##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1786759 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85179534565098 31.0 31.0 34.0 30.0 34.0 2 31.995979871935724 31.0 31.0 34.0 30.0 34.0 3 32.074579168203435 33.0 31.0 34.0 30.0 34.0 4 35.78584688813657 37.0 35.0 37.0 35.0 37.0 5 35.62107144835985 37.0 35.0 37.0 33.0 37.0 6 35.713961424008495 37.0 35.0 37.0 33.0 37.0 7 35.65890867207049 37.0 35.0 37.0 33.0 37.0 8 35.63282960936534 37.0 35.0 37.0 33.0 37.0 9 37.248866802965594 39.0 37.0 39.0 33.0 39.0 10-11 37.13712649551506 39.0 37.0 39.0 33.0 39.0 12-13 37.03047948828018 39.0 37.0 39.0 33.0 39.0 14-15 38.12078881371242 40.0 37.0 41.0 33.0 41.0 16-17 38.03103216494222 40.0 37.0 41.0 33.0 41.0 18-19 38.20816545488228 40.0 37.5 41.0 33.5 41.0 20-21 38.238270242377396 40.0 38.0 41.0 33.0 41.0 22-23 38.1448821581422 40.0 37.0 41.0 33.0 41.0 24-25 38.0008965954558 40.0 37.0 41.0 33.0 41.0 26-27 37.799549351647315 40.0 37.0 41.0 33.0 41.0 28-29 37.70904134245301 40.0 37.0 41.0 32.5 41.0 30-31 37.655052807905264 39.5 37.0 41.0 33.0 41.0 32-33 37.468395569855815 39.0 36.0 41.0 32.0 41.0 34-35 37.2666422835984 39.0 36.0 41.0 31.5 41.0 36-37 37.04500047292332 39.0 36.0 41.0 31.0 41.0 38-39 36.86930470197716 39.0 35.0 41.0 31.0 41.0 40-41 36.67298051947689 39.0 35.0 41.0 30.5 41.0 42-43 36.36965365782403 39.0 35.0 40.0 30.0 41.0 44-45 36.06369829395011 39.0 35.0 40.0 30.0 41.0 46-47 35.64065103351935 38.5 35.0 40.0 28.5 41.0 48-49 35.21003476126327 38.0 34.5 40.0 26.5 41.0 50-51 34.90458813975472 38.0 34.0 40.0 26.0 41.0 52-53 34.55312356059211 38.0 34.0 40.0 24.0 41.0 54-55 34.370365561331994 38.0 34.0 40.0 24.0 41.0 56-57 34.05786230823519 38.0 33.0 40.0 23.5 41.0 58-59 33.688418807460884 37.0 33.0 40.0 23.0 41.0 60-61 33.25383305750803 36.5 32.0 39.5 22.0 41.0 62-63 32.94487113259259 36.0 32.0 39.0 21.5 40.0 64-65 32.530567916546104 36.0 31.0 39.0 20.0 40.0 66-67 32.10087118632116 35.0 31.0 38.5 18.5 40.0 68-69 31.734010854289807 35.0 31.0 38.0 18.0 40.0 70-71 31.356815048923778 35.0 30.5 37.5 16.0 39.5 72-73 30.898382770144156 34.5 30.0 37.0 12.5 39.0 74-75 30.40719593409072 34.0 29.5 36.0 9.0 39.0 76-77 28.92444476283595 32.5 28.0 35.0 7.5 37.0 78-79 29.685400493295404 34.0 29.0 35.0 7.0 37.0 80-81 29.448894338855993 34.0 29.0 35.0 4.0 37.0 82-83 29.148307354265462 34.0 29.0 35.0 2.0 36.5 84-85 28.768771837724056 34.0 29.0 35.0 2.0 36.0 86-87 28.397777484260608 33.0 28.5 35.0 2.0 36.0 88-89 27.920025588229862 33.0 27.0 35.0 2.0 35.0 90-91 27.44084540780262 33.0 26.0 35.0 2.0 35.0 92-93 26.845699671863976 32.5 25.5 34.5 2.0 35.0 94-95 26.291903384843728 32.0 25.0 34.0 2.0 35.0 96-97 25.543912189612588 32.0 23.5 34.0 2.0 35.0 98-99 24.662150015754783 32.0 13.0 34.0 2.0 35.0 100-101 21.439750128584773 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3144593088676295 1101 2 0.31860549422298945 1101 3 0.33462000208579923 1101 4 0.2203897428607462 1101 5 0.27337192276673505 1101 6 0.2558365715850428 1101 7 0.283846949676132 1101 8 0.20845266644840166 1101 9 0.26064828927293604 1101 10-11 0.33970779043126953 1101 12-13 0.315481130673426 1101 14-15 0.4767740085286931 1101 16-17 0.5820123354761577 1101 18-19 0.43623589611167546 1101 20-21 0.43258796423287293 1101 22-23 0.2942020203903155 1101 24-25 0.25606902827650657 1101 26-27 0.23420241624496185 1101 28-29 0.38143138614838534 1101 30-31 0.4049294206403289 1101 32-33 0.36281749638939687 