##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936484_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1786759 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2528410378792 31.0 31.0 34.0 28.0 34.0 2 31.282663750399465 31.0 31.0 34.0 28.0 34.0 3 31.22465368860602 31.0 31.0 34.0 27.0 34.0 4 34.89281486758986 37.0 35.0 37.0 32.0 37.0 5 34.94281545524606 37.0 35.0 37.0 32.0 37.0 6 35.01308738335724 37.0 35.0 37.0 32.0 37.0 7 34.87589708516929 37.0 35.0 37.0 32.0 37.0 8 34.868204385706186 37.0 35.0 37.0 32.0 37.0 9 36.32742300444548 39.0 37.0 39.0 32.0 39.0 10-11 36.19662472666991 39.0 35.0 39.0 32.0 39.0 12-13 36.09631433226305 39.0 35.0 39.0 30.5 39.0 14-15 37.039863238411 39.0 36.0 41.0 31.0 41.0 16-17 36.97252931145163 39.0 36.0 41.0 31.0 41.0 18-19 36.86892188593985 39.0 36.0 41.0 30.0 41.0 20-21 36.863297176619795 39.0 36.0 41.0 30.0 41.0 22-23 36.8578037105172 39.0 36.0 41.0 30.5 41.0 24-25 36.95307313409363 39.0 36.0 41.0 30.5 41.0 26-27 36.88014472013293 39.0 36.0 41.0 30.0 41.0 28-29 36.77001515033645 39.0 36.0 41.0 30.0 41.0 30-31 36.56771842201438 39.0 36.0 41.0 30.0 41.0 32-33 36.378814098599754 39.0 35.0 41.0 30.0 41.0 34-35 36.16172018722167 39.0 35.0 40.0 30.0 41.0 36-37 35.945249191413055 38.0 35.0 40.0 29.0 41.0 38-39 35.74140608778241 38.0 35.0 40.0 27.5 41.0 40-41 35.51306387710933 38.0 35.0 40.0 27.0 41.0 42-43 35.231515274303916 38.0 34.0 40.0 26.0 41.0 44-45 35.03790606343665 38.0 34.0 40.0 25.5 41.0 46-47 34.83712268974159 38.0 34.0 40.0 24.0 41.0 48-49 34.57057443113481 38.0 34.0 40.0 24.0 41.0 50-51 33.57069923811773 37.5 32.5 39.5 22.5 40.5 52-53 33.6737607030383 37.5 33.0 39.5 23.0 40.5 54-55 34.118153035747966 38.0 33.0 40.0 23.0 41.0 56-57 33.965606441607406 38.0 33.0 40.0 22.0 41.0 58-59 33.73292844754105 38.0 33.0 40.0 21.5 41.0 60-61 33.414930608996514 37.0 33.0 40.0 20.0 41.0 62-63 33.094368910412655 37.0 32.0 40.0 19.5 41.0 64-65 32.74699078051377 36.0 32.0 39.0 18.0 41.0 66-67 32.36975411905019 36.0 31.0 39.0 17.0 41.0 68-69 31.93350670123951 35.0 31.0 39.0 13.0 40.0 70-71 31.52728655627312 35.0 31.0 38.0 10.0 40.0 72-73 31.108592988757856 35.0 30.5 37.0 8.0 39.5 74-75 30.716310929453833 35.0 30.0 37.0 7.0 39.0 76-77 30.25744966164995 34.5 30.0 36.0 4.0 39.0 78-79 29.784012841127428 34.0 29.0 36.0 2.0 38.5 80-81 29.31899265653622 34.0 29.0 35.0 2.0 37.0 82-83 28.916453198220914 34.0 29.0 35.0 2.0 37.0 84-85 28.541874981460847 34.0 28.5 35.0 2.0 36.0 86-87 28.20778319851754 34.0 27.0 35.0 2.0 36.0 88-89 27.853289391574354 34.0 27.0 35.0 2.0 36.0 90-91 27.48170038600617 33.5 26.0 35.0 2.0 35.0 92-93 27.064655054207087 33.0 25.5 35.0 2.0 35.0 94-95 26.572112411354862 33.0 25.0 35.0 2.0 35.0 96-97 25.973220227238258 33.0 24.0 35.0 2.0 35.0 98-99 25.248600958495242 32.5 19.0 35.0 2.0 35.0 100-101 22.73060608621532 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5456602319923292 1101 2 0.47597543628002725 1101 3 0.5902841053837058 1101 4 0.5329863434780009 1101 5 0.49929815230314745 1101 6 0.5087748151589864 1101 7 0.6173541065119466 1101 8 0.4812781484280819 1101 9 0.6399728751125053 1101 10-11 0.7736134176667306 1101 12-13 0.6386799176359617 1101 14-15 0.9168063024114161 1101 16-17 0.9385458881909372 1101 18-19 1.0720648467937934 1101 20-21 0.8930594543324943 