##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1741299 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.478639797071036 31.0 31.0 34.0 30.0 34.0 2 31.677286324749513 31.0 31.0 34.0 30.0 34.0 3 31.957211828640574 31.0 31.0 34.0 30.0 34.0 4 35.76298728707706 37.0 35.0 37.0 35.0 37.0 5 35.58158822809868 37.0 35.0 37.0 33.0 37.0 6 35.69026801255844 37.0 35.0 37.0 33.0 37.0 7 35.62527572806278 37.0 35.0 37.0 33.0 37.0 8 35.60991191059089 37.0 35.0 37.0 33.0 37.0 9 37.22999611209792 39.0 37.0 39.0 33.0 39.0 10-11 37.11664165660234 39.0 37.0 39.0 33.0 39.0 12-13 36.990737374798925 39.0 37.0 39.0 33.0 39.0 14-15 38.078861240947134 40.0 37.0 41.0 33.0 41.0 16-17 38.00393671621014 40.0 37.0 41.0 33.0 41.0 18-19 38.0977000503647 40.0 37.0 41.0 33.0 41.0 20-21 38.17990362367405 40.0 37.0 41.0 33.0 41.0 22-23 38.096639635122976 40.0 37.0 41.0 33.0 41.0 24-25 37.98100699535232 40.0 37.0 41.0 33.0 41.0 26-27 37.77189069769178 40.0 37.0 41.0 32.5 41.0 28-29 37.68143839742629 39.5 37.0 41.0 32.5 41.0 30-31 37.64858476344384 39.5 37.0 41.0 33.0 41.0 32-33 37.44991095727959 39.0 36.0 41.0 32.0 41.0 34-35 37.252238989398144 39.0 36.0 41.0 31.5 41.0 36-37 37.00601160398071 39.0 35.5 41.0 31.0 41.0 38-39 36.852044077438734 39.0 35.0 41.0 31.0 41.0 40-41 36.6199211048763 39.0 35.0 41.0 30.5 41.0 42-43 36.35410259811785 39.0 35.0 40.0 30.0 41.0 44-45 36.047953567997226 39.0 35.0 40.0 30.0 41.0 46-47 35.607228856158535 38.5 35.0 40.0 28.0 41.0 48-49 35.12654805406768 38.0 34.5 40.0 26.0 41.0 50-51 34.910110785109275 38.0 34.0 40.0 26.0 41.0 52-53 34.651385258936 38.0 34.0 40.0 25.0 41.0 54-55 34.387037206131744 38.0 34.0 40.0 24.0 41.0 56-57 34.10307017921678 38.0 33.0 40.0 23.5 41.0 58-59 33.738941445438144 37.0 33.0 40.0 22.5 41.0 60-61 33.35349988715321 37.0 32.5 39.5 22.0 41.0 62-63 33.070870941750954 36.0 32.0 39.0 22.0 40.5 64-65 32.64199715270037 36.0 31.5 39.0 20.5 40.0 66-67 32.22082451089675 35.0 31.0 38.5 19.5 40.0 68-69 31.771437587685973 35.0 31.0 38.0 18.0 40.0 70-71 31.28897305976745 35.0 30.0 37.5 16.0 39.5 72-73 30.87629838413736 34.0 30.0 37.0 13.0 39.0 74-75 30.515583193925913 34.0 30.0 36.0 10.0 39.0 76-77 29.08960178579325 32.5 28.0 35.0 8.0 37.0 78-79 29.8928664749707 34.0 29.0 35.0 7.5 37.5 80-81 29.721250055274826 34.0 29.0 35.0 7.0 37.0 82-83 29.37144367509543 34.0 29.0 35.0 2.0 37.0 84-85 28.917055026161506 34.0 29.0 35.0 2.0 36.0 86-87 28.54172459755619 34.0 29.0 35.0 2.0 36.0 88-89 28.11786459419089 33.0 27.5 35.0 2.0 35.0 90-91 27.651861627440205 33.0 26.5 35.0 2.0 35.0 92-93 27.198021993925224 33.0 26.0 35.0 2.0 35.0 94-95 26.65421705290131 32.5 25.0 34.0 2.0 35.0 96-97 25.957148370268406 32.0 24.5 34.0 2.0 35.0 98-99 25.091261753438097 32.0 21.0 34.0 2.0 35.0 100-101 22.263026338383014 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49153479137347844 1101 2 0.4117921860477409 1101 3 0.30096364325661895 1101 4 0.15307248942196594 1101 5 0.29307412211192485 1101 6 0.2506893329446882 1101 7 0.25938312488735704 1101 8 0.24631279640905035 1101 9 0.31255204838970485 1101 10-11 0.27768707671403803 1101 12-13 0.253880544190757 1101 14-15 0.3816531988867595 1101 16-17 0.35164336085301784 1101 18-19 0.40529787923049554 1101 20-21 0.1669703663381199 1101 22-23 0.19876767807249962 1101 24-25 0.15169470263020202 1101 26-27 0.35224951278346595 1101 