FastQCFastQC Report
Thu 26 May 2016
SRR936488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038916
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130201.2532293274913466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113481.0922923508734104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73650.7089119813343908No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47740.4595174200801605No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.35989435142013404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28470.2740356294445364No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.2660465331172106No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22250.2141655340759022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.20925657127236463No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.17181369812381367No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17560.16902232711788057No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16030.15429543870726797No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15380.14803891748707307No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14550.1400498211597473No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13380.12878808296339644No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12220.11762259893966402No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12040.11589002383253315No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.11232861944565296No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11150.10732340246949706No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.10530206484451102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCC405.392153E-447.492478
TACCTGG9050.029.9224642
GTACATG113800.025.0556431
GTATCAA187900.024.7348141
TACATGG115450.024.6081142
GAGTACT109800.023.87714612-13
GGTATCA138950.023.8038521
GTACCTG11600.023.7611031
ACATGGG115550.023.5955123
ATGGGAG25350.022.1069455
AGTACTT111700.022.0465812-13
GTACTTT120550.021.7665614-15
CATGGGA88300.021.7292824
CGTCACT2858.367351E-1121.6767941
CATGGGG25000.021.6565654
AAGTTCG1100.002879830521.5874849
ATGGGAT30200.021.5445985
TCAACGC225250.020.6204814
ATCAACG225150.020.5904243
CAACGCA227900.020.4015465
Produced by FastQC (version 0.11.5)