1101 22-23 0.828208673511277 1101 24-25 0.7866958980425949 1101 26-27 0.8356466558495939 1101 28-29 0.84185225064261 1101 30-31 0.584528518777077 1101 32-33 0.666674797343525 1101 34-35 0.7455266419930737 1101 36-37 0.8153270765089005 1101 38-39 0.832944337978283 1101 40-41 0.618724571596907 1101 42-43 0.7891054040677474 1101 44-45 0.7889479598912601 1101 46-47 0.7842863908730493 1101 48-49 0.9048421033027623 1101 50-51 0.755881030948224 1101 52-53 0.8454839028497148 1101 54-55 0.803414056526158 1101 56-57 0.9743733210267749 1101 58-59 0.8436538005383838 1101 60-61 0.8449236017052613 1101 62-63 0.8356164043724945 1101 64-65 0.8614772139267259 1101 66-67 0.9276730126499686 1101 68-69 0.7939449871994242 1101 70-71 0.7366475038903815 1101 72-73 0.703588028718027 1101 74-75 0.6071405597296398 1101 76-77 0.7291217786462489 1101 78-79 0.6324443584527053 1101 80-81 0.6296141042053236 1101 82-83 0.661180500132744 1101 84-85 0.8296099318896424 1101 86-87 0.6883783078665253 1101 88-89 0.8879211218553031 1101 90-91 0.6973930805526898 1101 92-93 0.9570270892663117 1101 94-95 1.1654638921165166 1101 96-97 1.0708543561348556 1101 98-99 1.1788071960350415 1101 100-101 1.6740680299934638 1102 1 0.3910761037551538 1102 2 0.553167726260952 1102 3 0.6329205547401102 1102 4 0.47123273077708205 1102 5 0.47170676577155746 1102 6 0.4375569128280006 1102 7 0.5523605753846113 1102 8 0.4728137930128682 1102 9 0.5977749933941965 1102 10-11 0.7583923795771241 1102 12-13 0.5796169173947519 1102 14-15 0.8809325660344314 1102 16-17 0.5213584224540284 1102 18-19 0.6065285882143172 1102 20-21 0.45395840834868295 1102 22-23 0.33176764989440954 1102 24-25 0.50715672298832 1102 26-27 0.4764690798129294 1102 28-29 0.2951765933393702 1102 30-31 0.3238006061397556 1102 32-33 0.5452436414985087 1102 34-35 0.2668519933953917 1102 36-37 0.20527565445333096 1102 38-39 0.058022183734045996 1102 40-41 0.36276498560475545 1102 42-43 0.31015555427715924 1102 44-45 0.3703960005903326 1102 46-47 0.40632331826817847 1102 48-49 0.7420699172623699 1102 50-51 0.735197632926166 1102 52-53 0.5214416249294018 1102 54-55 0.7019602048058715 1102 56-57 0.4834461890398529 1102 58-59 0.5099027315297704 1102 60-61 0.6114515713563762 1102 62-63 0.5917327782952668 1102 64-65 0.7916545048944243 1102 66-67 0.6141256583092485 1102 68-69 0.6513819979224067 1102 70-71 0.3335358664980994 1102 72-73 0.5096632869247237 1102 74-75 0.5664974002883802 1102 76-77 0.610061080885135 1102 78-79 0.6429041966212452 1102 80-81 0.671905729756272 1102 82-83 0.6183110617186038 1102 84-85 0.8003656935719867 1102 86-87 0.6109300040268657 1102 88-89 0.894111504067002 1102 90-91 0.9914811887911803 1102 92-93 0.8677078558825464 1102 94-95 1.0249797134440612 1102 96-97 1.1480948885559705 1102 98-99 1.217693892085741 1102 100-101 1.523499536692583 1103 1 0.5107021305402739 1103 2 0.5441773169171427 1103 3 0.6540498573187179 1103 4 0.6151426794339869 1103 5 0.6482938664974824 1103 6 0.5610840328395383 1103 7 0.6761464048551957 1103 8 0.5969309342017723 1103 9 0.7603048651336124 1103 10-11 0.7402944769643724 1103 12-13 0.6600801607115088 1103 14-15 0.8881201897011266 1103 16-17 0.9727363826577644 1103 18-19 1.101179540070838 1103 20-21 1.0429299206537408 1103 22-23 0.9426972553573734 1103 24-25 