##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936485_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1722245 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83005437670018 31.0 31.0 34.0 30.0 34.0 2 31.962003083185028 31.0 31.0 34.0 30.0 34.0 3 32.06682963225325 33.0 31.0 34.0 30.0 34.0 4 35.82472122142901 37.0 35.0 37.0 35.0 37.0 5 35.60332995595865 37.0 35.0 37.0 33.0 37.0 6 35.71064453663677 37.0 35.0 37.0 33.0 37.0 7 35.65715679244242 37.0 35.0 37.0 33.0 37.0 8 35.636830996751335 37.0 35.0 37.0 33.0 37.0 9 37.25572900487445 39.0 37.0 39.0 34.0 39.0 10-11 37.12387610357412 39.0 37.0 39.0 33.0 39.0 12-13 37.02717035032762 39.0 37.0 39.0 33.0 39.0 14-15 38.08382111720458 40.0 37.0 41.0 33.0 41.0 16-17 38.02438590328322 40.0 37.0 41.0 33.0 41.0 18-19 38.2065074945783 40.0 37.5 41.0 33.0 41.0 20-21 38.24912512447416 40.0 38.0 41.0 33.0 41.0 22-23 38.154017575896574 40.0 37.0 41.0 33.0 41.0 24-25 38.02397684417723 40.0 37.0 41.0 33.0 41.0 26-27 37.789158917575605 40.0 37.0 41.0 33.0 41.0 28-29 37.70494180560838 40.0 37.0 41.0 32.5 41.0 30-31 37.64589707039359 39.5 37.0 41.0 33.0 41.0 32-33 37.470503615919924 39.0 36.0 41.0 32.0 41.0 34-35 37.2678892956577 39.0 36.0 41.0 31.5 41.0 36-37 36.99204497617934 39.0 35.5 41.0 31.0 41.0 38-39 36.821170042589756 39.0 35.0 41.0 31.0 41.0 40-41 36.60786908947333 39.0 35.0 41.0 30.5 41.0 42-43 36.33266347122506 39.0 35.0 40.0 30.0 41.0 44-45 36.0428455301075 39.0 35.0 40.0 30.0 41.0 46-47 35.63616065077849 38.5 35.0 40.0 28.5 41.0 48-49 35.2333387526165 38.0 34.5 40.0 26.5 41.0 50-51 34.89591434435867 38.0 34.0 40.0 26.0 41.0 52-53 34.62583110997564 38.0 34.0 40.0 24.5 41.0 54-55 34.35677212011067 38.0 34.0 40.0 24.0 41.0 56-57 34.03544240221339 38.0 33.0 40.0 23.5 41.0 58-59 33.70749922339737 37.0 33.0 40.0 23.0 41.0 60-61 33.32120371956371 37.0 32.5 39.5 22.0 41.0 62-63 32.92770047234859 36.0 32.0 39.0 21.0 40.0 64-65 32.51356775603935 36.0 31.0 39.0 20.0 40.0 66-67 32.04251805056772 35.0 31.0 38.5 18.5 40.0 68-69 31.760567457011053 35.0 31.0 38.0 18.0 40.0 70-71 31.320026767387915 35.0 30.5 37.5 15.5 39.5 72-73 30.861973760992193 34.5 30.0 37.0 12.5 39.0 74-75 30.348808677046527 34.0 29.0 36.0 8.0 39.0 76-77 28.933865971450054 32.5 28.0 35.0 7.5 37.0 78-79 29.71481902981283 34.0 29.0 35.0 7.0 37.0 80-81 29.49017038806906 34.0 29.0 35.0 4.0 37.0 82-83 29.14743024366452 34.0 29.0 35.0 2.0 36.5 84-85 28.724487805161285 34.0 29.0 35.0 2.0 36.0 86-87 28.34576613664142 33.0 28.0 35.0 2.0 36.0 88-89 27.92372746037875 33.0 27.0 35.0 2.0 35.0 90-91 27.42048576131735 33.0 26.0 35.0 2.0 35.0 92-93 26.925889754361314 32.5 25.5 34.5 2.0 35.0 94-95 26.31738167333916 32.0 25.0 34.0 2.0 35.0 96-97 25.51768912088582 32.0 22.5 34.0 2.0 35.0 98-99 24.544699795905924 31.5 10.5 34.0 2.0 35.0 100-101 21.28771167865199 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30386673578039947 1101 2 0.34243147910260774 1101 3 0.3480730476385929 1101 4 0.26051451954374016 1101 5 0.24212400848347926 1101 6 0.23618598090367016 1101 7 0.2670408807029787 1101 8 0.24227493567526892 1101 9 0.30812182800342924 1101 10-11 0.3884432360964496 1101 12-13 0.3208699085605744 1101 14-15 0.4707170985342586 1101 16-17 0.5178180226852973 1101 18-19 0.47804721069744005 1101 20-21 0.3019021157482271 1101 22-23 0.4113501079350428 1101 24-25 0.3484974163135064 