##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936483_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1615533 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.330536733078187 31.0 31.0 34.0 28.0 34.0 2 31.39267350156264 31.0 31.0 34.0 28.0 34.0 3 31.39238133792377 31.0 31.0 34.0 28.0 34.0 4 35.03182664792363 37.0 35.0 37.0 32.0 37.0 5 35.05468597670242 37.0 35.0 37.0 32.0 37.0 6 35.05228800649693 37.0 35.0 37.0 32.0 37.0 7 34.961693137806535 37.0 35.0 37.0 32.0 37.0 8 34.91872651316934 37.0 35.0 37.0 32.0 37.0 9 36.398736516060026 39.0 37.0 39.0 32.0 39.0 10-11 36.2553451399631 39.0 35.0 39.0 32.0 39.0 12-13 36.15819856357004 39.0 35.0 39.0 31.5 39.0 14-15 37.20498993211528 39.0 36.5 41.0 31.5 41.0 16-17 37.10281157983155 39.0 36.0 41.0 31.0 41.0 18-19 37.10772017656092 39.0 36.0 41.0 31.0 41.0 20-21 36.9875406444808 39.0 36.0 41.0 31.0 41.0 22-23 37.09531622071477 39.0 36.0 41.0 31.0 41.0 24-25 37.13837167052607 39.0 36.0 41.0 31.0 41.0 26-27 37.034168908960694 39.0 36.0 41.0 31.0 41.0 28-29 36.895272024774485 39.0 36.0 41.0 30.0 41.0 30-31 36.66918781603347 39.0 36.0 41.0 30.0 41.0 32-33 36.46419169401058 39.0 35.0 41.0 30.0 41.0 34-35 36.26269317927891 39.0 35.0 40.5 30.0 41.0 36-37 36.047121909611256 38.5 35.0 40.0 29.5 41.0 38-39 35.83322067701495 38.0 35.0 40.0 28.5 41.0 40-41 35.615397828456615 38.0 35.0 40.0 27.0 41.0 42-43 35.359265641741764 38.0 34.5 40.0 27.0 41.0 44-45 35.046985731643986 38.0 34.0 40.0 25.5 41.0 46-47 34.78054053987136 38.0 34.0 40.0 24.0 41.0 48-49 34.570170340067335 38.0 34.0 40.0 24.0 41.0 50-51 33.59344160719713 37.5 32.5 39.5 22.5 40.5 52-53 33.692533981045266 37.5 33.0 39.5 23.0 40.5 54-55 34.13105519973903 38.0 33.0 40.0 23.0 41.0 56-57 34.029896015742175 38.0 33.0 40.0 22.0 41.0 58-59 33.8027112414293 38.0 33.0 40.0 21.5 41.0 60-61 33.50015567617622 37.5 33.0 40.0 20.0 41.0 62-63 33.13871613888419 37.0 32.5 40.0 18.5 41.0 64-65 32.78579329546348 36.5 32.0 39.5 17.5 41.0 66-67 32.32628148109633 36.0 31.0 39.0 13.0 41.0 68-69 31.932481725845278 35.0 31.0 39.0 12.0 40.0 70-71 31.54242779318033 35.0 31.0 38.5 8.0 40.0 72-73 31.140077609061528 35.0 30.5 37.5 7.5 40.0 74-75 30.702855961469062 35.0 30.0 37.0 6.0 39.0 76-77 30.23580298266888 34.5 29.5 36.5 2.0 39.0 78-79 29.734225175220807 34.0 29.0 36.0 2.0 38.5 80-81 29.25868861855499 34.0 29.0 35.5 2.0 37.0 82-83 28.837414958406917 34.0 28.5 35.0 2.0 37.0 84-85 28.443029947391977 34.0 28.0 35.0 2.0 36.5 86-87 28.080674922765425 34.0 27.0 35.0 2.0 36.0 88-89 27.65648179269628 34.0 26.5 35.0 2.0 36.0 90-91 27.224881509693706 33.0 26.0 35.0 2.0 35.0 92-93 26.76596268847495 33.0 25.0 35.0 2.0 35.0 94-95 26.266805444395132 33.0 24.5 35.0 2.0 35.0 96-97 25.653029371730568 32.5 21.5 35.0 2.0 35.0 98-99 24.87563763785698 32.0 10.5 35.0 2.0 35.0 100-101 21.910778052816006 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7191455165400491 1101 2 0.5528455256489764 1101 3 0.5322008504908524 1101 4 0.6081307530192674 1101 5 0.5130561693434927 1101 6 0.5607683513742217 1101 7 0.5683832603738352 1101 8 0.5516619327505907 1101 9 0.6375721942904633 1101 10-11 0.8145427963165233 1101 12-13 0.8046817105081416 1101 14-15 0.94800431736261 1101 16-17 0.9958403534397959 1101 18-19 1.0433035304532723 