0.8196982008635487 1103 26-27 0.6948019683296067 1103 28-29 0.8036286211409163 1103 30-31 0.7980048189114086 1103 32-33 0.9161230798228885 1103 34-35 0.8548786537630448 1103 36-37 0.9812629173583787 1103 38-39 0.600549724020631 1103 40-41 0.8514518152481614 1103 42-43 0.9764622773486238 1103 44-45 1.0175045389227932 1103 46-47 1.0791274322598596 1103 48-49 1.078167760566238 1103 50-51 1.2668210296688827 1103 52-53 1.2853354988325734 1103 54-55 1.167701141538629 1103 56-57 1.2089891976979246 1103 58-59 1.2107906264834938 1103 60-61 1.2532729268507836 1103 62-63 1.3051951800393553 1103 64-65 1.4439709771199745 1103 66-67 1.414227096958804 1103 68-69 1.467500926558472 1103 70-71 1.4624774789431498 1103 72-73 1.3997403987045978 1103 74-75 1.1931157404371184 1103 76-77 1.2930714364214673 1103 78-79 1.0124098161383728 1103 80-81 1.0776327186747139 1103 82-83 1.0111629091393333 1103 84-85 1.0899326466596762 1103 86-87 0.9943256628207884 1103 88-89 1.013444054113986 1103 90-91 1.0881562098304975 1103 92-93 1.0671483072027321 1103 94-95 1.2096972446400258 1103 96-97 1.1951043401430432 1103 98-99 1.3580018483730427 1103 100-101 1.7298714586379944 1104 1 0.41825296092594044 1104 2 0.3810575476570044 1104 3 0.5106537940489986 1104 4 0.59009584576512 1104 5 0.384476861290068 1104 6 0.3624962431947907 1104 7 0.48485586190253116 1104 8 0.4448614630145258 1104 9 0.6113075173195881 1104 10-11 0.5059512911559025 1104 12-13 0.6656914589029768 1104 14-15 0.6868082111856495 1104 16-17 0.724541620717126 1104 18-19 0.7917446499173693 1104 20-21 0.7887447724501726 1104 22-23 0.5797721641139688 1104 24-25 0.5263900510581152 1104 26-27 0.312296382145 1104 28-29 0.2666714283699392 1104 30-31 0.29978661232065207 1104 32-33 0.3795840760313709 1104 34-35 0.3640138020778778 1104 36-37 0.34440878567987454 1104 38-39 0.2789657367007763 1104 40-41 0.3842492118270542 1104 42-43 0.4625083394898226 1104 44-45 0.29063296442460285 1104 46-47 0.4696471151165298 1104 48-49 0.5756889855245717 1104 50-51 0.38539596369801643 1104 52-53 0.3699462079672742 1104 54-55 0.5670212307904308 1104 56-57 0.7514233786308608 1104 58-59 0.5784647296342413 1104 60-61 0.5372399333138773 1104 62-63 0.6524114020206184 1104 64-65 0.6444521408138542 1104 66-67 0.47857320420909844 1104 68-69 0.4696571985825386 1104 70-71 0.42368556348503716 1104 72-73 0.4511298001968491 1104 74-75 0.46038888443940706 1104 76-77 0.49204912251874333 1104 78-79 0.31754773902819977 1104 80-81 0.5556397294993083 1104 82-83 0.39052262064456755 1104 84-85 0.3600506201390914 1104 86-87 0.48424939061870376 1104 88-89 0.4202278073370316 1104 90-91 0.5702796243761021 1104 92-93 0.2541221734909769 1104 94-95 0.31628091565579197 1104 96-97 0.3264719468527595 1104 98-99 0.42793938546724775 1104 100-101 0.9947872629389067 1105 1 0.4151803305435209 1105 2 0.42739344865248796 1105 3 0.5553011108200927 1105 4 0.5022747931713667 1105 5 0.48351791074561135 1105 6 0.35513672298149146 1105 7 0.4995934680716303 1105 8 0.37870992583093965 1105 9 0.45035690339241086 1105 10-11 0.6204439792478311 1105 12-13 0.5664663989835432 1105 14-15 0.8257328175020717 1105 16-17 0.9864958627497273 1105 18-19 0.7521229598598609 1105 20-21 0.7267603461136503 1105 22-23 0.7131468112151467 1105 24-25 0.6770670718194083 1105 26-27 