1101 34-35 0.43203856067313495 1101 36-37 0.3940620988180754 1101 38-39 0.32586638689532066 1101 40-41 0.457775801060059 1101 42-43 0.3946770171209195 1101 44-45 0.12190164120387692 1101 46-47 0.337958195260434 1101 48-49 0.3807141700415215 1101 50-51 0.32325124491381274 1101 52-53 0.339345568435661 1101 54-55 0.32848244821598627 1101 56-57 0.4187477634004537 1101 58-59 0.2512064641692362 1101 60-61 0.4609346666470415 1101 62-63 0.414244995912874 1101 64-65 0.3656088025535098 1101 66-67 0.08398898909452157 1101 68-69 0.28330467484982336 1101 70-71 0.3371753906265518 1101 72-73 0.38499856044533587 1101 74-75 0.29466819551157997 1101 76-77 0.19566174935270908 1101 78-79 0.1509619851151065 1101 80-81 0.2421754389604871 1101 82-83 0.3305840576810546 1101 84-85 0.4752575362860476 1101 86-87 0.2613702255230841 1101 88-89 0.5185127632426969 1101 90-91 0.6237747561980704 1101 92-93 0.49398017305227526 1101 94-95 0.3995678686177975 1101 96-97 0.6134279032003747 1101 98-99 0.6281892922642669 1101 100-101 1.0076509137171712 1102 1 0.3214445949233671 1102 2 0.266945515712802 1102 3 0.24638677181585678 1102 4 0.16054061012825116 1102 5 0.3097193121928683 1102 6 0.33138313650401585 1102 7 0.31175191206895647 1102 8 0.4197633258348432 1102 9 0.4926812695593199 1102 10-11 0.36364974600436284 1102 12-13 0.4430335943271331 1102 14-15 0.5350706118223343 1102 16-17 0.5593712811595637 1102 18-19 0.5351559233028738 1102 20-21 0.5279166706776266 1102 22-23 0.4865632685538088 1102 24-25 0.47891566117345974 1102 26-27 0.5028925243518927 1102 28-29 0.47705941500613847 1102 30-31 0.5782135247562863 1102 32-33 0.5987816873417486 1102 34-35 0.4638018218620914 1102 36-37 0.5676789661294421 1102 38-39 0.5295552889412392 1102 40-41 0.5660565062768299 1102 42-43 0.6244711096148379 1102 44-45 0.6192691389562199 1102 46-47 0.7005061635958469 1102 48-49 0.7057750213013065 1102 50-51 0.5734612091705529 1102 52-53 0.7023183991704798 1102 54-55 0.6006876759422326 1102 56-57 0.6239447899118673 1102 58-59 0.6507097863190907 1102 60-61 0.7206560665549446 1102 62-63 0.787580631726307 1102 64-65 0.7389998075312505 1102 66-67 0.5953835245417523 1102 68-69 0.6115862572018642 1102 70-71 0.5937286875587695 1102 72-73 0.7932748799857201 1102 74-75 0.6818351582113991 1102 76-77 0.7059439676278245 1102 78-79 0.7288324103064312 1102 80-81 0.7847724172294583 1102 82-83 0.8490285679106435 1102 84-85 0.7820092850745013 1102 86-87 0.8700357738422717 1102 88-89 0.5506082394175102 1102 90-91 0.5446768898296277 1102 92-93 0.8417199050874515 1102 94-95 0.5090760150116758 1102 96-97 0.6862230043998601 1102 98-99 0.9407537068861132 1102 100-101 1.688279929352106 1103 1 0.2787687824171705 1103 2 0.2673453394222456 1103 3 0.2589019697183659 1103 4 0.254848899772405 1103 5 0.28501716040506153 1103 6 0.27449261968841654 1103 7 0.19691865696199784 1103 8 0.18858443898086108 1103 9 0.33692283670465173 1103 10-11 0.38477751473241995 1103 12-13 0.40551663249648584 1103 14-15 0.49382128032553396 1103 16-17 0.4885881686495779 1103 18-19 0.4081254886403016 1103 20-21 0.3163930560258805 1103 22-23 0.27134972076771646 1103 24-25 0.4432515908044863 1103 26-27 0.3672750830395657 1103 28-29 0.36695399857082833 1103 30-31 0.3250039424851465 1103 32-33 0.3675782588790071 1103 34-35 0.45797728004881577 1103 36-37 0.43573356302839983 1103 38-39 0.524777344485031 1103 40-41 0.6225666281565978 1103 42-43 0.639083081216107 