##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1038916 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44671465258019 31.0 31.0 34.0 30.0 34.0 2 31.433196716577665 31.0 31.0 34.0 28.0 34.0 3 31.34094575499848 31.0 31.0 34.0 28.0 34.0 4 35.064695317041995 37.0 35.0 37.0 32.0 37.0 5 35.1217557531119 37.0 35.0 37.0 32.0 37.0 6 35.23441452436963 37.0 35.0 37.0 32.0 37.0 7 35.1113487519684 37.0 35.0 37.0 32.0 37.0 8 35.104198992026305 37.0 35.0 37.0 32.0 37.0 9 36.57163716797123 39.0 37.0 39.0 32.0 39.0 10-11 36.4421897439254 39.0 37.0 39.0 32.0 39.0 12-13 36.348582561054016 39.0 36.0 39.0 32.0 39.0 14-15 37.2378469481652 39.0 36.5 41.0 31.5 41.0 16-17 37.21361592275025 39.0 36.5 41.0 32.0 41.0 18-19 37.201364691659386 39.5 36.5 41.0 31.0 41.0 20-21 37.250415818025715 39.0 36.5 41.0 31.0 41.0 22-23 37.22446473054607 39.0 36.0 41.0 31.0 41.0 24-25 37.29707214057729 39.0 36.0 41.0 31.5 41.0 26-27 37.20455696129427 39.0 36.0 41.0 31.0 41.0 28-29 37.084406727781655 39.0 36.0 41.0 31.0 41.0 30-31 36.81066034212583 39.0 35.5 41.0 30.5 41.0 32-33 36.52505014842393 39.0 35.0 40.5 30.0 41.0 34-35 36.277332334856716 38.0 35.0 40.0 30.0 41.0 36-37 36.01919885727047 38.0 35.0 40.0 30.0 41.0 38-39 35.77529559656411 38.0 35.0 40.0 29.0 41.0 40-41 35.48460703271486 38.0 35.0 40.0 28.0 41.0 42-43 35.10319169210985 38.0 34.5 40.0 26.5 41.0 44-45 34.72991560434145 38.0 34.0 40.0 24.5 41.0 46-47 34.26871277369874 38.0 34.0 40.0 22.5 41.0 48-49 33.83763942416904 38.0 33.0 40.0 19.0 41.0 50-51 32.77647471017868 37.0 32.0 39.5 15.5 40.5 52-53 32.84355328053471 37.0 32.5 39.5 14.5 40.5 54-55 33.25655298407186 38.0 33.0 40.0 13.5 41.0 56-57 33.100914318385705 37.5 33.0 40.0 11.0 41.0 58-59 32.8661864866842 37.0 33.0 40.0 8.5 41.0 60-61 32.562721625232456 36.5 32.0 40.0 7.5 41.0 62-63 32.23675205695167 36.0 32.0 39.5 6.5 41.0 64-65 31.871721101609758 35.5 31.0 39.0 2.0 41.0 66-67 31.51124778134132 35.0 31.0 39.0 2.0 40.5 68-69 31.092585926099897 35.0 31.0 38.5 2.0 40.0 70-71 30.697174747525306 35.0 30.0 37.5 2.0 40.0 72-73 30.294586376569427 35.0 30.0 37.0 2.0 39.0 74-75 29.9071676632182 35.0 29.5 36.5 2.0 39.0 76-77 29.475268452887434 34.0 29.0 36.0 2.0 39.0 78-79 29.03227017391204 34.0 29.0 35.5 2.0 37.5 80-81 28.60065587593222 34.0 28.5 35.0 2.0 37.0 82-83 28.214230986913286 34.0 27.5 35.0 2.0 36.5 84-85 27.849692852935178 34.0 27.0 35.0 2.0 36.0 86-87 27.51981295889177 34.0 26.0 35.0 2.0 36.0 88-89 27.17200765028164 34.0 26.0 35.0 2.0 35.5 90-91 26.799872174458763 33.0 25.0 35.0 2.0 35.0 92-93 26.39208607818149 33.0 24.5 35.0 2.0 35.0 94-95 25.923822041435496 33.0 23.5 35.0 2.0 35.0 96-97 25.353200836256253 33.0 19.5 35.0 2.0 35.0 98-99 24.679349437298107 32.5 8.5 35.0 2.0 35.0 100-101 22.28563136961987 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5291124503937361 1101 2 0.5295271921597333 1101 3 0.6098148293051189 1101 4 0.6197060206986222 1101 5 0.6056585570409325 1101 6 0.528665164049194 1101 7 0.5965709118550251 1101 8 0.5659828568726795 1101 9 0.6838833527373183 1101 10-11 0.7555871653725461 1101 12-13 0.8614722427970776 1101 14-15 1.0749268800965268 1101 16-17 1.0733104707575123 1101 18-19 1.1631866059420801 1101 20-21 1.0829701599223398 