##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936487_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697528 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90451055888327 31.0 31.0 34.0 30.0 34.0 2 32.0112033498122 33.0 31.0 34.0 30.0 34.0 3 32.12806681244728 33.0 31.0 34.0 30.0 34.0 4 35.86487940110561 37.0 35.0 37.0 35.0 37.0 5 35.73607622377952 37.0 35.0 37.0 33.0 37.0 6 35.79478571192935 37.0 35.0 37.0 35.0 37.0 7 35.720022880329516 37.0 35.0 37.0 33.0 37.0 8 35.69397559274427 37.0 35.0 37.0 33.0 37.0 9 37.290938352710526 39.0 37.0 39.0 34.0 39.0 10-11 37.19210228049258 39.0 37.0 39.0 33.0 39.0 12-13 37.0651650517694 39.0 37.0 39.0 33.0 39.0 14-15 38.13099194829187 40.0 37.0 41.0 33.0 41.0 16-17 38.12441444264837 40.0 37.5 41.0 33.0 41.0 18-19 38.294757435518 40.0 38.0 41.0 33.5 41.0 20-21 38.31111681221164 40.0 38.0 41.0 34.0 41.0 22-23 38.21273728621855 40.0 37.5 41.0 33.0 41.0 24-25 38.092790811108856 40.0 37.0 41.0 33.0 41.0 26-27 37.88166233487754 40.0 37.0 41.0 33.0 41.0 28-29 37.88262461650117 40.0 37.0 41.0 33.0 41.0 30-31 37.74040958381835 40.0 37.0 41.0 33.0 41.0 32-33 37.52408296063452 40.0 36.0 41.0 32.5 41.0 34-35 37.31630582823965 39.0 36.0 41.0 32.0 41.0 36-37 37.112810215796145 39.0 36.0 41.0 31.0 41.0 38-39 36.90895496274583 39.0 35.0 41.0 31.0 41.0 40-41 36.68514215965804 39.0 35.0 41.0 30.5 41.0 42-43 36.39831066114962 39.0 35.0 40.0 30.0 41.0 44-45 36.0714709860456 39.0 35.0 40.0 30.0 41.0 46-47 35.64243859306003 38.5 35.0 40.0 28.5 41.0 48-49 35.19849864037589 38.0 34.5 40.0 26.5 41.0 50-51 34.87559144827066 38.0 34.0 40.0 26.0 41.0 52-53 34.61110774019633 38.0 34.0 40.0 24.5 41.0 54-55 34.284538458275804 38.0 33.5 40.0 24.0 41.0 56-57 33.936917682653835 37.5 33.0 40.0 23.0 41.0 58-59 33.5733466546649 37.0 33.0 40.0 22.5 41.0 60-61 33.18009776569223 36.5 32.0 39.0 22.0 40.5 62-63 32.86526113265879 36.0 32.0 39.0 21.0 40.0 64-65 32.5528321182331 36.0 31.5 39.0 20.0 40.0 66-67 32.093164589921344 35.0 31.0 38.5 18.5 40.0 68-69 31.63541573393782 35.0 31.0 38.0 17.5 40.0 70-71 31.119316441319377 35.0 30.0 37.5 13.5 39.5 72-73 30.558160454496186 34.0 29.5 37.0 9.0 39.0 74-75 30.023727738216984 34.0 29.0 36.0 7.5 39.0 76-77 28.64751597617241 32.5 27.0 34.5 7.0 36.5 78-79 29.420036075988143 34.0 29.0 35.0 6.0 37.0 80-81 29.248706354180904 34.0 29.0 35.0 2.0 37.0 82-83 28.835511107916922 34.0 29.0 35.0 2.0 36.5 84-85 28.38940270793766 33.0 27.5 35.0 2.0 36.0 86-87 27.968048538816443 33.0 27.0 35.0 2.0 35.5 88-89 27.46199974315593 33.0 26.0 35.0 2.0 35.0 90-91 26.871396819374997 32.0 25.5 34.0 2.0 35.0 92-93 26.279247234802607 32.0 25.0 34.0 2.0 35.0 94-95 25.530223360085962 31.5 23.0 34.0 2.0 35.0 96-97 24.67945241551244 31.0 19.0 34.0 2.0 35.0 98-99 23.55208220424052 31.0 2.0 34.0 2.0 35.0 100-101 19.75307358700416 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19930535250725612 1101 2 0.32896298121207224 1101 3 0.34572337013929655 1101 4 0.2510157293754247 1101 5 0.1858994342206728 1101 6 0.2849722518142528 1101 7 0.23109200990104029 1101 8 0.27463837179438855 1101 9 0.3532636839560439 1101 10-11 0.3463807420394076 1101 12-13 0.34963498773159785 1101 14-15 0.4150861925316818 1101 16-17 0.3476976470685855 1101 18-19 0.377890150736782 1101 20-21 0.3706295497386378 1101 22-23 0.3607343376219987 1101 24-25 0.45337662209108487 1101 26-27 0.19798416549144093 1101 28-29 0.2774241967948896 1101 30-31 0.49779121299853557 1101 32-33 0.2922777803960628 1101 