1101 26-27 0.3984564814734881 1101 28-29 0.3484363005300395 1101 30-31 0.40585826965499905 1101 32-33 0.49555797741695073 1101 34-35 0.33334907855070384 1101 36-37 0.26783834937895534 1101 38-39 0.3237892675420184 1101 40-41 0.32987907624637813 1101 42-43 0.42812250051520095 1101 44-45 0.5088949905028102 1101 46-47 0.5384320901892963 1101 48-49 0.35689692968353626 1101 50-51 0.301007700242387 1101 52-53 0.29844569136290033 1101 54-55 0.3444948726025885 1101 56-57 0.3798969945430386 1101 58-59 0.4163500362407362 1101 60-61 0.29498705257702085 1101 62-63 0.24064577541756194 1101 64-65 0.24343390490337669 1101 66-67 0.23992491285113005 1101 68-69 0.13339434426281827 1101 70-71 0.19084557593667384 1101 72-73 0.29392953616671136 1101 74-75 0.248766785206179 1101 76-77 0.17651146969585696 1101 78-79 -0.02692436043103541 1101 80-81 0.18603417384415977 1101 82-83 0.4791594970062505 1101 84-85 0.49548436823760866 1101 86-87 0.37300886835737757 1101 88-89 0.035989695062539795 1101 90-91 -0.028464416498724887 1101 92-93 0.05849502852777988 1101 94-95 0.25069930015167685 1101 96-97 0.36649726370797353 1101 98-99 0.7372549562156081 1101 100-101 0.7646066793709885 1102 1 0.35276762055732647 1102 2 0.33638519412787105 1102 3 0.3545248467812385 1102 4 0.20316588114347667 1102 5 0.17286425054106758 1102 6 0.2728595825499198 1102 7 0.17299492838731823 1102 8 0.2814799079662791 1102 9 0.2914579449509631 1102 10-11 0.27700759909898665 1102 12-13 0.4068060930021673 1102 14-15 0.473635769574976 1102 16-17 0.45171130922642533 1102 18-19 0.3721542306975323 1102 20-21 0.2223146336269366 1102 22-23 0.25669954774127035 1102 24-25 0.4313910416995128 1102 26-27 0.1602529917147848 1102 28-29 0.09170184426611883 1102 30-31 0.2883813306958771 1102 32-33 0.3717539864702317 1102 34-35 0.38058460599061306 1102 36-37 0.5410126249347655 1102 38-39 0.433074476653303 1102 40-41 0.5195597029101009 1102 42-43 0.47055165409715727 1102 44-45 0.411943769733341 1102 46-47 0.6401312632840543 1102 48-49 0.7053796537490129 1102 50-51 0.5468984327315596 1102 52-53 0.39705436537967387 1102 54-55 0.513491527454363 1102 56-57 0.485095961100491 1102 58-59 0.2747434578387029 1102 60-61 0.34448916365628435 1102 62-63 0.401983195508933 1102 64-65 0.5209518214128224 1102 66-67 0.49509762408449376 1102 68-69 0.28502949423089063 1102 70-71 0.43463313324992114 1102 72-73 0.5648289803373743 1102 74-75 0.350950169709904 1102 76-77 0.0963036883600914 1102 78-79 0.2276273642757083 1102 80-81 0.6002222210735049 1102 82-83 0.5405112131903209 1102 84-85 0.4605807672030693 1102 86-87 0.5794064729370767 1102 88-89 0.37290083178748645 1102 90-91 0.5600436900044166 1102 92-93 0.5361041169937906 1102 94-95 0.7534244928224538 1102 96-97 1.0302316849189772 1102 98-99 1.0597825397704277 1102 100-101 1.6321994304272174 1103 1 0.20992824287410272 1103 2 0.2830351014027741 1103 3 0.24914347495997902 1103 4 0.207570642137199 1103 5 0.14093403797484427 1103 6 0.20443624082413692 1103 7 0.26344143735754955 1103 8 0.22863871914635325 1103 9 0.26581951926241487 1103 10-11 0.19038839186668355 1103 12-13 0.3601224501175082 1103 14-15 0.31750207452448365 1103 16-17 0.3828262551856696 1103 18-19 0.20181395382319778 1103 20-21 0.2692284949448833 1103 22-23 0.22165469022161233 1103 24-25 0.33589885892266125 1103 26-27 0.30607245454694976 1103 