1101 22-23 0.984578686512144 1101 24-25 0.8948596238588138 1101 26-27 0.8025829203053192 1101 28-29 0.809692086041963 1101 30-31 0.7453186259172142 1101 32-33 0.653749267724578 1101 34-35 0.9232960502948373 1101 36-37 0.8873360271797353 1101 38-39 0.8263755433195072 1101 40-41 0.9288203856500274 1101 42-43 1.0014313608027905 1101 44-45 0.9548761738126714 1101 46-47 1.0480304734422958 1101 48-49 0.8002004868971611 1101 50-51 0.9067182051907352 1101 52-53 0.9173503691240512 1101 54-55 0.7109824380060132 1101 56-57 0.8731527411461784 1101 58-59 0.571449087148558 1101 60-61 0.4906240189086688 1101 62-63 0.6933414584035589 1101 64-65 0.5569337260166698 1101 66-67 0.7561236381268763 1101 68-69 0.7990834297485279 1101 70-71 0.6767260482781268 1101 72-73 0.6697385784729164 1101 74-75 0.4819773634433524 1101 76-77 0.5514064331199222 1101 78-79 0.6086146469932636 1101 80-81 0.6288744055669646 1101 82-83 0.697239253522504 1101 84-85 0.661280650855165 1101 86-87 0.7158821012039702 1101 88-89 0.6765368535315517 1101 90-91 0.7097375528591883 1101 92-93 0.8608457809341381 1101 94-95 0.8168629095957023 1101 96-97 0.4607909165530728 1101 98-99 0.4454526921342534 1101 100-101 0.6838293438034917 1102 1 0.33595269944672523 1102 2 0.45244376811654163 1102 3 0.5183934420973841 1102 4 0.405524804170561 1102 5 0.347557803786259 1102 6 0.23493502950832834 1102 7 0.5160967532796761 1102 8 0.1792965243085689 1102 9 0.26149144260539003 1102 10-11 0.42041713881269516 1102 12-13 0.4913254016772086 1102 14-15 0.7792814975911995 1102 16-17 0.4059486037309128 1102 18-19 0.39639566676847693 1102 20-21 0.4235250865562108 1102 22-23 0.35007701019394943 1102 24-25 0.616813405371424 1102 26-27 0.5165850755715766 1102 28-29 0.4131143876050771 1102 30-31 0.4013212601315175 1102 32-33 0.4358915806082777 1102 34-35 0.043021634829166544 1102 36-37 0.18090125119680778 1102 38-39 0.28106760148992294 1102 40-41 0.37145274947638995 1102 42-43 0.2465210547114438 1102 44-45 0.30746521461033893 1102 46-47 0.6068703306286949 1102 48-49 1.1296345662283755 1102 50-51 0.7272607227158758 1102 52-53 0.6146225422420031 1102 54-55 0.8443623962069111 1102 56-57 0.8047448080247435 1102 58-59 0.7792619273105217 1102 60-61 0.6410844621533229 1102 62-63 0.8123039916033648 1102 64-65 0.8054723901870027 1102 66-67 0.5772229327670111 1102 68-69 0.6720010942236456 1102 70-71 0.3587208669309767 1102 72-73 0.44765689605901926 1102 74-75 0.45888444752050717 1102 76-77 0.49423636301934337 1102 78-79 0.3691863368090864 1102 80-81 0.3623153207931544 1102 82-83 0.3635218546458532 1102 84-85 0.4318547354112674 1102 86-87 0.430747995748316 1102 88-89 0.46107826076802283 1102 90-91 0.606969995058865 1102 92-93 0.5405563439158065 1102 94-95 0.3312955738531116 1102 96-97 0.2448408577730845 1102 98-99 0.2462799660489985 1102 100-101 1.0304032324262984 1103 1 0.3474330988888923 1103 2 0.5492911881314058 1103 3 0.5753862248574464 1103 4 0.621579854711598 1103 5 0.4052326106309323 1103 6 0.479574014819363 1103 7 0.5435711264791863 1103 8 0.4683515646701437 1103 9 0.6129191124242936 1103 10-11 0.5165453796995365 1103 12-13 0.5882629099480283 1103 14-15 0.8634900951664477 1103 16-17 0.8697229453740789 1103 18-19 