##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936489_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012873 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.472046347370302 31.0 31.0 34.0 30.0 34.0 2 31.43949833789626 31.0 31.0 34.0 28.0 34.0 3 31.466552075136764 31.0 31.0 34.0 28.0 34.0 4 35.14101669212231 37.0 35.0 37.0 32.0 37.0 5 35.14212936863753 37.0 35.0 37.0 32.0 37.0 6 35.32411467182954 37.0 35.0 37.0 33.0 37.0 7 35.19955710143325 37.0 35.0 37.0 32.0 37.0 8 35.140468745834866 37.0 35.0 37.0 32.0 37.0 9 36.59415642434935 39.0 37.0 39.0 32.0 39.0 10-11 36.50590054231873 39.0 37.0 39.0 32.0 39.0 12-13 36.408945642740996 39.0 37.0 39.0 32.0 39.0 14-15 37.35978400056078 39.5 37.0 41.0 32.0 41.0 16-17 37.28950174404886 39.0 36.5 41.0 32.0 41.0 18-19 37.355835331774074 39.5 37.0 41.0 32.0 41.0 20-21 37.33039927019478 39.5 37.0 41.0 31.5 41.0 22-23 37.196106027112975 39.0 36.0 41.0 31.0 41.0 24-25 37.27875360484484 39.0 36.0 41.0 31.0 41.0 26-27 37.240716753235596 39.0 36.0 41.0 31.0 41.0 28-29 37.12538738815232 39.0 36.0 41.0 31.0 41.0 30-31 36.85965960194417 39.0 36.0 41.0 31.0 41.0 32-33 36.57187327532672 39.0 35.0 41.0 30.0 41.0 34-35 36.297228773992394 38.5 35.0 40.0 30.0 41.0 36-37 36.0543409687098 38.0 35.0 40.0 30.0 41.0 38-39 35.80223285643906 38.0 35.0 40.0 29.5 41.0 40-41 35.55735566058134 38.0 35.0 40.0 28.5 41.0 42-43 35.21733425612095 38.0 35.0 40.0 27.0 41.0 44-45 34.772251802545824 38.0 34.0 40.0 25.0 41.0 46-47 34.19630545981579 38.0 33.5 40.0 22.5 41.0 48-49 33.852903078668305 38.0 33.0 40.0 19.5 41.0 50-51 32.85768995718121 37.0 32.0 39.5 16.5 40.5 52-53 32.953631402949824 37.5 33.0 39.5 15.0 40.5 54-55 33.34368869542381 38.0 33.0 40.0 14.5 41.0 56-57 33.24377932870162 37.5 33.0 40.0 13.5 41.0 58-59 32.9860352679951 37.0 33.0 40.0 9.5 41.0 60-61 32.666954790975765 36.5 32.5 40.0 8.0 41.0 62-63 32.34044001567817 36.0 32.0 39.5 7.0 41.0 64-65 31.984023663381294 35.5 31.5 39.0 4.0 41.0 66-67 31.594687093051153 35.0 31.0 39.0 2.0 40.5 68-69 31.203274744217687 35.0 31.0 38.5 2.0 40.0 70-71 30.767421483246174 35.0 30.5 37.5 2.0 40.0 72-73 30.395168989596918 35.0 30.0 37.0 2.0 39.5 74-75 29.989734645903287 35.0 30.0 36.5 2.0 39.0 76-77 29.584571313481554 34.5 29.0 36.0 2.0 39.0 78-79 29.158294771407668 34.0 29.0 35.5 2.0 37.5 80-81 28.703377422440916 34.0 29.0 35.0 2.0 37.0 82-83 28.235203722480506 34.0 27.5 35.0 2.0 37.0 84-85 27.91244460065576 34.0 27.0 35.0 2.0 36.0 86-87 27.593550227916037 34.0 26.5 35.0 2.0 36.0 88-89 27.18944379009017 33.5 26.0 35.0 2.0 35.5 90-91 26.762083696574003 33.0 25.0 35.0 2.0 35.0 92-93 26.421501511048277 33.0 24.5 35.0 2.0 35.0 94-95 25.98827444309405 33.0 23.5 35.0 2.0 35.0 96-97 25.43256163408443 33.0 19.5 35.0 2.0 35.0 98-99 24.77996352948494 32.5 9.5 35.0 2.0 35.0 100-101 22.50201604742154 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5449244500586055 1101 2 0.6918031355333696 1101 3 0.7382343157331022 1101 4 0.7011428196359475 1101 5 0.6147598243655992 1101 6 0.46178425235323317 1101 7 0.6433876366588294 1101 8 0.6481889483107679 1101 9 0.7348407317417625 1101 10-11 0.7132001671721326 1101 12-13 0.8629878933739192 