FastQCFastQC Report
Thu 26 May 2016
SRR936484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786759
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108280.606013457886598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96320.5390766186150454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83180.4655356430274033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53070.29701823245328557No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50750.2840338288487703No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33710.1886656230638827No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33690.1885536885500507No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27730.15519720342810642No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25510.14277247239275134No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25080.14036588034536276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.12296006344448243No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21460.12010573334176573No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20510.11478884393474442No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19280.10790487133407471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15900.028.3828872
GTACCTG16350.028.1914751
GTACATG98350.023.8680211
TACATGG99800.023.0856062
TATCACG1050.002195623222.6209472
GAGTACT99350.022.27545412-13
ACATGGG101750.022.1271443
AGTACTT101150.021.01046612-13
GTACTTT107050.020.583914-15
CTGGTCG2953.0267984E-919.3182499
CATGGGG37600.019.1983664
CATGGGA67600.019.1789344
TACTTTT112750.018.70091214-15
AGAGTAC166300.018.60504310-11
ACTTTTT122500.018.20102716-17
TCGCGCT2250.017.94620160-61
TATAACG1901.8652098E-417.5014692
ATAACGC2453.7650625E-617.448493
CTCGCGC2053.274181E-1117.37975160-61
GTATCAA242350.017.3133661
Produced by FastQC (version 0.11.5)