0.9327638376206053 1103 20-21 0.8092768908661938 1103 22-23 0.7103674303004297 1103 24-25 0.564062914212279 1103 26-27 0.6337222031304393 1103 28-29 0.457188412666909 1103 30-31 0.6045303361415719 1103 32-33 0.7550900496168964 1103 34-35 0.6993140704695051 1103 36-37 0.799960261549316 1103 38-39 0.650339362638988 1103 40-41 0.854447954948867 1103 42-43 1.1359532442123452 1103 44-45 0.9612911661357302 1103 46-47 1.0805233330615849 1103 48-49 1.0579197337314525 1103 50-51 1.1767690546071279 1103 52-53 1.3658364433179742 1103 54-55 1.3849260331257867 1103 56-57 1.3131632247109195 1103 58-59 1.2234514892881876 1103 60-61 1.2886688753581268 1103 62-63 1.3903509596500285 1103 64-65 1.4761514622270617 1103 66-67 1.2649882350461858 1103 68-69 1.265127411191795 1103 70-71 1.1769716278459086 1103 72-73 1.3406330659339147 1103 74-75 0.9138734028021211 1103 76-77 0.9670281828021139 1103 78-79 0.9176528005651043 1103 80-81 1.1790747902394934 1103 82-83 1.2216868026796917 1103 84-85 1.198107267552924 1103 86-87 1.35093641762602 1103 88-89 1.2355471472364599 1103 90-91 1.1990630964361486 1103 92-93 1.311107663683309 1103 94-95 1.4582197470261526 1103 96-97 1.5754683199309234 1103 98-99 1.5548821220594604 1103 100-101 2.1133511033914125 1104 1 0.31150940529597193 1104 2 0.29842558565874455 1104 3 0.6231320378568235 1104 4 0.5908240508398777 1104 5 0.5415452298477845 1104 6 0.4797569489687845 1104 7 0.3728935650676206 1104 8 0.42287813680813 1104 9 0.6044946137305587 1104 10-11 0.6985710514585008 1104 12-13 0.7059738892364464 1104 14-15 0.7674191776945207 1104 16-17 0.8543421850921291 1104 18-19 0.7931345841634325 1104 20-21 0.6581551805843446 1104 22-23 0.5564107170935344 1104 24-25 0.6387338709388288 1104 26-27 0.45206097049727134 1104 28-29 0.3266210690803746 1104 30-31 0.615561170073569 1104 32-33 0.7419473743192952 1104 34-35 0.6808339102496817 1104 36-37 0.7421778426644181 1104 38-39 0.48115052314655316 1104 40-41 0.7148815851055659 1104 42-43 0.7521149999228456 1104 44-45 0.9119846961740876 1104 46-47 1.0862052486870155 1104 48-49 1.1088930958515064 1104 50-51 0.8613596155861245 1104 52-53 0.6486078575704468 1104 54-55 0.647729518021336 1104 56-57 0.6703564431895543 1104 58-59 0.7429115858240678 1104 60-61 0.669192390971368 1104 62-63 0.6835010285361633 1104 64-65 0.7563551289134232 1104 66-67 0.8117831715813786 1104 68-69 0.739721954799677 1104 70-71 0.7464399183072246 1104 72-73 0.7785278749868638 1104 74-75 0.8698391521937694 1104 76-77 0.9921763880466088 1104 78-79 0.7463959476803659 1104 80-81 0.8537769029383675 1104 82-83 1.0545140499590175 1104 84-85 0.9242229276688967 1104 86-87 0.8631477484910128 1104 88-89 0.8212070154857649 1104 90-91 1.0006810155983068 1104 92-93 1.0551108535975402 1104 94-95 1.0683942204711663 1104 96-97 1.0193261153076456 1104 98-99 1.1929769200601434 1104 100-101 1.7674345366080644 1105 1 0.36131578627354344 1105 2 0.34573549366215417 1105 3 0.5020751425213135 1105 4 0.5321312180502886 1105 5 0.5312856260503551 1105 6 0.4996910744133345 1105 7 0.40693028287616073 1105 8 0.44551080441533486 1105 9 0.48893491881829476 1105 10-11 0.624893410597906 1105 12-13 0.6393760179434977 1105 14-15 0.8899057125385994 1105 16-17 