28-29 0.3868486218719198 1101 30-31 0.29236267565771357 1101 32-33 0.3033277123560296 1101 34-35 0.4534155172510097 1101 36-37 0.40861017665312005 1101 38-39 0.38320761492055766 1101 40-41 0.47914915638942546 1101 42-43 0.6921059513915608 1101 44-45 0.6720410123105296 1101 46-47 0.613688143845657 1101 48-49 0.588665359574847 1101 50-51 0.5296442362892009 1101 52-53 0.49424573967922214 1101 54-55 0.44382254605671534 1101 56-57 0.5235766765030192 1101 58-59 0.5251813457813626 1101 60-61 0.545395934248198 1101 62-63 0.49585285406669755 1101 64-65 0.3145772705921033 1101 66-67 0.10905858964834891 1101 68-69 -0.0804463020212225 1101 70-71 -0.10793066448255573 1101 72-73 0.1306500164840294 1101 74-75 0.04040139351322125 1101 76-77 0.38458586677269935 1101 78-79 0.4217709359076025 1101 80-81 0.30383434387076136 1101 82-83 0.26240345334789694 1101 84-85 0.34455410629827554 1101 86-87 0.3707528360056642 1101 88-89 0.43261103826355196 1101 90-91 0.2594445676125794 1101 92-93 0.20756360827573772 1101 94-95 -0.02878959520445079 1101 96-97 -0.12295459128054986 1101 98-99 -0.3331522248037935 1101 100-101 -0.34830586299769095 1102 1 0.46325293633684694 1102 2 0.5820526983461747 1102 3 0.25224620729392555 1102 4 0.1761968190527412 1102 5 0.12472754803953734 1102 6 0.2536322311212871 1102 7 0.16310737816421295 1102 8 0.09498260207014653 1102 9 0.22922956216067547 1102 10-11 0.3298220777139065 1102 12-13 0.3202804569291331 1102 14-15 0.499877061304268 1102 16-17 0.5866418155951436 1102 18-19 0.48135728985095483 1102 20-21 0.48838145947171085 1102 22-23 0.32417771679484275 1102 24-25 0.1618248678818972 1102 26-27 0.09659695947207325 1102 28-29 0.1996171786010592 1102 30-31 0.3089469285709754 1102 32-33 0.47606827264835516 1102 34-35 0.44226184590008444 1102 36-37 0.4817652296463777 1102 38-39 0.40481032003081197 1102 40-41 0.5083221707330594 1102 42-43 0.2867224547456786 1102 44-45 0.18059337837302536 1102 46-47 0.5226050089719152 1102 48-49 0.7232733185618798 1102 50-51 0.5496860491493791 1102 52-53 0.43559465890768223 1102 54-55 0.48564158726964735 1102 56-57 0.45586383995494373 1102 58-59 0.49374280159607054 1102 60-61 0.7107567065135427 1102 62-63 0.7148749785235822 1102 64-65 0.6988581802945006 1102 66-67 0.746765972345095 1102 68-69 0.610306511257086 1102 70-71 0.45665693345952363 1102 72-73 0.5908539905237014 1102 74-75 0.23759047853876325 1102 76-77 0.5800646240217766 1102 78-79 0.821681856335907 1102 80-81 0.9045364363317212 1102 82-83 0.8749545832040937 1102 84-85 0.9498634305660971 1102 86-87 0.9961496036897728 1102 88-89 0.5731631498129914 1102 90-91 0.4631073741644762 1102 92-93 0.8002130892508852 1102 94-95 0.9922337290997305 1102 96-97 0.8721187630198095 1102 98-99 0.656376744236514 1102 100-101 0.24764083469532494 1103 1 0.5282934480639234 1103 2 0.5082445960007576 1103 3 0.387734480215034 1103 4 0.24561259730434415 1103 5 0.12630537319945034 1103 6 0.1868689048952703 1103 7 0.13323104554161347 1103 8 0.22505509673965918 1103 9 0.18256005256579755 1103 10-11 0.20619307984610202 1103 12-13 0.3546813098075958 1103 14-15 0.3427128821997911 1103 16-17 0.23943072817509403 1103 18-19 0.3593956693818754 1103 20-21 0.20235160872545066 1103 22-23 0.2858962669014531 1103 24-25 0.280933609886155 1103 26-27 0.17643917695608025 1103 28-29 0.3431054515221632 1103 30-31 0.3425460756925176 