##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936489_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012873 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76591635871427 31.0 31.0 34.0 30.0 34.0 2 31.856307750330004 31.0 31.0 34.0 30.0 34.0 3 31.92587619573234 31.0 31.0 34.0 30.0 34.0 4 35.74861310351841 37.0 35.0 37.0 35.0 37.0 5 35.543229012916726 37.0 35.0 37.0 33.0 37.0 6 35.66826739383911 37.0 35.0 37.0 33.0 37.0 7 35.6126296189157 37.0 35.0 37.0 33.0 37.0 8 35.605547783384495 37.0 35.0 37.0 33.0 37.0 9 37.18196654467046 39.0 37.0 39.0 33.0 39.0 10-11 37.04103772141226 39.0 37.0 39.0 33.0 39.0 12-13 36.93665000449218 39.0 37.0 39.0 33.0 39.0 14-15 37.87155645377061 40.0 37.0 41.0 33.0 41.0 16-17 37.845733867918284 39.0 37.0 41.0 33.0 41.0 18-19 38.11036131874381 40.0 37.0 41.0 33.0 41.0 20-21 38.18584807769582 40.0 37.0 41.0 33.0 41.0 22-23 38.05830839601806 40.0 37.0 41.0 33.0 41.0 24-25 37.9142498615325 40.0 37.0 41.0 33.0 41.0 26-27 37.647983508297685 39.5 36.5 41.0 32.5 41.0 28-29 37.50461212807529 39.0 36.0 41.0 32.0 41.0 30-31 37.29502366041942 39.0 36.0 41.0 32.0 41.0 32-33 36.96144531446687 39.0 35.0 41.0 31.5 41.0 34-35 36.68314240778459 39.0 35.0 41.0 31.0 41.0 36-37 36.312205972515805 38.5 35.0 40.5 30.5 41.0 38-39 36.070936336539724 38.0 35.0 40.0 30.0 41.0 40-41 35.75890165894441 38.0 35.0 40.0 29.5 41.0 42-43 35.3464881579428 38.0 35.0 40.0 27.5 41.0 44-45 34.845606013784554 38.0 34.0 40.0 25.0 41.0 46-47 34.14565991985175 38.0 33.5 40.0 22.0 41.0 48-49 33.516283383997795 38.0 33.0 40.0 16.5 41.0 50-51 33.0928181519302 38.0 33.0 40.0 10.5 41.0 52-53 32.781287979835575 37.0 33.0 40.0 7.5 41.0 54-55 32.49059704424938 37.0 32.0 40.0 6.0 41.0 56-57 32.17729715373991 36.5 31.5 40.0 2.0 41.0 58-59 31.845858266534897 36.0 31.0 39.0 2.0 41.0 60-61 31.4677284318962 35.5 31.0 39.0 2.0 40.0 62-63 31.06495384910053 35.0 30.0 39.0 2.0 40.0 64-65 30.638450723832108 35.0 29.5 38.0 2.0 40.0 66-67 30.172646521330908 35.0 29.0 38.0 2.0 40.0 68-69 29.909935401575517 34.5 29.0 37.5 2.0 40.0 70-71 29.483413024140244 34.0 28.5 37.0 2.0 39.0 72-73 29.054101057092055 34.0 28.0 36.0 2.0 39.0 74-75 28.53197439363079 34.0 27.0 36.0 2.0 38.5 76-77 27.145063596324512 31.5 25.5 34.5 2.0 36.5 78-79 27.890169843603296 33.0 27.0 35.0 2.0 37.0 80-81 27.672666267143068 33.0 26.0 35.0 2.0 37.0 82-83 27.331349537404986 33.0 26.0 35.0 2.0 36.0 84-85 26.909498031836172 33.0 25.5 35.0 2.0 36.0 86-87 26.522948582892425 33.0 25.0 35.0 2.0 35.0 88-89 26.09325058521651 32.5 24.0 35.0 2.0 35.0 90-91 25.58724835196515 32.0 21.5 34.5 2.0 35.0 92-93 25.083227117318756 32.0 19.5 34.0 2.0 35.0 94-95 24.487163741160046 31.5 14.0 34.0 2.0 35.0 96-97 23.724554805982585 31.0 2.0 34.0 2.0 35.0 98-99 22.801945061226824 31.0 2.0 34.0 2.0 35.0 100-101 19.80309377384924 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31030878029530484 1101 2 0.43270444550559617 1101 3 0.3927367236287793 1101 4 0.23605336261547194 1101 5 0.16147533745657938 1101 6 0.24970517506478984 1101 7 0.15815308104966164 1101 8 0.29958806944020466 1101 9 0.42937571656094775 1101 10-11 0.35513201348760504 1101 12-13 0.42804269530298455 1101 14-15 0.6020419908237287 1101 16-17 0.5663257123163277 1101 18-19 0.5018119932439973 1101 20-21 0.4773923840785912 1101 22-23 0.4934850040732286 1101 24-25 0.3852827568968209 1101 26-27 0.48370621123115143 1101 28-29 0.38941932592128836 1101 30-31 0.5967103111888221 