-83 27.561501615490634 33.5 26.0 35.0 2.0 36.0 84-85 27.11587571970132 33.0 26.0 35.0 2.0 36.0 86-87 26.727181992952552 33.0 25.0 35.0 2.0 35.5 88-89 26.291416042131445 33.0 24.5 35.0 2.0 35.0 90-91 25.8126836398787 32.0 23.5 35.0 2.0 35.0 92-93 25.355785165778123 32.0 20.0 34.0 2.0 35.0 94-95 24.81761312118292 32.0 18.5 34.0 2.0 35.0 96-97 24.143578265093712 31.5 4.5 34.0 2.0 35.0 98-99 23.32421506998822 31.0 2.0 34.0 2.0 35.0 100-101 20.704511094280015 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5497828973552075 1101 2 0.5480458304602358 1101 3 0.2959658068104716 1101 4 0.3464542252005103 1101 5 0.38207002568550763 1101 6 0.35201782292260475 1101 7 0.27432623161774217 1101 8 0.29079184722111506 1101 9 0.2893267619512443 1101 10-11 0.40213357201355393 1101 12-13 0.40738791454050016 1101 14-15 0.528774873411642 1101 16-17 0.5402996520088621 1101 18-19 0.4894021247593159 1101 20-21 0.249437861093611 1101 22-23 0.331645673302269 1101 24-25 0.23830504579897394 1101 26-27 0.30075562187247584 1101 28-29 0.2673493629496022 1101 30-31 0.10347728968695691 1101 32-33 -0.007455911869527654 1101 34-35 0.3255856643335733 1101 36-37 0.14158380823297279 1101 38-39 -0.04978698130468473 1101 40-41 -0.005285418453091495 1101 42-43 -0.22220464467560674 1101 44-45 -0.24982913547146524 1101 46-47 0.1409800335807958 1101 48-49 0.009704934946462629 1101 50-51 -0.12261350812197946 1101 52-53 -0.22343723468113552 1101 54-55 -0.2204197392608549 1101 56-57 -0.18658200717608509 1101 58-59 -0.25996413352533665 1101 60-61 -0.12047890952218765 1101 62-63 -0.19299309304463108 1101 64-65 -0.059084158816329335 1101 66-67 -0.421314260963932 1101 68-69 -0.42502764404392224 1101 70-71 -0.43285948682653697 1101 72-73 -0.36921597662657035 1101 74-75 -0.6184395588580465 1101 76-77 -0.23868079256369157 1101 78-79 -0.2247315925362443 1101 80-81 -0.21887762136966415 1101 82-83 -0.027515962927811444 1101 84-85 0.17872387825629232 1101 86-87 -0.07281683878094114 1101 88-89 -0.1616979463898609 1101 90-91 0.019554678116854518 1101 92-93 -0.13861504816464176 1101 94-95 -0.22800417799208006 1101 96-97 -0.12044682815195884 1101 98-99 -0.32695689270584793 1101 100-101 -0.34651676848185886 1102 1 0.4841675333093818 1102 2 0.5770215885930874 1102 3 0.21259730703192048 1102 4 0.2538278975801589 1102 5 0.28383343141297246 1102 6 0.3002092927268194 1102 7 0.19340824116073208 1102 8 -0.024903947011303273 1102 9 0.21305074226360432 1102 10-11 0.19387318294035794 1102 12-13 0.4002797662314137 1102 14-15 0.4114914708596089 1102 16-17 0.4545793526832256 1102 18-19 0.4957002261890864 1102 20-21 0.4495361530489177 1102 22-23 0.4285854613843938 1102 24-25 0.32017827529032417 1102 26-27 0.012186736029022427 1102 28-29 0.1154391982452907 1102 30-31 0.21854259482028482 1102 32-33 0.3694373311591619 1102 34-35 0.44822759749921914 1102 36-37 0.5723208829975306 1102 38-39 0.11751302955861576 1102 40-41 0.42787239601849336 1102 42-43 0.3803438432368864 1102 44-45 0.4449268163623472 1102 46-47 0.3354581356790902 1102 48-49 0.10811179052308972 1102 50-51 0.09126184806884652 1102 52-53 0.254664341332699 1102 54-55 0.46096965565345016 1102 56-57 0.5039398698346673 1102 58-59 0.34564853558696385 1102 60-61 0.4492164585347034 1102 62-63 0.29858785105952634 1102 64-65 0.07884368799445696 1102 66-67 0.2719166509681479 1102 68-69 0.16492725237871042 1102 70-71 0.34572561039998106 1102 72-73 0.24009125832985134 1102 74-75 -0.03684261228040242 1102 76-77 0.30969169738775904 1102 78-79 0.33133451480985343 1102 80-81 