28-29 0.40638238270940974 1103 30-31 0.30991727665768565 1103 32-33 0.2495781480542334 1103 34-35 0.4029228089858776 1103 36-37 0.3555079045002927 1103 38-39 0.3394052284791016 1103 40-41 0.33468760487404126 1103 42-43 0.314757228316779 1103 44-45 0.292070753374432 1103 46-47 0.16796856198642018 1103 48-49 0.3194825416192444 1103 50-51 0.3002436149803742 1103 52-53 0.21556443759556743 1103 54-55 0.09149061678422044 1103 56-57 0.15939746179305558 1103 58-59 0.1278079776706491 1103 60-61 0.1602445862250832 1103 62-63 0.07853681918533795 1103 64-65 -0.09290267288464804 1103 66-67 0.11051486151431078 1103 68-69 0.34298682538065606 1103 70-71 0.20436750836949003 1103 72-73 0.04917699663250019 1103 74-75 0.01721507013564505 1103 76-77 -0.2101763546990476 1103 78-79 -0.1000292671630767 1103 80-81 0.04797443461417217 1103 82-83 0.10813516764150677 1103 84-85 -0.046017446148834296 1103 86-87 -0.26516515496871307 1103 88-89 -0.17807756466778812 1103 90-91 1.4959048372986672E-4 1103 92-93 0.08213919437524453 1103 94-95 0.14181032361126356 1103 96-97 0.3993863049756605 1103 98-99 0.7807526968291398 1103 100-101 1.0480797831652247 1104 1 0.12003677866281137 1104 2 0.20263048261009686 1104 3 0.1962569120117692 1104 4 0.14671633336857326 1104 5 0.055828877963016055 1104 6 0.1361807693284618 1104 7 0.2172015053840184 1104 8 0.1663629345975579 1104 9 0.22016715494127226 1104 10-11 0.2789445994641895 1104 12-13 0.14542714591438965 1104 14-15 0.13803619541743473 1104 16-17 0.21793415231389446 1104 18-19 0.21232767407530417 1104 20-21 0.18164305129481306 1104 22-23 0.1607775721497049 1104 24-25 0.16638285409074172 1104 26-27 0.13486582415619353 1104 28-29 0.06593893335882939 1104 30-31 0.021125038389875783 1104 32-33 0.10401282403439183 1104 34-35 0.17577316900791828 1104 36-37 0.16447314677159142 1104 38-39 0.16950648353820696 1104 40-41 0.057826000777858155 1104 42-43 0.10614105962281428 1104 44-45 0.10860914051947645 1104 46-47 0.24232415355025694 1104 48-49 0.21029531920846267 1104 50-51 0.13047807637061482 1104 52-53 0.13127950012955125 1104 54-55 0.17494118993639063 1104 56-57 0.1667858513102658 1104 58-59 -0.07370765491344144 1104 60-61 0.03395449203178913 1104 62-63 0.12612386861035674 1104 64-65 0.26623208812859644 1104 66-67 -0.025204142467281088 1104 68-69 0.09787303432847594 1104 70-71 0.0562822125237048 1104 72-73 0.15212404985299344 1104 74-75 0.2017952512232668 1104 76-77 0.08585464405647869 1104 78-79 0.1672004243456584 1104 80-81 0.35182765583521913 1104 82-83 0.2546379540259949 1104 84-85 0.1473703193058391 1104 86-87 0.05236178738031683 1104 88-89 -0.06476824496894551 1104 90-91 0.10913198535108748 1104 92-93 0.16145854651804115 1104 94-95 0.26094742029716755 1104 96-97 0.595822305720155 1104 98-99 0.6500417034748622 1104 100-101 0.8836867478491222 1105 1 -0.02515082329477636 1105 2 0.1346587588471806 1105 3 0.027143263289637787 1105 4 -0.0011901375395240166 1105 5 0.0052579154834120345 1105 6 0.007306381073981072 1105 7 -3.0801065731367316E-4 1105 8 0.04836402214513669 1105 9 -0.18766318763621115 1105 10-11 -0.05058231733842433 1105 12-13 -0.20449128801708838 1105 14-15 -0.055819160754388975 1105 16-17 0.07981560962765144 1105 18-19 -0.0016473123303484272 1105 20-21 -0.018465703463213856 1105 22-23 -0.009151737257575121 1105 24-25 0.06333772086615852 1105 26-27 