FastQCFastQC Report
Thu 26 May 2016
SRR936484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786759
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91810.5138353857459232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77710.4349215534943437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.35253775131397125No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57120.31968497150427116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.20713481784616727No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35930.20109035409923778No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35570.1990755328502613No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26350.14747372197369651No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22700.12704567319935145No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21040.11775510855129316No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20130.11266208817193588No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17900.10018138987966481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18600.032.7088932
TATCACG1551.3478711E-930.6645892
GTACCTG21000.029.8760721
GTACATG99900.025.549221
TACATGG100350.025.0557372
GTGTAGC9500.024.5155331
ACATGGG101300.023.6966673
GAGTACT71700.022.43799412-13
ACCTGGG27000.022.1825833
CATGGGA64150.020.7475454
AGTACTT74000.020.32761812-13
GTACTTT79600.020.02777914-15
GACCGTA1200.0047455419.8053237
CATGGGG39500.019.254324
ATGGGAT25950.018.8671025
AGAGTAC138950.018.33706510-11
GTGTAAG7950.017.9358921
CATGGGT13300.017.5125664
ACTTTTT94750.017.17481616-17
ATGGGAG27550.017.0812235
Produced by FastQC (version 0.11.5)