FastQCFastQC Report
Thu 26 May 2016
SRR936488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038916
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107621.035887405719038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84350.8119039460360608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.51370851926431No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39970.3847279279556769No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24140.23235757270077656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24050.23149128514721112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22410.21570560083779633No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.1981873414212506No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18630.17932152358804754No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17050.16411336431434304No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16730.1610332307905548No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14320.13783597518952445No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13740.13225323317765825No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.13109818310623766No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12100.11646754886824344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC451.5074016E-552.8277361
TATCACG851.2732926E-1050.3368762
ATCACGC902.237357E-1047.5472533
CGTATCA608.2487946E-539.616982
TACCTGG9850.036.6809042
TATAACG801.0712505E-535.655282
AGGTGCG702.0400825E-433.9655959
TCACGCA1308.201823E-932.9189154
TAGCGCG953.4657696E-530.0312944
CGAATAC650.005814120629.2612574
AGCGCGC1153.8328562E-628.9432015
CGCGCGT1004.917581E-528.5297267
GTACCTG12750.028.3405251
GCGCGCG1056.856367E-527.1711676
GTACATG125300.027.1306041
TACATGG126900.026.2989332
ACATGGG126500.025.1831323
ACCTGGG14050.025.042683
GTATCAA174900.024.5744111
GAGTACT80850.023.8160112-13
Produced by FastQC (version 0.11.5)