1.063808942873628 1105 18-19 1.04611648243813 1105 20-21 0.8865535250865904 1105 22-23 0.8829187043374631 1105 24-25 0.6552603750683872 1105 26-27 0.6767274644866319 1105 28-29 1.0108475482268346 1105 30-31 0.6798265199839264 1105 32-33 0.848956327821341 1105 34-35 0.5968580887465791 1105 36-37 0.6755211876075435 1105 38-39 0.41228718070173187 1105 40-41 0.6216441060472846 1105 42-43 0.34479541096830957 1105 44-45 0.6319475705531872 1105 46-47 0.46066655060042905 1105 48-49 0.6787337183715749 1105 50-51 0.42526767043138136 1105 52-53 0.6559310286605893 1105 54-55 0.7196881930352959 1105 56-57 0.6382008058265924 1105 58-59 0.34305432015084847 1105 60-61 0.23073256232603256 1105 62-63 0.7221205836900353 1105 64-65 0.6594133542315923 1105 66-67 0.4274177239430159 1105 68-69 0.4685193859491896 1105 70-71 0.4177298361569157 1105 72-73 0.277720975977374 1105 74-75 0.3486655159770926 1105 76-77 0.4487994812482654 1105 78-79 0.274441773298328 1105 80-81 0.3828174600614851 1105 82-83 0.41570675415912106 1105 84-85 0.46462528416303783 1105 86-87 0.5944471635925623 1105 88-89 0.7434494140102892 1105 90-91 0.5905466946977391 1105 92-93 0.553426790610736 1105 94-95 0.8347797354169373 1105 96-97 1.0283719262296707 1105 98-99 1.1413679900716076 1105 100-101 1.287096787144467 1106 1 0.27879211646035174 1106 2 0.22600096408197246 1106 3 0.2824109491375211 1106 4 0.3913020304776538 1106 5 0.4329690883629098 1106 6 0.33404756022562054 1106 7 0.6116148152910483 1106 8 0.4318661138237303 1106 9 0.5599603036299285 1106 10-11 0.6485229177838931 1106 12-13 0.5937217141478186 1106 14-15 0.5672747313043658 1106 16-17 0.3992807091802888 1106 18-19 0.48303758187969237 1106 20-21 0.31657122429570705 1106 22-23 0.11540463709818738 1106 24-25 -0.14754580884747526 1106 26-27 -0.09628637951937691 1106 28-29 0.16209871754395522 1106 30-31 0.12032599556291501 1106 32-33 0.29257619699955484 1106 34-35 0.40301232876132786 1106 36-37 -0.015737901175512548 1106 38-39 0.14863983257122015 1106 40-41 0.16947714882302733 1106 42-43 0.2943256466743023 1106 44-45 0.24461969048969223 1106 46-47 0.41334774160306864 1106 48-49 0.2769404111175504 1106 50-51 0.34396026759100096 1106 52-53 0.23101624707485513 1106 54-55 0.42978967558766357 1106 56-57 0.5255167269650158 1106 58-59 0.3369818437497898 1106 60-61 0.364570650882321 1106 62-63 0.39464136564428287 1106 64-65 0.13556637177843456 1106 66-67 0.0365234266671699 1106 68-69 0.18756791615300727 1106 70-71 -0.12905037828875976 1106 72-73 0.14281677139648252 1106 74-75 0.2602168631721504 1106 76-77 -0.0024027893529741107 1106 78-79 0.42485891832200195 1106 80-81 0.5904593903560134 1106 82-83 0.4841095909933202 1106 84-85 0.8978904959157354 1106 86-87 0.9596709638023277 1106 88-89 0.8732086232684466 1106 90-91 0.8289739986068057 1106 92-93 0.9902061546439569 1106 94-95 1.0178429742127513 1106 96-97 1.1515989048559696 1106 98-99 1.2510512645847456 1106 100-101 1.812916549312451 1107 1 0.1466289268197727 1107 2 0.2129935403077674 1107 3 0.1660324717551518 1107 4 0.25419820879872645 1107 5 0.23176274643149242 1107 6 0.24053126332253782 1107 7 0.39678368434976363 1107 8 0.33010687942503125 1107 9 0.41104103482294363 1107 10-11 0.5561532784722658 