1101 32-33 0.47976030072478437 1101 34-35 0.6548848346230116 1101 36-37 0.4652778381206417 1101 38-39 0.37968265015550173 1101 40-41 0.4221387017479188 1101 42-43 0.44981994009643955 1101 44-45 0.5588289935802138 1101 46-47 0.5222668501592906 1101 48-49 0.48899254226318334 1101 50-51 0.6186913688461289 1101 52-53 0.7056737954023333 1101 54-55 0.724023014221423 1101 56-57 0.8759176134659512 1101 58-59 0.8343405500120262 1101 60-61 0.5862671582157972 1101 62-63 0.7319391877753176 1101 64-65 0.9319197088505682 1101 66-67 0.912294850454451 1101 68-69 0.9679116630512326 1101 70-71 1.1539889557241452 1101 72-73 1.01203433674182 1101 74-75 0.7664243260938193 1101 76-77 0.7440292034926514 1101 78-79 0.8032416626162195 1101 80-81 0.8541722164413947 1101 82-83 1.0373640149380208 1101 84-85 0.8729660972810684 1101 86-87 0.9166486637110864 1101 88-89 0.8537311830524246 1101 90-91 0.8992228411049688 1101 92-93 0.7315215448466823 1101 94-95 1.0720518447062979 1101 96-97 0.9153712186722736 1101 98-99 1.2645933822003599 1101 100-101 1.6194754467628876 1102 1 0.34848072664746965 1102 2 0.43404204573571903 1102 3 0.3571105325532997 1102 4 0.25627557684725133 1102 5 0.1165003275684029 1102 6 0.24149978870687505 1102 7 0.23410144680422462 1102 8 0.3107347380245784 1102 9 0.39276430381059413 1102 10-11 0.45741528269762455 1102 12-13 0.4493508107968651 1102 14-15 0.5683646689007489 1102 16-17 0.6204805431386688 1102 18-19 0.5309227767469267 1102 20-21 0.35969794662577925 1102 22-23 0.3372087105203221 1102 24-25 0.3202077865613475 1102 26-27 0.19335706880549708 1102 28-29 0.32801196912002695 1102 30-31 0.30838195190652584 1102 32-33 0.3281979692732051 1102 34-35 0.42016835553200593 1102 36-37 0.44628175743314813 1102 38-39 0.6107638770081891 1102 40-41 0.7215885597177873 1102 42-43 0.8176420248830354 1102 44-45 0.7453235460953991 1102 46-47 0.6866766160792466 1102 48-49 0.8610179998159495 1102 50-51 0.6539723727654447 1102 52-53 0.5754878977359397 1102 54-55 0.5922082646618989 1102 56-57 0.6239633507641429 1102 58-59 0.4293209534495226 1102 60-61 0.5224111300255103 1102 62-63 0.3892726169939742 1102 64-65 0.6331494380943923 1102 66-67 0.5892967561752087 1102 68-69 0.3021499141029693 1102 70-71 0.38900028217770455 1102 72-73 0.4981621279035906 1102 74-75 0.16693491542897476 1102 76-77 0.30684247881536564 1102 78-79 0.2657780690740985 1102 80-81 0.4168776207778109 1102 82-83 0.1578451195369226 1102 84-85 0.20804933772549816 1102 86-87 0.34718334423318353 1102 88-89 0.2536089292679513 1102 90-91 0.6603784989381971 1102 92-93 0.7061179275729437 1102 94-95 0.6691141581793865 1102 96-97 0.589205313095345 1102 98-99 0.5570567959575037 1102 100-101 1.2990374905585078 1103 1 0.21945802521097946 1103 2 0.26539000811816393 1103 3 0.37613288950669244 1103 4 0.16451905750452767 1103 5 -0.010090323160177661 1103 6 0.1922662636112804 1103 7 0.2021747528085598 1103 8 0.2855874714980047 1103 9 0.1620226316896094 1103 10-11 0.03106921497224846 1103 12-13 0.1742672736443538 1103 14-15 0.11614103047021018 1103 16-17 0.41682557741474113 1103 18-19 0.1578165726062366 1103 20-21 0.2120628231285906 1103 22-23 0.004758402207947654 1103 24-25 0.1177619565293071 1103 26-27 0.2547818509199402 1103 28-29 0.2585538410412056 1103 30-31 0.17976229570065527 1103 32-33 0.30218605015783595 1103 34-35 0.2719648349146908 1103 36-37 0.4055018476877166 1103 38-39 0.377345571176086 1103 40-41 0.5155015432859429 1103 42-43 