1101 20-21 1.0316137510840733 1101 22-23 0.9376789909285179 1101 24-25 0.8908238168056144 1101 26-27 0.9468458779670144 1101 28-29 0.9271967182262344 1101 30-31 0.9753329754994198 1101 32-33 0.8523394293155562 1101 34-35 0.8124727459292771 1101 36-37 0.739919266404442 1101 38-39 0.6967638723284253 1101 40-41 0.7276001415140172 1101 42-43 0.7595771980680226 1101 44-45 0.6800306696296943 1101 46-47 0.948659848751447 1101 48-49 0.959498772429292 1101 50-51 0.8340925945329687 1101 52-53 0.9852785664037427 1101 54-55 0.857815554146427 1101 56-57 0.9184281723115006 1101 58-59 0.9489861232554944 1101 60-61 1.062169062064875 1101 62-63 1.0956465987976003 1101 64-65 0.7003995660851245 1101 66-67 0.924057601692347 1101 68-69 0.9816559354315473 1101 70-71 0.7780653836331517 1101 72-73 0.7826306216147323 1101 74-75 0.6398711980622842 1101 76-77 0.7394968153871027 1101 78-79 0.6195722097148995 1101 80-81 0.8701861008395078 1101 82-83 0.7186206777432069 1101 84-85 0.7007849700269411 1101 86-87 0.7388683473187321 1101 88-89 0.8431177821706761 1101 90-91 0.6802761365361611 1101 92-93 0.590517786587121 1101 94-95 0.5732872297350049 1101 96-97 0.6197705631976405 1101 98-99 0.733376473565361 1101 100-101 0.9221717337548974 1102 1 0.48234950888580386 1102 2 0.514655231702406 1102 3 0.4810257385854655 1102 4 0.4568190786112467 1102 5 0.42218393958129496 1102 6 0.525403299573199 1102 7 0.6229745422911037 1102 8 0.5842013209168613 1102 9 0.6891422415815498 1102 10-11 0.8029147521107376 1102 12-13 0.7186093998200178 1102 14-15 0.8512116394730853 1102 16-17 0.5923209185725185 1102 18-19 0.7201791574184639 1102 20-21 0.6892037179550172 1102 22-23 0.5802517188909491 1102 24-25 0.6405285636170461 1102 26-27 0.49582250217567037 1102 28-29 0.3393499379822771 1102 30-31 0.7058420501620191 1102 32-33 0.46931596079658533 1102 34-35 0.62749462121905 1102 36-37 0.5575979989020965 1102 38-39 0.5000504344095802 1102 40-41 0.5391537928750054 1102 42-43 0.424221840518733 1102 44-45 0.27067086958417264 1102 46-47 0.6539145546784297 1102 48-49 0.492663650576759 1102 50-51 0.6195779815022036 1102 52-53 0.6684704198603768 1102 54-55 0.6462952004326823 1102 56-57 0.695192526126398 1102 58-59 0.7890309697069711 1102 60-61 0.654116721535317 1102 62-63 0.7738564498433149 1102 64-65 0.8298770459168665 1102 66-67 0.8349579447845628 1102 68-69 0.7873723827833317 1102 70-71 0.6376675399719254 1102 72-73 0.4161626168603334 1102 74-75 0.2960287338677894 1102 76-77 0.2535665465612915 1102 78-79 0.26591916276943195 1102 80-81 0.42893474153681765 1102 82-83 0.4284816284542572 1102 84-85 0.31628776450037677 1102 86-87 0.42916271537996664 1102 88-89 0.2821483148487296 1102 90-91 0.28091300672591757 1102 92-93 0.42676564752940394 1102 94-95 0.44100115658133987 1102 96-97 0.2928782874088256 1102 98-99 0.43094068744584035 1102 100-101 0.6294190299016442 1103 1 0.29480399567203364 1103 2 0.3619507309733976 1103 3 0.3377019394570908 1103 4 0.5262608894196887 1103 5 0.5443055524819513 1103 6 0.4323969974371664 1103 7 0.62067995925986 1103 8 0.649762657192781 1103 9 0.44036402737266656 1103 10-11 0.5885781893642488 1103 12-13 0.3376747555998989 1103 14-15 0.6651872834302921 1103 16-17 0.8688466569422459 1103 18-19 0.8275204288405433 