34-35 0.38635399645664137 1101 36-37 0.4753546447038559 1101 38-39 0.4423111159365263 1101 40-41 0.3369921227170849 1101 42-43 0.4965793387443469 1101 44-45 0.4203149014289451 1101 46-47 0.45517665404322827 1101 48-49 0.3483708762983966 1101 50-51 0.31657503527301145 1101 52-53 0.3314887215324447 1101 54-55 0.40282733691699235 1101 56-57 0.3905860777835102 1101 58-59 0.4797918197742206 1101 60-61 0.4147976230469652 1101 62-63 0.274896902399405 1101 64-65 0.38868487608174007 1101 66-67 0.42182872364286794 1101 68-69 0.4804151557125067 1101 70-71 0.3153840104321013 1101 72-73 0.4185632059048352 1101 74-75 0.37411591361358987 1101 76-77 0.34984315256150467 1101 78-79 0.3960093983758064 1101 80-81 0.5251023852616008 1101 82-83 0.6555240262202027 1101 84-85 0.5668390437245705 1101 86-87 0.4533523502209391 1101 88-89 0.38086391201869674 1101 90-91 0.515620538498343 1101 92-93 0.5112853331121627 1101 94-95 0.5436297902279605 1101 96-97 0.628388012002727 1101 98-99 0.16658635508802888 1101 100-101 0.12372542222893657 1102 1 0.37468924445695606 1102 2 0.5198749459102174 1102 3 0.37753002421863613 1102 4 0.22875350870153 1102 5 0.2043126999437206 1102 6 0.22941741307682406 1102 7 0.3590028291238099 1102 8 0.38071777803959606 1102 9 0.4333709772937908 1102 10-11 0.45395883579061547 1102 12-13 0.4457615534415922 1102 14-15 0.5512284135496515 1102 16-17 0.5200067476482033 1102 18-19 0.5164722414658911 1102 20-21 0.474316217064775 1102 22-23 0.4598690306159625 1102 24-25 0.3268173634530669 1102 26-27 0.47731994747550033 1102 28-29 0.45485926382455233 1102 30-31 0.6593080790123267 1102 32-33 0.5425996317365787 1102 34-35 0.6879337202522819 1102 36-37 0.711381708889185 1102 38-39 0.6078505860932424 1102 40-41 0.6911165867163263 1102 42-43 0.681509814553884 1102 44-45 0.6579702179973381 1102 46-47 0.6227975848458982 1102 48-49 0.6686686927270031 1102 50-51 0.7109312752465229 1102 52-53 0.7637241403764463 1102 54-55 0.7688868227863495 1102 56-57 0.671548320105309 1102 58-59 0.6360594266195463 1102 60-61 0.7080326877828114 1102 62-63 0.5021364931249295 1102 64-65 0.6459354914701052 1102 66-67 0.6666868079894286 1102 68-69 0.5963941787467419 1102 70-71 0.3389827385100084 1102 72-73 0.06452314724043973 1102 74-75 0.44064237065396483 1102 76-77 0.488176507399384 1102 78-79 0.5357926970868228 1102 80-81 0.6596047056018897 1102 82-83 0.7440860102007498 1102 84-85 0.46038061989295187 1102 86-87 0.5881207892921125 1102 88-89 0.48932602172914486 1102 90-91 0.5678543588379164 1102 92-93 0.6754771851838406 1102 94-95 0.8862625843706944 1102 96-97 0.79492851867845 1102 98-99 0.8130057768451806 1102 100-101 0.9229629427836947 1103 1 0.2514009621963744 1103 2 0.2909386540473875 1103 3 0.2931575966105626 1103 4 0.241950512866012 1103 5 0.22440016633789384 1103 6 0.20436966542771273 1103 7 0.1931502041182398 1103 8 0.2303025458299075 1103 9 0.24013796471111704 1103 10-11 0.2884767365971612 1103 12-13 0.3791616929336641 1103 14-15 0.38182816862752844 1103 16-17 0.40159156409853836 1103 18-19 0.3523551573367598 1103 20-21 0.4166064416713411 1103 22-23 0.27329686577201784 1103 24-25 0.250419097371946 1103 26-27 0.22410027584957248 1103 28-29 0.3278718059458825 1103 30-31 0.4871625977038576 1103 32-33 0.4336204379520865 1103 34-35 0.3505433573701495 1103 36-37 0.40799760693020914 1103 38-39 0.30394338887010264 1103 40-41 0.4911314888488576 1103 42-43 0.5871458619000904 1103 44-45 0.49431439035114266 1103 46-47 0.4460081124422146 