0.3872975367748843 1102 82-83 0.36974544144591803 1102 84-85 0.562204040202996 1102 86-87 0.17493633873370484 1102 88-89 0.189397053150536 1102 90-91 0.33146659766159914 1102 92-93 0.7947041489540752 1102 94-95 0.5544112763026803 1102 96-97 0.5130672816007724 1102 98-99 0.1252362400185838 1102 100-101 -0.20018240034976387 1103 1 0.48297815237176067 1103 2 0.44626990182116444 1103 3 0.3192890355361584 1103 4 0.18138756716795257 1103 5 0.22110423295273307 1103 6 0.10754938734251596 1103 7 0.29832332569986875 1103 8 0.2330134578605012 1103 9 0.21604845758265157 1103 10-11 0.1103615084880829 1103 12-13 0.13659421042607534 1103 14-15 0.207584438657193 1103 16-17 0.23116290793200278 1103 18-19 0.33838899956200663 1103 20-21 0.1838649945958437 1103 22-23 0.13185521912106424 1103 24-25 0.18896262101691974 1103 26-27 0.2366534209836999 1103 28-29 0.20150937865288654 1103 30-31 0.1958413631524465 1103 32-33 0.19053453259829922 1103 34-35 0.11083458979152283 1103 36-37 0.3083412005156987 1103 38-39 0.155024579229881 1103 40-41 0.3635562009283291 1103 42-43 0.4050881164320401 1103 44-45 0.24427201313507396 1103 46-47 0.10420276935272454 1103 48-49 0.13074369940522246 1103 50-51 -0.3794250119951883 1103 52-53 -0.28534431411225825 1103 54-55 -0.011969204620534413 1103 56-57 -0.14761063330021074 1103 58-59 -0.06793021361210805 1103 60-61 0.028288503725235614 1103 62-63 -0.04858759203686702 1103 64-65 -0.054990882090386606 1103 66-67 0.12427991424915419 1103 68-69 -0.032827382592255816 1103 70-71 3.570161967516583E-4 1103 72-73 -0.06272106805370825 1103 74-75 -0.23454575032484826 1103 76-77 -0.3608047230806868 1103 78-79 -0.020808401045162128 1103 80-81 -0.06002853483159143 1103 82-83 0.21952246965732414 1103 84-85 0.38442229011956286 1103 86-87 0.41934669397336677 1103 88-89 0.22349148464922663 1103 90-91 -0.0563052590699904 1103 92-93 -0.014446755856827309 1103 94-95 -0.11441941665778543 1103 96-97 -0.3691334989737918 1103 98-99 -0.1937258196207985 1103 100-101 -0.27173440877941246 1104 1 0.41406305066698224 1104 2 0.483818577695434 1104 3 0.2971046657556542 1104 4 0.19482329135613696 1104 5 0.03316496719546791 1104 6 0.23727254404689546 1104 7 0.10184549540183241 1104 8 0.03268508792859137 1104 9 0.11724128265232991 1104 10-11 0.11690430303922739 1104 12-13 0.18436020663137498 1104 14-15 0.38858393238079003 1104 16-17 0.25255710799162046 1104 18-19 0.18518455379273036 1104 20-21 0.047165632882645525 1104 22-23 0.12561950250336906 1104 24-25 0.12085235685419349 1104 26-27 0.25256422170478743 1104 28-29 0.14395284839695677 1104 30-31 0.32795726732899055 1104 32-33 0.4674038517748187 1104 34-35 0.6202082738277284 1104 36-37 0.38466781030907526 1104 38-39 0.5797644619819451 1104 40-41 0.532411211881886 1104 42-43 0.6150702784688846 1104 44-45 0.7196262883317885 1104 46-47 1.0558041642152034 1104 48-49 1.2489611332935695 1104 50-51 1.0773645331604058 1104 52-53 0.8857619708125313 1104 54-55 1.1611494107371527 1104 56-57 1.2424748659025227 1104 58-59 1.1911153370699417 1104 60-61 0.76536071198937 1104 62-63 0.9230008383643202 1104 64-65 1.0085032768047029 1104 66-67 0.9106875755833563 1104 68-69 1.1909917395149883 1104 70-71 1.0972479112650504 1104 72-73 1.0686273778669815 1104 74-75 1.0156234027740858 1104 76-77 0.8257685426860384 1104 78-79 0.7366260550862265 1104 80-81 0.8747034462232577 1104 82-83 0.7861593275261356 1104 84-85 0.9141757287773125 1104 86-87 0.6494223515412543 1104 88-89 0.708608377720811 1104 90-91 0.6076715184570141 1104 92-93 0.7515404594809816 1104 94-95 