0.6250242977825522 1103 44-45 0.955151649026952 1103 46-47 1.0349111401758364 1103 48-49 0.8418440702971921 1103 50-51 1.1045631612872668 1103 52-53 0.9920334211780144 1103 54-55 1.12240565347998 1103 56-57 1.0061122363613038 1103 58-59 1.2381156070717587 1103 60-61 0.9833846392748882 1103 62-63 1.0568843156401613 1103 64-65 1.1543833765697116 1103 66-67 1.1625373087261082 1103 68-69 1.238454988339079 1103 70-71 1.210210106835806 1103 72-73 1.0771589931073535 1103 74-75 1.1082212153359663 1103 76-77 0.813401437098463 1103 78-79 0.9581925511437532 1103 80-81 1.2778580196892442 1103 82-83 1.207285334494376 1103 84-85 1.0870840960706012 1103 86-87 0.8609488890332102 1103 88-89 0.9023395090330126 1103 90-91 0.9372395689027151 1103 92-93 1.137152410331563 1103 94-95 1.321940642040353 1103 96-97 1.4577850092612472 1103 98-99 1.289331417887297 1103 100-101 1.389147380668394 1104 1 0.28153279254890506 1104 2 0.4034624619800944 1104 3 0.29619177798663543 1104 4 0.2779740235910708 1104 5 0.18933130784039776 1104 6 0.2852785145182679 1104 7 0.33189766147884114 1104 8 0.27486135550872604 1104 9 0.49974806415188766 1104 10-11 0.4105371154927795 1104 12-13 0.264098495054526 1104 14-15 0.3874539280988998 1104 16-17 0.4006417612309221 1104 18-19 0.2765399544875109 1104 20-21 0.33478220900587274 1104 22-23 0.32835585738434503 1104 24-25 0.23917212530789556 1104 26-27 0.3616013472131101 1104 28-29 0.4254711342742894 1104 30-31 0.5134023398670209 1104 32-33 0.5051725373548521 1104 34-35 0.287601829499053 1104 36-37 0.3434533698497617 1104 38-39 0.16329646239013584 1104 40-41 0.30968104799176643 1104 42-43 0.4642797791432969 1104 44-45 0.35556702312654664 1104 46-47 0.30544061070531114 1104 48-49 0.3974358469416046 1104 50-51 0.40740768518441683 1104 52-53 0.41596212405408295 1104 54-55 0.6652890858370952 1104 56-57 0.5562988918111174 1104 58-59 0.531432816178441 1104 60-61 0.3095584654487098 1104 62-63 0.5595816183374609 1104 64-65 0.6655721877585208 1104 66-67 0.6436772214449675 1104 68-69 0.8605749736169628 1104 70-71 0.5929694527530458 1104 72-73 0.6127023970718071 1104 74-75 0.5191681631250162 1104 76-77 0.4259152390859491 1104 78-79 0.3934467706085023 1104 80-81 0.658556268808546 1104 82-83 0.7123434342893162 1104 84-85 0.6868317168708522 1104 86-87 0.49801182609614614 1104 88-89 0.5942528588410987 1104 90-91 0.9198359199201214 1104 92-93 0.6964142011722991 1104 94-95 0.7022392907600512 1104 96-97 0.6943011247247313 1104 98-99 1.2078970627687333 1104 100-101 1.3844783654730648 1105 1 0.05207746057013907 1105 2 0.20694844967660586 1105 3 0.037129427215560895 1105 4 0.05267884292458547 1105 5 -0.04068301813643416 1105 6 0.09633479781680165 1105 7 0.06871916729818395 1105 8 0.11365666258089391 1105 9 -0.2344507579344608 1105 10-11 -0.14445731329401212 1105 12-13 -0.11994536303402725 1105 14-15 0.07554464055875343 1105 16-17 0.04922742214534992 1105 18-19 0.06711617711132334 1105 20-21 0.2766660281427562 1105 22-23 0.12208285857349921 1105 24-25 0.11895938923358784 1105 26-27 0.24375520633164172 1105 28-29 0.30992370405490277 1105 30-31 0.22239774244569332 1105 32-33 0.5667194619515215 1105 34-35 0.5294402362257031 1105 36-37 0.31507857233293635 1105 38-39 0.47042714713438016 1105 40-41 0.4157143441836766 1105 42-43 0.3804161661881196 1105 44-45 0.6335143821842095 1105 46-47 0.8686322136229379 1105 48-49 0.7984901639295856 1105 50-51 1.0957329504022582 1105 52-53 1.044929155580597 