0.011972947592845173 1105 28-29 0.14832066255948462 1105 30-31 0.02194069907503149 1105 32-33 -0.02649288559040741 1105 34-35 -0.023248038169889185 1105 36-37 -0.14874055632791539 1105 38-39 -0.04229340770702805 1105 40-41 -0.08872103565499145 1105 42-43 -0.10321184956696072 1105 44-45 0.08087667722421088 1105 46-47 0.12541278867804095 1105 48-49 0.07163300273212059 1105 50-51 0.12177769572895869 1105 52-53 0.060860794311167865 1105 54-55 0.22578403931104418 1105 56-57 0.14014093559520546 1105 58-59 0.04755390027958839 1105 60-61 0.023079016229722527 1105 62-63 0.17098520629388503 1105 64-65 0.09331202287574314 1105 66-67 0.1825174453521896 1105 68-69 0.17427325183798814 1105 70-71 0.18515660077818907 1105 72-73 0.14043291336577468 1105 74-75 0.01201819847720742 1105 76-77 -0.11697297965205777 1105 78-79 -0.12589364852003015 1105 80-81 0.26128931978301395 1105 82-83 0.1722562248253432 1105 84-85 0.15552692615738906 1105 86-87 0.149425408913757 1105 88-89 0.29792887302997073 1105 90-91 0.42832720014173375 1105 92-93 0.4417739353162986 1105 94-95 0.4395771103461037 1105 96-97 0.4193677108316294 1105 98-99 0.5880514914030819 1105 100-101 0.8711799506767441 1106 1 0.11054294118735797 1106 2 0.14383203740905515 1106 3 0.19049214520073576 1106 4 0.1582002829249518 1106 5 0.08043486253325227 1106 6 0.09970734086854094 1106 7 0.17244207014412183 1106 8 0.11005808483528767 1106 9 0.09208240034088533 1106 10-11 0.17026248185788972 1106 12-13 0.13696733150329976 1106 14-15 0.34402088328864266 1106 16-17 0.40781134295909993 1106 18-19 0.2135947640561966 1106 20-21 0.18849330705082679 1106 22-23 0.1982542911909988 1106 24-25 0.0812128505234142 1106 26-27 0.2104533218880249 1106 28-29 0.13501040946793097 1106 30-31 0.22705399606462606 1106 32-33 0.2271402379680012 1106 34-35 0.23556769956703505 1106 36-37 0.1676735893193424 1106 38-39 0.19202751176180044 1106 40-41 0.2162498920042708 1106 42-43 0.2168415220216957 1106 44-45 0.19650727689582936 1106 46-47 0.14755091074982118 1106 48-49 0.1778690205551925 1106 50-51 0.19667275679837104 1106 52-53 0.1101921642946806 1106 54-55 0.034240824587456586 1106 56-57 0.07164278939622903 1106 58-59 0.06358861004584782 1106 60-61 0.016270668405063304 1106 62-63 -0.07332303492287195 1106 64-65 0.20478212075796876 1106 66-67 0.08911143043179237 1106 68-69 0.16202393111409208 1106 70-71 0.2815469015477383 1106 72-73 0.26976255409569205 1106 74-75 0.2079118976123766 1106 76-77 -0.22904228743008304 1106 78-79 -0.058310271587743046 1106 80-81 0.11598099357403058 1106 82-83 0.0506694052667811 1106 84-85 -0.07398119824652483 1106 86-87 0.01986814194611597 1106 88-89 -0.006798694536644945 1106 90-91 0.18894306733741573 1106 92-93 0.26263385077697166 1106 94-95 0.5246635921414473 1106 96-97 0.48244120616779185 1106 98-99 0.8145544495148549 1106 100-101 1.0820838944630644 1107 1 0.06499523314132105 1107 2 0.1446619607202635 1107 3 0.12486918811355707 1107 4 0.04990883301671545 1107 5 0.008005736016848175 1107 6 0.08191174376707977 1107 7 0.05546764381328728 1107 8 0.053540335851877785 1107 9 0.0762522760348503 1107 10-11 0.13638858173435153 1107 12-13 0.031286533522482785 1107 14-15 0.26092548886409617 1107 16-17 0.2550880626551404 1107 18-19 0.07257465687605702 1107 20-21 0.16865597316022019 1107 22-23 0.04449282272566535 1107 24-25 0.13160154030230586 