1103 44-45 0.6569844787910313 1103 46-47 0.6823274781875668 1103 48-49 0.5254560621927382 1103 50-51 0.42761860882348657 1103 52-53 0.3923200455034035 1103 54-55 0.3004088219126544 1103 56-57 0.16806651220106517 1103 58-59 0.3857008030677278 1103 60-61 0.28913942581035457 1103 62-63 0.34522273531030123 1103 64-65 0.4712667057070945 1103 66-67 0.4678484530489797 1103 68-69 0.4503151654786208 1103 70-71 0.27721249163083783 1103 72-73 0.3244135799553476 1103 74-75 0.3965824154309807 1103 76-77 0.442696867372284 1103 78-79 0.3247849877377611 1103 80-81 0.4805525898592258 1103 82-83 0.5395890471264586 1103 84-85 0.7202712892445327 1103 86-87 0.593950344502165 1103 88-89 0.4986925112117362 1103 90-91 0.4648737135319152 1103 92-93 0.39743600366699994 1103 94-95 0.43717421416887703 1103 96-97 0.6284234254929615 1103 98-99 0.5448479630248357 1103 100-101 0.9852287938425448 1104 1 0.1940793946261259 1104 2 0.19832238210295472 1104 3 0.18300265678172423 1104 4 0.16559360676514245 1104 5 0.06919000294888633 1104 6 0.10630149445011483 1104 7 0.09137672314552958 1104 8 0.11799585587379369 1104 9 0.18657285178870353 1104 10-11 0.1888875287928542 1104 12-13 0.25758851967479757 1104 14-15 0.2813973323863266 1104 16-17 0.303137012518377 1104 18-19 0.22311000563441752 1104 20-21 0.13623832451401796 1104 22-23 0.11311899552853077 1104 24-25 0.10076689658470883 1104 26-27 0.07398314580574095 1104 28-29 0.12601166033017108 1104 30-31 0.1810382139541602 1104 32-33 0.18210071758224444 1104 34-35 0.20084509958826402 1104 36-37 0.17954075804052394 1104 38-39 0.18566709041496665 1104 40-41 0.22118817809751334 1104 42-43 0.20466945019452254 1104 44-45 0.08777650741925669 1104 46-47 0.17465352762574327 1104 48-49 0.10027262966072215 1104 50-51 0.054847082422590177 1104 52-53 0.2356449324048242 1104 54-55 0.16537002141406987 1104 56-57 -0.02387857359364176 1104 58-59 0.12107475874763196 1104 60-61 0.1748317359968965 1104 62-63 -0.049029335845723665 1104 64-65 0.07038448749668902 1104 66-67 -0.0401247943005103 1104 68-69 -0.02645444465989044 1104 70-71 0.08415509626630069 1104 72-73 0.12161242905349923 1104 74-75 -0.12329149526699723 1104 76-77 -0.2753364713970292 1104 78-79 -0.2679040325683246 1104 80-81 -0.02528234645238925 1104 82-83 -0.1536813369239347 1104 84-85 0.10096122635564697 1104 86-87 0.12427643653807152 1104 88-89 0.06784469226356649 1104 90-91 0.11071533196618333 1104 92-93 0.32566088284360717 1104 94-95 0.16024711956025328 1104 96-97 0.5151326771810538 1104 98-99 0.6165892281839902 1104 100-101 1.0224256783584913 1105 1 0.020877187339014114 1105 2 0.1358519525395785 1105 3 0.06076820379437464 1105 4 0.15082545695517524 1105 5 0.2105950822964715 1105 6 0.19533471036011463 1105 7 0.10782327596626828 1105 8 0.06550121897779349 1105 9 0.0860627130966165 1105 10-11 0.11806535985896716 1105 12-13 0.15584226186969374 1105 14-15 0.23871798431937918 1105 16-17 0.24496994239616754 1105 18-19 0.11290765101914957 1105 20-21 0.2195058293141514 1105 22-23 0.24642611722757124 1105 24-25 0.18005204962033616 1105 26-27 0.016218832516628368 1105 28-29 0.14394377199771924 1105 30-31 0.11993236646394934 1105 32-33 0.12269872613686061 1105 34-35 0.26522795035204183 1105 36-37 0.2991637297331593 1105 38-39 0.2932721874898476 1105 40-41 0.2745649872093381 1105 42-43 0.32097050149466355 1105 44-45 0.3011121604722433 1105 46-47 0.1976948118480948 1105 48-49 0.1363982706280069 1105 