##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936488_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1038916 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781002506458655 31.0 31.0 34.0 30.0 34.0 2 31.89286910587574 31.0 31.0 34.0 30.0 34.0 3 31.940044238417734 31.0 31.0 34.0 30.0 34.0 4 35.709705115716766 37.0 35.0 37.0 35.0 37.0 5 35.5552662582923 37.0 35.0 37.0 33.0 37.0 6 35.679536170392986 37.0 35.0 37.0 33.0 37.0 7 35.613398003303445 37.0 35.0 37.0 33.0 37.0 8 35.599762637210326 37.0 35.0 37.0 33.0 37.0 9 37.17386679962576 39.0 37.0 39.0 33.0 39.0 10-11 37.060105436820685 39.0 37.0 39.0 33.0 39.0 12-13 36.94652118169323 39.0 37.0 39.0 33.0 39.0 14-15 37.91987850798332 40.0 37.0 41.0 33.0 41.0 16-17 37.86466759584028 39.0 37.0 41.0 33.0 41.0 18-19 38.11908277473829 40.0 37.0 41.0 33.0 41.0 20-21 38.1708997647548 40.0 37.0 41.0 33.0 41.0 22-23 38.05864285466775 40.0 37.0 41.0 33.0 41.0 24-25 37.896772212575414 40.0 37.0 41.0 33.0 41.0 26-27 37.65684473046907 39.5 36.5 41.0 32.5 41.0 28-29 37.50634796268419 39.0 36.0 41.0 32.0 41.0 30-31 37.3064684729083 39.0 36.0 41.0 32.0 41.0 32-33 36.96750651640748 39.0 35.0 41.0 31.5 41.0 34-35 36.687977180060756 39.0 35.0 41.0 31.0 41.0 36-37 36.378997917059706 38.5 35.0 40.5 30.5 41.0 38-39 36.13409746312502 38.0 35.0 40.0 30.0 41.0 40-41 35.84476945200574 38.0 35.0 40.0 30.0 41.0 42-43 35.392992792487554 38.0 35.0 40.0 27.5 41.0 44-45 34.87949699494473 38.0 34.5 40.0 25.0 41.0 46-47 34.16645859723019 38.0 33.5 40.0 22.0 41.0 48-49 33.51636946586635 38.0 33.0 40.0 16.5 41.0 50-51 33.13664916124114 38.0 33.0 40.0 10.5 41.0 52-53 32.74435565531766 37.0 32.5 40.0 8.0 41.0 54-55 32.53001156975155 37.0 32.0 40.0 6.5 41.0 56-57 32.21512085673914 36.5 31.5 40.0 2.0 41.0 58-59 31.854198992026305 36.0 31.0 39.0 2.0 41.0 60-61 31.43252678753624 35.5 31.0 39.0 2.0 40.0 62-63 31.100383957894575 35.0 30.0 39.0 2.0 40.0 64-65 30.666885484485753 35.0 29.5 38.0 2.0 40.0 66-67 30.249692949189345 35.0 29.0 38.0 2.0 40.0 68-69 29.903981650104534 34.5 29.0 37.0 2.0 40.0 70-71 29.544612846466897 34.0 29.0 37.0 2.0 39.0 72-73 29.09987236696711 34.0 28.0 36.0 2.0 39.0 74-75 28.622366004566295 34.0 27.5 36.0 2.0 38.5 76-77 27.171038852034236 31.5 25.5 34.5 2.0 36.5 78-79 27.89777084961633 33.0 26.5 35.0 2.0 37.0 80-81 27.67561718175483 33.0 26.0 35.0 2.0 37.0 82-83 27.374421031151698 33.0 26.0 35.0 2.0 36.0 84-85 26.991350118777646 33.0 25.5 35.0 2.0 36.0 86-87 26.60748703456295 33.0 25.0 35.0 2.0 35.5 88-89 26.12780051515233 32.5 24.0 35.0 2.0 35.0 90-91 25.628407878981555 32.0 22.0 34.5 2.0 35.0 92-93 25.026755772362733 32.0 19.0 34.0 2.0 35.0 94-95 24.495482791679017 31.5 14.5 34.0 2.0 35.0 96-97 23.78479973356845 31.0 2.0 34.0 2.0 35.0 98-99 22.950493110126324 31.0 2.0 34.0 2.0 35.0 100-101 19.97322882696965 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.424318332893332 1101 2 0.39768566247300186 1101 3 0.3945117912972016 1101 4 0.34117702433049146 1101 5 0.20824269369649073 1101 6 0.3085296583486894 1101 7 0.2571572975533556 1101 8 0.2914778425581943 1101 9 0.4722966022195223 1101 10-11 0.45601680220390506 1101 12-13 0.41249373250083465 1101 14-15 0.5718591061065652 1101 16-17 0.6921856721667723 1101 18-19 0.5227384008297449 1101 20-21 0.4434781301438093 1101 22-23 0.5447061116185523 1101 24-25 0.48216704488920925 1101 26-27 