1101 14-15 1.0201222891168413 1101 16-17 1.0560607806511584 1101 18-19 1.0153829880012424 1101 20-21 1.048290453919705 1101 22-23 0.9671648284857355 1101 24-25 0.9650430977844451 1101 26-27 0.8567635849299222 1101 28-29 0.9545288441439368 1101 30-31 1.0231777181269663 1101 32-33 0.9621023808235591 1101 34-35 0.9717611181128021 1101 36-37 0.9134451144755857 1101 38-39 0.6986619617298189 1101 40-41 0.7560243618817637 1101 42-43 0.8162524274256597 1101 44-45 0.694564244470314 1101 46-47 0.9558456361015857 1101 48-49 1.2230089955974321 1101 50-51 1.2626312289286687 1101 52-53 1.4058010836451658 1101 54-55 1.3408673773601194 1101 56-57 1.3409804381622976 1101 58-59 1.2998967475922711 1101 60-61 1.278897786607864 1101 62-63 1.325255979862547 1101 64-65 1.4881084101400788 1101 66-67 1.3731180688721096 1101 68-69 1.2419220802948026 1101 70-71 1.2177922568201893 1101 72-73 1.068956866532833 1101 74-75 1.0859449566476904 1101 76-77 1.1945920877558542 1101 78-79 1.1835739335524735 1101 80-81 1.2633122593154518 1101 82-83 1.342160544522443 1101 84-85 1.4226764817315818 1101 86-87 1.3241794645734544 1101 88-89 1.3494444887598114 1101 90-91 0.8903879717781571 1101 92-93 0.9510297793930533 1101 94-95 1.2550666880101922 1101 96-97 1.2725917521832173 1101 98-99 1.0139337456129134 1101 100-101 1.2502566317889006 1102 1 0.4770160684829676 1102 2 0.5505065262780384 1102 3 0.4538863958452879 1102 4 0.5801259558050873 1102 5 0.49851804952830037 1102 6 0.4888742527128045 1102 7 0.6091350028089124 1102 8 0.5582748855298547 1102 9 0.807826241072604 1102 10-11 0.7389381845034109 1102 12-13 0.5921058682759366 1102 14-15 0.7744101608222778 1102 16-17 0.8715977983779553 1102 18-19 0.822840468854146 1102 20-21 0.818050620760161 1102 22-23 0.7546661768730729 1102 24-25 0.508234640699122 1102 26-27 0.5746593815571615 1102 28-29 0.7145734308182696 1102 30-31 0.6508610089111855 1102 32-33 0.6182743990897066 1102 34-35 0.4598809268215902 1102 36-37 0.7180476098910731 1102 38-39 0.3913734787554617 1102 40-41 0.5833732526084532 1102 42-43 0.5436912106409224 1102 44-45 0.4376443206991425 1102 46-47 0.361623925267736 1102 48-49 0.5108555311409191 1102 50-51 0.6726596349550604 1102 52-53 0.6193712537217593 1102 54-55 0.8927641088692297 1102 56-57 0.9791236189181163 1102 58-59 0.9263898618276443 1102 60-61 0.6315639978192635 1102 62-63 0.6096254739476166 1102 64-65 0.6236626872246873 1102 66-67 0.7975975913632141 1102 68-69 0.4282008908373989 1102 70-71 0.2473848640619245 1102 72-73 0.47770879922134313 1102 74-75 0.3831510981025126 1102 76-77 0.4686922639451332 1102 78-79 0.20636707295774315 1102 80-81 0.5221091382482506 1102 82-83 0.4264509332175628 1102 84-85 0.3119325314960619 1102 86-87 0.12375517202734088 1102 88-89 0.1805365027111243 1102 90-91 0.22744165557233487 1102 92-93 -0.028399223519461003 1102 94-95 0.09390024307833045 1102 96-97 -0.10064545611565734 1102 98-99 0.18926724674556183 1102 100-101 0.44260498316065977 1103 1 0.3376864326016076 1103 2 0.36656632854473514 1103 3 0.3281845115227142 1103 4 0.3094104643999458 1103 5 0.48639913396110757 1103 6 0.4401781212580502 1103 7 0.2739322745027337 1103 8 0.3083317252418709 