1107 12-13 0.24726737437804047 1107 14-15 0.2133893491792236 1107 16-17 0.3545580703457958 1107 18-19 0.48706071682957486 1107 20-21 0.39219119751470544 1107 22-23 0.27885764123846 1107 24-25 0.3301589263951854 1107 26-27 0.2322844627138707 1107 28-29 0.34597094859246624 1107 30-31 0.3723781791717826 1107 32-33 0.16390684995307936 1107 34-35 0.3913734549312835 1107 36-37 0.38011078790670183 1107 38-39 0.45311248123910275 1107 40-41 0.3090350400675561 1107 42-43 0.4043030213500103 1107 44-45 0.44202821012994065 1107 46-47 0.45745429781665337 1107 48-49 0.48036866934381806 1107 50-51 0.4270698559058701 1107 52-53 0.457865478275572 1107 54-55 0.6175747367170814 1107 56-57 0.5713710595521491 1107 58-59 0.7806737733667362 1107 60-61 0.7142982235082442 1107 62-63 0.6665826287290955 1107 64-65 0.7042585151645078 1107 66-67 0.487612623117581 1107 68-69 0.4557707847636614 1107 70-71 0.59697284900939 1107 72-73 0.5971501281019087 1107 74-75 0.38030936534832094 1107 76-77 0.42361043355007766 1107 78-79 0.6484922067923193 1107 80-81 0.5408705111389907 1107 82-83 0.5098679155906183 1107 84-85 0.6349318115022413 1107 86-87 0.6706756273895813 1107 88-89 0.7841665141709449 1107 90-91 0.6783257651093528 1107 92-93 0.8543233822123071 1107 94-95 0.8090375673930055 1107 96-97 0.9127922838275886 1107 98-99 0.9183722072894902 1107 100-101 1.2604725696495898 1108 1 0.3031848190672335 1108 2 0.24346276638942754 1108 3 0.2413782766018464 1108 4 0.34615934175307217 1108 5 0.382152168184021 1108 6 0.3451534031804613 1108 7 0.5506958495013166 1108 8 0.41194137726714075 1108 9 0.14626679926661268 1108 10-11 0.380400104246732 1108 12-13 0.33397569623324586 1108 14-15 0.5879170761749606 1108 16-17 0.7289760246503008 1108 18-19 0.696532889661043 1108 20-21 0.822230932724608 1108 22-23 0.751030472706077 1108 24-25 0.6419275606225554 1108 26-27 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-5.596154588921845 2316 98-99 -6.877914272206347 2316 100-101 -7.770015281821053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1981.0 3 698.0 4 695.0 5 747.0 6 815.0 7 1088.0 8 1483.0 9 1922.0 10 2699.0 11 3913.0 12 5744.0 13 7452.0 14 8321.0 15 11048.0 16 14790.0 17 19660.0 18 14064.0 19 8266.0 20 7597.0 21 8039.0 22 8553.0 23 9596.0 24 10966.0 25 12568.0 26 14488.0 27 17256.0 28 20550.0 29 24770.0 30 29412.0 31 36456.0 32 44914.0 33 57051.0 34 75231.0 35 103640.0 36 136501.0 37 168088.0 38 128800.0 39 19052.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.87688525711357 18.4348452637024 14.03468381816763 19.653585661016393 2 15.71529259518876 21.690924426710627 40.059485753460535 22.53429722464008 3 19.661031634480235 29.30800777865491 29.410054489092552 21.620906097772302 4 12.039168454971778 17.02273915391747 37.48451178548426 33.45358060562649 5 12.26754703938089 39.847070864034215 31.99679199269052 15.888590103894378 6 28.41097829278978 36.65394114163846 18.633923714248578 16.30115685132318 7 27.611475889919003 35.487230172858915 20.28433480529289 16.616959131929192 8 28.03684402637715 31.64612553126532 19.752909258487 20.56412118387053 9 29.542899800130034 16.182242878127482 20.372962169191126 33.901895152551354 10-11 25.042064963521227 27.68255038405047 