0.40846616739863606 1101 28-29 0.3985342202005455 1101 30-31 0.4454848460111478 1101 32-33 0.6487536201309183 1101 34-35 0.38421686387420095 1101 36-37 0.5663094491966802 1101 38-39 0.5001708153649034 1101 40-41 0.5381286565902599 1101 42-43 0.7472688134936405 1101 44-45 0.7095944213877488 1101 46-47 0.6201162940804821 1101 48-49 0.4969238101476421 1101 50-51 0.5562384109852943 1101 52-53 0.6916912668626409 1101 54-55 0.7453129733931974 1101 56-57 0.6707203716660004 1101 58-59 0.6139705999473364 1101 60-61 0.553086567194935 1101 62-63 0.5135202248898878 1101 64-65 0.6131354665705935 1101 66-67 0.5702322738340371 1101 68-69 0.3580306836471827 1101 70-71 0.33327143694694783 1101 72-73 0.2032373405017971 1101 74-75 0.39732265391443633 1101 76-77 0.434300249020211 1101 78-79 0.4905533837472227 1101 80-81 0.5945074037176923 1101 82-83 0.5676792078561199 1101 84-85 0.31756618845489726 1101 86-87 0.31624994163913556 1101 88-89 0.2303883186075133 1101 90-91 0.2907277942576876 1101 92-93 0.24901477768388958 1101 94-95 0.21882364207459304 1101 96-97 0.18634397359925714 1101 98-99 0.1833798425850226 1101 100-101 0.6889655890119535 1102 1 0.22865716687251947 1102 2 0.20509411808322398 1102 3 0.18786856405811747 1102 4 0.12804840500711379 1102 5 0.05406931450025354 1102 6 0.1201974861015529 1102 7 0.08585499424861354 1102 8 0.19857824748700637 1102 9 0.19319833078660054 1102 10-11 0.38056180155079744 1102 12-13 0.29741176707217676 1102 14-15 0.5502743323006669 1102 16-17 0.392273842149784 1102 18-19 0.16176069368471246 1102 20-21 0.3584877091049279 1102 22-23 0.41340195783268996 1102 24-25 0.3563615413406396 1102 26-27 0.4573934326052367 1102 28-29 0.44168471874193216 1102 30-31 0.4577246901353291 1102 32-33 0.47257856461648373 1102 34-35 0.5181819006661144 1102 36-37 0.46993312664693576 1102 38-39 0.3391065710143195 1102 40-41 0.5093487767626854 1102 42-43 0.4627540749557397 1102 44-45 0.5730719108291211 1102 46-47 0.4036323170699987 1102 48-49 0.404262600586101 1102 50-51 0.2647702174902733 1102 52-53 0.6649148049825513 1102 54-55 0.45146963294821063 1102 56-57 0.22293931623865149 1102 58-59 0.3843912034218917 1102 60-61 0.3693027687014414 1102 62-63 0.24701665454983868 1102 64-65 0.20731134935894246 1102 66-67 0.13745970884949088 1102 68-69 0.10665860631884172 1102 70-71 0.2811243345826817 1102 72-73 0.3239883092830915 1102 74-75 0.25066222808607463 1102 76-77 0.13467306654100142 1102 78-79 0.3305139793844418 1102 80-81 0.2567216676616155 1102 82-83 0.23588413231295036 1102 84-85 0.06856708103990883 1102 86-87 0.22307277439917783 1102 88-89 0.2823280582339329 1102 90-91 0.15330235776184864 1102 92-93 0.10994350150502541 1102 94-95 -0.042047934535322185 1102 96-97 0.3889014473659671 1102 98-99 0.5660566179583846 1102 100-101 0.8285751374945569 1103 1 0.2766940572498733 1103 2 0.3519583342135988 1103 3 0.27035377838673114 1103 4 0.29756498226415573 1103 5 0.3225465684880575 1103 6 0.36385109930228055 1103 7 0.20862747304988005 1103 8 0.31099006788054595 1103 9 0.28635365922700373 1103 10-11 0.3257667650574163 1103 12-13 0.26699196597890307 1103 14-15 0.4322388670482624 1103 16-17 0.4965619413384914 1103 18-19 0.3126605582978357 1103 20-21 0.35635103962193426 1103 22-23 0.2253195652514819 1103 24-25 0.18954557586938137 1103 26-27 0.14030669339292245 1103 28-29 0.14734450069781957 1103 