1103 48-49 0.39385055956885395 1103 50-51 0.3246603970547284 1103 52-53 0.3898706922054558 1103 54-55 0.4541492219688692 1103 56-57 0.2955857639942252 1103 58-59 0.46775808725237056 1103 60-61 0.19556102210962223 1103 62-63 0.3203639300797789 1103 64-65 0.3245127129016012 1103 66-67 0.09261053745590431 1103 68-69 0.3298061791197995 1103 70-71 0.2473305684756646 1103 72-73 0.3969684351253129 1103 74-75 0.46279484684096417 1103 76-77 0.39832273077322355 1103 78-79 0.24808878117134725 1103 80-81 0.5101941001495334 1103 82-83 0.30015401664009644 1103 84-85 0.5579799573056121 1103 86-87 0.31461951804897126 1103 88-89 0.22542344720834606 1103 90-91 0.3687720058013291 1103 92-93 0.4686730944977704 1103 94-95 0.5473723776061412 1103 96-97 0.7932882703501285 1103 98-99 0.4724444023616847 1103 100-101 0.7450304666777967 1104 1 0.2774145670724941 1104 2 0.3649979842174673 1104 3 0.3250800749144318 1104 4 0.22736035172179925 1104 5 0.12090899198278748 1104 6 0.20531019342197254 1104 7 0.18991540559105857 1104 8 0.24470642947927956 1104 9 0.20863090728406775 1104 10-11 0.2820189591808031 1104 12-13 0.2853624641414072 1104 14-15 0.35229530593641556 1104 16-17 0.2820166991418418 1104 18-19 0.2742434248804102 1104 20-21 0.25430232459199686 1104 22-23 0.34386199575966714 1104 24-25 0.31269030857909996 1104 26-27 0.3824340977279448 1104 28-29 0.33254229650183476 1104 30-31 0.5400885727294309 1104 32-33 0.5590190749429595 1104 34-35 0.5362607589661437 1104 36-37 0.5450584443405333 1104 38-39 0.527601355652827 1104 40-41 0.4435787212569764 1104 42-43 0.5331346865111541 1104 44-45 0.4362197664268095 1104 46-47 0.284058111717286 1104 48-49 0.22706301740426227 1104 50-51 0.26099793565765594 1104 52-53 0.3352961140719799 1104 54-55 0.1970568087896254 1104 56-57 0.3466325741733627 1104 58-59 0.3603306582537229 1104 60-61 0.19497461046977094 1104 62-63 0.05132249326567262 1104 64-65 0.27510095264546663 1104 66-67 0.3480418975085584 1104 68-69 0.3650017515779638 1104 70-71 0.2148647020142498 1104 72-73 0.39676371899519225 1104 74-75 0.5275139838750071 1104 76-77 0.4338590239960638 1104 78-79 0.47397151901555645 1104 80-81 0.4178404918819645 1104 82-83 0.22333298865308038 1104 84-85 0.2922298956858995 1104 86-87 0.36499119050568396 1104 88-89 0.4134009211846532 1104 90-91 0.6194337479340213 1104 92-93 0.6401869159096627 1104 94-95 0.505261844133873 1104 96-97 0.8404758899254645 1104 98-99 0.5720358841534576 1104 100-101 0.4169824026714579 1105 1 0.15550834095659738 1105 2 0.2583269058360713 1105 3 0.20558369395442355 1105 4 0.11360352127366014 1105 5 0.13634832393211127 1105 6 0.15857882029638404 1105 7 0.08438988020709814 1105 8 0.13069061212662803 1105 9 0.18289367852055705 1105 10-11 0.1849438023007366 1105 12-13 0.21382830703918643 1105 14-15 0.21282879538866695 1105 16-17 0.27798531311110963 1105 18-19 0.35215163567866625 1105 20-21 0.22015288296069002 1105 22-23 0.2475486257301469 1105 24-25 0.20268746237489665 1105 26-27 0.406767228546677 1105 28-29 0.2702071833578614 1105 30-31 0.19760490893980887 1105 32-33 0.3644490491856942 1105 34-35 0.06701950515132182 1105 36-37 0.28549758477507226 1105 38-39 0.26893781647154924 1105 40-41 0.2443995241222794 1105 42-43 0.4384873477120905 1105 44-45 0.44151411874445756 1105 46-47 0.5524190752310645 1105 48-49 0.3879473619257894 1105 50-51 0.5886614495000515 1105 52-53 0.43339911602189574 1105 54-55 0.4827961176982498 1105 56-57 0.6977612149429078 1105 58-59 0.6184052158398643 