0.7014734041534858 1104 96-97 0.22733534136933997 1104 98-99 0.3517950536350334 1104 100-101 0.3418915239284068 1105 1 0.4393009088371329 1105 2 0.5843763458851292 1105 3 0.32045164366618906 1105 4 0.1982537962054991 1105 5 0.08056696594164237 1105 6 -0.025758027181488785 1105 7 0.10764905036734973 1105 8 0.09000798500422036 1105 9 0.19013743635664326 1105 10-11 0.23603206422224332 1105 12-13 0.09691460055400825 1105 14-15 0.2407449383917779 1105 16-17 0.40324993905699813 1105 18-19 0.20215684055010996 1105 20-21 0.15366708953344954 1105 22-23 0.217477008864698 1105 24-25 -0.004183598450595127 1105 26-27 0.08664846774970414 1105 28-29 0.3022170369133903 1105 30-31 0.21918371199470243 1105 32-33 0.1836243038897294 1105 34-35 0.12769988425660728 1105 36-37 -0.09896883828775316 1105 38-39 0.06054911736417523 1105 40-41 0.1542904758122532 1105 42-43 0.1741347029996163 1105 44-45 -0.026633438039119994 1105 46-47 0.11333587863789774 1105 48-49 0.22357945390425726 1105 50-51 -0.058031620543907536 1105 52-53 0.2077421861755866 1105 54-55 0.25938437404430204 1105 56-57 0.18451445361503005 1105 58-59 0.17972027124407575 1105 60-61 0.3548617629905877 1105 62-63 0.2222370186202518 1105 64-65 0.19513481790438192 1105 66-67 -0.014715308216469936 1105 68-69 -0.1309649666103212 1105 70-71 -0.003485945725028472 1105 72-73 -0.012282660116571975 1105 74-75 -0.21420244448040648 1105 76-77 -0.03717536703848978 1105 78-79 -0.04329982167005042 1105 80-81 -0.17479917206047446 1105 82-83 0.09375004552591548 1105 84-85 0.1898312216415654 1105 86-87 -0.03113965753054515 1105 88-89 -0.15705707199707675 1105 90-91 -0.44975325198219096 1105 92-93 -0.10850259469167511 1105 94-95 0.047427971399127244 1105 96-97 -0.10960366368284369 1105 98-99 0.0361392841915098 1105 100-101 0.10345484214207445 1106 1 0.2360208627637448 1106 2 0.33338279902119794 1106 3 0.16892099126987503 1106 4 0.0710431320762055 1106 5 -0.0849466736414044 1106 6 0.06324087821782598 1106 7 -0.033067054571226606 1106 8 0.012526532404727675 1106 9 0.06956234174165132 1106 10-11 -0.16594707039932644 1106 12-13 0.004591203285492895 1106 14-15 0.16978727010586425 1106 16-17 0.10574972442941544 1106 18-19 0.0823933064922926 1106 20-21 -0.043625741189764256 1106 22-23 0.0044698617661040885 1106 24-25 -0.08635734663520367 1106 26-27 -0.05297616648911685 1106 28-29 -0.04352117466648764 1106 30-31 0.03819229352098574 1106 32-33 -0.11534655683843198 1106 34-35 -0.36993384661470685 1106 36-37 -0.060522777777080705 1106 38-39 -0.08939463232282918 1106 40-41 0.15986006165255873 1106 42-43 0.1874720188670338 1106 44-45 0.2916422797112901 1106 46-47 0.23418954197306618 1106 48-49 0.25420949318110075 1106 50-51 0.5566273541085707 1106 52-53 0.7943430754492269 1106 54-55 0.5563918395071497 1106 56-57 0.6127970931050726 1106 58-59 0.5446122062548504 1106 60-61 0.7172032068618925 1106 62-63 0.4488233847606047 1106 64-65 0.571863911389002 1106 66-67 0.8594431727925631 1106 68-69 0.49629488300728397 1106 70-71 0.5071166569921566 1106 72-73 0.5623625783704469 1106 74-75 0.6318053930035887 1106 76-77 0.3214655395484307 1106 78-79 0.5351197323338219 1106 80-81 0.3261402707663166 1106 82-83 0.5179279324457973 1106 84-85 0.9153207513925281 1106 86-87 0.9206120612786997 1106 88-89 0.8540588483617775 1106 90-91 0.7072463795737853 1106 92-93 0.9265527971109826 1106 94-95 0.9511621336718861 1106 96-97 0.7614650743784992 1106 98-99 0.8457874201509306 1106 100-101 1.2037462885173724 1107 1 0.5674338127333947 1107 2 0.6116507577015291 1107 3 