30-31 0.37850250297569943 1103 32-33 0.2561595982392504 1103 34-35 0.45575481107665894 1103 36-37 0.2638152688133246 1103 38-39 0.3815610433618062 1103 40-41 0.47159619881343673 1103 42-43 0.5317448828997939 1103 44-45 0.5108926911868252 1103 46-47 0.5623586625406389 1103 48-49 0.3804365472660649 1103 50-51 0.34110245483466173 1103 52-53 0.48824613635464686 1103 54-55 0.4117870286341514 1103 56-57 0.34763774961914606 1103 58-59 0.5033153358770761 1103 60-61 0.34315834246692845 1103 62-63 0.296494957022432 1103 64-65 0.5184975622929677 1103 66-67 0.17592332235560804 1103 68-69 0.3977475035775093 1103 70-71 0.35248339481457336 1103 72-73 0.6358071759015722 1103 74-75 0.3726284933425461 1103 76-77 0.2930538192105665 1103 78-79 0.26655386252419433 1103 80-81 0.37688056909386347 1103 82-83 0.2721881725421902 1103 84-85 0.5128772696653741 1103 86-87 0.7288648893864078 1103 88-89 0.4919904054147288 1103 90-91 0.48185473619360053 1103 92-93 0.8760021148584443 1103 94-95 0.8442270942216616 1103 96-97 0.7216609982480193 1103 98-99 0.5707671649009818 1103 100-101 0.8928141716670233 1104 1 0.2690375008708976 1104 2 0.3963265349228564 1104 3 0.35396516527530864 1104 4 0.3197743313425292 1104 5 0.33102061184726495 1104 6 0.2780511720948269 1104 7 0.18954324902414754 1104 8 0.29556643240732683 1104 9 0.28061886419899906 1104 10-11 0.30043133814437795 1104 12-13 0.30029693816719316 1104 14-15 0.3454161283334898 1104 16-17 0.45061450441742323 1104 18-19 0.46774060151602725 1104 20-21 0.4078708222351892 1104 22-23 0.3833637133595431 1104 24-25 0.33687997758895705 1104 26-27 0.3475820416823936 1104 28-29 0.4183490569014765 1104 30-31 0.3941981577179874 1104 32-33 0.5472311731307755 1104 34-35 0.3304844591761409 1104 36-37 0.6051627693802288 1104 38-39 0.448597834087046 1104 40-41 0.5827429863182729 1104 42-43 0.456812863897504 1104 44-45 0.6053048151475764 1104 46-47 0.5462032904697338 1104 48-49 0.49255707800377735 1104 50-51 0.34695110731503576 1104 52-53 0.4798130626226538 1104 54-55 0.3291265182097689 1104 56-57 0.13046156688317723 1104 58-59 0.05413434730855471 1104 60-61 0.16408724777754458 1104 62-63 0.19867282618259097 1104 64-65 0.12238700908866917 1104 66-67 0.18562311979414403 1104 68-69 0.1993087322089906 1104 70-71 0.18558551970905057 1104 72-73 0.447544592985718 1104 74-75 0.3079279241505617 1104 76-77 0.09974244432704538 1104 78-79 0.043929195759709216 1104 80-81 0.15075633393963628 1104 82-83 0.1462513496129212 1104 84-85 0.2041253950667361 1104 86-87 0.21986395756558252 1104 88-89 0.20219508205003578 1104 90-91 0.43423087929383897 1104 92-93 0.3911770742222913 1104 94-95 0.11690283010286251 1104 96-97 0.22409440354815047 1104 98-99 0.43085160044432413 1104 100-101 0.9239229812802101 1105 1 0.233499960089663 1105 2 0.21605050841137796 1105 3 0.29102153155693955 1105 4 0.2916003754891321 1105 5 0.23644488366338834 1105 6 0.31049521729954677 1105 7 0.2434700044543021 1105 8 0.08391640889382046 1105 9 0.22453272394093915 1105 10-11 0.3542056231868429 1105 12-13 0.13414830342011186 1105 14-15 0.41926966123769915 1105 16-17 0.26053858151232845 1105 18-19 0.3392650114739979 1105 20-21 0.2104042187610773 1105 22-23 0.19795604795672972 1105 24-25 0.18035029546216919 1105 26-27 0.031779128946894275 1105 28-29 0.1680624860523494 1105 30-31 0.312901130706841 1105 32-33 0.3106177976200968 1105 