1105 60-61 0.493708638874061 1105 62-63 0.4702301283410435 1105 64-65 0.4359609895223713 1105 66-67 0.330993988761513 1105 68-69 0.4531508467148484 1105 70-71 0.35557826839061946 1105 72-73 0.5226371000324015 1105 74-75 0.48571441086457057 1105 76-77 0.3873287413850761 1105 78-79 0.3255640674997302 1105 80-81 0.4830256395280692 1105 82-83 0.4385899985358357 1105 84-85 0.5024307681682956 1105 86-87 0.5549242015834892 1105 88-89 0.6607350355054216 1105 90-91 0.7845138865553629 1105 92-93 0.7280069002702696 1105 94-95 0.7837215290950112 1105 96-97 0.8653476192213923 1105 98-99 1.1148437087989898 1105 100-101 0.9405724561600621 1106 1 0.1316085691378568 1106 2 0.2283103185314843 1106 3 0.2258728063276223 1106 4 0.11817786981700351 1106 5 0.09507449343867336 1106 6 0.10594051327378651 1106 7 0.17872530722436863 1106 8 0.16242547261322926 1106 9 0.1877677160307769 1106 10-11 0.10294274915645474 1106 12-13 0.2058247491498335 1106 14-15 0.30339807461695045 1106 16-17 0.2342674090375354 1106 18-19 0.14919758290744767 1106 20-21 0.2029491277619897 1106 22-23 0.28008547872508416 1106 24-25 0.2967329360838207 1106 26-27 0.18284891664777803 1106 28-29 0.30296301812143867 1106 30-31 0.2742726554650261 1106 32-33 0.10132495819989629 1106 34-35 0.25152011101969407 1106 36-37 0.24678902975562522 1106 38-39 0.27199029816145526 1106 40-41 0.0032117060554881505 1106 42-43 -0.013752192381041084 1106 44-45 0.17453428369191215 1106 46-47 0.07891601033288254 1106 48-49 0.18310303933855465 1106 50-51 0.05242307091331355 1106 52-53 -0.08340321963313357 1106 54-55 0.053852597498583066 1106 56-57 0.0014329127345220627 1106 58-59 -0.049374043764721876 1106 60-61 -0.0629821007368605 1106 62-63 -0.016943917578529977 1106 64-65 -0.09338002773824172 1106 66-67 -0.21586472341121876 1106 68-69 -0.27475485228568175 1106 70-71 -0.17958029867847358 1106 72-73 -0.035480971776689074 1106 74-75 -0.10474170897038348 1106 76-77 -0.06634617340674609 1106 78-79 -0.1514812250412838 1106 80-81 0.10454655256916823 1106 82-83 0.15651227355322916 1106 84-85 0.28492952510812586 1106 86-87 0.3622741440443349 1106 88-89 0.20173499769155612 1106 90-91 0.27224498145855947 1106 92-93 0.1368420527137495 1106 94-95 0.09370221580603655 1106 96-97 0.17123693070997703 1106 98-99 0.41148710771209807 1106 100-101 0.28238338459196655 1107 1 0.06233288796892822 1107 2 0.04461816885955727 1107 3 0.014936347638972336 1107 4 0.045607802569207934 1107 5 -0.05705454878746252 1107 6 -0.015575845497941998 1107 7 0.020101882401171167 1107 8 -0.048342880171972524 1107 9 -0.13795841714718193 1107 10-11 0.006535101589122405 1107 12-13 0.041368756718405564 1107 14-15 0.06151000050672906 1107 16-17 0.09700241048194158 1107 18-19 0.04982768082777511 1107 20-21 -0.03449787671593896 1107 22-23 -0.08994580855811307 1107 24-25 -0.061316997353856095 1107 26-27 -0.2019864347724436 1107 28-29 -0.01703228144165081 1107 30-31 0.01017596900103257 1107 32-33 -0.17674423941267037 1107 34-35 -0.20116161336605387 1107 36-37 -0.14589099180980725 1107 38-39 -0.1789331146386317 1107 40-41 -0.22538702515296194 1107 42-43 -0.13000980134929563 1107 44-45 -0.10304351697148206 1107 46-47 -0.11611879955783877 1107 48-49 -0.0378587059597848 1107 50-51 -0.12970389855071573 1107 52-53 -0.0408444084084536 1107 54-55 -0.12566056998113595 1107 56-57 0.041573093472848655 1107 58-59 0.001204887941305799 1107 60-61 -0.088612402669213 1107 62-63 -0.27910425796850546 1107 64-65 -0.19859609822739088 