1107 26-27 0.03888029077487687 1107 28-29 0.06453478158717729 1107 30-31 0.28861229611027284 1107 32-33 0.17841609894572485 1107 34-35 0.16457048298898513 1107 36-37 0.20233407769971024 1107 38-39 0.17204734325116533 1107 40-41 0.1563339049899568 1107 42-43 -0.04226489775300024 1107 44-45 0.20240839941628508 1107 46-47 0.14629504946989158 1107 48-49 0.1874454973753572 1107 50-51 0.3154464431831556 1107 52-53 0.15792957553266262 1107 54-55 0.19581685779350977 1107 56-57 0.05654166334053912 1107 58-59 0.18928264802617178 1107 60-61 0.2753646863714394 1107 62-63 0.4742480210241027 1107 64-65 0.26823433373949257 1107 66-67 0.34788129078742713 1107 68-69 0.38615297492326306 1107 70-71 0.2543758224507151 1107 72-73 0.2770997204906145 1107 74-75 0.3119144600825656 1107 76-77 0.16781686195411893 1107 78-79 0.3740418553794065 1107 80-81 0.6528994663668861 1107 82-83 0.5284389500177795 1107 84-85 0.5603814186547673 1107 86-87 0.5050405684212151 1107 88-89 0.5123414296467956 1107 90-91 0.41983836670993924 1107 92-93 0.3470274436891856 1107 94-95 0.6108393391650857 1107 96-97 0.8089369141442653 1107 98-99 1.2662080667232019 1107 100-101 1.453666020235648 1108 1 0.015567652045426428 1108 2 -0.015227139665061173 1108 3 0.012464499362550896 1108 4 -0.061595954326435276 1108 5 -0.14002666705075484 1108 6 -0.019994646635730362 1108 7 0.015345109443892113 1108 8 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5 66.0 6 171.0 7 327.0 8 816.0 9 1548.0 10 2573.0 11 4042.0 12 5686.0 13 7914.0 14 9889.0 15 13065.0 16 16601.0 17 20912.0 18 18353.0 19 12170.0 20 11015.0 21 11401.0 22 12182.0 23 13557.0 24 16147.0 25 19115.0 26 23508.0 27 28721.0 28 35556.0 29 43340.0 30 53950.0 31 66402.0 32 81536.0 33 103921.0 34 134801.0 35 180535.0 36 251033.0 37 319933.0 38 190940.0 39 10481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98953747532053 19.31553294574206 14.828880332114874 21.866049246822527 2 15.724346333498247 21.56925253116486 38.94951141956598 23.756889715770914 3 20.267312049481415 25.343720930772704 30.005713681829217 24.383253337916667 4 12.886525806903016 17.183098918671284 37.33159218293925 32.59878309148646 5 13.223913563530202 38.299803278993124 33.62709872514568 14.849184432330992 6 30.794314381270905 36.66498281308064 17.34531772575251 15.195385079895948 7 27.990558834544448 33.582910677632974 20.617275706998715 17.809254780823867 8 26.854367409979417 33.472821811066375 19.520103121216785 20.15270765773743 9 26.4606540957192 16.946740408483212 20.73050849917984 35.86209699661775 10-11 25.11643287189858 26.697893495494522 26.8236601582709 21.362013474335996 12-13 26.886405858473072 24.18539173341904 27.523024740981644 21.405177667126246 14-15 23.049489623649155 25.410294159914926 27.16884908601422 24.371367130421707 16-17 21.55957323079755 28.639866960516386 27.631531960221707 22.16902784846436 18-19 21.318947071990337 27.14816997581645 30.3741337614567 21.158749190736508 20-21 22.6393747695595 25.550168530029122 30.36313068117486 21.44732601923652 22-23 21.95770575187839 25.79885264368083 30.43321991383215 21.810221690608632 24-25 22.227638334656717 25.867198148908898 30.64023190896673 21.264931607467656 26-27 21.989649641076266 25.72599856285339 30.991210179062662 21.293141617007684 28-29 21.279563336527016 26.835060825131375 30.650262752954156 21.235113085387454 