34-35 0.33564686769390306 1105 36-37 0.3588992123746806 1105 38-39 0.21852690780188055 1105 40-41 0.4328439290628907 1105 42-43 0.339642126455189 1105 44-45 0.5107745525311174 1105 46-47 0.35993040154165357 1105 48-49 0.38390814933787 1105 50-51 0.45667926914673984 1105 52-53 0.4519050114513874 1105 54-55 0.3722347648370459 1105 56-57 0.2660141710039241 1105 58-59 0.27840910262113994 1105 60-61 0.17684516154176322 1105 62-63 0.09267972960086723 1105 64-65 0.27036824604602216 1105 66-67 0.2619896586061259 1105 68-69 0.04212875469378119 1105 70-71 0.3010028476930593 1105 72-73 0.25077283197110845 1105 74-75 0.1693695408510294 1105 76-77 0.13541592256235546 1105 78-79 0.09329561700416278 1105 80-81 -0.08466483335895703 1105 82-83 0.04025687528672961 1105 84-85 0.26427725924684964 1105 86-87 0.3722842871955372 1105 88-89 0.3118038393612679 1105 90-91 0.15206835320854495 1105 92-93 0.16887249349748856 1105 94-95 0.3860314342823905 1105 96-97 0.42852550530284717 1105 98-99 0.0837252080039157 1105 100-101 0.4449179699643331 1106 1 0.23131812064753277 1106 2 0.18191619285165928 1106 3 0.12257874305451466 1106 4 0.2111283257116554 1106 5 0.324242882359421 1106 6 0.17039596707985538 1106 7 0.2124795574208207 1106 8 0.21118506127356085 1106 9 0.1963672407649355 1106 10-11 0.10725470615875565 1106 12-13 0.2852843127179625 1106 14-15 0.24541966690711803 1106 16-17 0.1910310943393796 1106 18-19 0.22096909628847072 1106 20-21 0.17206390495887547 1106 22-23 0.11498059652945614 1106 24-25 0.010585381353827472 1106 26-27 0.13665447265525188 1106 28-29 -0.20973324487715672 1106 30-31 0.09965046455042881 1106 32-33 0.15896844039016855 1106 34-35 0.22190287373184248 1106 36-37 0.16965055177428923 1106 38-39 0.43904549994406494 1106 40-41 0.6148795861482412 1106 42-43 0.6111504746707439 1106 44-45 0.4156767373824337 1106 46-47 0.35242373997746057 1106 48-49 0.5373703910250853 1106 50-51 0.7597919558831379 1106 52-53 0.31278240677978886 1106 54-55 0.5502433646329479 1106 56-57 0.33397718514911645 1106 58-59 0.5219247076895925 1106 60-61 0.25312184323238185 1106 62-63 0.432993088871779 1106 64-65 0.40304897640858073 1106 66-67 0.5317573188380038 1106 68-69 0.3218599712087915 1106 70-71 0.5284093377574095 1106 72-73 0.7871682702169913 1106 74-75 0.4971862663378914 1106 76-77 0.38213740539819696 1106 78-79 0.2740498974134944 1106 80-81 0.5110938231311088 1106 82-83 0.5034694500521582 1106 84-85 0.8970261997746718 1106 86-87 0.7828133677579459 1106 88-89 0.7954927514002748 1106 90-91 0.7102414369458216 1106 92-93 0.7674113786067629 1106 94-95 0.7303581202250697 1106 96-97 0.9517430939583456 1106 98-99 1.034219687409763 1106 100-101 1.815727916955293 1107 1 0.04065572708318399 1107 2 0.11792055716213667 1107 3 0.10092991613397473 1107 4 0.1708764543767316 1107 5 0.0704014511665747 1107 6 -0.038971225002818244 1107 7 0.06931067810118918 1107 8 0.059089183255352395 1107 9 0.02258234723051089 1107 10-11 0.059827887881318986 1107 12-13 -0.041569108357627726 1107 14-15 0.004093562960598263 1107 16-17 0.10672525399584742 1107 18-19 0.051864036458766805 1107 20-21 0.04625991927627382 1107 22-23 -0.05922499464479358 1107 24-25 0.02782671643607415 1107 26-27 0.15199753789930526 1107 28-29 0.0963905565193528 1107 30-31 0.14536712982046396 1107 32-33 0.3663202171536426 1107 34-35 0.15944039021344025 1107 36-37 