1103 20-21 0.8812599193046751 1103 22-23 0.6474875829050433 1103 24-25 0.7357884171780356 1103 26-27 0.6341653431562833 1103 28-29 0.8953979170760746 1103 30-31 0.748504762391029 1103 32-33 0.7195974057465193 1103 34-35 1.0164820169469877 1103 36-37 1.0591558056631527 1103 38-39 1.1506053570072297 1103 40-41 1.1168841873361401 1103 42-43 1.1979946767986434 1103 44-45 1.2471583649033846 1103 46-47 1.476926911964128 1103 48-49 1.31007599033439 1103 50-51 1.2196853408992396 1103 52-53 1.3040948173331586 1103 54-55 1.3286018140902343 1103 56-57 1.2214396081019743 1103 58-59 1.1988331415935463 1103 60-61 1.2643680500829575 1103 62-63 1.3004224230891168 1103 64-65 1.3163168967689671 1103 66-67 1.3242140377789084 1103 68-69 1.2056465854555611 1103 70-71 1.1506086297395193 1103 72-73 1.168503011879178 1103 74-75 1.0897861534179576 1103 76-77 1.2879558130117772 1103 78-79 1.1578044654870787 1103 80-81 1.254173362237463 1103 82-83 0.8431123838341819 1103 84-85 1.0214189062944534 1103 86-87 1.0892531044233316 1103 88-89 1.057858799021684 1103 90-91 1.0432234845250328 1103 92-93 0.8243535467908352 1103 94-95 0.9718579204225328 1103 96-97 1.1444524271393703 1103 98-99 1.0347791344592956 1103 100-101 1.2614332435687565 1104 1 0.4811442987604657 1104 2 0.5892701247965313 1104 3 0.44770846161323874 1104 4 0.6299643231430778 1104 5 0.5176471983672428 1104 6 0.6708876937732455 1104 7 0.6836793837754982 1104 8 0.6125188436497169 1104 9 0.8431869700160561 1104 10-11 0.8939956073577306 1104 12-13 0.8531948016386508 1104 14-15 0.6317483806871493 1104 16-17 0.706353850496825 1104 18-19 0.7585227307780116 1104 20-21 0.6966433837205628 1104 22-23 0.8136446901369538 1104 24-25 0.8519338228945159 1104 26-27 0.5934101158399656 1104 28-29 0.6569129755836585 1104 30-31 0.956380265939849 1104 32-33 0.7507374402755786 1104 34-35 0.8273864865422311 1104 36-37 0.6302831795361641 1104 38-39 0.7168221227850964 1104 40-41 0.5916551445584659 1104 42-43 0.8622636198256828 1104 44-45 0.8914386257896325 1104 46-47 0.7771920184287353 1104 48-49 1.3532121883010149 1104 50-51 1.0462498910622884 1104 52-53 0.91620204925335 1104 54-55 0.7825979775277929 1104 56-57 0.8572520001986561 1104 58-59 0.8167920903754435 1104 60-61 0.8770687982126262 1104 62-63 0.9675954520551642 1104 64-65 0.7758647578854081 1104 66-67 0.8690803335778767 1104 68-69 1.0248036926874846 1104 70-71 0.960372872977576 1104 72-73 0.6525214273788897 1104 74-75 0.7918979892130871 1104 76-77 0.8292686846787625 1104 78-79 0.9435608437721363 1104 80-81 0.958338526250504 1104 82-83 0.8607824394643337 1104 84-85 0.8780916475183176 1104 86-87 0.9037520493686593 1104 88-89 0.7855230998081844 1104 90-91 0.6987080423612078 1104 92-93 0.5083807863841585 1104 94-95 0.7177767771269252 1104 96-97 0.8359788035061442 1104 98-99 0.9286039953839094 1104 100-101 0.7947215612361234 1105 1 0.3417459878725424 1105 2 0.12088573767030297 1105 3 0.21854434383190835 1105 4 0.2560606481830163 1105 5 0.25319615979157106 1105 6 0.43449782844569995 1105 7 0.3691411504954232 1105 8 0.4518760680277154 1105 9 0.5886350412326706 1105 10-11 0.5231003779580519 1105 12-13 0.3921420739509074 1105 14-15 0.4988344340996136 1105 16-17 0.4030669297024829 1105 18-19 0.4276667618211576 1105 