1103 32-33 0.3460682726483526 1103 34-35 0.37263284803228913 1103 36-37 0.3671426283670627 1103 38-39 0.5506695950841234 1103 40-41 0.5398573519697294 1103 42-43 0.502982582677447 1103 44-45 0.5603375148335843 1103 46-47 0.5450485057736145 1103 48-49 0.6595931479861861 1103 50-51 0.34494404488498986 1103 52-53 0.6091724840675994 1103 54-55 0.6407673868432084 1103 56-57 0.5645333495498264 1103 58-59 0.6716361917879681 1103 60-61 0.5462663013962725 1103 62-63 0.5225039763913912 1103 64-65 0.5437792890365003 1103 66-67 0.44229262479712617 1103 68-69 0.48178199100122043 1103 70-71 0.4938424345256216 1103 72-73 0.3015746728264723 1103 74-75 0.5326352546581674 1103 76-77 0.3769686751944832 1103 78-79 0.5299334554830324 1103 80-81 0.603150508826392 1103 82-83 0.6107584211571861 1103 84-85 0.6957568207579925 1103 86-87 0.5480216719200541 1103 88-89 0.48374310716906876 1103 90-91 0.40873637203227986 1103 92-93 0.5018889954342534 1103 94-95 0.36735099988864306 1103 96-97 0.06522324063175233 1103 98-99 -0.009329012693125804 1103 100-101 0.11811418224330339 1104 1 0.3497855814811679 1104 2 0.4129840547798622 1104 3 0.13942485151774164 1104 4 0.051426945951291714 1104 5 0.01564772688101357 1104 6 0.09880219627349618 1104 7 0.07089750243273585 1104 8 0.09424389472581396 1104 9 0.08974570391884384 1104 10-11 0.09543914655472463 1104 12-13 0.07290094479815679 1104 14-15 0.17868204519538722 1104 16-17 0.09964595787931785 1104 18-19 0.14206558756941234 1104 20-21 0.07095953824086365 1104 22-23 0.11016561869502794 1104 24-25 0.21067275400195484 1104 26-27 0.2866635318962878 1104 28-29 0.09759978758258114 1104 30-31 0.08249384158300899 1104 32-33 0.07781937396993754 1104 34-35 0.16639213059993807 1104 36-37 0.2695689971524615 1104 38-39 0.26257299344787555 1104 40-41 0.27043161251094716 1104 42-43 0.30471574819034686 1104 44-45 0.3896452555510166 1104 46-47 0.3302366744331522 1104 48-49 0.43699151173697715 1104 50-51 0.22590785860430884 1104 52-53 0.2982879655024604 1104 54-55 0.22369438495523042 1104 56-57 0.3797639977562426 1104 58-59 0.24130296334240597 1104 60-61 0.10788587345417255 1104 62-63 -0.06038966740974416 1104 64-65 0.0604167969030911 1104 66-67 0.07050093190851214 1104 68-69 0.21741792555125272 1104 70-71 0.1552694326376418 1104 72-73 0.18560676848411717 1104 74-75 0.16126741324847416 1104 76-77 0.24913639073885108 1104 78-79 0.40799670280838995 1104 80-81 0.2953846183292228 1104 82-83 0.36271594161030407 1104 84-85 0.41883328394238717 1104 86-87 0.43176585064881223 1104 88-89 0.5748636232168991 1104 90-91 0.4364991158963427 1104 92-93 0.5205840866866147 1104 94-95 0.38320059082634117 1104 96-97 0.2051329322617086 1104 98-99 0.48546708548182593 1104 100-101 0.31807988394248454 1105 1 0.3210327675062885 1105 2 0.4472018737702044 1105 3 0.2638106307172059 1105 4 0.14198435566140688 1105 5 0.030301775537928677 1105 6 0.04367717951677008 1105 7 0.13393978486105595 1105 8 0.07011940493932656 1105 9 0.16170141068301547 1105 10-11 0.10906641218008417 1105 12-13 0.1110467722570121 1105 14-15 0.21963838062581686 1105 16-17 0.2244026963957566 1105 18-19 0.16300832115822317 1105 20-21 0.19630334010005157 1105 22-23 0.1927312241792194 1105 24-25 -0.06984558363804894 1105 26-27 0.13438791027942187 1105 28-29 0.11276607211878087 1105 30-31 0.16813864052537042 1105 32-33 