0.2974690976995902 1107 38-39 0.11252256593716226 1107 40-41 0.07000132339007337 1107 42-43 0.15311506424257004 1107 44-45 0.11075602862662492 1107 46-47 -0.10997085046654576 1107 48-49 0.06081316845038032 1107 50-51 0.10033301409161055 1107 52-53 0.1991671091191023 1107 54-55 0.22208466927448 1107 56-57 0.22708107865447147 1107 58-59 0.24600092578883448 1107 60-61 0.3447548341360793 1107 62-63 0.245345267521202 1107 64-65 0.20126802306668523 1107 66-67 0.28951756564728726 1107 68-69 0.2889113082919188 1107 70-71 0.3010876767884021 1107 72-73 0.23389443880729743 1107 74-75 0.4249293090079185 1107 76-77 0.20125195657709227 1107 78-79 0.14733867556047286 1107 80-81 0.46848578849024847 1107 82-83 0.5703244615700385 1107 84-85 0.7221613446160831 1107 86-87 0.5582997780270702 1107 88-89 0.5024529031250502 1107 90-91 0.6408960151109291 1107 92-93 0.7421284765455205 1107 94-95 0.6635056739367009 1107 96-97 1.1068509789608143 1107 98-99 1.0025472261746593 1107 100-101 1.2699103526578135 1108 1 0.10199570521212209 1108 2 0.06767722074014415 1108 3 0.1246324697055492 1108 4 0.006601413264142764 1108 5 0.12737473007268818 1108 6 0.05542828081137685 1108 7 0.12987515181835363 1108 8 0.13428222458497885 1108 9 0.22514760563458935 1108 10-11 0.291217868931966 1108 12-13 0.22838865211251402 1108 14-15 0.20919027144940827 1108 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-1.262544845759411 2316 96-97 -1.670432828030485 2316 98-99 -2.1215059832186505 2316 100-101 -3.169436784476627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 26.0 5 62.0 6 152.0 7 356.0 8 775.0 9 1472.0 10 2444.0 11 4097.0 12 5907.0 13 8134.0 14 9744.0 15 13367.0 16 17625.0 17 22703.0 18 18374.0 19 9768.0 20 8181.0 21 8318.0 22 8613.0 23 9484.0 24 10981.0 25 12785.0 26 15388.0 27 18650.0 28 22999.0 29 27517.0 30 33489.0 31 39855.0 32 49132.0 33 61506.0 34 80183.0 35 105997.0 36 139165.0 37 169954.0 38 96517.0 39 5184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.392257783759796 19.255508732810934 15.06976717318029 19.28246631024898 2 16.02154046683205 22.49484736553814 39.08435688094502 22.399255286684784 3 19.67332259682352 28.953633102078026 30.342031318136005 21.031012982962448 4 12.212055642612107 17.699987294449215 37.01396455536348 33.073992507575205 5 11.992611223893585 39.90468517325987 32.337157669898055 15.765545932948493 6 28.434830149887 37.19655872082055 17.82935290244832 16.539258226844133 7 26.808930470731486 35.804854102597425 20.422652478787967 16.96356294788313 8 28.024979882877922 31.781587731828175 19.608515029126515 20.58491735616739 9 28.777302496063207 15.996095930758598 20.549495820643827 34.67710575253437 10-11 25.046250129943132 27.709554959207484 25.523670826130314 21.720524084719074 12-13 27.97145649917148 25.712492618433537 26.92824323290232 19.387807649492665 14-15 24.019890000519773 25.646636776480054 28.455868339439068 21.8776048835611 16-17 20.1540355524412 29.309491816470246 28.720320025873118 21.816152605215436 18-19 20.084801232072383 26.030898065261333 34.055443257291365 19.828857445374915 20-21 22.061311982874457 24.158594150056405 34.19188846836511 19.588205398704034 22-23 21.19575653650369 24.723521787864364 34.19450378784415 19.886217887787794 24-25 20.976334949120044 24.905670910833987 34.60597391896938 19.512020221076583 26-27 20.632238453127165 24.86384585175032 