20-21 0.32640188634552914 1105 22-23 0.22952599577493515 1105 24-25 0.4156425651907938 1105 26-27 0.2252941605786063 1105 28-29 0.16283947638255825 1105 30-31 0.1261679856385527 1105 32-33 -0.13150314406589558 1105 34-35 0.07595074127859647 1105 36-37 0.2520946381072662 1105 38-39 0.17364567905078587 1105 40-41 0.30391901125556586 1105 42-43 0.11094930802472902 1105 44-45 0.28580155662734086 1105 46-47 0.23798087941938206 1105 48-49 0.26731216547517533 1105 50-51 0.29535466166261415 1105 52-53 0.17982222729489905 1105 54-55 -0.03620686155003483 1105 56-57 -0.09312006097459147 1105 58-59 -0.09837339695621239 1105 60-61 0.02301538574972284 1105 62-63 0.06267054906497549 1105 64-65 4.764903665801512E-4 1105 66-67 0.06842842762033285 1105 68-69 0.20407965707917697 1105 70-71 -0.014384488355450031 1105 72-73 -0.10955481092286945 1105 74-75 0.07856587325782627 1105 76-77 0.140529127500038 1105 78-79 0.42338828043043364 1105 80-81 0.41413491976796735 1105 82-83 0.18811227283570986 1105 84-85 0.10730415607800481 1105 86-87 -0.091955779894068 1105 88-89 -0.07454720377547375 1105 90-91 0.006036962699031534 1105 92-93 -0.012552333908011803 1105 94-95 0.24309063241110707 1105 96-97 0.17215227988140214 1105 98-99 0.45462362560531844 1105 100-101 0.6256998766892146 1106 1 0.46406292926291215 1106 2 0.3251669678378839 1106 3 0.4599930325336459 1106 4 0.4719883338144868 1106 5 0.24955471281811015 1106 6 0.5712896501983238 1106 7 0.6861391436687825 1106 8 0.6074926097679949 1106 9 0.7123430393802153 1106 10-11 0.6407861809459874 1106 12-13 0.7245651884772499 1106 14-15 0.6932050280859912 1106 16-17 0.8085548287093616 1106 18-19 0.6114929397597777 1106 20-21 0.3928141262728104 1106 22-23 0.6119568288652815 1106 24-25 0.5397870910136788 1106 26-27 0.5047644955642809 1106 28-29 0.48370532774016084 1106 30-31 0.5934678604262942 1106 32-33 0.4589704327166615 1106 34-35 0.4533713687120766 1106 36-37 0.4608852248896511 1106 38-39 0.6619426207842096 1106 40-41 0.5931944953810557 1106 42-43 0.5130942111996291 1106 44-45 0.4452696618056322 1106 46-47 0.5587678299005034 1106 48-49 0.4916239268514815 1106 50-51 0.3485895975985329 1106 52-53 0.40296505503369673 1106 54-55 0.6915859721920938 1106 56-57 0.5180701415948334 1106 58-59 0.5506249049596974 1106 60-61 0.7206294918542469 1106 62-63 0.6532761990538276 1106 64-65 0.652786056240231 1106 66-67 0.8328535661256709 1106 68-69 0.6676591840169621 1106 70-71 0.3796267635956312 1106 72-73 0.32062992004229685 1106 74-75 0.5736534360783558 1106 76-77 0.6592277776089546 1106 78-79 0.44718289801846467 1106 80-81 0.7704221189583755 1106 82-83 0.45570373781915663 1106 84-85 0.5922623900705624 1106 86-87 0.6542038057048103 1106 88-89 0.6149263901594502 1106 90-91 0.45014512550927677 1106 92-93 0.3791313421867386 1106 94-95 0.5589728642767717 1106 96-97 0.6125924095532795 1106 98-99 0.5320579749303747 1106 100-101 0.4750523749310993 1107 1 0.20219168690240963 1107 2 0.15462520524616608 1107 3 0.25270383300402344 1107 4 0.25285522021162166 1107 5 0.1284208373357245 1107 6 0.24989408566216298 1107 7 0.25101815388790527 1107 8 0.37505972969776735 1107 9 0.4621898353455691 1107 10-11 0.49175095229932 1107 12-13 0.4223938318348175 1107 14-15 0.06130711994216398 1107 16-17 