50-51 0.2508616463492359 1105 52-53 0.33950424120821765 1105 54-55 0.22937556837880635 1105 56-57 0.15850633976423723 1105 58-59 0.14427909211170942 1105 60-61 0.41319625578498886 1105 62-63 0.1629113412169616 1105 64-65 0.26323808404034565 1105 66-67 0.23316817551159374 1105 68-69 0.23274697687708823 1105 70-71 0.21838706144415454 1105 72-73 0.5743681541894752 1105 74-75 0.47514541893890794 1105 76-77 0.4044952031970084 1105 78-79 0.24472621210420797 1105 80-81 0.18651878809414768 1105 82-83 0.5035675075083184 1105 84-85 0.5261057995891143 1105 86-87 0.7824616531763304 1105 88-89 0.7701543904085462 1105 90-91 0.8155123904338986 1105 92-93 0.6284858102874864 1105 94-95 0.8627062419875529 1105 96-97 1.0237402944057514 1105 98-99 0.8992318460255504 1105 100-101 1.348953369462869 1106 1 0.22294259103052028 1106 2 0.2178210966739833 1106 3 0.20389312270554427 1106 4 0.08379316026906025 1106 5 0.1693735032209176 1106 6 0.0799546911036515 1106 7 0.06880219173175561 1106 8 0.12618354878228644 1106 9 0.10615111984047587 1106 10-11 0.18783618290208182 1106 12-13 0.26060277382759267 1106 14-15 0.28057746220972746 1106 16-17 0.2601275790863298 1106 18-19 0.33359446489381384 1106 20-21 0.21606282594313342 1106 22-23 0.11466501573963228 1106 24-25 0.12621560892500838 1106 26-27 0.09518171488603144 1106 28-29 -0.15256878226066561 1106 30-31 0.05712294855089084 1106 32-33 0.08841664398050142 1106 34-35 0.058902395476806646 1106 36-37 -0.03552089445851436 1106 38-39 0.3745246201548156 1106 40-41 0.3409701969249781 1106 42-43 0.22939389559434886 1106 44-45 0.13561013808831035 1106 46-47 0.03725443973855391 1106 48-49 -0.15813002567570322 1106 50-51 0.028118590398513277 1106 52-53 -0.41223618120484673 1106 54-55 -0.12679361308490655 1106 56-57 -0.2626112976543098 1106 58-59 -0.10794786329568495 1106 60-61 -0.1671394462906548 1106 62-63 -0.07372379659422279 1106 64-65 -0.06017462340480506 1106 66-67 -0.08472935324222064 1106 68-69 -0.06439038817402576 1106 70-71 0.10475600026732579 1106 72-73 0.14504318884428713 1106 74-75 0.1689861388484104 1106 76-77 0.04827338512279056 1106 78-79 -0.1205215043204646 1106 80-81 0.141460995941042 1106 82-83 0.05839778431429821 1106 84-85 0.24195658930898745 1106 86-87 0.5182617182750171 1106 88-89 0.492255848755633 1106 90-91 0.3124045542367533 1106 92-93 0.46350104929753044 1106 94-95 0.3756719195032403 1106 96-97 0.6547616381245263 1106 98-99 0.6525098265096005 1106 100-101 1.4156118039533325 1107 1 0.010787099243039222 1107 2 0.013642027354759279 1107 3 -0.024614061910170904 1107 4 0.04415517495951349 1107 5 -0.02288028181880719 1107 6 -0.08763155640689035 1107 7 0.01974390104927437 1107 8 0.06596715372479167 1107 9 0.05914917110715123 1107 10-11 0.0302942101843513 1107 12-13 -0.13306384287155026 1107 14-15 -0.0055137213780867 1107 16-17 0.12171692101407672 1107 18-19 -0.047132080708685464 1107 20-21 -0.05928971276384942 1107 22-23 -0.09042257018666078 1107 24-25 0.04801599224727937 1107 26-27 -0.08660887123298977 1107 28-29 -0.04458272319905632 1107 30-31 -0.015122859530997346 1107 32-33 0.013485170866594842 1107 34-35 -0.02682072378139111 1107 36-37 -0.009093607909058221 1107 38-39 -0.15775984137376753 1107 40-41 -0.17027597520599613 1107 42-43 -0.13676667146562949 1107 44-45 -0.04924033410476625 1107 46-47 -0.07149317788142184 1107 48-49 -0.1537435019161535 1107 50-51 -0.19818047592266907 1107 52-53 -0.06948852075557 