0.4394905298806506 1107 4 0.23744852528572125 1107 5 0.12453459769553632 1107 6 0.24555909146387478 1107 7 0.24668598806841402 1107 8 0.23283306198960219 1107 9 0.32443108114647146 1107 10-11 0.3976502663938817 1107 12-13 0.44778885266871526 1107 14-15 0.551259651578242 1107 16-17 0.512791255182286 1107 18-19 0.3548672799971939 1107 20-21 0.47069786076269793 1107 22-23 0.47326715015101684 1107 24-25 0.34568153385728095 1107 26-27 0.2685932942556022 1107 28-29 0.5073553813647038 1107 30-31 0.4912597001966432 1107 32-33 0.4188117814436225 1107 34-35 0.2149073207006822 1107 36-37 0.4050838785512241 1107 38-39 0.3523413867327818 1107 40-41 0.27698186949601933 1107 42-43 0.16089436342340235 1107 44-45 0.12702499138266177 1107 46-47 0.39614015237728495 1107 48-49 0.17658876092119158 1107 50-51 0.14795251244403573 1107 52-53 -0.028665500136675348 1107 54-55 -0.0620128819903627 1107 56-57 -0.18666731976140483 1107 58-59 -0.4038709535685179 1107 60-61 -0.3964315150202502 1107 62-63 -0.29083473634081614 1107 64-65 -0.3394011493605902 1107 66-67 -0.14066377576799383 1107 68-69 -0.16183935244653824 1107 70-71 -0.16382185415254114 1107 72-73 -0.13599463545068247 1107 74-75 0.2712921149645382 1107 76-77 0.0831911827173748 1107 78-79 0.2318168981031583 1107 80-81 0.18036563014631213 1107 82-83 0.13148291198015372 1107 84-85 0.1737483448716688 1107 86-87 0.5083769583219464 1107 88-89 0.5326498104578938 1107 90-91 0.5729846576831967 1107 92-93 0.6661246088477419 1107 94-95 0.7927821041578369 1107 96-97 0.9151797074745751 1107 98-99 1.0627799515764487 1107 100-101 1.0128886079689714 1108 1 0.45404584220256794 1108 2 0.35948114838906164 1108 3 0.20149372045526803 1108 4 0.13571042354762142 1108 5 -0.06537052078100913 1108 6 0.014123574314076848 1108 7 -0.04147322866223391 1108 8 0.045472231771633176 1108 9 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2316 90-91 -1.555354181646809 2316 92-93 -1.5491161471192179 2316 94-95 -1.5708121083078161 2316 96-97 -1.337638370662443 2316 98-99 -1.8288182216703959 2316 100-101 -2.2486870010060507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 30.0 5 60.0 6 207.0 7 365.0 8 779.0 9 1465.0 10 2523.0 11 4084.0 12 5967.0 13 8173.0 14 9695.0 15 13359.0 16 17261.0 17 22070.0 18 17468.0 19 9543.0 20 7596.0 21 7869.0 22 8300.0 23 9146.0 24 10350.0 25 12283.0 26 14747.0 27 17849.0 28 21874.0 29 26666.0 30 32026.0 31 39299.0 32 48061.0 33 59781.0 34 77756.0 35 103068.0 36 136405.0 37 166692.0 38 99457.0 39 5671.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.30564310609008 19.351535023301235 15.097968386538478 19.244853484070212 2 15.828420969897037 22.546176216301188 39.25587518666981 22.369527627131966 3 19.580058217728016 29.0726130092946 30.318409514462573 21.028919258514808 4 12.203597650297544 17.76945288559692 37.02392636891586 33.00302309518967 5 11.972804352246706 40.00247554660953 32.245369892834375 15.77935020830939 6 28.27074228086255 37.345683560307556 17.9188315420003 16.464742616829593 7 26.798479700026622 35.82597300278892 20.419526561534525 16.956020735649936 8 28.00216512092358 31.896533940038722 19.524105635110324 20.577195303927375 9 28.778027963124515 15.985664772593308 20.648445052224552 34.58786221205762 10-11 24.959084675565546 27.831065389859678 25.53867750668988 21.671172427884898 12-13 27.92149743505109 25.756959548270352 26.96359560451651 19.357947412162044 14-15 24.017541033207724 25.65105957428415 28.454029447245265 21.87736994526286 16-17 20.11592827594879 29.333213485441917 28.7711072275155 21.77975101109379 