1103 9 0.528856934517087 1103 10-11 0.5022541788538888 1103 12-13 0.491775164496957 1103 14-15 0.44477427946226555 1103 16-17 0.7423270691072261 1103 18-19 0.6217036746139044 1103 20-21 0.5540146567241919 1103 22-23 0.4971202744995793 1103 24-25 0.6117759269990302 1103 26-27 0.46340511857875555 1103 28-29 0.2488184960978188 1103 30-31 0.4397100908637199 1103 32-33 0.48708834376572696 1103 34-35 0.37381526834213474 1103 36-37 0.34901319602906966 1103 38-39 0.304133132894421 1103 40-41 0.07727245685249073 1103 42-43 0.09573571785784907 1103 44-45 0.2469315851588263 1103 46-47 0.497577626738682 1103 48-49 0.5409392405349749 1103 50-51 0.6569959882569307 1103 52-53 0.7300571195800316 1103 54-55 0.762577054544245 1103 56-57 0.5927651498010249 1103 58-59 0.5558087188327079 1103 60-61 0.2818619757034426 1103 62-63 0.3364710421724979 1103 64-65 0.6370251867940837 1103 66-67 0.40172449893839257 1103 68-69 0.6495691777229275 1103 70-71 0.6880993220177629 1103 72-73 0.5249133188396549 1103 74-75 0.35892015663019095 1103 76-77 0.5736218171505456 1103 78-79 0.7207630218364542 1103 80-81 0.7850496460163434 1103 82-83 0.5998189445166098 1103 84-85 0.5983220040924344 1103 86-87 0.8086220293079869 1103 88-89 0.7241136152537564 1103 90-91 0.8442662103279268 1103 92-93 0.6870784023029906 1103 94-95 0.6557280704233968 1103 96-97 0.6194930260917886 1103 98-99 0.8807895175781759 1103 100-101 1.131051019882559 1104 1 0.46840834227088735 1104 2 0.5411917031701101 1104 3 0.5311815145197158 1104 4 0.5892569337200939 1104 5 0.698513335548995 1104 6 0.5968950885013342 1104 7 0.6325210541441493 1104 8 0.6596961502905074 1104 9 0.6649313863810136 1104 10-11 0.8239430163321941 1104 12-13 0.7417252144470083 1104 14-15 0.8865062317205314 1104 16-17 0.7519122207976423 1104 18-19 0.9346065612010008 1104 20-21 1.0819603951962478 1104 22-23 0.7289726326677268 1104 24-25 0.7944721781163366 1104 26-27 0.5029550423392024 1104 28-29 0.6272611061193771 1104 30-31 0.7979465912054806 1104 32-33 0.7242564388285544 1104 34-35 0.6543820699615708 1104 36-37 0.8220874901560009 1104 38-39 0.6930021586055588 1104 40-41 0.7215282011082351 1104 42-43 0.6324200861211651 1104 44-45 0.4226821503830749 1104 46-47 0.6761359105601201 1104 48-49 0.6357076300402085 1104 50-51 0.5618858352520419 1104 52-53 0.6430283588670633 1104 54-55 0.6651166202417045 1104 56-57 0.7112046050825853 1104 58-59 0.8843223105041886 1104 60-61 1.012873069872434 1104 62-63 1.15452141285445 1104 64-65 0.8987634485323426 1104 66-67 0.8260370285140795 1104 68-69 0.9540594243184337 1104 70-71 0.9546538201512078 1104 72-73 0.9864885856780781 1104 74-75 0.7330933518560165 1104 76-77 1.0129824565111853 1104 78-79 0.9157785326414363 1104 80-81 1.0422661137591298 1104 82-83 0.9929416113234844 1104 84-85 0.8445862661461696 1104 86-87 0.7758022175408108 1104 88-89 0.7551352252227339 1104 90-91 1.0609863323112059 1104 92-93 1.140241029149827 1104 94-95 0.9306952085484319 1104 96-97 1.0749691289363135 1104 98-99 1.1089245404500403 1104 100-101 1.4409621613726493 1105 1 0.28057231384365267 1105 2 0.31573359962981584 1105 3 0.226306937042402 1105 4 0.4597669572221861 1105 5 