1105 54-55 1.2941517156369997 1105 56-57 0.9196918074751181 1105 58-59 1.1670394229818832 1105 60-61 1.10487958268763 1105 62-63 1.0655058311109826 1105 64-65 1.0195216629408748 1105 66-67 1.0717753309778288 1105 68-69 1.0334408244208078 1105 70-71 0.8158441987712663 1105 72-73 0.9037023128013573 1105 74-75 0.8072345307876354 1105 76-77 0.6632194273274123 1105 78-79 0.8398451451251141 1105 80-81 0.9174444606455481 1105 82-83 0.7143570299222333 1105 84-85 0.7102930788686272 1105 86-87 0.5317335837905084 1105 88-89 0.8884527108996387 1105 90-91 1.2062992763185854 1105 92-93 1.0774655218775528 1105 94-95 0.9034687030204687 1105 96-97 1.0831151585639986 1105 98-99 1.3990299385447216 1105 100-101 1.0000159641397204 1106 1 0.11445911563627931 1106 2 0.1450527667132384 1106 3 0.15614315569427362 1106 4 0.11102441106422134 1106 5 0.05115824714463457 1106 6 0.09766305937986175 1106 7 0.09945573683883424 1106 8 0.05510775102781906 1106 9 0.06531381143455661 1106 10-11 0.15564911552927185 1106 12-13 0.07928361097719261 1106 14-15 0.19004377215178891 1106 16-17 0.3014823886683047 1106 18-19 0.09488141434219699 1106 20-21 0.21123095718709806 1106 22-23 0.13188485059273347 1106 24-25 0.03218118839372863 1106 26-27 0.06980191188851848 1106 28-29 0.07102830651853509 1106 30-31 0.42968396602270786 1106 32-33 0.43845873838476734 1106 34-35 0.4687832691020546 1106 36-37 0.1916108768853988 1106 38-39 0.39253962402504783 1106 40-41 0.3302611402190365 1106 42-43 0.2742368583392505 1106 44-45 0.3537744751998346 1106 46-47 0.09292652802916734 1106 48-49 0.18789839728574265 1106 50-51 0.1493824982896328 1106 52-53 0.07285203059529266 1106 54-55 0.21424708183237584 1106 56-57 0.22405492284545403 1106 58-59 0.12048823920607177 1106 60-61 0.10338808673304811 1106 62-63 0.02372062518380602 1106 64-65 0.18859844653311342 1106 66-67 0.28494778671332455 1106 68-69 0.38187162889585125 1106 70-71 0.349979774727494 1106 72-73 0.09433676290666426 1106 74-75 0.26718427325040395 1106 76-77 0.050937018771318066 1106 78-79 0.09482379406670205 1106 80-81 0.17095586559929288 1106 82-83 0.45556755751444555 1106 84-85 0.35260408609011407 1106 86-87 0.5154050144140285 1106 88-89 0.656312418868648 1106 90-91 0.6846404044827672 1106 92-93 0.12884517895474445 1106 94-95 0.5301861620113257 1106 96-97 0.4965155827868628 1106 98-99 0.7195084858146039 1106 100-101 0.9307411539113595 1107 1 0.13212367281638393 1107 2 0.22634471606082585 1107 3 0.1472172268560925 1107 4 0.10920088170442455 1107 5 0.03268617744765123 1107 6 0.1403543201330706 1107 7 0.0366535270463757 1107 8 0.10439516982377484 1107 9 0.05662200821669927 1107 10-11 0.1742453159796753 1107 12-13 0.190973459025237 1107 14-15 0.2695599007565477 1107 16-17 0.3745485773064132 1107 18-19 0.22718094317542636 1107 20-21 0.12588995984729223 1107 22-23 0.19435795927738297 1107 24-25 0.2244504487599741 1107 26-27 0.1898407679065599 1107 28-29 -0.0012104910122801016 1107 30-31 0.07899559252432198 1107 32-33 0.38528969350244324 1107 34-35 0.38862528290044196 1107 36-37 0.2293165871651084 1107 38-39 0.2803461850200719 1107 40-41 0.3805822457160488 1107 42-43 0.2265432607472988 1107 44-45 0.49645372044951586 1107 46-47 0.4403972203607154 1107 48-49 0.6561562641274605 1107 50-51 0.7564052950571067 1107 52-53 0.8461371487757177 1107 54-55 0.9039674651622747 1107 56-57 0.6932744717163075 1107 58-59 0.751602120558271 1107 60-61 0.626687119926757 1107 62-63 