0.23733794735979075 1105 34-35 0.08879894007245781 1105 36-37 0.20597518720381913 1105 38-39 0.015933873303282553 1105 40-41 -0.033747804678441184 1105 42-43 0.05719574412250239 1105 44-45 -0.1783778201986408 1105 46-47 0.01556956708376589 1105 48-49 0.05101932097374373 1105 50-51 -0.1041121685762576 1105 52-53 0.06642596930219469 1105 54-55 0.08593962488847495 1105 56-57 0.08582640906114136 1105 58-59 0.08379808655637078 1105 60-61 0.12217156297624854 1105 62-63 0.1516222495138635 1105 64-65 0.15343571236134323 1105 66-67 0.10553231899589122 1105 68-69 0.06860285743803729 1105 70-71 0.0027586389927165555 1105 72-73 -0.1006022121982646 1105 74-75 0.052643649201712606 1105 76-77 -0.0010828912873037666 1105 78-79 0.03578265350431664 1105 80-81 -0.20749407219594573 1105 82-83 0.13448379966116164 1105 84-85 0.4007686630604965 1105 86-87 0.20276578824444158 1105 88-89 0.24630374275593425 1105 90-91 0.15822820234257762 1105 92-93 0.27760717861518813 1105 94-95 0.4849630520547379 1105 96-97 0.4009084452487528 1105 98-99 0.5530955113662337 1105 100-101 0.5510570693666708 1106 1 0.2495468850035536 1106 2 0.3307144107048856 1106 3 0.01781636903750794 1106 4 -0.022469889246764296 1106 5 -0.05374244509104642 1106 6 -0.03080353981482631 1106 7 0.065128236217042 1106 8 0.016185999309890065 1106 9 0.10506513325915989 1106 10-11 -0.04777643599370407 1106 12-13 0.06363648016026247 1106 14-15 0.1483300370115117 1106 16-17 0.08664322070349328 1106 18-19 -0.03498568148483372 1106 20-21 -0.07738479294819456 1106 22-23 0.08354151495883144 1106 24-25 -0.047876312409123045 1106 26-27 0.022270318617763962 1106 28-29 -9.005257641518938E-4 1106 30-31 -0.1375262494718683 1106 32-33 -0.0365049491089664 1106 34-35 -0.31001240002509434 1106 36-37 -0.2656421893257104 1106 38-39 -0.1117473206361339 1106 40-41 -0.09336739399559946 1106 42-43 -0.25450217524245744 1106 44-45 -0.12121359096438056 1106 46-47 -0.25676081879303325 1106 48-49 -0.4418999781345576 1106 50-51 -0.3843533251090321 1106 52-53 -0.27834604551997444 1106 54-55 -0.3525249024574464 1106 56-57 -0.45867466001383406 1106 58-59 -0.45664922363342697 1106 60-61 -0.27648803213629947 1106 62-63 -0.6222963822457928 1106 64-65 -0.5827768974548277 1106 66-67 -0.6075214815985746 1106 68-69 -0.546712629441096 1106 70-71 -0.5592866748587184 1106 72-73 -0.2813293917282209 1106 74-75 -0.2584881763041764 1106 76-77 -0.3328959392705464 1106 78-79 -0.2163247632856482 1106 80-81 -0.29801805064760956 1106 82-83 -0.15440983945249798 1106 84-85 -0.05802859466340493 1106 86-87 -0.2421911194726576 1106 88-89 -0.21921804046990445 1106 90-91 -0.3364489238433954 1106 92-93 -0.0436531444342485 1106 94-95 -0.09802855779216912 1106 96-97 -0.07011567150214049 1106 98-99 -0.17743750043969087 1106 100-101 -0.10950476575430912 1107 1 0.551434459724021 1107 2 0.5496659532694466 1107 3 0.36855018359561953 1107 4 0.2335495153481304 1107 5 0.10542289689713158 1107 6 0.21813876480635486 1107 7 0.20237188943054463 1107 8 0.2799937763806284 1107 9 0.40799013393480976 1107 10-11 0.41136194151878414 1107 12-13 0.43132873871457633 1107 14-15 0.4370816432097726 1107 16-17 0.45325490399927304 1107 18-19 0.4122351962672042 1107 20-21 0.29174444489690643 1107 22-23 0.3374226489163803 1107 24-25 0.37137921008748265 1107 26-27 0.3690594269351095 1107 28-29 0.31707206506129637 1107 