FastQCFastQC Report
Thu 26 May 2016
SRR936483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615533
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77790.4815129124567557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62250.38532174830226307No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50140.3103619672269152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.22655061827892095No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32990.2042050518311913No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23900.14793879171765603No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22210.13747784786816486No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20200.12503613358563398No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19170.11866052875428729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.10529032833126899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16750.032.330452
GTACCTG17800.031.2219921
TATCACG1303.036439E-729.2326552
ATCACGC1303.0380943E-729.2308463
GTACATG100350.025.3712671
TATAACG1901.1910743E-825.0016142
TACATGG102500.024.238152
ACATGGG102000.022.9118253
TAACGCT1050.002195780622.6205064
GAGTACT72600.022.41609212-13
GTACACG2657.930794E-1021.5094911
TAACGCA2056.8285226E-720.8550034
GTACTTT80400.020.2099714-15
AGTACTT76400.020.11948812-13
CATGGGG32700.020.0471654
ACCTGGG26150.019.9809323
TCACGCA2151.0786807E-619.8850044
GTATCAA181750.019.810231
CATGGGA68750.019.7612764
ATAACGC2302.058856E-618.5870063
Produced by FastQC (version 0.11.5)