0.6131325292862968 1105 28-29 0.8052778418158582 1105 30-31 0.5342734372313274 1105 32-33 0.6562706839025338 1105 34-35 0.537367380055791 1105 36-37 0.5736430027026245 1105 38-39 0.4875786541455014 1105 40-41 0.31375014670202006 1105 42-43 0.45710840345179804 1105 44-45 0.5931993189871605 1105 46-47 0.8983968860392224 1105 48-49 0.8758913521177334 1105 50-51 0.8898503943825204 1105 52-53 0.8104359662652598 1105 54-55 0.5732461445794996 1105 56-57 0.6288114721207521 1105 58-59 0.7628351293726396 1105 60-61 0.7798329506782338 1105 62-63 0.9293598766951519 1105 64-65 0.9348884776850639 1105 66-67 0.8732013862875263 1105 68-69 0.966591006320499 1105 70-71 0.9898490785670049 1105 72-73 0.7907315171088065 1105 74-75 0.7904906306972102 1105 76-77 0.8561892112270577 1105 78-79 1.1006595910388697 1105 80-81 1.1687299554144488 1105 82-83 0.9364991846885928 1105 84-85 1.0938067932484685 1105 86-87 1.1842876300592877 1105 88-89 1.030561918834497 1105 90-91 1.0639097780166047 1105 92-93 1.2329617187620414 1105 94-95 1.2671550626797021 1105 96-97 1.32837089291748 1105 98-99 1.278886867174002 1105 100-101 1.5642289790842732 1106 1 0.26538222751005947 1106 2 0.08354607092306665 1106 3 0.10554261327949988 1106 4 0.28158854834113356 1106 5 0.3221221130249745 1106 6 0.2369399337578244 1106 7 0.44437339582574964 1106 8 0.5519646096357107 1106 9 0.42307691304340267 1106 10-11 0.5458385546610103 1106 12-13 0.5342604754585594 1106 14-15 0.5395350472457991 1106 16-17 0.5433934779983005 1106 18-19 0.6691027906488074 1106 20-21 0.5487461746309705 1106 22-23 0.2227269588480354 1106 24-25 -0.041352953537213466 1106 26-27 0.009003372132156073 1106 28-29 0.08939347847415746 1106 30-31 0.12902445752800418 1106 32-33 0.3303052431093292 1106 34-35 0.21866863673353265 1106 36-37 0.044665552795599694 1106 38-39 0.21497023410876892 1106 40-41 0.054508580509633475 1106 42-43 -0.08875657823634953 1106 44-45 -0.10547543342160282 1106 46-47 0.04312138886509587 1106 48-49 0.02306331507066517 1106 50-51 -0.002655688702525083 1106 52-53 0.15159261737046137 1106 54-55 0.32520877884553556 1106 56-57 0.24487064591374974 1106 58-59 0.20154506010855044 1106 60-61 0.4578804112605326 1106 62-63 0.40868876844695734 1106 64-65 0.1180305931191441 1106 66-67 0.08476044285737316 1106 68-69 0.32092217425006453 1106 70-71 -0.13207893503543033 1106 72-73 -0.12847640717641795 1106 74-75 -0.0917077814411904 1106 76-77 -0.11795340951848132 1106 78-79 -0.006182200950348715 1106 80-81 0.024687167825078404 1106 82-83 0.2372693294307382 1106 84-85 0.4957211725461761 1106 86-87 0.5171629774609166 1106 88-89 0.5242586135967819 1106 90-91 0.5762039830956631 1106 92-93 0.442754745919121 1106 94-95 0.7200478569837969 1106 96-97 0.8715474241591608 1106 98-99 1.0398742617016197 1106 100-101 1.7242587856319247 1107 1 0.25864839021858543 1107 2 0.20894973711147102 1107 3 0.2816358312592442 1107 4 0.3235318209282383 1107 5 0.3616123372363802 1107 6 0.3317591940957456 1107 7 0.3359419120045146 1107 8 0.4246769894459703 1107 9 0.376348536488166 1107 10-11 0.4991803110907611 1107 12-13 0.3882767934233087 1107 14-15 0.13729320087450958 1107 16-17 0.2613381532450987 1107 18-19 0.35394630308001496 1107 20-21 0.18560480580642036 1107 22-23 0.3396126795581438 1107 24-25 0.14505757604712244 1107 