25.561869446919168 21.713515205509136 12-13 27.955428852610247 25.738523189543894 26.52862443902089 19.777423518824968 14-15 24.312789111519102 25.497715997933394 27.758750445990703 22.430744444556794 16-17 20.757166577417525 29.49656743960273 27.677233379434686 22.069032603545054 18-19 20.657053180434406 26.785241343576676 32.35551600498106 20.202189471007856 20-21 22.992706351712506 24.958313757807744 32.03419234590532 20.014787544574432 22-23 21.913035023388396 25.606755342652754 32.03810686364454 20.442102770314307 24-25 21.555653651137344 25.796222956387954 32.41647126443974 20.231652128034952 26-27 21.194459662037843 25.820041073060175 32.74036068424291 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21.72490364957321 28.184328665647655 28.321298353283613 21.769469331495518 54-55 21.125906475415672 27.812792974201905 28.624650356044867 22.43665019433755 56-57 21.86543739056693 28.086578103086314 27.827259627463118 22.22072487888364 58-59 22.193344566741317 27.454131453975517 28.126499532422628 22.226024446860542 60-61 21.88710011998747 27.879494656875792 28.776681328592872 21.456723894543867 62-63 22.04044544081565 28.04917021295028 28.68961892651805 21.22076541971602 64-65 21.63682207160489 28.305545381960606 28.61067301819841 21.4469595282361 66-67 21.802354961387525 28.326048475666394 28.018563633335997 21.853032929610084 68-69 21.667605172853587 28.324886777651038 28.425619775047284 21.581888274448087 70-71 21.872640213150266 28.27667759337717 28.1883141791034 21.662368014369164 72-73 21.706398815039602 28.25270811300255 28.1370810867015 21.90381198525635 74-75 21.20605627396192 29.309198730213744 27.827861652021767 21.656883343802573 76-77 21.212822885245462 28.721514514611858 28.204108630203063 21.861553969939624 78-79 21.13839140658658 28.719339753201712 28.2139977520031 21.928271088208607 80-81 21.20288548502073 29.335363267970145 27.678380185322187 21.78337106168694 82-83 21.35217956930819 28.904278569802482 27.577395875396526 22.1661459854928 84-85 21.096168109014236 28.73290155153635 27.602350045506018 22.56858029394339 86-87 21.312204432987805 28.925885533928902 27.379142991626665 22.382767041456628 88-89 21.243502119797302 28.56911155249825 27.39411481316955 22.793271514534894 90-91 21.10727726115192 28.33539732327858 27.742960815636202 22.814364599933292 92-93 21.30338601581437 28.745227675726458 27.72896086068375 22.222425447775418 94-95 20.865266224283214 28.406707182650198 27.982550466081424 22.745476126985167 96-97 20.778533788480015 28.401037364770033 28.100927428748555 22.719501418001396 98-99 20.866402323345763 28.672457059454143 27.451044576525174 23.010096040674917 100-101 20.848396217968403 28.48437615829772 27.927382374716114 22.739845249017762 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 934.0 1 708.0 2 1110.5 3 1551.0 4 1177.5 5 1153.0 6 1674.0 7 2880.0 8 4296.5 9 4831.5 10 4135.5 11 3301.0 12 3162.5 13 3299.5 14 3408.5 15 3361.0 16 3288.5 17 3292.0 18 3351.5 19 3405.5 20 3590.5 21 3915.5 22 4246.5 23 4674.5 24 5162.0 25 5773.0 26 6665.5 27 7806.5 28 8850.5 29 10277.0 30 12429.5 31 14751.5 32 17750.0 33 20454.5 34 22481.0 35 24298.5 36 27012.0 37 29591.5 38 29243.5 39 28283.0 40 27889.5 41 