FastQCFastQC Report
Thu 26 May 2016
SRR936489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012873
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127221.2560311115016394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110311.0890802696883024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74120.7317797986519534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44950.44378712829742717No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.3467364615307151No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28220.278613409578496No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.2741706018424817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.20851577641027058No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21080.2081208601670693No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17530.1730720435829566No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.16546990590133215No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16160.1595461622533131No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14900.1471063005924731No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14430.14246603473485817No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13960.13782576887724324No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12150.11995580887238577No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.11245240025156164No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11310.11166256776515911No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.11037908997475498No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11150.11008290279235403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC704.310119E-640.7136963
TACCTGG10400.038.3648262
TCACGCA905.6977296E-736.943914
TATCACG851.6321743E-533.5289232
GTACCTG13150.030.3522551
CGCGGAA1107.6397555E-1125.90807582-83
ACCTGGG15500.025.4351123
GTATCAA191600.024.2042541
GTACACG2002.0461812E-823.7578661
CACGCAG1601.8594274E-623.7496555
GAGTACT111200.023.62150812-13
GTACATG114200.023.5914361
CGTCACT2853.6379788E-1223.341061
TACATGG116100.023.238252
ATGGGAG27400.023.229595
GGTATCA143150.022.6707951
ACATGGG115400.022.2267133
AGTACTT112550.022.15649612-13
AATTCGC1306.2573235E-1021.92167778-79
GTACTTT119700.021.74571614-15
Produced by FastQC (version 0.11.5)