26-27 0.17377653743185562 1107 28-29 0.17412108410192673 1107 30-31 0.1634873245215971 1107 32-33 0.2772001185831314 1107 34-35 0.2881160851777551 1107 36-37 0.4363997648434932 1107 38-39 0.33295693560993556 1107 40-41 0.4087692931892519 1107 42-43 0.2991709117522987 1107 44-45 0.28274677571388906 1107 46-47 0.2050378396006849 1107 48-49 0.49674497556277686 1107 50-51 0.3991696426892162 1107 52-53 0.45690050652822123 1107 54-55 0.5833838222102088 1107 56-57 0.5969419781192471 1107 58-59 0.5871439743857039 1107 60-61 0.7732462270516933 1107 62-63 0.7462928971918501 1107 64-65 0.7483347347128131 1107 66-67 0.7567420476713806 1107 68-69 0.7521177007292863 1107 70-71 0.6302227117513013 1107 72-73 0.6465396538125106 1107 74-75 0.713379536801952 1107 76-77 0.6395085259377602 1107 78-79 0.8958513879311631 1107 80-81 0.7958178079090246 1107 82-83 0.8959468140657236 1107 84-85 0.8973096043512179 1107 86-87 1.020902243366649 1107 88-89 0.9977472745386002 1107 90-91 0.8069578323793465 1107 92-93 0.8063027254467094 1107 94-95 1.074156257951735 1107 96-97 1.2285861525571313 1107 98-99 1.354909488804001 1107 100-101 1.7716429859017495 1108 1 0.24542810210333954 1108 2 0.2231003973755783 1108 3 0.4309755671536024 1108 4 0.3416140538213952 1108 5 0.2929748822543843 1108 6 0.3842351844919065 1108 7 0.40948632977162447 1108 8 0.41160676135474006 1108 9 0.10817626758060328 1108 10-11 0.2033519797582315 1108 12-13 0.2756942604101056 1108 14-15 0.6742980027952825 1108 16-17 0.8364281892595073 1108 18-19 0.6958730737882988 1108 20-21 0.6392137493838561 1108 22-23 0.663434708369671 1108 24-25 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-7.693674851323269 2316 100-101 -8.302349451615541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4674.0 3 1779.0 4 1785.0 5 1983.0 6 2254.0 7 2689.0 8 3307.0 9 4144.0 10 5402.0 11 6636.0 12 8549.0 13 10543.0 14 11524.0 15 13812.0 16 16649.0 17 20511.0 18 16605.0 19 12546.0 20 12850.0 21 13799.0 22 15095.0 23 17314.0 24 19935.0 25 22574.0 26 26078.0 27 30577.0 28 36144.0 29 43405.0 30 52804.0 31 63682.0 32 78606.0 33 99107.0 34 128932.0 35 173456.0 36 234413.0 37 300254.0 38 236405.0 39 35929.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.33431913719757 18.535577157356144 14.05593876813725 22.074164937309035 2 15.443542269327171 20.708977889649237 39.94021869053291 23.907261150490687 3 20.091511302328758 25.419072280942164 29.49381061616192 24.99560580056716 4 12.458154287799973 16.55998575846263 37.79359175603082 33.18826819770658 5 13.350561260564747 38.091260615133294 33.61018940829572 14.947988716006241 6 30.74841266242182 36.09707267013667 17.953584407281067 15.200930260160444 7 28.26497924423356 33.3231015517393 20.71980583514023 17.692113368886908 8 26.603609809093403 33.40949436912342 19.846165907856346 20.14072991392683 9 26.765302876192436 17.15336929880767 20.825398674594602 35.25592915040529 10-11 25.079105682952264 26.60748348161332 26.897528318434393 21.41588251700002 12-13 26.847417863376954 24.211319043266343 27.35725214134036 21.58401095201634 14-15 23.173017563948758 25.339345233012438 26.85205379353016 24.635583409508637 16-17 21.789349304616383 28.71039729406593 27.129929075631193 22.370324325686493 18-19 21.575811164508675 27.3949946409552 29.620734183538044 