30-31 0.4440290049769686 1107 32-33 0.43305006947748126 1107 34-35 0.403883917573431 1107 36-37 0.5369775415454825 1107 38-39 0.4712742423452596 1107 40-41 0.5124460609706531 1107 42-43 0.47906730711331846 1107 44-45 0.4819279854316889 1107 46-47 0.43191588267152525 1107 48-49 0.5390037679006241 1107 50-51 0.6299533561843802 1107 52-53 0.41653663137067554 1107 54-55 0.41666655637596506 1107 56-57 0.40305746665409714 1107 58-59 0.3072818590977633 1107 60-61 0.4222929770107342 1107 62-63 0.15026993562300106 1107 64-65 0.14811348918750156 1107 66-67 0.18444697426444634 1107 68-69 0.11123270822293208 1107 70-71 0.13497824527975055 1107 72-73 0.26578304460736035 1107 74-75 0.5438319663434292 1107 76-77 0.40609945281381243 1107 78-79 0.5895568092285224 1107 80-81 0.7042905138595259 1107 82-83 0.4949771947500068 1107 84-85 0.4830971445563321 1107 86-87 0.855172749849757 1107 88-89 0.9314439711905429 1107 90-91 0.724386065849913 1107 92-93 0.9106270207677021 1107 94-95 1.0712843947380684 1107 96-97 0.9766489599506016 1107 98-99 1.1184861034884044 1107 100-101 1.0845106254947083 1108 1 0.31255194422449506 1108 2 0.24244302981813703 1108 3 0.054143056205301576 1108 4 0.1267977261722777 1108 5 -0.04813543878260873 1108 6 -0.06764154899357067 1108 7 -0.008394135025561411 1108 8 -0.017936464120985818 1108 9 0.10118971880163485 1108 10-11 -0.013218917416885745 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-1.42269022938277 2316 82-83 -1.3985778741264738 2316 84-85 -1.7439120104108383 2316 86-87 -1.6171823608892453 2316 88-89 -1.5138467766313433 2316 90-91 -1.5609201556505674 2316 92-93 -1.9420460695008117 2316 94-95 -2.1095235729069834 2316 96-97 -2.1661810655883436 2316 98-99 -2.20504705780591 2316 100-101 -2.859962714544121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 10.0 4 16.0 5 86.0 6 146.0 7 362.0 8 785.0 9 1542.0 10 2604.0 11 4138.0 12 5895.0 13 8115.0 14 9854.0 15 12955.0 16 16598.0 17 20778.0 18 18072.0 19 11651.0 20 10732.0 21 11232.0 22 11916.0 23 13049.0 24 15821.0 25 18390.0 26 22963.0 27 28498.0 28 35085.0 29 43577.0 30 54108.0 31 66605.0 32 82557.0 33 104036.0 34 134933.0 35 180430.0 36 251220.0 37 325018.0 38 204734.0 39 12783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72918843679232 19.40126521539587 14.994363217659867 21.87518313015194 2 15.567984579739313 21.5690666629908 39.089166187080934 23.77378257018895 3 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21.445110805209215 22-23 21.949195492402872 25.84755776274097 30.46220242838253 21.741044316473634 24-25 22.192656172202476 25.96618960902177 30.585011534492352 21.2561426842834 26-27 21.948671652599582 25.735815618110387 31.023592157349196 21.291920571940835 28-29 21.261885523393744 26.84886398028139 30.684879506621204 21.204370989703662 30-31 21.602700513438677 25.947618975149865 30.592514723925852 21.857165787485602 32-33 21.55677927579334 26.17742233289753 30.6249693104786 21.640829080830528 34-35 22.397338294407678 25.96354598129719 30.3175606353797 21.321555088915428 36-37 22.28251755641369 25.65889674278849 30.629825572981158 21.428760127816666 38-39 21.77862874013702 26.26700851922691 30.5526945009078 21.401668239728274 40-41 21.957339376567496 26.294931574271306 30.242770775388216 21.50495827377298 42-43 21.75676296594107 26.499597955315608 29.56053644247889 