1107 66-67 -0.08459250990257772 1107 68-69 -0.05459365014894502 1107 70-71 -0.1950304008748951 1107 72-73 -0.19569038603370004 1107 74-75 -0.039500569484371084 1107 76-77 -0.22151744285657315 1107 78-79 -0.24335300539160798 1107 80-81 -0.18220199636134637 1107 82-83 -0.04414782773465831 1107 84-85 -0.1627869547651386 1107 86-87 0.02550089605875172 1107 88-89 -0.007011868003790056 1107 90-91 -0.14326004077330978 1107 92-93 -0.14161735188669056 1107 94-95 -0.1441117382896273 1107 96-97 -0.1570118790839068 1107 98-99 0.14932799139423736 1107 100-101 0.09025533794448393 1108 1 -0.050063267363377406 1108 2 0.11954249910170489 1108 3 -0.13667284221162035 1108 4 0.0638192592925435 1108 5 0.03323557228414842 1108 6 -0.06497531741963058 1108 7 0.07186482675974304 1108 8 0.0534756090088635 1108 9 0.014694854362346632 1108 10-11 0.04942093685444604 1108 12-13 0.0726195617158325 1108 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2316 50-51 -1.8275809296012397 2316 52-53 -1.9923035163497431 2316 54-55 -1.8277657449313196 2316 56-57 -1.7596374157458925 2316 58-59 -1.7973515221400298 2316 60-61 -1.441049456396744 2316 62-63 -1.475995894581935 2316 64-65 -1.5983181187153157 2316 66-67 -1.519657861468552 2316 68-69 -1.602002243822966 2316 70-71 -1.4967716264676305 2316 72-73 -1.2650931904507488 2316 74-75 -1.6682898119772709 2316 76-77 -1.7247389843548682 2316 78-79 -1.7804260787256467 2316 80-81 -1.9422566404917063 2316 82-83 -1.8183445631565078 2316 84-85 -1.822022040393584 2316 86-87 -2.235353960771974 2316 88-89 -2.3261700049306846 2316 90-91 -2.830497915507692 2316 92-93 -2.4164792932120136 2316 94-95 -2.6517155693790535 2316 96-97 -2.423811690384749 2316 98-99 -2.081700708671356 2316 100-101 -1.4285850933324014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 12.0 5 57.0 6 134.0 7 318.0 8 729.0 9 1387.0 10 2347.0 11 3825.0 12 5588.0 13 7610.0 14 9827.0 15 12809.0 16 16345.0 17 21185.0 18 18592.0 19 12267.0 20 11471.0 21 12042.0 22 12668.0 23 14231.0 24 16778.0 25 19695.0 26 23922.0 27 29463.0 28 35932.0 29 44635.0 30 55002.0 31 67599.0 32 83322.0 33 106049.0 34 138333.0 35 186140.0 36 255668.0 37 308279.0 38 157334.0 39 5926.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9223340782327 19.300120601818985 14.90696220691313 21.870583113035188 2 15.714403544327663 21.452019606918977 39.022069566856764 23.811507281896592 3 20.253159336855916 25.34244436733436 30.024112937860732 24.380283357948993 4 12.86672659730265 17.14503174634967 37.401606345264085 32.5866353110836 5 13.152955398612967 38.28662759597417 33.68527591025523 14.875141095157637 6 30.79748905467244 36.688997177071606 17.317711401520327 15.19580236673563 7 27.951583515019845 33.53943780371495 20.720214948739958 17.788763732525254 8 26.772846592863843 33.50264153788875 19.544493772002998 20.180018097244414 9 26.45016753773723 16.93114929473917 20.751822650348036 35.86686051717557 10-11 25.115579831378334 26.735494200979304 26.84845257338907 21.30047339425329 12-13 26.89048428067166 24.22342959880485 27.528883176006524 21.357202944516967 14-15 23.023070728516146 25.45079024152307 27.17209645370685 24.354042576253935 16-17 21.54789691409154 28.736027636618992 27.581683906170284 22.13439154311918 18-19 21.281056001244185 27.185068848387452 30.401479118090275 21.132396032278095 20-21 22.604034120363824 25.549006786370704 30.397609453791414 21.449349639474054 22-23 21.907151664103125 25.884304229520577 30.42277532943628 21.78576877694002 