30-31 21.66532779358264 25.912695797486908 30.547993446828354 21.8739829621021 32-33 21.604708524605776 26.12043167088405 30.625442012079628 21.649417792430548 34-35 22.415820506894512 25.94962876590941 30.273579723290368 21.36097100390571 36-37 22.357464857271772 25.601100781685076 30.59163821703099 21.44979614401216 38-39 21.869086048198326 26.200298125594845 30.560399467392298 21.370216358814538 40-41 21.970768970817165 26.34555231617961 30.200074206935156 21.483604506068072 42-43 21.782012073859924 26.44366354610555 29.571608487353334 22.202715892681187 44-45 22.088251509456803 26.11040191891248 29.509811578100546 22.291534993530174 46-47 22.587746738483844 26.727114952095913 28.996741749932575 21.688396559487668 48-49 22.621281710340664 26.751130801344768 28.698722005771586 21.92886548254298 50-51 21.95151974739683 27.078137783230666 28.9874099058951 21.982932563477405 52-53 22.550500842237163 27.42775370325961 28.059417059236473 21.962328395266752 54-55 22.013225552368734 26.876811095597976 28.449366840543455 22.660596511489832 56-57 22.588617838971672 26.96284575800025 28.1017296065381 22.34680679648998 58-59 23.204882958737617 26.67807432727657 27.986374677780525 22.13066803620529 60-61 22.430749854617446 26.95707563754206 28.925394289390688 21.686780218449808 62-63 22.294769646524134 26.936388628638834 28.778252534522608 21.99058919031442 64-65 22.007081460017623 27.524668247099115 28.392183392103547 22.076066900779715 66-67 22.29693007159489 27.12313972325934 27.98834623357469 22.59158397157108 68-69 22.174747167997044 26.994595837511635 28.350484934597848 22.480172059893473 70-71 22.56683721934871 27.135266547597205 28.04846467747892 22.249431555575168 72-73 22.45819878140444 26.772318539613067 28.492473559693153 22.27700911928934 74-75 22.10843272050946 27.87220180508287 27.798136849739596 22.221228624668072 76-77 22.309004138318674 27.142132984786294 27.825707511974528 22.723155364920505 78-79 22.378416011297283 27.23030756295495 27.960426992702292 22.430849433045484 80-81 22.670163041916148 27.267488094197738 27.775424473097665 22.28692439078845 82-83 22.349742403105388 27.139926865418147 28.089689301418126 22.420641430058343 84-85 22.18648546796728 27.32305516150929 27.956097019965032 22.5343623505584 86-87 22.275897388849437 27.43717160291745 27.59362927805336 22.693301730179755 88-89 22.102997562857883 27.42650103158994 27.340501330062907 23.13000007548927 90-91 22.23642701284674 26.942842251815808 27.676423064024636 23.144307671312824 92-93 22.470512000167226 27.14298239603862 27.7998067617142 22.586698842079958 94-95 22.141911928227064 26.92109378130064 27.92907248771781 23.007921802754485 96-97 21.73224981470229 26.928337325738134 28.28561947499998 23.053793384559597 98-99 21.926894287567407 27.26956533857162 27.44164265171517 23.3618977221458 100-101 21.432783301324346 26.986930751710414 27.80278979022136 23.77749615674388 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 807.0 1 600.0 2 629.5 3 806.0 4 620.0 5 557.0 6 842.0 7 1622.5 8 2514.0 9 2940.0 10 2706.0 11 2208.0 12 2135.5 13 2393.5 14 2639.5 15 2754.0 16 2931.0 17 3205.0 18 3406.0 19 3681.0 20 4062.5 21 4517.0 22 5122.5 23 5917.0 24 6883.0 25 8348.0 26 10334.0 27 12627.0 28 15247.0 29 18153.0 30 21375.0 31 24787.5 32 28579.5 33 31440.0 34 34048.5 35 36785.0 36 39803.0 37 43278.5 