21.40846001099808 20-21 22.944218756385087 25.971974749060816 29.335939708540526 21.747866786013574 22-23 22.215371474119287 26.22065217878046 29.462374559474817 22.10160178762544 24-25 22.428929748333648 26.269632961963286 29.61868115535151 21.682756134351557 26-27 22.129213388793406 26.144507829163565 30.09906117216322 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22.570367030986034 27.722412091406973 27.575581787445575 22.131639090161418 54-55 21.9785247617819 27.140115141663202 27.924749636981666 22.95661045957323 56-57 22.533555830863445 27.364705559705072 27.550720285847575 22.551018323583907 58-59 23.20558242102671 26.96839948955605 27.518889784404593 22.30712830501265 60-61 22.48628096943991 27.153983647145054 28.448689608350325 21.911045775064707 62-63 22.2746370837524 27.366266545102246 28.279231009896975 22.079865361248373 64-65 21.98453727971864 27.869583248682382 27.84140357711128 22.304475894487698 66-67 22.267733070062544 27.45062483437139 27.447350739763486 22.834291355802577 68-69 22.09738854870337 27.4364837519243 27.820042497615 22.64608520175733 70-71 22.51547046067594 27.414190819278883 27.539980573248673 22.5303581467965 72-73 22.414383307624462 27.183525499083743 28.021967717945294 22.380123475346497 74-75 21.974137405041645 28.216493016002207 27.44411934119291 22.36525023776324 76-77 22.25722676674825 27.545351334284284 27.37580866562614 22.82161323334132 78-79 22.31144723657473 27.62350464909285 27.4775942273997 22.587453886932717 80-81 22.60756641781124 27.664821824644388 27.29026525143261 22.43734650611177 82-83 22.356354697421146 27.563370108523333 27.664172356732497 22.416102837323027 84-85 22.249006750764615 27.79614666590921 27.395041043854086 22.559805539472087 86-87 22.32156249510279 27.862208769977816 27.277230160986647 22.538998573932744 88-89 22.092976073309405 27.85409198991341 26.91637257703115 23.13655935974604 90-91 22.248000143286685 27.412841397313827 27.217053578025197 23.122104881374298 92-93 22.615474302365644 27.610533230006006 27.363269710904586 22.410722756723764 94-95 22.210193673014214 27.489230981077146 27.48226201034934 22.8183133355593 96-97 21.760325233196017 27.51104303389777 27.839332427855396 22.889299305050812 98-99 21.987316371632495 27.852983664447294 27.07952137314818 23.08017859077203 100-101 21.84084088622879 27.363928927491642 27.425309323046733 23.369920863232835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 640.0 1 447.5 2 438.5 3 643.0 4 566.5 5 530.5 6 783.0 7 1391.0 8 2155.0 9 2580.5 10 2396.0 11 2107.0 12 2178.5 13 2286.0 14 2355.0 15 2490.0 16 2671.0 17 2867.5 18 3054.5 19 3289.5 20 3690.5 21 4218.5 22 4897.0 23 5736.0 24 6803.0 25 8255.0 26 10187.5 27 12684.5 28 15285.5 29 18092.5 30 21595.5 31 25233.5 32 29142.0 33 32275.0 34 35226.0 35 38257.0 36 41484.5 37 45345.5 38 47363.5 39 48128.5 40 49151.0 41 51414.5 42 53945.5 43 56413.0 44 58691.0 45 61201.0 46 63283.0 47 69568.5 48 85422.0 49 85442.0 50 69621.5 51 60871.0 52 57115.0 53 56080.5 54 54908.5 55 51891.5 56 47775.5 57 43183.0 58 39851.0 59 35728.0 60 30266.0 61 25865.5 62 21921.5 63 17135.0 64 12543.0 65 9535.0 66 7095.0 67 5526.0 68 4154.5 69 2742.0 70 2034.0 71 1608.0 72 1280.0 73 1119.5 74 908.5 75 693.5 76 490.0 77 274.5 78 172.0 79 113.5 80 78.0 81 60.0 82 40.0 83 25.0 84 15.5 85 10.0 86 8.5 87 9.5 88 7.0 89 3.5 90 3.0 91 1.5 92 1.5 93 4.0 94 4.0 95 1.