18-19 20.051681947272822 26.03096201509494 34.17732772602379 19.740028311608445 20-21 22.005497285248786 24.24945258002057 34.275858410522254 19.46919172420839 22-23 21.167574874329404 24.701332791061155 34.294294648304074 19.836797686305367 24-25 20.9138499956285 24.933518802857098 34.66089432068349 19.49173688083092 26-27 20.528372023572704 24.864704501270683 34.93551299318144 19.671410481975173 28-29 20.059933769304596 25.268061421061986 34.980799778379634 19.691205031253777 30-31 20.129484840715275 25.112799460095076 34.79596878846552 19.961746910724127 32-33 19.756186456746132 25.430072825763407 34.5627163135408 20.251024403949668 34-35 20.589350567954646 25.028758751649143 34.50465690391778 19.877233776478437 36-37 20.927893083830835 24.725346515783674 34.08272170968417 20.264038690701323 38-39 20.594859009062773 25.129241897116326 34.28936726605436 19.986531827766537 40-41 20.40584961008494 25.43083207422238 33.984747237978844 20.178571077713833 42-43 20.273640578032808 25.830454254952812 32.97107625378071 20.92482891323367 44-45 20.560076979639593 25.71341981262983 32.56766295178015 21.158840255950427 46-47 21.250648370820954 26.245706999261248 31.652382861005012 20.851261768912778 48-49 21.51921430350391 26.648513997908335 30.51259593151633 21.319675767071423 50-51 21.32530019878177 27.002786481268 30.27077446300189 21.40113885694835 52-53 21.58104785926375 27.407318265209135 29.452149332102078 21.559484543425032 54-55 21.01241248676126 27.035917024775852 29.767911845210378 22.183758643252517 56-57 21.634707484824343 27.35641454623332 28.90752942540276 22.101348543539576 58-59 22.11054733357238 26.69875777922952 29.199998035238007 21.990696851960095 60-61 21.732422164739308 27.110769663037253 29.88268835342233 21.274119818801104 62-63 21.959374660763732 27.20973286970429 29.863696401897418 20.967196067634564 64-65 21.62633778011402 27.390859004055407 29.78558673187411 21.197216483956463 66-67 21.722551541074694 27.58982480062401 29.09420724099787 21.59341641730342 68-69 21.85148928708433 27.360974740005318 29.444124903544218 21.343411069366137 70-71 21.956239200033796 27.459494293884823 29.18483647905629 21.399430027025087 72-73 21.815039156898727 27.4699495852473 29.16525861628597 21.549752641568006 74-75 21.356010126164467 28.46348126689045 28.73986818696664 21.440640419978447 76-77 21.273546213204053 27.98112864643964 29.155602709380172 21.58972243097613 78-79 21.076136037632782 27.776724344589894 29.31877041095029 21.828369206827034 80-81 21.178754631298986 28.40520218549169 28.765291515155685 21.65075166805364 82-83 21.331221655440192 27.915756234820677 28.68282781441338 22.070194295325752 84-85 20.881399392849776 27.82978179924745 28.520341497440732 22.768477310462043 86-87 21.10834493457294 27.941663367231552 28.258409138469187 22.691582559726324 88-89 21.08393364076673 27.646650741446415 28.366282686088752 22.9031329316981 90-91 20.865425478342253 27.40949634349752 28.700486141646792 23.02459203651344 92-93 20.984430635354055 27.665155263852032 28.763365992798118 22.5870481079958 94-95 20.675106521566132 27.222707440741388 28.897972135903537 23.204213901788933 96-97 20.46682851155429 27.23292400527781 29.131146429669684 23.169101053498217 98-99 20.61414626094953 27.32533806486601 28.47417895725508 23.586336716929377 100-101 20.387449070921964 27.23962677717552 28.941323610754964 23.431600541147553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1584.0 1 1207.5 2 1816.0 3 2403.0 4 1603.0 5 1277.0 6 1804.5 7 3313.5 8 4793.5 9 