1107 54-55 -0.1992417896273011 1107 56-57 -0.42557385413545745 1107 58-59 -0.4146854481512463 1107 60-61 -0.3111363839954393 1107 62-63 -0.1888256768006542 1107 64-65 -0.3455526990841875 1107 66-67 -0.2741711268036937 1107 68-69 -0.057064435757382626 1107 70-71 -0.2554392478214176 1107 72-73 -0.22075225840071866 1107 74-75 -0.16783180448842216 1107 76-77 -0.43659448647495225 1107 78-79 -0.3117238299516636 1107 80-81 -0.011342449676448041 1107 82-83 -0.045200144888863036 1107 84-85 0.060748214038628845 1107 86-87 0.03978103429103541 1107 88-89 -0.12970782128741476 1107 90-91 0.030183801297592794 1107 92-93 0.10832887549621617 1107 94-95 0.4431840187815652 1107 96-97 0.5951959808017868 1107 98-99 0.3410829682531791 1107 100-101 1.136625466501247 1108 1 0.12637833573763402 1108 2 0.13242954236075732 1108 3 0.022984977705675647 1108 4 0.15402912453934192 1108 5 0.2059012307862389 1108 6 0.14170517828698337 1108 7 0.14939576179357061 1108 8 0.12750676957112717 1108 9 0.21734244841807282 1108 10-11 0.15981101690703525 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2316 68-69 -1.6240240278576152 2316 70-71 -1.6040547140208083 2316 72-73 -1.1792653214697175 2316 74-75 -1.1905319336039781 2316 76-77 -1.2499088696846599 2316 78-79 -1.2992737304799817 2316 80-81 -0.9935230204199179 2316 82-83 -1.2651377939316966 2316 84-85 -1.0342236901523556 2316 86-87 -1.1936551193828286 2316 88-89 -1.3378027409854383 2316 90-91 -1.2567200273739694 2316 92-93 -1.5349080383784361 2316 94-95 -1.5656888880312536 2316 96-97 -1.564587981201349 2316 98-99 -2.170038756098279 2316 100-101 -3.2956616575155664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 12.0 4 26.0 5 55.0 6 151.0 7 349.0 8 874.0 9 1549.0 10 2659.0 11 4074.0 12 5865.0 13 8331.0 14 10152.0 15 13402.0 16 17085.0 17 21749.0 18 19010.0 19 12352.0 20 11335.0 21 11868.0 22 12505.0 23 14270.0 24 16463.0 25 19745.0 26 24561.0 27 29730.0 28 36868.0 29 45102.0 30 55865.0 31 68811.0 32 85705.0 33 107289.0 34 139649.0 35 187584.0 36 259400.0 37 331355.0 38 199553.0 39 11402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.895806265439546 19.337741819734966 14.867473912661364 21.898978002164128 2 15.783172117011649 21.493482031857415 38.92174911825933 23.801596732871616 3 20.241267055046325 25.314793503521837 30.00814970378961 24.435789737642228 4 12.785495973435701 17.13918888893242 37.35081228078325 32.72450285684863 5 13.191589629814535 38.25747929561722 33.650995495044214 14.899935579524026 6 30.860569332517702 36.62200666122292 17.34542823066793 15.171995775591448 7 27.9789831756829 33.529591847585486 20.640780317882825 17.85064465884879 8 26.83187827793228 33.446424503808295 19.58406254005157 20.13763467820786 9 26.475982491203347 16.867467856605174 20.705758303162316 35.95079134902917 10-11 25.110269487938773 26.71395526761024 26.801572008312256 21.37420323613873 12-13 26.871969320456163 24.21244308031217 27.483981893158894 21.431605706072776 14-15 23.01727686555532 25.382852195336405 27.152178518189558 24.447692420918724 16-17 21.59477019564474 28.641187759513176 27.536534026133353 22.227508018708736 18-19 21.432579136643337 27.103787885783877 30.367029703053415 21.096603274519367 20-21 22.640826211033495 25.58184959471311 30.26630899858347 21.511015195669923 22-23 21.998390380790237 25.783933806368854 30.40520876358186 21.81246704925905 24-25 22.214019909791975 25.867170670470944 30.576899290838888 