0.7145820396029414 1107 64-65 0.8852789869833941 1107 66-67 1.0356040010699061 1107 68-69 1.1688619307942147 1107 70-71 0.8720542867522738 1107 72-73 0.7438356899045289 1107 74-75 0.8742985115337447 1107 76-77 0.7442717715832536 1107 78-79 0.7834636655714959 1107 80-81 0.9943403325570976 1107 82-83 0.9287084896524718 1107 84-85 0.9716874445293691 1107 86-87 1.0854412641279936 1107 88-89 1.2998636716174161 1107 90-91 1.1586025432777411 1107 92-93 1.306489097740542 1107 94-95 1.5712274997609441 1107 96-97 1.4859556338174151 1107 98-99 1.6826730160481702 1107 100-101 1.682209739995816 1108 1 0.10157131633162209 1108 2 0.08680664295392049 1108 3 -0.08229471230326268 1108 4 -0.03213539098815943 1108 5 -0.15317150668253987 1108 6 0.02062540447042238 1108 7 0.09459115993085021 1108 8 0.09944181568322819 1108 9 0.13186528903226957 1108 10-11 0.07535030153274391 1108 12-13 0.024336322242824338 1108 14-15 -8.741020675984146E-5 1108 16-17 0.12633527727369653 1108 18-19 0.08695544843629222 1108 20-21 0.13291494894395584 1108 22-23 0.2110034439309274 1108 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2316 82-83 -1.427130249921209 2316 84-85 -1.063322788711286 2316 86-87 -1.3848953708735507 2316 88-89 -0.9113566906006874 2316 90-91 -0.8101880035248534 2316 92-93 -0.8516868753827218 2316 94-95 -1.2181728902606785 2316 96-97 -1.1374572484810095 2316 98-99 -1.4272155767846968 2316 100-101 -1.5153970332510163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 25.0 5 65.0 6 162.0 7 363.0 8 704.0 9 1397.0 10 2375.0 11 3999.0 12 5845.0 13 7911.0 14 9783.0 15 13259.0 16 17150.0 17 22273.0 18 18143.0 19 9797.0 20 7906.0 21 8058.0 22 8638.0 23 9413.0 24 10580.0 25 12564.0 26 15178.0 27 18167.0 28 22292.0 29 26822.0 30 32525.0 31 39288.0 32 48083.0 33 59854.0 34 78122.0 35 102824.0 36 136318.0 37 164946.0 38 93416.0 39 4617.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.45497033051256 19.248919493366834 15.01558536990068 19.280524806219926 2 16.022768225194138 22.473995744533802 39.1568055370428 22.346430493229267 3 19.579040073931605 29.166452744197436 30.201969139873704 21.05253804199726 4 12.241537108177512 17.78266162353645 36.93046344865989 33.04533781962615 5 11.989925626957932 39.96832752967566 32.24954510315767 15.792201740208734 6 28.24701738034966 37.37347808401489 17.864519364813578 16.514985170821863 7 26.791414125956557 35.86234404510733 20.37846798167194 16.967773847264166 8 28.03569647922296 31.847822974844824 19.537888758018035 20.57859178791418 9 28.79252808877831 15.92826254368817 20.64066109827617 34.63854826925735 10-11 24.951474331254083 27.778254971536214 25.475383713146655 21.794886984063044 12-13 28.00780162430173 25.774189280714328 26.899905811825057 19.318103283158877 14-15 24.022135667352853 25.670561635788296 28.487774536083997 21.819528160774848 16-17 20.201403376436428 29.253990993668317 28.786544410140564 21.75806121975469 18-19 20.016132328534773 25.980453620542754 34.21569140454924 19.787722646373236 20-21 22.003005312610764 24.12414981937518 34.30504120457353 19.56780366344053 22-23 21.15204336268114 24.639421830918124 34.32503745609553 19.883497350305202 24-25 20.93024059427348 24.87592151783008 34.68925337680071 19.504584511095732 26-27 20.553979397444106 24.822935215047263 34.97033822372933 19.65274716377931 28-29 20.069469436558045 25.21861002873661 34.9749881395451 19.736932395160242 30-31 20.20395590334753 25.066000833279034 