5253.5 10 4555.5 11 3562.0 12 3339.0 13 3347.0 14 3406.0 15 3543.5 16 3593.0 17 3576.5 18 3685.0 19 3865.5 20 3999.0 21 4271.5 22 4626.5 23 5054.0 24 5502.5 25 6163.0 26 7049.0 27 7893.0 28 8833.0 29 10333.0 30 12467.5 31 14594.0 32 17548.5 33 20269.0 34 22006.0 35 23697.0 36 26439.0 37 28871.0 38 28526.0 39 27546.5 40 27243.0 41 27631.5 42 27726.5 43 28756.0 44 30449.0 45 31212.5 46 30956.0 47 33653.5 48 43979.5 49 43818.5 50 34722.5 51 30230.5 52 27021.5 53 26526.5 54 26185.5 55 24837.5 56 23251.0 57 21552.5 58 20823.5 59 20143.0 60 18502.0 61 16729.0 62 14469.0 63 10654.5 64 7170.5 65 5491.5 66 4126.0 67 3109.0 68 2292.5 69 1545.5 70 1225.0 71 1031.5 72 939.5 73 968.0 74 777.0 75 570.5 76 424.0 77 257.5 78 160.5 79 128.0 80 96.0 81 59.0 82 35.5 83 22.0 84 13.0 85 7.5 86 3.5 87 1.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0103570190096438 2 0.01149360925854432 3 0.005304742734712763 4 0.012377733047663113 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004420618945593969 10-11 9.823597656875487E-5 12-13 2.947079297062646E-4 14-15 9.823597656875487E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0017682475782375877 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004715326875300234 34-35 5.894158594125292E-4 36-37 0.003732967109612685 38-39 1.473539648531323E-4 40-41 0.004224146992456459 42-43 0.012328615059378737 44-45 0.002505017402503249 46-47 0.002062955507943852 48-49 0.010658603457709904 50-51 6.385338476969066E-4 52-53 0.0024067814259344945 54-55 0.010904193399131791 56-57 0.004322382969025214 58-59 0.002161191484512607 60-61 0.006876518359812841 62-63 0.004469736933878346 64-65 0.0176333577940915 66-67 0.003045315273631401 68-69 3.438259179906421E-4 70-71 0.0013753036719625683 72-73 9.823597656875487E-5 74-75 0.0 76-77 0.0011788317188250585 78-79 0.011886553164819338 80-81 0.01512834039158825 82-83 0.012279497071094358 84-85 0.008546529961481673 86-87 0.010413013516288016 88-89 0.027899017345526383 90-91 0.024509876153904337 92-93 0.002799725332209514 94-95 0.008644765938050428 96-97 0.010805957422563036 98-99 0.010314777539719261 100-101 0.011788317188250583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1017957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.08460486364626 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01379168200066 35.03065553852522 2 12.858918499195395 11.85196356018502 3 4.361162042937713 6.029472884853504 4 2.0964533487958894 3.864568967773061 5 1.1097494109218593 2.5571181549999045 6 0.6814412114205346 1.8842369379671857 7 0.47303531304696067 1.5259751841824276 8 0.33023047614462847 1.2174832805647173 9 0.24590432136280782 1.0199163135841276 >10 1.5674904900319648 14.125362740531358 >50 0.16125700059382214 5.122324502675631 >100 0.09113142116384375 7.910955963509793 >500 0.005146244936713295 1.6078640471261632 >1k 0.003645256830171917 3.219483169772626 >5k 2.1442687236305396E-4 0.7166265177161167 >10k+ 4.288537447261079E-4 2.315992236033181 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12703 1.2478916103528932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10734 1.0544649724890147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7252 0.7124073020766103 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4476 0.4397042311217468 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3636 0.3571860108039927 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2862 0.28115136493977644 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2701 0.2653353727122069 