FastQCFastQC Report
Thu 26 May 2016
SRR936486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741299
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91600.526044062507358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78960.4534545761526309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62950.3615117219960501No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55830.32062270753041267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38580.22155873287700734No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36590.2101304830474261No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35950.206455066016807No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26720.15344866102834723No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22180.12737617146739302No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20870.11985305223284456No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18920.108654515967677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17050.028.429722
GTACCTG19100.026.8727511
GTACATG97550.025.7234021
TACATGG99100.024.6962132
ACATGGG100700.022.9817873
GAGTACT71050.021.60032812-13
CATGGGA62750.020.8258744
AGTACTT70950.020.32489212-13
ATCACGC1456.1296276E-419.6638053
GTACTTT77650.019.60914214-15
GGTATAC2352.5162753E-618.2010481
AGAGTAC136650.018.03037310-11
CATGGGG37850.017.702624
ATGGGAG25550.017.6717455
ACCTGGG26650.017.4748383
GTATCAA204450.017.155011
TGCACCG4204.7293724E-1116.9741745
ACTTTTT93250.016.8131716-17
TACTTTT86400.016.57851414-15
ATGGGAT25150.016.440995
Produced by FastQC (version 0.11.5)