34.76165652712205 19.96838673625139 32-33 19.76679150987219 25.44017226289047 34.52692125992415 20.266114967313197 34-35 20.589696018831095 24.922713723337345 34.564318671238844 19.92327158659272 36-37 20.918889003536353 24.645858645600935 34.18384198134516 20.251410369517554 38-39 20.644571737757783 25.04733181838602 34.37482258281896 19.933273861037236 40-41 20.352449786667073 25.362354970388118 34.076102812499535 20.20909243044527 42-43 20.24867986318066 25.74623458030337 33.026547868750974 20.978537687764995 44-45 20.60216644065638 25.577140758417134 32.68706074238332 21.133632058543164 46-47 21.328805614134854 26.174383337677416 31.651580851282297 20.845230196905433 48-49 21.418257732116526 26.609548416651897 30.555466424845452 21.416727426386124 50-51 21.32098021299062 26.972592007890515 30.27259896836833 21.433828810750533 52-53 21.599960506991497 27.456625569254694 29.47190442691942 21.471509496834386 54-55 21.04263105332868 26.999979759913078 29.7862498722653 22.171139314492937 56-57 21.685386768846023 27.373539962847758 28.891025853042795 22.05004741526342 58-59 22.136903016498778 26.673449420109137 29.222017192848394 21.9676303705437 60-61 21.724039368644394 27.133550183074956 29.87180063119763 21.270609817083013 62-63 21.98436049841038 27.1806441420983 29.820896111846135 21.014099247645188 64-65 21.598091188879685 27.37557231256239 29.783111188346496 21.24322531021143 66-67 21.77500888590498 27.493927964930293 29.084208759527662 21.64685438963706 68-69 21.87490743794146 27.34633710953621 29.498269212921368 21.28048623960096 70-71 21.97175756486746 27.42767355828421 29.221333770373974 21.379235106474358 72-73 21.824723147955716 27.393160262444827 29.113979664524386 21.66813692507507 74-75 21.32168720438107 28.475387572119786 28.776628227463846 21.426296996035294 76-77 21.297286356747577 27.986957530520463 29.13965118058538 21.576104932146578 78-79 21.12848978334501 27.69799320025098 29.280289959386135 21.893227057017874 80-81 21.22409949270254 28.407028706045907 28.617431759704115 21.751440041547447 82-83 21.371319681680852 27.89725716316818 28.645465136944377 22.085958018206593 84-85 20.9818387186591 27.735102397873646 28.471565361458275 22.811493522008977 86-87 21.08755181621479 27.919665058802796 28.221491270291445 22.77129185469097 88-89 21.081335933450173 27.62780479375972 28.314630594159613 22.976228678630495 90-91 20.85214789653759 27.298503557199073 28.707559233165636 23.141789313097703 92-93 21.02571190912838 27.670674305121896 28.66765561789294 22.63595816785678 94-95 20.68996668792689 27.212922793790963 28.86653054857303 23.230579969709115 96-97 20.548870970449055 27.284839676915198 28.94795040977982 23.21833894285593 98-99 20.530608839630087 27.33560648732545 28.488457910037802 23.645326763006665 100-101 20.465766314138023 27.19851544022144 28.750263495537975 23.58545475010256 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1549.0 1 1174.5 2 1726.0 3 2284.5 4 1536.5 5 1248.5 6 1745.5 7 3211.0 8 4700.0 9 5223.5 10 4597.5 11 3574.5 12 3278.0 13 3373.5 14 3487.5 15 3506.5 16 3523.0 17 3608.5 18 3681.5 19 3783.0 20 4041.5 21 4305.0 22 4534.5 23 4971.5 24 5535.0 25 6190.5 26 6956.0 27 7785.0 28 8787.5 29 10193.5 30 12239.5 31 14397.5 32 17316.5 33 19897.5 34 21593.0 35 23375.5 36 26043.5 37 28586.5 38 28491.5 39 27618.5 40 27207.5 41 27415.0 42 27579.0 43 28709.5 44 30468.0 45 31118.0 46 30651.0 47 33413.0 48 44016.5 49 43894.5 50 34818.0 51 30247.0 52 27042.0 53 26357.0 54 25947.0 55 24813.5 56 22967.5 57 21331.5 58 20746.0 59 19932.0 60 18371.0 61 16787.0 62 14647.5 63 10827.0 64 7250.5 65 5579.5 66 4188.5 67 3120.5 68 2279.5 69 1570.0 70 1234.0 71 977.5 72 863.5 73 926.5 74 778.5 75 618.5 76 463.5 77 261.5 78 176.0 79 134.5 80 93.0 81 62.0 82 39.0 83 22.5 84 15.0 85 6.5 86 5.5 87 2.