24-25 22.184808208671228 25.938544537272566 30.59866087155277 21.277986382503435 26-27 21.940076322836884 25.804347032092583 30.972102930971307 21.283473714099223 28-29 21.26106314593927 26.828393994090234 30.68037758434618 21.23016527562432 30-31 21.619581283037185 25.9779124710828 30.526904264399985 21.87560198148003 32-33 21.589144763681638 26.154389497524694 30.586848105691626 21.669617633102035 34-35 22.416889935128435 25.993043920853392 30.313877754920743 21.27618838909743 36-37 22.289527121960013 25.65241570098127 30.611917886255014 21.446139290803707 38-39 21.808010354413014 26.264838148966046 30.53853728599029 21.388614210630653 40-41 21.916817253328738 26.33400637103081 30.250791224295337 21.49838515134512 42-43 21.773852678793656 26.523549072066505 29.563357120155803 22.13924112898404 44-45 22.079419362630308 26.14399877462893 29.481455952917475 22.295125909823295 46-47 22.565518801457177 26.793902663166673 28.957489950092135 21.683088585284015 48-49 22.585827381168038 26.784051643263012 28.693634106562538 21.936486869006412 50-51 21.946930597880545 27.110779122162835 28.955242168984864 21.987048110971756 52-53 22.5236293309149 27.481304338477408 28.071960292650537 21.923106037957147 54-55 22.0583791107642 26.884964875333207 28.39666573393617 22.659990279966422 56-57 22.584730297350298 27.00682213921005 28.08471365367547 22.323733909764183 58-59 23.156130522123842 26.713588887121574 27.998456544663004 22.131824046091584 60-61 22.399786977277675 26.98431166591015 28.923902404252445 21.691998952559732 62-63 22.264276875795456 26.995160311193967 28.767238685228186 21.973324127782394 64-65 22.000473646631335 27.524966597308463 28.39611704020341 22.078442715856795 66-67 22.27704093249277 27.139179076231752 27.981734471193032 22.602045520082452 68-69 22.19487158339973 27.0750705816339 28.285702082912984 22.444355752053387 70-71 22.5380821904896 27.116487289441142 28.065706911614914 22.279723608454344 72-73 22.41998836515733 26.805676035906007 28.475644150546024 22.298691448390638 74-75 22.01273590407952 27.887376384907327 27.79800472014356 22.301882990869597 76-77 22.245799280945526 27.19214118115566 27.85222682439601 22.709832713502802 78-79 22.338274575324583 27.260467982472157 27.975290648009267 22.42596679419399 80-81 22.579445088373777 27.311496028666067 27.770441728977264 22.338617153982895 82-83 22.350234758378548 27.154592957837746 28.135217291412616 22.35995499237109 84-85 22.102696846717613 27.417980902514934 27.92575674476775 22.5535655059997 86-87 22.234017203326854 27.359699043398887 27.65619030815828 22.75009344511598 88-89 21.975735486225474 27.466429297917315 27.332555915333113 23.225279300524093 90-91 22.22003588409279 26.954721297365193 27.631723517513013 23.19351930102901 92-93 22.404752408988056 27.23801573728849 27.800078586294998 22.557153267428454 94-95 22.038660482356185 27.033766582632385 27.89820335117994 23.029369583831485 96-97 21.61846121944524 26.974284929950997 28.263396260204555 23.143857590399204 98-99 21.79915406004053 27.338499693670766 27.418999481596686 23.443346764692023 100-101 21.36532481567676 27.08667220227942 27.73663050845261 23.81137247359121 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 715.0 1 555.5 2 626.0 3 817.5 4 620.0 5 497.5 6 803.0 7 1539.5 8 2326.0 9 2789.0 10 2612.5 11 2217.0 12 2192.0 13 2323.0 14 2557.5 15 2745.5 16 2951.5 17 3177.0 18 3311.5 19 3551.5 20 3973.5 21 4463.5 22 5027.0 23 5903.5 24 6939.5 25 8249.0 26 