28273.5 42 28403.0 43 29204.0 44 30717.5 45 31671.5 46 31865.0 47 34664.5 48 45019.5 49 45189.5 50 36272.0 51 31846.0 52 28606.5 53 27559.5 54 27275.5 55 26081.0 56 24334.5 57 22754.5 58 22091.5 59 21158.0 60 19318.0 61 18099.0 62 16093.0 63 11668.0 64 7745.5 65 5811.0 66 4242.0 67 3224.5 68 2439.0 69 1654.5 70 1272.0 71 1052.0 72 933.5 73 942.5 74 770.0 75 583.5 76 413.0 77 238.0 78 139.5 79 96.5 80 68.5 81 48.0 82 25.0 83 14.5 84 10.5 85 7.5 86 3.5 87 1.0 88 1.5 89 2.5 90 1.5 91 1.0 92 1.5 93 1.0 94 2.0 95 2.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07652206723161449 2 0.03349645207119729 3 0.01694073438083541 4 0.02146468049389941 5 0.025507355743871495 6 0.030897589410500945 7 0.08123852168991526 8 0.05707872436270112 9 0.07132434191022181 10-11 0.0616989246483835 12-13 0.042496217211016096 14-15 0.047886450877645544 16-17 0.060062603713871 18-19 0.05611618263651729 20-21 0.019635851214150134 22-23 0.0147268884106126 24-25 0.023726653550431413 26-27 0.026854914160528855 28-29 0.00640090247912247 30-31 0.0011550500714205961 32-33 0.01025106938385779 34-35 0.012513042440389793 36-37 0.0049570898898467245 38-39 0.010154815211239407 40-41 0.004379564854136426 42-43 0.002213845970222809 44-45 3.8501669047353204E-4 46-47 7.700333809470641E-4 48-49 0.001636320934512511 50-51 0.0011069229851114045 52-53 0.0 54-55 1.9250834523676602E-4 56-57 0.0037539127321169377 58-59 0.004860835717228342 60-61 0.005534614925557023 62-63 0.003368896041643405 64-65 6.256521220194895E-4 66-67 2.8876251785514903E-4 68-69 0.002983879351169873 70-71 0.0023582272291503836 72-73 0.022282840961155667 74-75 0.015256286360013705 76-77 0.02627738912481856 78-79 0.020550265854024773 80-81 0.012561169526698982 82-83 0.005053344062465107 84-85 0.004090802336281278 86-87 0.002213845970222809 88-89 0.0018769563660584688 90-91 0.006785919169596003 92-93 0.010972975678495664 94-95 0.014341871720139067 96-97 0.012513042440389793 98-99 0.023245382687339496 100-101 0.02069464711295235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1038916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.87428975984096 #Duplication Level Percentage of deduplicated Percentage of total 1 76.63111983749847 36.68660435721499 2 12.594800621468716 12.059342688392368 3 4.280956988702346 6.14843325979657 4 1.965043446101728 3.763002373174022 5 1.0840615850719728 2.5949339220624057 6 0.6673616280865363 1.9169678374568437 7 0.44809813682295113 1.501666603017478 8 0.3220569002722907 1.2334596290233482 9 0.2505898604076915 1.0797130429232327 >10 1.5185056330184097 14.10999740103919 >50 0.14488239500998137 4.848912779756643 >100 0.08481272032793728 7.711748455690946 >500 0.004463827332707338 1.5575031353501998 >1k 0.0026377161511452456 2.404542385709046 >5k 4.058024847915762E-4 1.3377780454061965 >10k+ 2.029012423957881E-4 1.045394083986457 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10762 1.035887405719038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8435 0.8119039460360608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5337 0.51370851926431 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3997 0.3847279279556769 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2414 