FastQCFastQC Report
Thu 26 May 2016
SRR936486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741299
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104680.6011603980706358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.5401140183276967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81450.4677542455373833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.29805334982676723No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49980.28702709873491No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31840.18285199727329998No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31710.18210542818895548No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27010.15511408437034652No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26150.1501752427354521No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23920.1373687115193887No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.11973819545063771No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20800.11945105349512058No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19310.11089422322071052No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17930.1029691052484381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG17150.026.8664971
TACCTGG17350.024.6409932
GTACATG94950.024.1622241
TACATGG99750.023.1916262
ACATGGG99100.022.095563
AGTACTT96500.020.98913812-13
GAGTACT95150.020.28872112-13
GTACCGT1200.00476943519.78816
GTACACG3403.6379788E-1119.7630081
CGCGGAA2050.019.69581682-83
CATGGGA64200.019.0130124
CATGGGG36200.018.7620474
GTACTTT103600.018.47345714-15
AGAGTAC165800.018.27422710-11
GTATAGG5100.017.8808171
TACTTTT114550.017.64035614-15
ATGGGAT25900.017.6030045
TAAGGTG7050.017.5145765
ATGGGAG28150.017.3769745
GTATCAA224000.017.3556521
Produced by FastQC (version 0.11.5)