38 45311.5 39 46150.0 40 47116.5 41 49493.5 42 52037.5 43 54117.0 44 56233.5 45 58357.0 46 59805.0 47 66469.0 48 83096.0 49 82598.0 50 66009.5 51 57950.0 52 54458.0 53 53394.0 54 52471.0 55 49492.5 56 45416.5 57 41212.0 58 38120.5 59 34225.5 60 29023.5 61 24555.5 62 20510.5 63 16065.5 64 11893.5 65 8972.5 66 6681.0 67 5121.0 68 3862.0 69 2612.0 70 1891.0 71 1459.5 72 1227.5 73 1082.0 74 865.5 75 656.0 76 487.5 77 294.5 78 174.5 79 129.5 80 87.5 81 57.5 82 43.5 83 30.0 84 13.5 85 6.0 86 2.5 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03901883878309997 2 0.006561203545372464 3 0.003658016135915622 4 6.967649782696423E-4 5 5.225737337022317E-4 6 2.903187409456843E-4 7 0.0 8 0.0 9 0.0011612749637827371 10-11 4.6450998551309484E-4 12-13 0.0012483705860664423 14-15 0.0020612630607143583 16-17 0.0028741555353622742 18-19 0.0 20-21 0.0 22-23 0.0014515937047284214 24-25 0.0010161155933098949 26-27 0.004790259225603791 28-29 0.005516056077968001 30-31 0.00148062557882299 32-33 4.0644623732395793E-4 34-35 0.008767625976559666 36-37 0.011380494645070823 38-39 0.008825689724748802 40-41 0.001974167438430653 42-43 6.967649782696423E-4 44-45 0.0011322430896881688 46-47 8.709562228370528E-5 48-49 8.709562228370528E-4 50-51 0.0010451474674044634 52-53 0.002670932416700295 54-55 0.0028160917871731376 56-57 0.001887071816146948 58-59 6.09669355985937E-4 60-61 0.0037451117581993273 62-63 0.004441876736468969 64-65 0.008274084116952003 66-67 0.003193506150402527 68-69 0.0031354424022133906 70-71 5.8063748189136856E-5 72-73 9.580518451207581E-4 74-75 0.006270884804426781 76-77 0.002787059913078569 78-79 0.003541888639537348 80-81 0.0046741317292255165 82-83 0.004499940484658106 84-85 0.004877354847887496 86-87 0.0031354424022133906 88-89 0.008477307235613981 90-91 0.012338546490191582 92-93 0.0011032112155936003 94-95 0.002090294934808927 96-97 9.580518451207581E-4 98-99 0.0031354424022133906 100-101 0.004209621743712422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1722245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.49032951449685 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9732695123773 46.19172556616116 2 12.721090411530225 14.881215399082185 3 3.7326530702025122 6.5497232411832975 4 1.568823689105063 3.6704405810361482 5 0.8189431467478775 2.395012725346115 6 0.4767939551202561 1.67327013273024 7 0.3136126573552008 1.2840315368035882 8 0.22073497648477317 1.0328689207975277 9 0.17015311914059347 0.8957080805807479 >10 0.893484998286517 9.615061167653987 >50 0.07057502056997003 2.7895769772406824 >100 0.03479571696071357 3.750633002593589 >500 0.002982191834727969 1.2178078095974734 >1k 0.0016899087063458492 2.127224469894159 >5k 2.9821918347279686E-4 1.3210700043859724 >10k+ 9.940639449093229E-5 0.604630384913064 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10399 0.6038049174188341 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9391 0.5452766592441841 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8250 0.47902592256037907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5080 0.2949638408008152 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4840 0.28102854123542237 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3299 0.19155230527596248 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3256 0.1890555641038296 