34.86128544146864 19.642630253653877 28-29 20.067736147635348 25.295557540591634 34.90688084521467 19.729825466558346 30-31 20.184050083646003 25.118586738691427 34.671797724905716 20.02556545275685 32-33 19.845135554520215 25.404632456846265 34.454691946047724 20.295540042585795 34-35 20.670770708671572 24.931694219191648 34.4575892932343 19.939945778902477 36-37 20.927586674893227 24.63627779472929 34.120198939081405 20.315936591296076 38-39 20.65880194551743 25.07784668606537 34.238199789970366 20.025151578446838 40-41 20.384988555356845 25.410139819080307 33.98803943348535 20.216832192077497 42-43 20.328204496351546 25.742914499436644 32.89918945155985 21.029691552651958 44-45 20.648012944472946 25.634638208334014 32.5098974972543 21.207451349938733 46-47 21.360017633815342 26.235546191067012 31.504851705580737 20.89958446953691 48-49 21.49619384556595 26.59654185470438 30.504293203594727 21.402971096134937 50-51 21.37803447733187 26.998612494098406 30.18390830044243 21.43944472812729 52-53 21.62250352050653 27.46567806928973 29.396686729302644 21.515131680901096 54-55 21.055405262972982 26.963965234365787 29.770011439990107 22.210618062671124 56-57 21.789859108016223 27.294951888018044 28.844855163464256 22.070333840501473 58-59 22.158941296364137 26.60940735067512 29.156856411562327 22.07479494139842 60-61 21.813235161035045 27.124205029661564 29.776127880714498 21.286431928588893 62-63 22.040203757568346 27.11354194593965 29.78666873318646 21.05958556330554 64-65 21.688672388314746 27.365282143355685 29.691021376757533 21.255024091572043 66-67 21.788319569464186 27.474187631720927 29.130135341371066 21.607357457443825 68-69 21.887806396996083 27.37663867738239 29.36261531923618 21.372939606385348 70-71 22.02232904296401 27.468967654747832 29.145137816724354 21.3635654855638 72-73 21.8421412318224 27.44461534907538 29.119486079721558 21.593757339380662 74-75 21.36697838925522 28.454395952659752 28.677371211694624 21.501254446390405 76-77 21.286332935155393 27.95372793697944 29.113609696418997 21.646329431446176 78-79 21.13329145940274 27.7996693403412 29.225837659102886 21.84120154115317 80-81 21.28024267302294 28.314853036878446 28.629024494495383 21.775879795603238 82-83 21.3160158323919 27.94004177109842 28.620952071058035 22.12299032545163 84-85 21.01944526149022 27.730859826878294 28.430432871652993 22.81926203997849 86-87 21.178250904158695 27.89323563189069 28.197910880805072 22.730602583145536 88-89 21.148045562850648 27.562067805047853 28.27423409144635 23.01565254065515 90-91 20.92867838588686 27.253109156302813 28.744396777936664 23.07381567987366 92-93 21.097571895258078 27.641053735260616 28.60386143656686 22.657512932914447 94-95 20.700115057071194 27.247440100518478 28.77702517294666 23.27541966946366 96-97 20.52225529003403 27.18846614411927 29.054729769745634 23.234548796101066 98-99 20.62277212514764 27.26656397717462 28.420015109704256 23.690648787973483 100-101 20.468280346055355 27.18473203631597 28.75937441913995 23.58761319848873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1614.0 1 1236.5 2 1833.0 3 2416.0 4 1609.5 5 1280.5 6 1790.0 7 3224.5 8 4746.5 9 5318.5 10 4614.0 11 3641.5 12 3362.0 13 3312.5 14 3396.5 15 3589.0 16 3632.0 17 3628.0 18 3786.5 19 3914.5 20 4038.5 21 4274.5 22 4648.5 23 5055.5 24 5475.0 25 6076.5 26 6974.5 27 7981.0 28 