0.08883125847565765 1107 18-19 0.04764329502751252 1107 20-21 0.18248072116821845 1107 22-23 0.2956045755237753 1107 24-25 0.19216216761569882 1107 26-27 0.250114546080205 1107 28-29 0.16320083099471816 1107 30-31 0.23413649252129431 1107 32-33 0.15122410547284204 1107 34-35 0.3036968237694708 1107 36-37 0.24346634021502211 1107 38-39 0.006217758667837359 1107 40-41 0.1889784859735002 1107 42-43 0.42132996322440874 1107 44-45 0.442156721447553 1107 46-47 0.3120679276198359 1107 48-49 0.32141672874624305 1107 50-51 0.38119721308125065 1107 52-53 0.39637396902412547 1107 54-55 0.3643330447528328 1107 56-57 0.054241200110489274 1107 58-59 0.2611429830357892 1107 60-61 0.4529083397599081 1107 62-63 0.333155954589877 1107 64-65 0.27898311803528486 1107 66-67 0.35721963267419454 1107 68-69 0.3973882199081089 1107 70-71 0.14923688003069557 1107 72-73 0.1429529900855222 1107 74-75 0.29996146728229434 1107 76-77 0.3140524625055221 1107 78-79 0.11606426012655291 1107 80-81 0.029991160149556606 1107 82-83 0.1935053841414316 1107 84-85 0.0012699591780069852 1107 86-87 -0.1366693744796983 1107 88-89 -0.2706012114106855 1107 90-91 -0.004330088562308987 1107 92-93 0.1645156772812264 1107 94-95 0.11680697329179424 1107 96-97 0.016439901147759173 1107 98-99 0.06022929844760583 1107 100-101 -0.10132604731578354 1108 1 0.04436822648610672 1108 2 0.0042133753885842395 1108 3 0.04189072632685864 1108 4 0.013140184909588015 1108 5 0.046016639644321344 1108 6 -0.014877063704169302 1108 7 -0.0252963201101295 1108 8 -0.057930766033642556 1108 9 -0.12256445529751403 1108 10-11 0.0025841522222194158 1108 12-13 -0.1964510189034101 1108 14-15 0.01130140235191135 1108 16-17 0.14893783435788777 1108 18-19 0.17069008451890966 1108 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2316 82-83 -2.6714485813134488 2316 84-85 -2.621749374768747 2316 86-87 -2.527164688569659 2316 88-89 -2.8172567965544495 2316 90-91 -3.0190543966386407 2316 92-93 -3.613244006089552 2316 94-95 -3.6207753182596996 2316 96-97 -3.7408843046087625 2316 98-99 -5.138923096229515 2316 100-101 -5.645446874644541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3736.0 3 1492.0 4 1487.0 5 1677.0 6 1853.0 7 2296.0 8 2853.0 9 3736.0 10 4722.0 11 6144.0 12 8038.0 13 9684.0 14 10796.0 15 12989.0 16 15851.0 17 19729.0 18 15702.0 19 11905.0 20 11628.0 21 12803.0 22 13664.0 23 15449.0 24 17612.0 25 20138.0 26 23582.0 27 27474.0 28 32411.0 29 38670.0 30 46861.0 31 56482.0 32 69926.0 33 88142.0 34 115280.0 35 154955.0 36 211613.0 37 275584.0 38 217383.0 39 31179.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.3230319688978 18.811133397716862 14.283237996186514 21.582596637198822 2 15.256796460900151 20.719718496452106 40.49783139750118 23.52565364514655 3 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22.06632025376514 27.314571656805487 28.817441184963627 21.801666904465744 48-49 22.047676171941735 27.438311037161938 28.623746846224506 21.89026594467182 50-51 21.800044569348668 27.72665370854122 28.708726926076782 21.76457479603333 52-53 22.08413069967988 27.957389609059135 28.22498900477847 21.73349068648252 54-55 21.6592135335032 27.57844979782448 28.537112146130934 22.225224522541396 56-57 22.085915963307183 27.9262318243563 