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07913770127924359 2 0.03257294352512006 3 0.017517751414712335 4 0.024345756758466026 5 0.028655235540999097 6 0.0331326160942802 7 0.0823838021803724 8 0.05982899764321881 9 0.07516402603820661 10-11 0.06564959236248426 12-13 0.0440182475644449 14-15 0.04793595554856587 16-17 0.0589894887894786 18-19 0.055575486117601755 20-21 0.020707885058925127 22-23 0.014271650513583532 24-25 0.024933412956084176 26-27 0.027367988631930774 28-29 0.006772038086837676 30-31 0.0013432141659843325 32-33 0.01001813898796648 34-35 0.01175312395236291 36-37 0.00472923320940317 38-39 0.010186040758714522 40-41 0.004309478782533066 42-43 0.002266673905098561 44-45 3.3580354149608313E-4 46-47 4.477380553281109E-4 48-49 7.835415968241939E-4 50-51 0.001119345138320277 52-53 1.3991814229003462E-4 54-55 1.9588539920604848E-4 56-57 0.004057626126411004 58-59 0.004421413296365095 60-61 0.005512774806227365 62-63 0.0029102973596327204 64-65 5.596725691601385E-4 66-67 3.078199130380762E-4 68-69 0.0039456916125789766 70-71 0.0026584447035106584 72-73 0.022974558964023686 74-75 0.017797587699292405 76-77 0.027032185090434695 78-79 0.02177126294032939 80-81 0.013795928829797414 82-83 0.005176971264731282 84-85 0.004057626126411004 86-87 0.0015111159367323741 88-89 0.0017070013359384224 90-91 0.007331710655997816 92-93 0.01138933678240882 94-95 0.01502720848194972 96-97 0.012620616434561124 98-99 0.024653576671504106 100-101 0.02003627797593296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1786759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.720902287029375 #Duplication Level Percentage of deduplicated Percentage of total 1 80.21133013608686 48.705043395059846 2 12.016785070926622 14.593400641919374 3 3.5561070507396515 6.477900862505356 4 1.4322978597085252 3.4788167354113066 5 0.7534425802007121 2.2874856645627366 6 0.45818067422200104 1.6692686369543617 7 0.2896326503691945 1.231072910354084 8 0.20273364107871888 0.9848135688187659 9 0.14968344700981812 0.8180022563882015 >10 0.8317645736931414 9.289717427879857 >50 0.06331773075983195 2.6414533826631987 >100 0.03045421391478393 3.3779887752964033 >500 0.00241368812057869 0.9849484436399313 >1k 0.0014853465357407324 1.8274505576811613 >5k 3.713366339351831E-4 1.6326367408655256 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9181 0.5138353857459232 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7771 0.4349215534943437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6299 0.35253775131397125 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5712 0.31968497150427116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3701 0.20713481784616727 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3593 0.20109035409923778 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3557 0.1990755328502613 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2635 0.14747372197369651 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2270 0.12704567319935145 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2104 0.11775510855129316 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2013 0.11266208817193588 