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2610 0.15154638277364718 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2549 0.14800449413410985 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2489 0.14452066924276163 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2131 0.12373384739105063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2113 0.12268869992364617 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1948 0.11310818147243859 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1806 0.10486312922958116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.03223118661979E-4 0.0 22-23 5.8063748189136856E-5 0.0 0.0 4.354781114185264E-4 0.0 24-25 5.8063748189136856E-5 0.0 0.0 5.516056077968001E-4 0.0 26-27 5.8063748189136856E-5 0.0 0.0 6.387012300805054E-4 0.0 28-29 2.6128686685111585E-4 0.0 0.0 0.0012483705860664423 0.0 30-31 2.903187409456843E-4 0.0 0.0 0.0025838367944165897 0.0 32-33 2.903187409456843E-4 0.0 0.0 0.004935418596076633 0.0 34-35 4.06446237323958E-4 0.0 0.0 0.006706362915845306 0.0 36-37 4.06446237323958E-4 0.0 0.0 0.010364379051760929 0.0 38-39 5.225737337022317E-4 0.0 0.0 0.015909467003823497 0.0 40-41 5.225737337022317E-4 0.0 0.0 0.02493837984723428 0.0 42-43 5.225737337022317E-4 0.0 0.0 0.03335762333465912 0.0 44-45 5.806374818913686E-4 0.0 0.0 0.043692970512325485 0.0 46-47 5.806374818913686E-4 0.0 0.0 0.06294110303702435 0.0 48-49 5.806374818913686E-4 0.0 0.0 0.07620866949824212 0.0 50-51 5.806374818913686E-4 0.0 0.0 0.09197297713159278 0.0 52-53 5.806374818913686E-4 0.0 0.0 0.10808566725407825 0.0 54-55 6.387012300805054E-4 0.0 0.0 0.12739186352696627 0.0 56-57 7.548287264587791E-4 0.0 0.0 0.18144921309105266 0.0 58-59 7.548287264587791E-4 0.0 0.0 0.23022276156992763 0.0 60-61 7.548287264587791E-4 0.0 0.0 0.26956095096806787 0.0 62-63 7.548287264587791E-4 0.0 0.0 0.3185957863137939 0.0 64-65 7.548287264587791E-4 0.0 0.0 0.3697789803425181 0.0 66-67 8.709562228370528E-4 0.0 0.0 0.45333271398668595 0.0 68-69 8.709562228370528E-4 0.0 0.0 0.555176528310432 0.0 70-71 8.709562228370528E-4 0.0 0.0 0.6099306428527881 0.0 72-73 8.709562228370528E-4 0.0 0.0 0.6677621360491683 0.0 74-75 8.709562228370528E-4 0.0 0.0 0.7736123489979648 0.0 76-77 8.709562228370528E-4 0.0 0.0 0.9025138699778487 0.0 78-79 8.709562228370528E-4 0.0 0.0 1.0324024746769478 0.0 80-81 9.290199710261897E-4 0.0 0.0 1.127656053581227 0.0 82-83 9.870837192153265E-4 0.0 0.0 1.2541188971371668 0.0 84-85 0.0010161155933098949 0.0 0.0 1.5010930500596604 0.0 86-87 0.001074179341499032 0.0 0.0 1.7616831519325067 0.0 88-89 0.0011032112155936003 0.0 0.0 2.078043483941019 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1790 0.0 26.003918 2 GTACCTG 1970 0.0 24.842808 1 GTACCGT 135 1.3429952E-5 24.630196 6 GTACATG 9175 0.0 23.873964 1 TACATGG 9320 0.0 22.882114 2 GAGTACT 9225 0.0 22.269188 12-13 TATCACG 130 2.9436097E-4 21.921669 2 ACATGGG 9400 0.0 21.222656 3 GTACTTT 9835 0.0 20.718039 14-15 CGATTCG 115 0.0037228994 20.653248 9 AGTACTT 9285 0.0 20.616161 12-13 ATGGGAG 2360 0.0 18.918278 5 AGAGTAC 15340 0.0 18.857992 10-11 CATGGGA 5840 0.0 18.543869 4 GAGTCGA 130 0.007562352 18.27018 9 ACTTTTT 11410 0.0 17.961737 16-17 CATGGGG 3680 0.0 17.811874 4 TACTTTT 10715 0.0 17.708849 14-15 GTGTAGC 825 0.0 17.278141 1 TACCGTG 165 0.0014639884 17.273123 7 >>END_MODULE