27.94257330686921 22.04527890546731 58-59 22.354285933600096 27.438833396061963 28.069039459609368 22.137841210728578 60-61 21.999835962320855 27.637872895559067 28.725473658798535 21.636817483321547 62-63 21.995766069129445 27.63670520946816 28.64059250272358 21.726936218678816 64-65 21.75745814802091 27.957214881941674 28.416325444208194 21.869001525829223 66-67 21.82667876812743 27.87906009786829 28.053586110230604 22.240675023773672 68-69 21.79583841782964 27.876612163343385 28.301557876249888 22.02599154257709 70-71 21.96002083435265 27.80850356209213 28.089680616310375 22.141794987244843 72-73 21.960429611455893 27.62634315488987 28.262241657527348 22.150985576126892 74-75 21.61040837916132 28.42517971289899 27.985552999322294 21.978858908617397 76-77 21.903524164158018 27.849428943989086 28.09762129135559 22.149425600497313 78-79 21.899688838570146 27.873133588835845 28.081823564532556 22.14535400806146 80-81 21.927728010506883 28.156494319097003 27.833915672353765 22.08186199804235 82-83 21.893418566441383 28.08413156797912 28.059885713153264 21.962564152426225 84-85 21.808538300759775 28.10983175005696 27.840610791374033 22.241019157809234 86-87 21.843932112093984 28.10392143205405 27.918398303576264 22.1337481522757 88-89 21.94687268402286 27.999819210606642 27.566605722479615 22.486702382890883 90-91 21.820623920567385 27.86405326057542 27.948673965989475 22.36664885286772 92-93 22.036882877098456 28.038701356677404 27.948458456224145 21.97595731 94-95 21.66326481970211 27.889263845269515 27.974573128566288 22.472898206462087 96-97 21.482390437852356 27.77216100524888 28.124748483471418 22.62070007342735 98-99 21.57322249323945 28.00841767054345 27.600756426044864 22.81760341017224 100-101 21.67380246234505 27.821055057123534 27.740343757045977 22.76479872348544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 343.0 1 267.5 2 455.0 3 661.5 4 520.0 5 485.0 6 725.5 7 1298.5 8 2042.0 9 2477.5 10 2278.0 11 1961.0 12 2019.5 13 2124.0 14 2164.0 15 2250.5 16 2421.0 17 2677.0 18 2877.5 19 3042.5 20 3303.0 21 3764.5 22 4418.5 23 5171.5 24 6152.0 25 7560.0 26 9562.5 27 12021.0 28 14338.0 29 16984.5 30 20243.0 31 24032.5 32 28822.5 33 32990.0 34 36626.0 35 40011.5 36 44401.5 37 48511.0 38 49702.0 39 50395.5 40 50985.5 41 52175.0 42 52857.5 43 53923.0 44 56148.0 45 57942.5 46 58497.5 47 61682.0 48 73582.0 49 72831.5 50 59809.0 51 52755.5 52 48307.5 53 46276.5 54 44348.5 55 40916.0 56 36937.0 57 33362.5 58 30869.5 59 27796.5 60 23602.0 61 20006.0 62 16778.5 63 12838.5 64 9159.5 65 6888.5 66 5161.5 67 3965.5 68 3046.0 69 2209.5 70 1694.5 71 1339.0 72 1121.5 73 1023.5 74 820.0 75 622.5 76 466.5 77 295.0 78 175.0 79 119.5 80 76.0 81 54.5 82 36.0 83 21.0 84 15.5 85 13.5 86 12.0 87 7.5 88 7.5 89 7.5 90 3.5 91 1.0 92 1.0 93 1.5 94 2.5 95 3.5 96 1.5 97 0.