FastQCFastQC Report
Thu 26 May 2016
SRR936489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012873
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106591.0523530590705843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.8192537465210348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53390.5271144556128952No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40650.4013336321532907No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24390.24080017929197442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23100.22806413044873347No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.22006707652390775No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.2049615302214592No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17570.17346695982615787No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17440.17218348203575373No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15800.15599191606450166No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14270.1408863697620531No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13660.13486389705323373No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12720.12558336533800388No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11250.11107019340035719No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.10583755317794039No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10210.10080237107712418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG554.9543265E-543.1944582
TACCTGG9250.037.497462
TATCACG600.00394507531.6759382
GTACCTG12000.029.300241
ACGCATA700.00838317427.148127
ATGGGAG27800.026.658535
GTACATG120000.025.73671
GTATCAA163500.025.7185341
GTACACG2059.531504E-1025.4952661
TACATGG121400.025.2442072
ATGGGAT31450.025.0751655
ATATAGG4250.024.5954342
GAGTACT79450.024.28083212-13
ACATGGG121100.023.9727463
ACCTGGG13500.023.932933
GTATAGG3600.023.7569521
CATGGGA92500.023.577174
ATCACGC1050.002192309622.625673
AGTACTT82100.022.54217312-13
GTACTTT87900.021.9742414-15
Produced by FastQC (version 0.11.5)