FastQCFastQC Report
Thu 26 May 2016
SRR936485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722245
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103990.6038049174188341No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93910.5452766592441841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82500.47902592256037907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.2949638408008152No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48400.28102854123542237No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32990.19155230527596248No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32560.1890555641038296No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26100.15154638277364718No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25490.14800449413410985No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24890.14452066924276163No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21310.12373384739105063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.12268869992364617No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19480.11310818147243859No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18060.10486312922958116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17900.026.0039182
GTACCTG19700.024.8428081
GTACCGT1351.3429952E-524.6301966
GTACATG91750.023.8739641
TACATGG93200.022.8821142
GAGTACT92250.022.26918812-13
TATCACG1302.9436097E-421.9216692
ACATGGG94000.021.2226563
GTACTTT98350.020.71803914-15
CGATTCG1150.003722899420.6532489
AGTACTT92850.020.61616112-13
ATGGGAG23600.018.9182785
AGAGTAC153400.018.85799210-11
CATGGGA58400.018.5438694
GAGTCGA1300.00756235218.270189
ACTTTTT114100.017.96173716-17
CATGGGG36800.017.8118744
TACTTTT107150.017.70884914-15
GTGTAGC8250.017.2781411
TACCGTG1650.001463988417.2731237
Produced by FastQC (version 0.11.5)