FastQCFastQC Report
Thu 26 May 2016
SRR936487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697528
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91320.5379587258649048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78340.4614945968490652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60320.3553402359195253No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55480.32682818781192413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.22037928093085946No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34940.20582871092553406No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34210.20152834003327194No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27640.16282500200291247No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22280.13124967599945334No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20730.12211875150218436No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19430.11446055676253941No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17400.1025019911306323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16350.027.020312
GTACATG95200.026.9436451
GTACCTG17250.026.1597791
TACATGG96300.025.9961762
ACATGGG95450.025.4328883
GAGTACT73750.022.3185712-13
CATGGGA61500.021.86064
CGTACAC1856.5476725E-620.5432433
CATGGGG33700.020.4403724
GTACTTT79750.020.4333614-15
AGTACTT76100.020.19364712-13
ATGGGAT25000.019.1936555
ATGGGAG25250.019.1917725
AGAGTAC135000.018.5106610-11
GTACACG3105.5861165E-918.3873481
ACTTTTT94550.017.53949416-17
GTATCAA201750.017.3286131
GGACCGT2202.9137424E-517.2760186
GTGTAGG9350.017.2729631
TAAGGTG6900.017.2134235
Produced by FastQC (version 0.11.5)