9040.0 29 10576.0 30 12653.0 31 14712.0 32 17614.0 33 20507.5 34 22415.0 35 24002.0 36 26658.5 37 29317.5 38 29107.5 39 27957.5 40 27753.0 41 28287.0 42 28381.5 43 29450.0 44 30905.0 45 31485.5 46 31378.0 47 34354.0 48 45310.0 49 45157.5 50 35543.0 51 30957.0 52 27628.5 53 27042.5 54 26864.5 55 25483.5 56 23957.5 57 22374.0 58 21553.0 59 20715.5 60 18884.5 61 17250.5 62 15082.0 63 11244.5 64 7625.5 65 5691.0 66 4280.0 67 3222.0 68 2345.5 69 1619.0 70 1209.0 71 1014.0 72 950.5 73 920.5 74 782.5 75 649.0 76 483.5 77 285.0 78 180.0 79 130.5 80 86.0 81 58.5 82 51.5 83 36.5 84 18.5 85 7.0 86 2.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023774780636740604 2 0.012224279922534642 3 0.00895163805350962 4 0.0 5 0.004138929422590469 6 0.0 7 9.625417261838301E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0010106688124930216 14-15 1.9250834523676602E-4 16-17 0.0 18-19 0.0015400667618941282 20-21 0.0 22-23 4.8127086309191505E-5 24-25 0.0 26-27 0.00192508345236766 28-29 0.0030801335237882563 30-31 0.001347558416657362 32-33 0.006930300428523576 34-35 0.0020694647112952345 36-37 4.81270863091915E-4 38-39 0.001443812589275745 40-41 1.9250834523676602E-4 42-43 0.0049570898898467245 44-45 0.001636320934512511 46-47 2.8876251785514903E-4 48-49 6.256521220194895E-4 50-51 1.4438125892757452E-4 52-53 2.8876251785514903E-4 54-55 0.0015881938482033198 56-57 0.013619965425501195 58-59 0.02401541606828656 60-61 0.019058326178439836 62-63 0.012753677871935747 64-65 0.016459463517743494 66-67 0.0023582272291503836 68-69 6.256521220194895E-4 70-71 0.0 72-73 0.0 74-75 0.0011069229851114045 76-77 0.001636320934512511 78-79 0.008037223413634982 80-81 0.014630634237994218 82-83 0.01535254053263209 84-85 0.0271918037646932 86-87 0.010106688124930215 88-89 0.03797227109795209 90-91 0.04341063185089074 92-93 0.02762494754147592 94-95 0.028972505958133287 96-97 0.045961367425277885 98-99 0.04913775512168453 100-101 0.05630869098175406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1038916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.99570286106065 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16875083633799 33.82270777068969 2 13.278812419787428 11.949789959770337 3 4.5432661847148905 6.132823657984077 4 2.116444826002489 3.8092369005054882 5 1.1649399397353206 2.6208645689656196 6 0.700228257141239 1.8904357555947338 7 0.4921102748238411 1.5499993390593916 8 0.3481238183850314 1.2531260712728574 9 0.2803410822888766 1.1352729634576602 >10 1.6371313429972691 14.438069507254395 >50 0.16212892710217475 5.043345309474616 >100 0.0986914962996808 8.49468818796085 >500 0.004730311343878342 1.5195104071420227 >1k 0.0036552405839059914 3.2700529581943436 >5k 2.150141519944701E-4 0.7125426046479687 >10k+ 4.300283039889402E-4 2.357534038026029 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13020 1.2532293274913466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11348 1.0922923508734104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7365 0.7089119813343908 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4774 0.4595174200801605 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3739 0.35989435142013404 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2847 0.2740356294445364 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2764 0.2660465331172106 