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03801068840812224 2 0.005726285526418415 3 0.004442807736014288 4 9.872906080031752E-4 5 5.923743648019052E-4 6 4.936453040015876E-4 7 0.0 8 0.0 9 0.0012834777904041277 10-11 6.911034256022226E-4 12-13 6.911034256022226E-4 14-15 0.0027644137024088905 16-17 0.0029618718240095256 18-19 0.0 20-21 0.0 22-23 0.002023945746406509 24-25 5.923743648019052E-4 26-27 0.0037023397800119064 28-29 0.0052820047528169866 30-31 6.911034256022226E-4 32-33 2.468226520007938E-4 34-35 0.007898324864025402 36-37 0.010514644975233816 38-39 0.00755277315122429 40-41 0.0019252166856061914 42-43 4.442807736014288E-4 44-45 0.0013328423208042864 46-47 9.872906080031752E-5 48-49 3.455517128011113E-4 50-51 0.0011353841992036513 52-53 0.003504881658411272 54-55 0.0031099654152100015 56-57 0.0013328423208042864 58-59 9.872906080031752E-4 60-61 0.0036036107192115893 62-63 0.005232640222416828 64-65 0.009477989836830483 66-67 0.003258059006410478 68-69 0.0038504333712123828 70-71 0.0 72-73 0.0012341132600039689 74-75 0.006071837239219527 76-77 0.0027644137024088905 78-79 0.004097256023213177 80-81 0.005430098344017463 82-83 0.005232640222416828 84-85 0.005825014587218734 86-87 0.0038010688408122244 88-89 0.009675447958431116 90-91 0.013624610390443817 92-93 0.0015303004424049214 94-95 0.002665684641608573 96-97 0.0011847487296038103 98-99 0.0036036107192115893 100-101 0.004689630388015082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.62349274417264 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78307348766724 34.57488503395688 2 12.946885861157337 11.81364306292286 3 4.463145909447665 6.10872915047608 4 2.0191653727176835 3.6848550692587954 5 1.1223652440034668 2.5603111283051856 6 0.7151460782398789 1.9576477146960376 7 0.4806788576898697 1.5351173863263672 8 0.3426980128985407 1.2508064243935162 9 0.27271327986080957 1.119791911047225 >10 1.5905990701629231 14.250185573070414 >50 0.16229124655539334 5.1061445901309055 >100 0.09233041060740263 8.203743522223663 >500 0.004562208508006623 1.4824800544313605 >1k 0.0036932164112434564 3.262704983069568 >5k 2.1724802419079159E-4 0.7346488041348697 >10k+ 4.3449604838158317E-4 2.3543055915563356 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12722 1.2560311115016394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11031 1.0890802696883024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7412 0.7317797986519534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4495 0.44378712829742717 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 0.3467364615307151 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2822 0.278613409578496 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.2741706018424817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2112 0.20851577641027058 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2108 0.2081208601670693 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1753 0.1730720435829566 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1676 0.16546990590133215 