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1790 0.10018138987966481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.5967256916013855E-5 0.0 0.0 0.0 0.0 6 5.5967256916013855E-5 0.0 0.0 0.0 0.0 7 5.5967256916013855E-5 0.0 0.0 0.0 0.0 8 5.5967256916013855E-5 0.0 0.0 0.0 0.0 9 5.5967256916013855E-5 0.0 0.0 0.0 0.0 10-11 5.5967256916013855E-5 0.0 0.0 0.0 0.0 12-13 5.5967256916013855E-5 0.0 0.0 0.0 0.0 14-15 5.5967256916013855E-5 0.0 0.0 0.0 0.0 16-17 5.5967256916013855E-5 0.0 0.0 0.0 0.0 18-19 5.5967256916013855E-5 0.0 0.0 0.0 0.0 20-21 5.5967256916013855E-5 0.0 0.0 2.7983628458006928E-5 0.0 22-23 5.5967256916013855E-5 0.0 0.0 8.395088537402078E-5 0.0 24-25 5.5967256916013855E-5 0.0 0.0 3.3580354149608313E-4 0.0 26-27 5.5967256916013855E-5 0.0 0.0 6.436234545341593E-4 0.0 28-29 1.1193451383202771E-4 0.0 0.0 0.0010353942529462562 0.0 30-31 1.6790177074804157E-4 0.0 0.0 0.002322641162014575 0.0 32-33 1.6790177074804157E-4 0.0 0.0 0.004169560640243032 0.0 34-35 1.958853992060485E-4 0.0 0.0 0.005736643833891421 0.0 36-37 2.798362845800693E-4 0.0 0.0 0.0084790394227761 0.0 38-39 2.798362845800693E-4 0.0 0.0 0.012732550948393151 0.0 40-41 2.798362845800693E-4 0.0 0.0 0.0214074757703753 0.0 42-43 3.6378716995409005E-4 0.0 0.0 0.03005441696389944 0.0 44-45 5.037053122441247E-4 0.0 0.0 0.03875732541433959 0.0 46-47 6.716070829921663E-4 0.0 0.0 0.057954094536532344 0.0 48-49 6.716070829921663E-4 0.0 0.0 0.07099446539796359 0.0 50-51 6.716070829921663E-4 0.0 0.0 0.08744883893127164 0.0 52-53 6.716070829921663E-4 0.0 0.0 0.10359539255154165 0.0 54-55 7.275743399081801E-4 0.0 0.0 0.12178475104924616 0.0 56-57 8.395088537402079E-4 0.0 0.0 0.17333059466889492 0.0 58-59 8.395088537402079E-4 0.0 0.0 0.22599578342686394 0.0 60-61 8.395088537402079E-4 0.0 0.0 0.26430537078587546 0.0 62-63 8.395088537402079E-4 0.0 0.0 0.31064625951233493 0.0 64-65 8.395088537402079E-4 0.0 0.0 0.3606250199383353 0.0 66-67 9.514433675722355E-4 0.0 0.0 0.4411898862689372 0.0 68-69 9.514433675722355E-4 0.0 0.0 0.5405037836664037 0.0 70-71 0.0010074106244882493 0.0 0.0 0.5916858401160985 0.0 72-73 0.0010074106244882493 0.0 0.0 0.6466177027791661 0.0 74-75 0.0010074106244882493 0.0 0.0 0.7495974555046316 0.0 76-77 0.0010074106244882493 0.0 0.0 0.8777904574707613 0.0 78-79 0.0010074106244882493 0.0 0.0 1.0031571129626322 0.0 80-81 0.0012872469090683186 0.0 0.0 1.0996726475142982 0.0 82-83 0.0012872469090683186 0.0 0.0 1.2316994065791749 0.0 84-85 0.0012872469090683186 0.0 0.0 1.478403075064964 0.0 86-87 0.0012872469090683186 0.0 0.0 1.7404417719457408 0.0 88-89 0.0012872469090683186 0.0 0.0 2.0461349292210085 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1860 0.0 32.708893 2 TATCACG 155 1.3478711E-9 30.664589 2 GTACCTG 2100 0.0 29.876072 1 GTACATG 9990 0.0 25.54922 1 TACATGG 10035 0.0 25.055737 2 GTGTAGC 950 0.0 24.515533 1 ACATGGG 10130 0.0 23.696667 3 GAGTACT 7170 0.0 22.437994 12-13 ACCTGGG 2700 0.0 22.182583 3 CATGGGA 6415 0.0 20.747545 4 AGTACTT 7400 0.0 20.327618 12-13 GTACTTT 7960 0.0 20.027779 14-15 GACCGTA 120 0.00474554 19.805323 7 CATGGGG 3950 0.0 19.25432 4 ATGGGAT 2595 0.0 18.867102 5 AGAGTAC 13895 0.0 18.337065 10-11 GTGTAAG 795 0.0 17.935892 1 CATGGGT 1330 0.0 17.512566 4 ACTTTTT 9475 0.0 17.174816 16-17 ATGGGAG 2755 0.0 17.081223 5 >>END_MODULE