FastQCFastQC Report
Thu 26 May 2016
SRR936487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697528
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103450.6094155737048226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91840.5410220037607627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79060.46573605855102246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49890.2938979504314509No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49570.29201285634169216No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33010.19445923719667657No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32510.19151377768142852No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27050.15934935977491976No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25990.1531049856025939No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23830.1403806004967223No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.13395949875348154No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20870.1229434801664538No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19530.11504964866558903No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17970.1058598149780151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16100.028.3230532
GTACCTG17700.027.3745611
GAGTACT89850.022.5737912-13
GTACATG91400.021.8804471
AGTACTT91850.021.61682112-13
TACATGG93350.020.9641652
GTACTTT97950.020.63430214-15
GTATAGG4250.020.1188831
ACATGGG93100.020.1009083
TATCACG1707.956557E-519.5588722
GTAAGCG1704.1836756E-1119.55714294-95
AGAGTAC152800.018.69850710-11
TACTTTT107600.018.43057414-15
ACCTGGG24650.018.1125663
ACTTTTT114800.017.77038416-17
GTACACG3551.5843398E-917.3954331
ATCACGC1952.2770981E-417.0503223
CATGGGA60450.016.9717484
AGTACAT73750.016.9390242
GAGTACA88150.016.8132841
Produced by FastQC (version 0.11.5)