0.23235757270077656 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2405 0.23149128514721112 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2241 0.21570560083779633 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.1981873414212506 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1863 0.17932152358804754 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1705 0.16411336431434304 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1673 0.1610332307905548 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1432 0.13783597518952445 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1374 0.13225323317765825 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1362 0.13109818310623766 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1210 0.11646754886824344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.8876251785514903E-4 0.0 14-15 0.0 0.0 0.0 2.8876251785514903E-4 0.0 16-17 0.0 0.0 0.0 2.8876251785514903E-4 0.0 18-19 0.0 0.0 0.0 3.8501669047353204E-4 0.0 20-21 0.0 0.0 0.0 3.8501669047353204E-4 0.0 22-23 0.0 0.0 0.0 7.700333809470641E-4 0.0 24-25 0.0 0.0 0.0 0.0010587958988022132 0.0 26-27 0.0 0.0 0.0 0.0011550500714205961 0.0 28-29 0.0 0.0 0.0 0.0019250834523676602 0.0 30-31 0.0 0.0 0.0 0.003946421077353704 0.0 32-33 0.0 0.0 0.0 0.006545283738050044 0.0 34-35 0.0 0.0 0.0 0.008614748449345278 0.0 36-37 0.0 0.0 0.0 0.01155050071420596 0.0 38-39 0.0 0.0 0.0 0.014245617547520685 0.0 40-41 0.0 0.0 0.0 0.019491469955222558 0.0 42-43 0.0 0.0 0.0 0.02728805793731158 0.0 44-45 0.0 0.0 0.0 0.03734661897593261 0.0 46-47 0.0 0.0 0.0 0.06160267047576512 0.0 48-49 0.0 0.0 0.0 0.10891159631770037 0.0 50-51 0.0 0.0 0.0 0.14726888410612599 0.0 52-53 0.0 0.0 0.0 0.1824979112844542 0.0 54-55 0.0 0.0 0.0 0.2264860681710552 0.0 56-57 0.0 0.0 0.0 0.2638326871469878 0.0 58-59 0.0 0.0 0.0 0.31479927154842163 0.0 60-61 0.0 0.0 0.0 0.40022484974723654 0.0 62-63 0.0 0.0 0.0 0.4678915330979598 0.0 64-65 0.0 0.0 0.0 0.5413334668057861 0.0 66-67 0.0 0.0 0.0 0.6616030554924557 0.0 68-69 0.0 0.0 0.0 0.802663545464696 0.0 70-71 0.0 0.0 0.0 0.9271683177465744 0.0 72-73 0.0 0.0 0.0 1.0629348282248037 0.0 74-75 0.0 0.0 0.0 1.1769478956912782 0.0 76-77 0.0 0.0 0.0 1.2712769848572936 0.0 78-79 0.0 0.0 0.0 1.387503898293991 0.0 80-81 0.0 0.0 0.0 1.597337994602066 0.0 82-83 0.0 0.0 0.0 1.8301287110796254 0.0 84-85 0.0 0.0 0.0 2.0768281555005412 0.0 86-87 0.0 0.0 0.0 2.4572727727747 0.0 88-89 0.0 0.0 0.0 2.8967211978639273 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 45 1.5074016E-5 52.827736 1 TATCACG 85 1.2732926E-10 50.336876 2 ATCACGC 90 2.237357E-10 47.547253 3 CGTATCA 60 8.2487946E-5 39.61698 2 TACCTGG 985 0.0 36.680904 2 TATAACG 80 1.0712505E-5 35.65528 2 AGGTGCG 70 2.0400825E-4 33.965595 9 TCACGCA 130 8.201823E-9 32.918915 4 TAGCGCG 95 3.4657696E-5 30.031294 4 CGAATAC 65 0.0058141206 29.261257 4 AGCGCGC 115 3.8328562E-6 28.943201 5 CGCGCGT 100 4.917581E-5 28.529726 7 GTACCTG 1275 0.0 28.340525 1 GCGCGCG 105 6.856367E-5 27.171167 6 GTACATG 12530 0.0 27.130604 1 TACATGG 12690 0.0 26.298933 2 ACATGGG 12650 0.0 25.183132 3 ACCTGGG 1405 0.0 25.04268 3 GTATCAA 17490 0.0 24.574411 1 GAGTACT 8085 0.0 23.81601 12-13 >>END_MODULE