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2140 0.21022498985713542 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2028 0.19922256048143486 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.171520015089046 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1683 0.16533114856521444 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1525 0.14980986426735118 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1476 0.1449963014154822 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1465 0.14391570567322587 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1230 0.12083025117956848 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1201 0.1179814078590746 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1124 0.11041723766328046 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1121 0.11012252973357421 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1092 0.10727368641308031 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1074 0.10550543883484273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.9647195313750974E-4 0.0 8 0.0 0.0 0.0 1.9647195313750974E-4 0.0 9 0.0 0.0 0.0 1.9647195313750974E-4 0.0 10-11 0.0 0.0 0.0 1.9647195313750974E-4 0.0 12-13 0.0 0.0 0.0 1.9647195313750974E-4 0.0 14-15 0.0 0.0 0.0 2.947079297062646E-4 0.0 16-17 0.0 0.0 0.0 3.929439062750195E-4 0.0 18-19 0.0 0.0 0.0 5.402978711281517E-4 0.0 20-21 0.0 0.0 0.0 6.87651835981284E-4 0.0 22-23 0.0 0.0 0.0 7.85887812550039E-4 0.0 24-25 0.0 0.0 0.0 0.0010314777539719262 0.0 26-27 0.0 0.0 0.0 0.0012770676953938133 0.0 28-29 0.0 0.0 0.0 0.0027014893556407587 0.0 30-31 0.0 0.0 0.0 0.004911798828437744 0.0 32-33 0.0 0.0 0.0 0.006974754336381596 0.0 34-35 0.0 0.0 0.0 0.00898859185604107 0.0 36-37 0.0 0.0 0.0 0.011935671153103716 0.0 38-39 0.0 0.0 0.0 0.015373930333010138 0.0 40-41 0.0 0.0 0.0 0.019843667266888486 0.0 42-43 0.0 0.0 0.0 0.027260483497829474 0.0 44-45 0.0 0.0 0.0 0.0359052494358799 0.0 46-47 0.0 0.0 0.0 0.05982570973037171 0.0 48-49 0.0 0.0 0.0 0.10462131504572394 0.0 50-51 0.0 0.0 0.0 0.13895478885650375 0.0 52-53 0.0 0.0 0.0 0.17382856053841172 0.0 54-55 0.0 0.0 0.0 0.21567708655670131 0.0 56-57 0.0 0.0 0.0 0.24878261066037172 0.0 58-59 0.0 0.0 0.0 0.2938238059171458 0.0 60-61 0.0 0.0 0.0 0.3724125871721497 0.0 62-63 0.0 0.0 0.0 0.4382306914732155 0.0 64-65 0.0 0.0 0.0 0.5083220607550221 0.0 66-67 0.0 0.0 0.0 0.6282190701571874 0.0 68-69 0.0 0.0 0.0 0.7685982806739381 0.0 70-71 0.0 0.0 0.0 0.8890847059355159 0.0 72-73 0.0 0.0 0.0 1.0173808913343099 0.0 74-75 0.0 0.0 0.0 1.1345764113808343 0.0 76-77 0.0 0.0 0.0 1.226672639414042 0.0 78-79 0.0 0.0 0.0 1.3412649060814945 0.0 80-81 0.0 0.0 0.0 1.5393577528323887 0.0 82-83 0.0 0.0 0.0 1.7539051256585494 0.0 84-85 0.0 0.0 0.0 1.9939938523925864 0.0 86-87 0.0 0.0 0.0 2.367585271283561 0.0 88-89 0.0 0.0 0.0 2.8123486551985986 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 80 2.2609674E-7 41.56551 2 ATCACGC 95 1.9283107E-8 39.998966 3 TACCTGG 910 0.0 37.06312 2 TAACGCA 130 2.055458E-10 36.53572 4 TCACGCA 125 2.1549204E-7 30.39772 4 GTACCTG 1155 0.0 29.502754 1 TATCACG 130 1.0013973E-5 25.578775 2 ATAACGC 170 1.122844E-7 25.146408 3 GTACATG 10695 0.0 25.130054 1 ACCTGGG 1310 0.0 24.655853 3 TACATGG 10950 0.0 24.597672 2 GTATCAA 17770 0.0 24.55063 1 ACATGGG 10870 0.0 23.771233 3 GGTATCA 13280 0.0 23.70784 1 GAGTACT 10815 0.0 23.536144 12-13 TAAGGTG 325 0.0 23.380564 5 CATGGGA 8500 0.0 22.071875 4 ATGGGAT 2890 0.0 22.020407 5 ATGGGAG 2755 0.0 21.89276 5 AGTACTT 11070 0.0 21.53541 12-13 >>END_MODULE