0.43351366326741925 1105 6 0.3943267123929459 1105 7 0.45479935263008997 1105 8 0.530972098567176 1105 9 0.5290827361127555 1105 10-11 0.6031361735714995 1105 12-13 0.5509762373966609 1105 14-15 0.371262175128237 1105 16-17 0.48236100777130275 1105 18-19 0.6821056014541895 1105 20-21 0.6026885855351125 1105 22-23 0.5048146349336662 1105 24-25 0.6206882475551794 1105 26-27 0.4398088518318062 1105 28-29 0.3374798347591508 1105 30-31 0.3587191639823786 1105 32-33 0.25786209056686715 1105 34-35 0.013281966028010572 1105 36-37 0.3618359896737857 1105 38-39 0.427003413298948 1105 40-41 0.1643942391797495 1105 42-43 0.323487418212288 1105 44-45 0.11812545977735311 1105 46-47 0.4873139178448369 1105 48-49 0.43716972238189555 1105 50-51 0.6164851292529221 1105 52-53 0.3134531755788359 1105 54-55 0.4804927005420794 1105 56-57 0.640936156876144 1105 58-59 0.5139396290184095 1105 60-61 0.1836930213701109 1105 62-63 0.4943747822680251 1105 64-65 0.47352772535141696 1105 66-67 0.5653417036514838 1105 68-69 0.5240474391874876 1105 70-71 0.6276905527048839 1105 72-73 0.6304721435765579 1105 74-75 0.7859178165045613 1105 76-77 0.4853094445915964 1105 78-79 0.5038669042554034 1105 80-81 0.5423129306768892 1105 82-83 0.673006031402327 1105 84-85 0.7421302506404075 1105 86-87 0.88473769446475 1105 88-89 0.7291565175569339 1105 90-91 0.9654840336005428 1105 92-93 0.9152440722494859 1105 94-95 0.9647957151074813 1105 96-97 1.2088659819031022 1105 98-99 1.2635266434659833 1105 100-101 1.6609834162533126 1106 1 0.19592828607701662 1106 2 0.2514612239258085 1106 3 0.2527518412378029 1106 4 0.42920201698463245 1106 5 0.39123595586180215 1106 6 0.18064356620395472 1106 7 0.34427661081466 1106 8 0.4202226941395111 1106 9 0.5876465719530515 1106 10-11 0.34790614366695394 1106 12-13 0.49137423082624565 1106 14-15 0.5747524995262765 1106 16-17 0.6701542466960859 1106 18-19 0.5268776236391375 1106 20-21 0.3320231115677359 1106 22-23 0.2944535418042449 1106 24-25 0.33657972186955476 1106 26-27 0.19500716762599524 1106 28-29 0.32743625584046043 1106 30-31 0.28876856337308965 1106 32-33 0.22741141440338453 1106 34-35 0.4576446896358064 1106 36-37 0.4106607953315873 1106 38-39 0.3921318834289238 1106 40-41 0.4071942033614633 1106 42-43 0.16385444374588332 1106 44-45 0.38185766956060263 1106 46-47 0.3533814987484618 1106 48-49 0.30243465646319123 1106 50-51 0.17695600917024734 1106 52-53 0.29606451542537116 1106 54-55 0.40282717620522845 1106 56-57 0.3171546782896897 1106 58-59 0.5248754004827987 1106 60-61 0.5178338208268585 1106 62-63 0.53006248409568 1106 64-65 0.5759451800515407 1106 66-67 0.5865220655281433 1106 68-69 0.4886633533280609 1106 70-71 0.6715115246863981 1106 72-73 0.6941354218081806 1106 74-75 0.4241885393437599 1106 76-77 0.5130084552979106 1106 78-79 0.7203292325561392 1106 80-81 0.6617715894685077 1106 82-83 0.7354249971865308 1106 84-85 0.8930315843120198 1106 86-87 1.0645315154367303 1106 88-89 1.0197641039452385 1106 90-91 1.1329974981059259 1106 92-93 1.2685117449374772 1106 94-95 0.7944825475954183 1106 96-97 0.5785596982185197 1106 98-99 1.0882003145981223 1106 100-101 1.3066542536364132 1107 1 