10175.5 27 12524.5 28 14866.5 29 17778.5 30 21210.5 31 24649.5 32 28430.5 33 31247.0 34 33792.5 35 36263.5 36 39303.0 37 42895.5 38 44809.0 39 45689.5 40 46816.5 41 49258.5 42 51601.5 43 53469.0 44 55403.5 45 57572.5 46 59264.0 47 65724.5 48 82089.5 49 81818.5 50 65241.5 51 56644.5 52 53345.0 53 52615.0 54 51556.0 55 48663.0 56 44561.5 57 40338.0 58 37369.0 59 33351.0 60 28259.0 61 23940.5 62 20071.0 63 15831.5 64 11576.0 65 8637.5 66 6377.5 67 4943.5 68 3722.0 69 2469.5 70 1820.0 71 1426.5 72 1195.5 73 1051.0 74 836.5 75 649.0 76 487.0 77 297.0 78 167.5 79 112.0 80 71.0 81 45.0 82 32.0 83 22.0 84 13.0 85 6.0 86 1.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012194202393126947 2 0.009896743971233465 3 0.005832009840191148 4 5.890919030496109E-4 5 0.0054196455080564206 6 0.0 7 4.123643321347276E-4 8 0.0018850940897587553 9 0.0 10-11 0.0 12-13 0.0 14-15 5.301827127446499E-4 16-17 0.004123643321347277 18-19 0.001767275709148833 20-21 4.712735224396888E-4 22-23 4.123643321347276E-4 24-25 3.534551418297666E-4 26-27 1.178183806099222E-4 28-29 0.0 30-31 5.301827127446499E-4 32-33 0.0036229152037551074 34-35 0.0011781838060992218 36-37 0.0017083665188438717 38-39 0.005154554151684096 40-41 2.945459515248055E-5 42-43 0.0011487292109467412 44-45 0.004889462795311771 46-47 0.0 48-49 0.00215018544613108 50-51 8.836378545744165E-4 52-53 5.007281175921694E-4 54-55 1.767275709148833E-4 56-57 0.010986563991875245 58-59 0.0022385492315885216 60-61 0.003328369252230302 62-63 8.836378545744165E-5 64-65 0.003534551418297666 66-67 7.952740691169747E-4 68-69 0.0015021843527765078 70-71 0.004771644414701849 72-73 0.0023858222073509243 74-75 0.0071869212172052535 76-77 0.001237092996404183 78-79 0.006715647694765565 80-81 0.0029749141104005354 82-83 0.0022385492315885216 84-85 0.0015905481382339495 86-87 4.4181892728720825E-4 88-89 0.0016789119236913913 90-91 0.00698073905113789 92-93 0.0018261848994537942 94-95 3.829097369822471E-4 96-97 1.178183806099222E-4 98-99 4.712735224396888E-4 100-101 0.0020323670655211577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1697528.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.732468610911894 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14730141024066 46.48516395715342 2 12.632541537140538 14.838806986122947 3 3.741620535773719 6.592638320138199 4 1.5219690102228802 3.5755598847878387 5 0.7703453941056873 2.2622143339436414 6 0.4825110522969087 1.700343914006775 7 0.3283529384738411 1.3499485056550888 8 0.21994298639014284 1.0334235635479427 9 0.16643595407957673 0.8797675003835322 >10 0.8813473480876055 9.591633596580614 >50 0.06857667899345841 2.7590801320970257 >100 0.034437173176413434 3.813218645461654 >500 0.0024093814010367736 0.9874963960107415 >1k 0.0019074269424874458 2.5130804508460605 >5k 2.007817834197311E-4 1.0076614167556468 >10k+ 1.0039089170986555E-4 0.6099623965089037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10345 0.6094155737048226 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9184 0.5410220037607627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7906 0.46573605855102246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4989 0.2938979504314509 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4957 0.29201285634169216 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3301 0.19445923719667657 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3251 0.19151377768142852 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2705 