FastQCFastQC Report
Thu 26 May 2016
SRR936485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722245
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89600.5202511837746662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78300.45463914832094154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62320.3618532787147009No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56070.3255634360964903No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37740.2191325856658025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.21530037828531948No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34880.20252635368370933No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25590.1485851316160012No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22300.1294821584617752No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20730.12036614999608068No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19630.11397913769527564No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17360.10079866685634158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17450.028.870792
GTACATG96950.027.9415191
TACATGG99550.027.0701122
GTACCTG18500.026.9737011
ACATGGG100700.025.7698213
TATCACG1000.001648344623.7639272
GAGTACT70300.022.64906912-13
CATGGGA64650.022.6447894
GTACACG2901.05501385E-1021.3043541
GTACTTT75950.020.61999114-15
AGTACTT72000.020.03489512-13
CATGGGG37750.019.6423654
GTATAGG4650.019.4188561
ATAACGC1759.8957986E-519.0111433
ATGGGAG26500.018.6540685
ATGGGAT25300.018.4116045
TAGTACC4650.018.3994854
AGAGTAC134000.018.30044210-11
ACCTGGG27250.018.1390723
ACTTTTT90450.017.83910816-17