21.34191012889819 26-27 21.992702866523125 25.782869142438503 30.91660794670037 21.307820044338005 28-29 21.328161051903688 26.777862135491425 30.641485842502686 21.252490970102205 30-31 21.670247219020386 25.929915200095373 30.477062777338897 21.922774803545348 32-33 21.6295559956801 26.128397520339437 30.534745724527745 21.707300759452718 34-35 22.464051607183634 25.956049269950434 30.237674676839454 21.34222444602648 36-37 22.352651790643936 25.64819667938159 30.50571555937877 21.4934359705957 38-39 21.853191764437895 26.206373734496196 30.528213689856216 21.412220811209696 40-41 21.98034370119471 26.25695009753713 30.217452807616933 21.54525339365123 42-43 21.802537585515758 26.446260870149256 29.525275948012464 22.225925596322522 44-45 22.14490198547622 26.06702206551092 29.477815556395083 22.310260392617778 46-47 22.669329967082792 26.6955943328564 28.885317549043503 21.749758151017303 48-49 22.614110148064963 26.82326201854261 28.59362770214851 21.969000131243916 50-51 21.95559383721285 27.118048821985536 28.93564400073541 21.990713340066204 52-53 22.61424542180202 27.40807155311874 27.999758221111833 21.977924803967415 54-55 22.04082272290847 26.848640152054692 28.360008294502986 22.750528830533845 56-57 22.610444554656077 26.940035668264983 28.071965706519364 22.377554070559576 58-59 23.227655154788803 26.637803697285356 27.996986994868877 22.137554153056964 60-61 22.49316204962734 26.93211983738945 28.87731099211019 21.69740712087302 62-63 22.341916927023643 26.89979426131528 28.737672401656994 22.02061641000409 64-65 22.04901622445258 27.42866483225981 28.417016600626336 22.10530234266127 66-67 22.34634573343348 27.09144649427494 27.89972161060633 22.662486161685248 68-69 22.218264050243477 27.051900962619342 28.246591067136535 22.483243920000646 70-71 22.5993301269647 27.105987861244664 28.00840517440401 22.28627683738663 72-73 22.532479907049556 26.732487910369535 28.427484744021086 22.307547438559826 74-75 22.1286525945633 27.83540178085454 27.74904273261744 22.28690289196472 76-77 22.371269556807512 27.122439019475937 27.78563824845331 22.720653175263248 78-79 22.384263573831806 27.223254920991547 27.92126413523932 22.471217369937328 80-81 22.721396386499514 27.296670276267402 27.642721737847825 22.33921159938526 82-83 22.435855093751538 27.114615567188977 28.05087146164628 22.3986578774132 84-85 22.207831106084953 27.300457393027695 27.92882695763656 22.562884543250796 86-87 22.317454711237524 27.367345185323664 27.54478080404083 22.77041929939798 88-89 22.135737885925035 27.373500910693778 27.30563980647267 23.18512139690851 90-91 22.260998645484943 26.983939001466506 27.623540004669973 23.131522348378585 92-93 22.505915486018797 27.129386018318762 27.739535545121868 22.625162950540574 94-95 22.15141010266591 26.96567315088832 27.833193929845894 23.04972281659988 96-97 21.76663349775928 26.90251309070567 28.22547092102235 23.105382490512703 98-99 21.925510706925902 27.233754815876715 27.41779410447093 23.42294037272646 100-101 21.51443775039677 26.912703719508574 27.755302503379692 23.81755602671496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 762.0 1 608.0 2 729.5 3 929.5 4 677.0 5 530.0 6 834.5 7 1624.0 8 2510.0 9 3035.0 10 2764.0 11 2313.0 12 2304.5 13 2452.0 14 2633.5 15 2774.0 16 2980.0 17 3251.0 18 3478.0 19 3700.5 20 4033.5 21 4565.0 22 5315.0 23 6163.5 24 7232.0 25 8732.5 26 10625.0 27 12943.5 28 15426.5 29 