0.3010604080237762 1107 2 0.2498276455409929 1107 3 0.02643806519795433 1107 4 0.28283463059518965 1107 5 0.31080364511260683 1107 6 0.31607331040625297 1107 7 0.40394683825223865 1107 8 0.38048848414561576 1107 9 0.4961546969955748 1107 10-11 0.44712858155961044 1107 12-13 0.37401942625928797 1107 14-15 0.41731980622465414 1107 16-17 0.351777859280908 1107 18-19 0.5960847152599982 1107 20-21 0.6743461324773605 1107 22-23 0.5909842418575195 1107 24-25 0.5926203837470965 1107 26-27 0.5165628042665631 1107 28-29 0.4431663169516611 1107 30-31 0.549720562319969 1107 32-33 0.3867970687756568 1107 34-35 0.5747167764725631 1107 36-37 0.6529622590142807 1107 38-39 0.5391932534967125 1107 40-41 0.3948283948903537 1107 42-43 0.5740646178734963 1107 44-45 0.6043211835122761 1107 46-47 0.8088145825539499 1107 48-49 0.5853105318942227 1107 50-51 0.5607488618893228 1107 52-53 0.7922840492768373 1107 54-55 0.8882826742257706 1107 56-57 0.6627373462792434 1107 58-59 0.6259734938167583 1107 60-61 0.6845436313971476 1107 62-63 0.5521228602339505 1107 64-65 0.6722489991263316 1107 66-67 0.3673853441173094 1107 68-69 0.3984949507450821 1107 70-71 0.4786220522995279 1107 72-73 0.6183078760896308 1107 74-75 0.6447247135190857 1107 76-77 0.44948089782890577 1107 78-79 0.657230169146974 1107 80-81 0.6927022343918239 1107 82-83 0.8519342990416021 1107 84-85 0.5211040413323005 1107 86-87 0.544759385927275 1107 88-89 0.45746460161678826 1107 90-91 0.4299275369531088 1107 92-93 0.20115468746361387 1107 94-95 0.3365893777907303 1107 96-97 0.19222150243472313 1107 98-99 0.4070825017024866 1107 100-101 0.8570358783975358 1108 1 0.25683713484054493 1108 2 0.29742499194016503 1108 3 0.295168813984958 1108 4 0.416325608079795 1108 5 0.5249469831707216 1108 6 0.48548483660152897 1108 7 0.1902319674568389 1108 8 0.2189955099865344 1108 9 0.46684174382542665 1108 10-11 0.03750110104933668 1108 12-13 0.4883746128427262 1108 14-15 0.37771128730735626 1108 16-17 0.522280571487201 1108 18-19 0.5035566561911438 1108 20-21 0.3755217342055346 1108 22-23 0.6113594360733003 1108 24-25 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2316 80-81 -2.4450270481228493 2316 82-83 -2.175686279426113 2316 84-85 -1.8657332115252814 2316 86-87 -2.567439409253449 2316 88-89 -3.176670496467853 2316 90-91 -3.1514812713570564 2316 92-93 -3.5113887555391656 2316 94-95 -3.842116793046465 2316 96-97 -4.912570743471981 2316 98-99 -6.028087022548366 2316 100-101 -6.2693074757884375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1715.0 3 655.0 4 663.0 5 720.0 6 842.0 7 1034.0 8 1359.0 9 1816.0 10 2475.0 11 3769.0 12 5292.0 13 7141.0 14 8150.0 15 10499.0 16 13915.0 17 18472.0 18 14315.0 19 7823.0 20 7375.0 21 7653.0 22 8304.0 23 9478.0 24 10736.0 25 12167.0 26 13981.0 27 16489.0 28 19797.0 29 23733.0 30 28541.0 31 34896.0 32 43760.0 33 55538.0 34 73685.0 35 101314.0 36 134109.0 37 164597.0 38 127080.0 39 18978.