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01318450319492081 2 0.012813108738725857 3 0.015041475475895572 4 0.019436309874202508 5 0.030206749103856127 6 0.049209765445831194 7 0.006499402983411667 8 0.025564318401419223 9 0.03497297795835801 10-11 0.0401415508070711 12-13 0.03150662970053846 14-15 0.027390341144377738 16-17 0.029866304185677418 18-19 0.024852479027045564 20-21 0.056297209651551536 22-23 0.01318450319492081 24-25 0.0026926098074134047 26-27 0.0014236787487473175 28-29 3.0949538016246034E-5 30-31 0.0030021051875758647 32-33 0.002537862117332175 34-35 0.0 36-37 4.332935322274444E-4 38-39 6.189907603249207E-5 40-41 0.0016712750528772857 42-43 0.0045495820883881665 44-45 0.0023521648892346984 46-47 0.0014546282867635635 48-49 5.570916842924286E-4 50-51 0.004518632550371921 52-53 0.0036210959479007856 54-55 0.006499402983411667 56-57 7.737384504061509E-4 58-59 0.0067469992875416355 60-61 0.003125903339640849 62-63 8.046879884223969E-4 64-65 0.0011141833685848572 66-67 0.01863162188578011 68-69 0.045712467649995384 70-71 0.05521397582098292 72-73 0.01606281023043169 74-75 0.03258986353110707 76-77 0.028164079594783887 78-79 0.057999434242445067 80-81 0.08374944987196176 82-83 0.05100483865077346 84-85 0.019622007102299985 86-87 0.022933607670038308 88-89 0.02438823595680187 90-91 0.0420913717020946 92-93 0.027699836524540198 94-95 0.014484383791603142 96-97 0.020488594166754873 98-99 0.05165477894911463 100-101 0.07037924944894347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1615533.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.47641273533 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11343145222698 49.05449358883776 2 11.662947183700364 14.106664151836357 3 3.254786948411883 5.905135165731668 4 1.299872092384904 3.1444640464882587 5 0.6837874084095341 2.067650476709832 6 0.3920401368795341 1.4225508676045182 7 0.2680791563802708 1.1348725994894628 8 0.1900519159550906 0.9194926488352246 9 0.1377031347929715 0.7495012453219674 >10 0.8739291371741444 10.15223266535569 >50 0.07992951623179924 3.3227266607586228 >100 0.03932440165371431 4.298909561240228 >500 0.002470509479287388 0.9966106178888715 >1k 0.0013381926346140017 1.5407653690267704 >5k 3.088136849109235E-4 1.1839303348747927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7779 0.4815129124567557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6225 0.38532174830226307 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5014 0.3103619672269152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3660 0.22655061827892095 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3299 0.2042050518311913 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2390 0.14793879171765603 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2221 0.13747784786816486 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2020 0.12503613358563398 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1917 0.11866052875428729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1701 0.10529032833126899 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.189907603249207E-5 7 6.189907603249207E-5 0.0 0.0 0.0 6.189907603249207E-5 8 6.189907603249207E-5 0.0 0.0 0.0 6.189907603249207E-5 9 6.189907603249207E-5 0.0 0.0 0.0 6.189907603249207E-5 10-11 6.189907603249207E-5 0.0 0.0 0.0 6.189907603249207E-5 12-13 6.189907603249207E-5 0.0 0.0 0.0 1.2379815206498414E-4 14-15 1.2379815206498414E-4 0.0 0.0 0.0 1.2379815206498414E-4 16-17 1.2379815206498414E-4 0.0 0.0 0.0 1.2379815206498414E-4 18-19 1.2379815206498414E-4 0.0 0.0 0.0 1.2379815206498414E-4 20-21 1.2379815206498414E-4 0.0 0.0 0.0 1.2379815206498414E-4 22-23 1.2379815206498414E-4 