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1616 0.1595461622533131 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1490 0.1471063005924731 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1443 0.14246603473485817 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1396 0.13782576887724324 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1215 0.11995580887238577 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1139 0.11245240025156164 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1131 0.11166256776515911 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1118 0.11037908997475498 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1115 0.11008290279235403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.872906080031751E-5 0.0 3 0.0 0.0 0.0 9.872906080031751E-5 0.0 4 0.0 0.0 0.0 9.872906080031751E-5 0.0 5 0.0 0.0 0.0 9.872906080031751E-5 0.0 6 0.0 0.0 0.0 9.872906080031751E-5 0.0 7 0.0 0.0 0.0 9.872906080031751E-5 0.0 8 0.0 0.0 0.0 9.872906080031751E-5 0.0 9 0.0 0.0 0.0 9.872906080031751E-5 0.0 10-11 0.0 0.0 0.0 9.872906080031751E-5 0.0 12-13 0.0 0.0 0.0 2.961871824009525E-4 0.0 14-15 0.0 0.0 0.0 3.9491624320127003E-4 0.0 16-17 0.0 0.0 0.0 3.9491624320127003E-4 0.0 18-19 0.0 0.0 0.0 6.911034256022226E-4 0.0 20-21 0.0 0.0 0.0 6.911034256022226E-4 0.0 22-23 0.0 0.0 0.0 9.872906080031752E-4 0.0 24-25 0.0 0.0 0.0 0.00118474872960381 0.0 26-27 0.0 0.0 0.0 0.0013822068512044452 0.0 28-29 0.0 0.0 0.0 0.002418861989607779 0.0 30-31 0.0 0.0 0.0 0.005084546631216352 0.0 32-33 0.0 0.0 0.0 0.007997053924825718 0.0 34-35 0.0 0.0 0.0 0.011156383870435879 0.0 36-37 0.0 0.0 0.0 0.015154910832848738 0.0 38-39 0.0 0.0 0.0 0.018462334369659376 0.0 40-41 0.0 0.0 0.0 0.02354688100087573 0.0 42-43 0.0 0.0 0.0 0.030803466969699066 0.0 44-45 0.0 0.0 0.0 0.04126874741453272 0.0 46-47 0.0 0.0 0.0 0.07029509128982607 0.0 48-49 0.0 0.0 0.0 0.12429988754759975 0.0 50-51 0.0 0.0 0.0 0.16428515717172834 0.0 52-53 0.0 0.0 0.0 0.20086427419824598 0.0 54-55 0.0 0.0 0.0 0.2454898096799895 0.0 56-57 0.0 0.0 0.0 0.2820689267065072 0.0 58-59 0.0 0.0 0.0 0.32768175279625383 0.0 60-61 0.0 0.0 0.0 0.4138228583445308 0.0 62-63 0.0 0.0 0.0 0.4803662453239449 0.0 64-65 0.0 0.0 0.0 0.5537219374985808 0.0 66-67 0.0 0.0 0.0 0.6863150661534072 0.0 68-69 0.0 0.0 0.0 0.8279419038714626 0.0 70-71 0.0 0.0 0.0 0.9534265401486662 0.0 72-73 0.0 0.0 0.0 1.087747427367498 0.0 74-75 0.0 0.0 0.0 1.2015820344702641 0.0 76-77 0.0 0.0 0.0 1.2962138392473688 0.0 78-79 0.0 0.0 0.0 1.4123685792789422 0.0 80-81 0.0 0.0 0.0 1.617231380439601 0.0 82-83 0.0 0.0 0.0 1.8444069493411317 0.0 84-85 0.0 0.0 0.0 2.0891069265347184 0.0 86-87 0.0 0.0 0.0 2.471435214483948 0.0 88-89 0.0 0.0 0.0 2.9165551851021796 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 70 4.310119E-6 40.713696 3 TACCTGG 1040 0.0 38.364826 2 TCACGCA 90 5.6977296E-7 36.94391 4 TATCACG 85 1.6321743E-5 33.528923 2 GTACCTG 1315 0.0 30.352255 1 CGCGGAA 110 7.6397555E-11 25.908075 82-83 ACCTGGG 1550 0.0 25.435112 3 GTATCAA 19160 0.0 24.204254 1 GTACACG 200 2.0461812E-8 23.757866 1 CACGCAG 160 1.8594274E-6 23.749655 5 GAGTACT 11120 0.0 23.621508 12-13 GTACATG 11420 0.0 23.591436 1 CGTCACT 285 3.6379788E-12 23.34106 1 TACATGG 11610 0.0 23.23825 2 ATGGGAG 2740 0.0 23.22959 5 GGTATCA 14315 0.0 22.670795 1 ACATGGG 11540 0.0 22.226713 3 AGTACTT 11255 0.0 22.156496 12-13 AATTCGC 130 6.2573235E-10 21.921677 78-79 GTACTTT 11970 0.0 21.745716 14-15 >>END_MODULE