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.83360575189993 18.518668490471935 14.021935044220694 19.625790713407437 2 15.794811530371408 21.690252115777728 39.95398121722643 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46-47 21.467344085681788 27.117855901680777 30.11480317199545 21.29999684064198 48-49 21.572238869334704 27.46745585498418 29.194257815778172 21.76604745990295 50-51 21.45850415627331 27.89913576962018 29.05772873325359 21.584631340852916 52-53 21.70805733248159 28.3022109359033 28.346344743288356 21.643386988326757 54-55 21.05232237530343 27.90888719609277 28.691429651896723 22.34736077670708 56-57 21.853130452728944 28.172692091586594 27.8238655608232 22.150311894861265 58-59 22.125509940601763 27.5274860638041 28.159632205880975 22.187371789713158 60-61 21.790197361112824 27.99096508226465 28.789098853355387 21.429738703267134 62-63 21.976099873814917 28.118736411406815 28.661737088738327 21.24342662603994 64-65 21.600991240259756 28.365663002993962 28.618063971526524 21.415281785219758 66-67 21.726747489727618 28.37147143752126 28.072692373580466 21.829088699170658 68-69 21.68151576868481 28.36255014503487 28.40901067703512 21.546923409245203 70-71 21.826956589565185 28.293745884020964 28.1788193570236 21.700478169390248 72-73 21.711146632334604 28.24639903322595 28.167858514512968 21.874595819926483 74-75 21.177901620536637 29.346932670249647 27.903521126412915 21.571644582800797 76-77 21.243192834994467 28.74883430924708 28.189648320541384 21.81832453521707 78-79 21.17712164916696 28.69084685284202 28.267378470053856 21.864653027937166 80-81 21.136551688728186 29.406066901401495 27.713734752274444 21.743646657595868 82-83 21.33631648530459 28.906821590463654 27.66057233468832 22.09628958954343 84-85 21.066716172366736 28.80027017472484 27.63593165843437 22.49708199447406 86-87 21.282847481055462 28.984474119418458 27.4155456273295 22.31713277219658 88-89 21.219825622124002 28.513216571279337 27.467551605671602 22.79940620092506 90-91 21.088601608461033 28.28343115315825 27.850437116144196 22.777530122236524 92-93 21.241101731317464 28.716585513732145 27.807231580620854 22.235081174329537 94-95 20.78478749103304 28.339597658049332 28.078475786858593 22.797139064059035 96-97 20.670092913494674 28.410201723984713 28.20951449983708 22.710190862683532 98-99 20.85373392558417 28.608065697971824 27.601532241658884 22.936668134785123 100-101 20.822697389051605 28.505762792886618 27.932619348504893 22.738920469556877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 859.0 1 670.5 2 1107.0 3 1593.5 4 1241.5 5 1115.5 6 1636.0 7 2947.0 8 4335.5 9 4743.0 10 4009.5 11 3251.0 12 3155.0 13 3221.0 14 3291.5 15 3300.0 16 3295.0 17 3323.5 18 3320.5 19 3342.0 20 3461.5 21 3800.0 22 4183.5 23 4603.0 24 5084.0 25 5636.5 26 6532.0 27 7612.0 28 8535.0 29 9980.5 30 12082.5 31 14386.5 32 17471.0 33 20108.5 34 22155.5 35 23739.0 36 26290.0 37 29002.0 38 28784.0 39 27750.0 40 27224.0 41 27564.5 42 27726.0 43 28552.5 44 30012.0 45 30822.5 46 30801.0 47 33708.0 48 44077.0 49 44192.5 50 35433.0 51 30946.5 52 27605.5 53 26886.5 54 26680.5 55 25524.0 56 23760.0 57 21952.5 58 21147.0 59 20316.5 60 18805.0 61 17456.5 62 15433.0 63 11254.5 64 7357.0 65 5537.0 66 4111.0 67 3095.5 68 2302.5 69 1610.0 70 1212.0 71 991.5 72 938.0 73 926.5 74 747.0 75 578.0 76 414.5 77 225.5 78 136.0 79 93.5 80 61.0 81 42.0 82 27.5 83 16.5 84 