0.15934935977491976 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2599 0.1531049856025939 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2383 0.1403806004967223 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2274 0.13395949875348154 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2087 0.1229434801664538 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1953 0.11504964866558903 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1797 0.1058598149780151 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 2.9454595152480547E-5 0.0 0.0 2.9454595152480547E-5 0.0 18-19 1.1781838060992219E-4 0.0 0.0 5.8909190304961095E-5 0.0 20-21 1.767275709148833E-4 0.0 0.0 5.8909190304961095E-5 0.0 22-23 1.767275709148833E-4 0.0 0.0 1.767275709148833E-4 0.0 24-25 2.9454595152480546E-4 0.0 0.0 2.3563676121984438E-4 0.0 26-27 2.9454595152480546E-4 0.0 0.0 3.829097369822471E-4 0.0 28-29 5.596373078971304E-4 0.0 0.0 8.836378545744165E-4 0.0 30-31 5.890919030496109E-4 0.0 0.0 0.002209094636436041 0.0 32-33 5.890919030496109E-4 0.0 0.0 0.004182552511652238 0.0 34-35 5.890919030496109E-4 0.0 0.0 0.006185464982020915 0.0 36-37 5.890919030496109E-4 0.0 0.0 0.009042560711811528 0.0 38-39 7.363648788120137E-4 0.0 0.0 0.01399093269742826 0.0 40-41 7.658194739644942E-4 0.0 0.0 0.02259167448195258 0.0 42-43 8.836378545744165E-4 0.0 0.0 0.031634235193764106 0.0 44-45 9.425470448793775E-4 0.0 0.0 0.04147206997469261 0.0 46-47 9.72001640031858E-4 0.0 0.0 0.061766286034751705 0.0 48-49 0.0010014562351843385 0.0 0.0 0.07466739871153819 0.0 50-51 0.0010014562351843385 0.0 0.0 0.08983651521506567 0.0 52-53 0.0010309108303368192 0.0 0.0 0.10907036584963548 0.0 54-55 0.0010603654254892998 0.0 0.0 0.1293056727193896 0.0 56-57 0.0011192746157942608 0.0 0.0 0.18512213053334026 0.0 58-59 0.0011192746157942608 0.0 0.0 0.2366676720501812 0.0 60-61 0.0011192746157942608 0.0 0.0 0.27369209815684925 0.0 62-63 0.0011192746157942608 0.0 0.0 0.3226456353002719 0.0 64-65 0.0011192746157942608 0.0 0.0 0.376341362263244 0.0 66-67 0.0011781838060992218 0.0 0.0 0.46093495954116814 0.0 68-69 0.001237092996404183 0.0 0.0 0.5676489577786051 0.0 70-71 0.0012960021867091441 0.0 0.0 0.6257334194192967 0.0 72-73 0.0012960021867091441 0.0 0.0 0.6854084291982223 0.0 74-75 0.0012960021867091441 0.0 0.0 0.7980722556564604 0.0 76-77 0.0012960021867091441 0.0 0.0 0.9366561258488815 0.0 78-79 0.0012960021867091441 0.0 0.0 1.070379987841143 0.0 80-81 0.0014138205673190664 0.0 0.0 1.1679925161764637 0.0 82-83 0.0014138205673190664 0.0 0.0 1.308373116673186 0.0 84-85 0.001502184352776508 0.0 0.0 1.5699004670320607 0.0 86-87 0.0016494573285389107 0.0 0.0 1.8378194645390238 0.0 88-89 0.0017083665188438717 0.0 0.0 2.157284003562828 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1610 0.0 28.323053 2 GTACCTG 1770 0.0 27.374561 1 GAGTACT 8985 0.0 22.57379 12-13 GTACATG 9140 0.0 21.880447 1 AGTACTT 9185 0.0 21.616821 12-13 TACATGG 9335 0.0 20.964165 2 GTACTTT 9795 0.0 20.634302 14-15 GTATAGG 425 0.0 20.118883 1 ACATGGG 9310 0.0 20.100908 3 TATCACG 170 7.956557E-5 19.558872 2 GTAAGCG 170 4.1836756E-11 19.557142 94-95 AGAGTAC 15280 0.0 18.698507 10-11 TACTTTT 10760 0.0 18.430574 14-15 ACCTGGG 2465 0.0 18.112566 3 ACTTTTT 11480 0.0 17.770384 16-17 GTACACG 355 1.5843398E-9 17.395433 1 ATCACGC 195 2.2770981E-4 17.050322 3 CATGGGA 6045 0.0 16.971748 4 AGTACAT 7375 0.0 16.939024 2 GAGTACA 8815 0.0 16.813284 1 >>END_MODULE