22.183102636264433 44-45 22.053777304300635 26.158517850630723 29.50248527642419 22.285219568644454 46-47 22.599145683969688 26.69964439870532 29.025840820180143 21.675369097144852 48-49 22.610446887683924 26.781069316438565 28.689943490754 21.91854030512351 50-51 21.990369780556307 27.072149691363784 29.01892534239711 21.918555185682795 52-53 22.52843415929676 27.45397799696139 28.07108877223642 21.946499071505425 54-55 21.976720544297702 26.874986359245217 28.44539620218413 22.70289689427295 56-57 22.551817670944978 27.008994527130458 28.13636143049823 22.30282637142633 58-59 23.204263783254813 26.705701382984437 28.0208904934644 22.069144340296354 60-61 22.47765461148056 26.960774460181476 28.908526195061185 21.653044733276783 62-63 22.30191987461762 26.92795078890913 28.81859325587292 21.951536080600334 64-65 22.016519289257005 27.46159523720631 28.454992690961202 22.066892782575483 66-67 22.274503047401634 27.073331466844703 28.0609049598346 22.591260525919065 68-69 22.199091249486013 27.03650507783875 28.32790249998277 22.43650117269246 70-71 22.52025096552813 27.148038075548094 28.04447890195403 22.28723205696975 72-73 22.470580870947494 26.761371826435322 28.47193388384189 22.296113418775292 74-75 22.012819165462105 27.884958298373803 27.835914452371473 22.266308083792616 76-77 22.2763679967748 27.19999632455764 27.864820675741562 22.658815002925998 78-79 22.35944988958162 27.25499036531398 27.989696198678427 22.395863546425975 80-81 22.610012211134304 27.34935101784342 27.741072259358518 22.299564511663757 82-83 22.32915135170373 27.181971195473153 28.13680929744489 22.352068155378227 84-85 22.137220881153766 27.396190676386684 27.95702722742281 22.50956121503674 86-87 22.26835167934102 27.434927455948475 27.61992390506196 22.676796959648545 88-89 22.081011022152012 27.490927368685597 27.307851936954723 23.12020967220766 90-91 22.257973627697954 26.970907521169618 27.692554252031805 23.078564599100627 92-93 22.459427158979377 27.19438495656284 27.810005564964502 22.536182319493285 94-95 22.104075802599883 26.993132141097227 27.91088553913094 22.991906517171945 96-97 21.677153711425973 26.959597476676606 28.33874126171339 23.024507550184033 98-99 21.850321343035844 27.293223366434443 27.499956924526025 23.356498366003684 100-101 21.40678912809055 26.987710302583007 27.9007864315078 23.704714137818637 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 793.0 1 602.0 2 689.5 3 906.5 4 665.5 5 524.0 6 819.0 7 1650.0 8 2611.5 9 3024.5 10 2705.5 11 2217.0 12 2185.5 13 2410.0 14 2603.5 15 2788.0 16 3037.0 17 3204.5 18 3424.5 19 3751.0 20 4057.0 21 4541.0 22 5233.5 23 6154.0 24 7136.5 25 8498.0 26 10476.5 27 12921.5 28 15406.5 29 18209.0 30 21626.0 31 25229.0 32 29091.5 33 32069.5 34 34765.5 35 37355.0 36 40466.0 37 43857.0 38 45942.0 39 46904.5 40 47969.5 41 50518.0 42 52710.0 43 54769.0 44 57074.5 45 59127.5 46 60393.0 47 67339.5 48 84365.5 49 83561.5 50 66638.0 51 58452.0 52 54951.5 53 53754.5 54 52630.0 55 49668.0 56 45679.0 57 41258.0 58 38158.5 59 34573.5 60 29294.5 61 24434.5 62 20438.5 63 16085.5 64 11794.5 65 8935.5 66 6667.0 67 5121.5 68 3778.0 69 2464.0 70 1825.5 71 1477.0 72 1255.5 73 1119.0 74 900.0 75 677.5 76 461.0 77 283.5 78 180.5 79 119.5 80 83.0 81 56.5 82 39.0 83 23.0 84 16.0 85 10.5 86 5.5 87 2.0 88 0.0 89 1.