12.5 85 7.0 86 6.0 87 5.0 88 1.5 89 2.0 90 2.5 91 2.0 92 1.5 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033370422550507324 2 0.023991161774477156 3 0.021226748072068265 4 0.05015436288656129 5 0.05588064841297971 6 0.049759446643360024 7 0.050648008190562886 8 0.044428077360142884 9 0.04304587050893843 10-11 0.05272131846736955 12-13 0.04882152056575701 14-15 0.04205857990093526 16-17 0.050746737251363194 18-19 0.055781919352179396 20-21 0.012439861660840006 22-23 0.02260895492327271 24-25 0.007404679560023813 26-27 0.01224240353923937 28-29 0.007009763316822544 30-31 0.010761467627234608 32-33 0.005232640222416828 34-35 0.009280531715229846 36-37 0.010514644975233816 38-39 0.013279058677642704 40-41 0.001826487624805874 42-43 0.0023694974592076206 44-45 0.003208694476010319 46-47 8.885615472028576E-4 48-49 0.0017771230944057153 50-51 6.417388952020638E-4 52-53 0.00434407867521397 54-55 0.008342605637626831 56-57 0.018955979673660965 58-59 0.01308160055604207 60-61 0.013377787738443024 62-63 0.007404679560023813 64-65 4.936453040015876E-5 66-67 0.008342605637626831 68-69 0.0183142407784589 70-71 0.005133911161616511 72-73 0.0019745812160063504 74-75 0.002912507293609367 76-77 0.006071837239219527 78-79 0.0045909013272147645 80-81 0.009774177019231434 82-83 0.007305950499223496 84-85 0.019252166856061917 86-87 0.004738994918415241 88-89 0.00715785690802302 90-91 0.004097256023213177 92-93 0.01737631470085588 94-95 0.012587955252040484 96-97 0.010169093262432703 98-99 0.023596245531275886 100-101 0.03815878199932272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11076597349701 #Duplication Level Percentage of deduplicated Percentage of total 1 76.88021105733631 36.98765842172564 2 12.511480104134723 12.038737825441794 3 4.250179511994076 6.134381755406962 4 1.9246992496189406 3.7039502067112866 5 1.060807932139908 2.551814108300621 6 0.6503117942288683 1.8772199125170017 7 0.44450786718103774 1.4969929779927638 8 0.3037107927414396 1.1689407098566924 9 0.24493450234304134 1.0605587868954913 >10 1.4943509002573903 14.074649951990967 >50 0.1431174050953297 4.80259626871807 >100 0.0838416362498465 7.674136008608616 >500 0.004336636322684859 1.4827270821215104 >1k 0.002891090881789906 2.5317833177232947 >5k 4.130129831128437E-4 1.3548612449002302 >10k+ 2.0650649155642186E-4 1.0589914210890632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10659 1.0523530590705843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8298 0.8192537465210348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5339 0.5271144556128952 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4065 0.4013336321532907 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2439 0.24080017929197442 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2310 0.22806413044873347 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2229 0.22006707652390775 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2076 0.2049615302214592 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1757 0.17346695982615787 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1744 0.17218348203575373 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1580 