18520.0 30 21822.0 31 25378.0 32 29605.5 33 32523.5 34 35135.5 35 37865.5 36 40853.0 37 44536.5 38 46882.0 39 47836.0 40 48908.5 41 51512.0 42 54055.5 43 56099.5 44 58218.5 45 60480.5 46 62196.5 47 68937.5 48 86297.5 49 85990.5 50 68878.0 51 60347.5 52 56557.5 53 55633.0 54 54838.5 55 51914.0 56 47472.0 57 42768.5 58 39706.0 59 35765.5 60 30156.5 61 25513.0 62 21491.5 63 16818.5 64 12394.0 65 9347.0 66 6986.0 67 5393.5 68 3984.5 69 2601.0 70 1951.5 71 1595.5 72 1304.5 73 1127.5 74 894.5 75 691.5 76 504.0 77 324.0 78 198.5 79 130.5 80 89.0 81 62.0 82 47.5 83 29.5 84 18.0 85 10.5 86 5.5 87 4.0 88 2.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019924343462100935 2 0.013208272632179269 3 0.010689746070958647 4 0.0 5 0.0033580354149608314 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0017349849643964294 14-15 3.078199130380762E-4 16-17 0.0 18-19 0.0013152305375263257 20-21 0.0 22-23 5.596725691601386E-5 24-25 0.0 26-27 0.0016790177074804157 28-29 0.003106182758838769 30-31 0.0013152305375263257 32-33 0.008031301367447988 34-35 0.0017349849643964294 36-37 7.555579683661871E-4 38-39 0.001119345138320277 40-41 1.9588539920604848E-4 42-43 0.005596725691601386 44-45 0.001902886735144471 46-47 1.9588539920604848E-4 48-49 5.316889407021316E-4 50-51 1.3991814229003462E-4 52-53 1.3991814229003462E-4 54-55 0.0018749031066864644 56-57 0.016622275304056112 58-59 0.028123546600296964 60-61 0.022666739050985613 62-63 0.015083175738865733 64-65 0.019616523549062855 66-67 0.0029662646165487345 68-69 0.0010913615098622701 70-71 1.3991814229003462E-4 72-73 2.798362845800693E-5 74-75 0.0018189358497704502 76-77 0.002294657533556568 78-79 0.009962171731050467 80-81 0.01788153858466643 82-83 0.01874903106686464 84-85 0.030726024046891606 86-87 0.010941598727080708 88-89 0.0424231807423385 90-91 0.04925118608609219 92-93 0.031201745730677723 94-95 0.03229310724054 96-97 0.05316889407021316 98-99 0.05591128965909784 100-101 0.061172211809203146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1786759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.930123011220225 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3425409374776 45.38393033519633 2 13.118771186958977 15.199440572331701 3 3.8568034507258027 6.702752949919331 4 1.6448288092688654 3.81140541013377 5 0.8039054129953082 2.3285169732102 6 0.5001727183188036 1.7385040259438815 7 0.31918838937723826 1.294343586226367 8 0.2211110211634487 1.024719092410808 9 0.17067651679126405 0.8898580451560069 >10 0.9078289664806888 9.64513581110677 >50 0.07412491359505344 2.9469254114001546 >100 0.03510290790616144 3.792356984047757 >500 0.00271477510480069 1.0848070635430636 >1k 0.001745212567371872 1.957808833691652 >5k 3.878250149715271E-4 1.5913199608730493 >10k+ 9.695625374288178E-5 0.608174944809169 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10828 0.606013457886598 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9632 0.5390766186150454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8318 0.4655356430274033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5307 0.29701823245328557 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5075 0.2840338288487703 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3371 0.1886656230638827 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3369 0.1885536885500507 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2773 