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2225 0.2141655340759022 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2174 0.20925657127236463 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1785 0.17181369812381367 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1756 0.16902232711788057 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1603 0.15429543870726797 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1538 0.14803891748707307 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1455 0.1400498211597473 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1338 0.12878808296339644 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1222 0.11762259893966402 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1204 0.11589002383253315 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1167 0.11232861944565296 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1115 0.10732340246949706 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.10530206484451102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.625417261838301E-5 0.0 14-15 0.0 0.0 0.0 9.625417261838301E-5 0.0 16-17 0.0 0.0 0.0 3.8501669047353204E-4 0.0 18-19 0.0 0.0 0.0 7.700333809470641E-4 0.0 20-21 0.0 0.0 0.0 8.66287553565447E-4 0.0 22-23 0.0 0.0 0.0 0.001251304244038979 0.0 24-25 0.0 0.0 0.0 0.0015400667618941282 0.0 26-27 0.0 0.0 0.0 0.0016363209345125111 0.0 28-29 0.0 0.0 0.0 0.0024063543154595754 0.0 30-31 0.0 0.0 0.0 0.004668327371991576 0.0 32-33 0.0 0.0 0.0 0.007122808773760342 0.0 34-35 0.0 0.0 0.0 0.009529163089219916 0.0 36-37 0.0 0.0 0.0 0.012513042440389791 0.0 38-39 0.0 0.0 0.0 0.015208159273704515 0.0 40-41 0.0 0.0 0.0 0.020405884595097196 0.0 42-43 0.0 0.0 0.0 0.02791371005933107 0.0 44-45 0.0 0.0 0.0 0.03797227109795209 0.0 46-47 0.0 0.0 0.0 0.06237270385671219 0.0 48-49 0.0 0.0 0.0 0.10977788387126583 0.0 50-51 0.0 0.0 0.0 0.14799079040076388 0.0 52-53 0.0 0.0 0.0 0.18283480088861853 0.0 54-55 0.0 0.0 0.0 0.2270154661204563 0.0 56-57 0.0 0.0 0.0 0.26489148304579 0.0 58-59 0.0 0.0 0.0 0.31580994036091464 0.0 60-61 0.0 0.0 0.0 0.40234244154484095 0.0 62-63 0.0 0.0 0.0 0.4697684894640183 0.0 64-65 0.0 0.0 0.0 0.5432104231718444 0.0 66-67 0.0 0.0 0.0 0.663480011858514 0.0 68-69 0.0 0.0 0.0 0.8055030435569381 0.0 70-71 0.0 0.0 0.0 0.9291896553715604 0.0 72-73 0.0 0.0 0.0 1.0650042929360988 0.0 74-75 0.0 0.0 0.0 1.1788729791436459 0.0 76-77 0.0 0.0 0.0 1.2732501953959705 0.0 78-79 0.0 0.0 0.0 1.387215135776136 0.0 80-81 0.0 0.0 0.0 1.5946428777687514 0.0 82-83 0.0 0.0 0.0 1.825267875362397 0.0 84-85 0.0 0.0 0.0 2.0695128383815438 0.0 86-87 0.0 0.0 0.0 2.44654043252775 0.0 88-89 0.0 0.0 0.0 2.8846412992003203 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTCC 40 5.392153E-4 47.49247 8 TACCTGG 905 0.0 29.922464 2 GTACATG 11380 0.0 25.055643 1 GTATCAA 18790 0.0 24.734814 1 TACATGG 11545 0.0 24.608114 2 GAGTACT 10980 0.0 23.877146 12-13 GGTATCA 13895 0.0 23.803852 1 GTACCTG 1160 0.0 23.761103 1 ACATGGG 11555 0.0 23.595512 3 ATGGGAG 2535 0.0 22.106945 5 AGTACTT 11170 0.0 22.04658 12-13 GTACTTT 12055 0.0 21.76656 14-15 CATGGGA 8830 0.0 21.729282 4 CGTCACT 285 8.367351E-11 21.676794 1 CATGGGG 2500 0.0 21.656565 4 AAGTTCG 110 0.0028798305 21.587484 9 ATGGGAT 3020 0.0 21.544598 5 TCAACGC 22525 0.0 20.620481 4 ATCAACG 22515 0.0 20.590424 3 CAACGCA 22790 0.0 20.401546 5 >>END_MODULE