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.022110504858729 2 0.012232247305029177 3 0.004536842897170446 4 0.014069955820338723 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003445703466205402 10-11 2.2971356441369345E-4 12-13 2.5842775996540515E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.475684532064854E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.871419555171168E-5 32-33 0.003991273181687923 34-35 5.45569715482522E-4 36-37 0.0031585615106882853 38-39 1.435709777585584E-4 40-41 0.003905130595032789 42-43 0.011428249829581249 44-45 0.002526849208550628 46-47 0.0013208529953787375 48-49 0.009647969705375126 50-51 5.45569715482522E-4 52-53 0.002268421448585223 54-55 0.011083679482960709 56-57 0.0042209867461016175 58-59 0.001636709146447566 60-61 0.006431979803583417 62-63 0.0037615596172742303 64-65 0.017458230895440702 66-67 0.0035318460528605366 68-69 4.0199873772396355E-4 70-71 0.0013782813864821606 72-73 0.0 74-75 0.0 76-77 6.891406932410803E-4 78-79 0.011141107874064133 80-81 0.015218523642407191 82-83 0.012548103456098005 84-85 0.008614258665513505 86-87 0.010595538158581611 88-89 0.027938912271815466 90-91 0.0240912100678861 92-93 0.002756562772964321 94-95 0.008327116709996389 96-97 0.010480681376374765 98-99 0.009705398096478548 100-101 0.011858962762856924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1741299.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.83968591862783 #Duplication Level Percentage of deduplicated Percentage of total 1 79.10976064422421 46.54793469403977 2 12.68771624563094 14.930824778351926 3 3.724141161569186 6.573818887900942 4 1.5302784430242113 3.601644118223656 5 0.7945872419826812 2.337663187660484 6 0.46679534031820935 1.6479654727561455 7 0.31887758011346573 1.3133859662257885 8 0.2141493698395823 1.0080385328826473 9 0.16053501962325184 0.8501247120215603 >10 0.8835147086022026 9.51156618242525 >50 0.07140656605882077 2.855166447214116 >100 0.033618145052557544 3.7240632391008117 >500 0.0025554868719441627 1.065965413792958 >1k 0.0016708952624250293 2.11134251989764 >5k 2.948638698397111E-4 1.3151070697510776 >10k+ 9.828795661323702E-5 0.6053887777552454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10468 0.6011603980706358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9405 0.5401140183276967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8145 0.4677542455373833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.29805334982676723 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4998 0.28702709873491 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3184 0.18285199727329998 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3171 0.18210542818895548 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2701 0.15511408437034652 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2615 0.1501752427354521 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2392 0.1373687115193887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.11973819545063771 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2080 0.11945105349512058 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1931 0.11089422322071052 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1793 0.1029691052484381 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.742839110342336E-5 0.0 0.0 0.0 0.0 8 5.742839110342336E-5 