0.15519720342810642 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2551 0.14277247239275134 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2508 0.14036588034536276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2197 0.12296006344448243 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2146 0.12010573334176573 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2051 0.11478884393474442 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1928 0.10790487133407471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 5.5967256916013855E-5 0.0 0.0 0.0 0.0 18-19 1.1193451383202771E-4 0.0 0.0 5.5967256916013855E-5 0.0 20-21 1.1193451383202771E-4 0.0 0.0 1.1193451383202771E-4 0.0 22-23 1.6790177074804157E-4 0.0 0.0 2.2386902766405542E-4 0.0 24-25 1.6790177074804157E-4 0.0 0.0 5.037053122441247E-4 0.0 26-27 1.6790177074804157E-4 0.0 0.0 8.115252252822009E-4 0.0 28-29 2.798362845800693E-4 0.0 0.0 0.0012312796521523049 0.0 30-31 2.798362845800693E-4 0.0 0.0 0.0026864283319686655 0.0 32-33 2.798362845800693E-4 0.0 0.0 0.00464528232402915 0.0 34-35 3.358035414960832E-4 0.0 0.0 0.0061843818892195306 0.0 36-37 4.4773805532811084E-4 0.0 0.0 0.009038711991936238 0.0 38-39 6.156398260761524E-4 0.0 0.0 0.013460125288301332 0.0 40-41 6.156398260761524E-4 0.0 0.0 0.022191017367199493 0.0 42-43 6.436234545341593E-4 0.0 0.0 0.03058610590460157 0.0 44-45 7.275743399081801E-4 0.0 0.0 0.039540867011163786 0.0 46-47 7.275743399081801E-4 0.0 0.0 0.05865368524798252 0.0 48-49 7.275743399081801E-4 0.0 0.0 0.0714701870817497 0.0 50-51 7.275743399081801E-4 0.0 0.0 0.08775665884430972 0.0 52-53 7.275743399081801E-4 0.0 0.0 0.10384724520766371 0.0 54-55 7.555579683661871E-4 0.0 0.0 0.12184071830616217 0.0 56-57 7.83541596824194E-4 0.0 0.0 0.1732466437835209 0.0 58-59 7.83541596824194E-4 0.0 0.0 0.22641553785373406 0.0 60-61 7.83541596824194E-4 0.0 0.0 0.2649210106119516 0.0 62-63 7.83541596824194E-4 0.0 0.0 0.31154173562299114 0.0 64-65 7.83541596824194E-4 0.0 0.0 0.3617443650766555 0.0 66-67 7.83541596824194E-4 0.0 0.0 0.4430088221187077 0.0 68-69 7.83541596824194E-4 0.0 0.0 0.5431622283699145 0.0 70-71 8.115252252822009E-4 0.0 0.0 0.5949879082741433 0.0 72-73 8.954761106562217E-4 0.0 0.0 0.650451459877913 0.0 74-75 9.514433675722355E-4 0.0 0.0 0.7534591962318364 0.0 76-77 9.514433675722355E-4 0.0 0.0 0.8813443782849282 0.0 78-79 9.794269960302425E-4 0.0 0.0 1.0055916886384788 0.0 80-81 0.0012872469090683186 0.0 0.0 1.1019113377909386 0.0 82-83 0.0013991814229003465 0.0 0.0 1.2328747189744114 0.0 84-85 0.0014551486798163602 0.0 0.0 1.478626944092628 0.0 86-87 0.0015111159367323741 0.0 0.0 1.737671392728398 0.0 88-89 0.0015111159367323741 0.0 0.0 2.0405941707863233 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1590 0.0 28.382887 2 GTACCTG 1635 0.0 28.191475 1 GTACATG 9835 0.0 23.868021 1 TACATGG 9980 0.0 23.085606 2 TATCACG 105 0.0021956232 22.620947 2 GAGTACT 9935 0.0 22.275454 12-13 ACATGGG 10175 0.0 22.127144 3 AGTACTT 10115 0.0 21.010466 12-13 GTACTTT 10705 0.0 20.5839 14-15 CTGGTCG 295 3.0267984E-9 19.318249 9 CATGGGG 3760 0.0 19.198366 4 CATGGGA 6760 0.0 19.178934 4 TACTTTT 11275 0.0 18.700912 14-15 AGAGTAC 16630 0.0 18.605043 10-11 ACTTTTT 12250 0.0 18.201027 16-17 TCGCGCT 225 0.0 17.946201 60-61 TATAACG 190 1.8652098E-4 17.501469 2 ATAACGC 245 3.7650625E-6 17.44849 3 CTCGCGC 205 3.274181E-11 17.379751 60-61 GTATCAA 24235 0.0 17.313366 1 >>END_MODULE