0.15599191606450166 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1427 0.1408863697620531 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1366 0.13486389705323373 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1272 0.12558336533800388 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1125 0.11107019340035719 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1072 0.10583755317794039 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1021 0.10080237107712418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.872906080031751E-5 0.0 10-11 0.0 0.0 0.0 9.872906080031751E-5 0.0 12-13 0.0 0.0 0.0 3.4555171280111125E-4 0.0 14-15 0.0 0.0 0.0 4.936453040015876E-4 0.0 16-17 0.0 0.0 0.0 5.923743648019052E-4 0.0 18-19 0.0 0.0 0.0 7.404679560023813E-4 0.0 20-21 0.0 0.0 0.0 8.885615472028576E-4 0.0 22-23 0.0 0.0 0.0 0.0011353841992036515 0.0 24-25 0.0 0.0 0.0 0.001431571381604604 0.0 26-27 0.0 0.0 0.0 0.0017277585640055565 0.0 28-29 0.0 0.0 0.0 0.0028137782328090492 0.0 30-31 0.0 0.0 0.0 0.0056275564656180985 0.0 32-33 0.0 0.0 0.0 0.008490699228827305 0.0 34-35 0.0 0.0 0.0 0.011748758235237784 0.0 36-37 0.0 0.0 0.0 0.015796649728050803 0.0 38-39 0.0 0.0 0.0 0.019104073264861438 0.0 40-41 0.0 0.0 0.0 0.023892432713676838 0.0 42-43 0.0 0.0 0.0 0.031149018682500175 0.0 44-45 0.0 0.0 0.0 0.041861121779334626 0.0 46-47 0.0 0.0 0.0 0.0704925494114267 0.0 48-49 0.0 0.0 0.0 0.12336196146999673 0.0 50-51 0.0 0.0 0.0 0.16285358579012374 0.0 52-53 0.0 0.0 0.0 0.19908715110384026 0.0 54-55 0.0 0.0 0.0 0.243465863933583 0.0 56-57 0.0 0.0 0.0 0.2797487937776997 0.0 58-59 0.0 0.0 0.0 0.3242756001986429 0.0 60-61 0.0 0.0 0.0 0.4105647993381204 0.0 62-63 0.0 0.0 0.0 0.4769600927263339 0.0 64-65 0.0 0.0 0.0 0.5505132430225704 0.0 66-67 9.872906080031751E-5 0.0 0.0 0.6828595490253961 0.0 68-69 9.872906080031751E-5 0.0 0.0 0.8226598991186457 0.0 70-71 9.872906080031751E-5 0.0 0.0 0.946219318710243 0.0 72-73 9.872906080031751E-5 0.0 0.0 1.0793554571994712 0.0 74-75 9.872906080031751E-5 0.0 0.0 1.1920053155726333 0.0 76-77 9.872906080031751E-5 0.0 0.0 1.285797923332935 0.0 78-79 9.872906080031751E-5 0.0 0.0 1.4021007569557091 0.0 80-81 9.872906080031751E-5 0.0 0.0 1.60760529701157 0.0 82-83 9.872906080031751E-5 0.0 0.0 1.8346327723219003 0.0 84-85 9.872906080031751E-5 0.0 0.0 2.0816528824442946 0.0 86-87 9.872906080031751E-5 0.0 0.0 2.467239229399935 0.0 88-89 9.872906080031751E-5 0.0 0.0 2.9153210718421754 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 55 4.9543265E-5 43.194458 2 TACCTGG 925 0.0 37.49746 2 TATCACG 60 0.003945075 31.675938 2 GTACCTG 1200 0.0 29.30024 1 ACGCATA 70 0.008383174 27.14812 7 ATGGGAG 2780 0.0 26.65853 5 GTACATG 12000 0.0 25.7367 1 GTATCAA 16350 0.0 25.718534 1 GTACACG 205 9.531504E-10 25.495266 1 TACATGG 12140 0.0 25.244207 2 ATGGGAT 3145 0.0 25.075165 5 ATATAGG 425 0.0 24.595434 2 GAGTACT 7945 0.0 24.280832 12-13 ACATGGG 12110 0.0 23.972746 3 ACCTGGG 1350 0.0 23.93293 3 GTATAGG 360 0.0 23.756952 1 CATGGGA 9250 0.0 23.57717 4 ATCACGC 105 0.0021923096 22.62567 3 AGTACTT 8210 0.0 22.542173 12-13 GTACTTT 8790 0.0 21.97424 14-15 >>END_MODULE