0.0 0.0 0.0 0.0 9 5.742839110342336E-5 0.0 0.0 0.0 0.0 10-11 5.742839110342336E-5 0.0 0.0 0.0 0.0 12-13 5.742839110342336E-5 0.0 0.0 0.0 0.0 14-15 5.742839110342336E-5 0.0 0.0 0.0 0.0 16-17 5.742839110342336E-5 0.0 0.0 0.0 0.0 18-19 5.742839110342336E-5 0.0 0.0 0.0 0.0 20-21 5.742839110342336E-5 0.0 0.0 5.742839110342336E-5 0.0 22-23 5.742839110342336E-5 0.0 0.0 3.158561510688285E-4 0.0 24-25 1.722851733102701E-4 0.0 0.0 4.881413243790986E-4 0.0 26-27 1.722851733102701E-4 0.0 0.0 8.614258665513505E-4 0.0 28-29 2.0099936886198178E-4 0.0 0.0 0.0016941375375509893 0.0 30-31 2.2971356441369345E-4 0.0 0.0 0.0028427053596194567 0.0 32-33 2.2971356441369345E-4 0.0 0.0 0.004737842266032428 0.0 34-35 4.0199873772396355E-4 0.0 0.0 0.006431979803583417 0.0 36-37 4.0199873772396355E-4 0.0 0.0 0.009332113554306297 0.0 38-39 4.594271288273869E-4 0.0 0.0 0.013610528691511338 0.0 40-41 4.594271288273869E-4 0.0 0.0 0.022483215116990245 0.0 42-43 5.45569715482522E-4 0.0 0.0 0.02983404917822844 0.0 44-45 6.31712302137657E-4 0.0 0.0 0.039108734341431306 0.0 46-47 8.039974754479271E-4 0.0 0.0 0.05794524662335417 0.0 48-49 8.614258665513505E-4 0.0 0.0 0.06914378288852173 0.0 50-51 8.614258665513505E-4 0.0 0.0 0.08396030779320496 0.0 52-53 8.614258665513505E-4 0.0 0.0 0.10001154310661178 0.0 54-55 8.614258665513505E-4 2.871419555171168E-5 0.0 0.11683806169991484 0.0 56-57 8.614258665513505E-4 5.742839110342336E-5 0.0 0.1678919013908582 0.0 58-59 8.614258665513505E-4 5.742839110342336E-5 0.0 0.21415047042466573 0.0 60-61 8.614258665513505E-4 5.742839110342336E-5 0.0 0.2514789246418909 0.0 62-63 8.614258665513505E-4 5.742839110342336E-5 0.0 0.29874249052000834 0.0 64-65 8.614258665513505E-4 5.742839110342336E-5 0.0 0.34643676933140144 0.0 66-67 8.614258665513505E-4 5.742839110342336E-5 0.0 0.4277553711338489 0.0 68-69 8.614258665513505E-4 5.742839110342336E-5 0.0 0.5252113508363583 0.0 70-71 8.614258665513505E-4 5.742839110342336E-5 0.0 0.5763800473095086 0.0 72-73 8.614258665513505E-4 5.742839110342336E-5 0.0 0.6310518756399677 0.0 74-75 8.614258665513505E-4 5.742839110342336E-5 0.0 0.7367488294658184 0.0 76-77 8.614258665513505E-4 5.742839110342336E-5 0.0 0.8615407233335572 0.0 78-79 8.614258665513505E-4 5.742839110342336E-5 0.0 0.9862464746146411 0.0 80-81 0.001091139430965044 1.1485678220684673E-4 0.0 1.0758060505404299 0.0 82-83 0.0011485678220684672 1.1485678220684673E-4 0.0 1.205163501500891 0.0 84-85 0.001177282017620179 1.1485678220684673E-4 0.0 1.45325415106768 0.0 86-87 0.0012059962131718907 1.1485678220684673E-4 0.0 1.7160177545613935 0.0 88-89 0.0012059962131718907 1.1485678220684673E-4 0.0 2.0331086160389455 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1715 0.0 26.866497 1 TACCTGG 1735 0.0 24.640993 2 GTACATG 9495 0.0 24.162224 1 TACATGG 9975 0.0 23.191626 2 ACATGGG 9910 0.0 22.09556 3 AGTACTT 9650 0.0 20.989138 12-13 GAGTACT 9515 0.0 20.288721 12-13 GTACCGT 120 0.004769435 19.7881 6 GTACACG 340 3.6379788E-11 19.763008 1 CGCGGAA 205 0.0 19.695816 82-83 CATGGGA 6420 0.0 19.013012 4 CATGGGG 3620 0.0 18.762047 4 GTACTTT 10360 0.0 18.473457 14-15 AGAGTAC 16580 0.0 18.274227 10-11 GTATAGG 510 0.0 17.880817 1 TACTTTT 11455 0.0 17.640356 14-15 ATGGGAT 2590 0.0 17.603004 5 TAAGGTG 705 0.0 17.514576 5 ATGGGAG 2815 0.0 17.376974 5 GTATCAA 22400 0.0 17.355652 1 >>END_MODULE