0.0 0.0 1.5474769008123017E-4 1.2379815206498414E-4 24-25 1.8569722809747618E-4 0.0 0.0 3.713944561949524E-4 1.2379815206498414E-4 26-27 2.4759630412996827E-4 0.0 0.0 6.808898363574126E-4 1.2379815206498414E-4 28-29 2.4759630412996827E-4 0.0 0.0 0.0012998805966823333 1.2379815206498414E-4 30-31 2.4759630412996827E-4 0.0 0.0 0.0025997611933646666 1.2379815206498414E-4 32-33 2.4759630412996827E-4 0.0 0.0 0.004735279316485643 2.4759630412996827E-4 34-35 2.785458421462143E-4 0.0 0.0 0.006870797439606619 2.4759630412996827E-4 36-37 3.0949538016246034E-4 0.0 0.0 0.009934801703214976 2.4759630412996827E-4 38-39 3.7139445619495235E-4 0.0 0.0 0.013927292107310713 2.4759630412996827E-4 40-41 3.7139445619495235E-4 0.0 0.0 0.018910167727926325 2.4759630412996827E-4 42-43 4.6424307024369046E-4 0.0 0.0 0.024140639652671905 2.785458421462143E-4 44-45 4.951926082599365E-4 0.0 0.0 0.027049896226199032 3.0949538016246034E-4 46-47 4.951926082599365E-4 0.0 0.0 0.030609093098067322 3.0949538016246034E-4 48-49 4.951926082599365E-4 0.0 0.0 0.0362419090170241 3.0949538016246034E-4 50-51 4.951926082599365E-4 0.0 0.0 0.04264846338638703 3.0949538016246034E-4 52-53 4.951926082599365E-4 0.0 0.0 0.05403789337636557 3.0949538016246034E-4 54-55 4.951926082599365E-4 0.0 0.0 0.061651479728362095 3.0949538016246034E-4 56-57 4.951926082599365E-4 0.0 0.0 0.0830376104975881 3.0949538016246034E-4 58-59 4.951926082599365E-4 0.0 0.0 0.11785584076586489 3.0949538016246034E-4 60-61 5.261421462761826E-4 0.0 0.0 0.14561757636643757 3.0949538016246034E-4 62-63 5.570916842924286E-4 0.0 0.0 0.16282551950347035 3.0949538016246034E-4 64-65 5.570916842924286E-4 0.0 0.0 0.19609627287093484 3.0949538016246034E-4 66-67 5.570916842924286E-4 0.0 0.0 0.26594938017360215 3.404449181787063E-4 68-69 5.570916842924286E-4 0.0 0.0 0.3582409025380478 3.7139445619495235E-4 70-71 5.570916842924286E-4 0.0 0.0 0.43843115553814127 3.7139445619495235E-4 72-73 5.570916842924286E-4 0.0 0.0 0.5328581960257079 4.332935322274445E-4 74-75 5.570916842924286E-4 0.0 0.0 0.6444622301122911 4.951926082599365E-4 76-77 5.570916842924286E-4 0.0 0.0 0.7179983324388917 4.951926082599365E-4 78-79 5.570916842924286E-4 0.0 0.0 0.8012525897025935 4.951926082599365E-4 80-81 5.570916842924286E-4 0.0 0.0 0.9247721959254314 4.951926082599365E-4 82-83 5.570916842924286E-4 0.0 0.0 1.0380784546029083 4.951926082599365E-4 84-85 5.570916842924286E-4 0.0 0.0 1.1865743380048568 4.951926082599365E-4 86-87 6.189907603249207E-4 0.0 0.0 1.4163437082374672 4.951926082599365E-4 88-89 6.189907603249207E-4 0.0 0.0 1.6964989263605261 4.951926082599365E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1675 0.0 32.33045 2 GTACCTG 1780 0.0 31.221992 1 TATCACG 130 3.036439E-7 29.232655 2 ATCACGC 130 3.0380943E-7 29.230846 3 GTACATG 10035 0.0 25.371267 1 TATAACG 190 1.1910743E-8 25.001614 2 TACATGG 10250 0.0 24.23815 2 ACATGGG 10200 0.0 22.911825 3 TAACGCT 105 0.0021957806 22.620506 4 GAGTACT 7260 0.0 22.416092 12-13 GTACACG 265 7.930794E-10 21.509491 1 TAACGCA 205 6.8285226E-7 20.855003 4 GTACTTT 8040 0.0 20.20997 14-15 AGTACTT 7640 0.0 20.119488 12-13 CATGGGG 3270 0.0 20.047165 4 ACCTGGG 2615 0.0 19.980932 3 TCACGCA 215 1.0786807E-6 19.885004 4 GTATCAA 18175 0.0 19.81023 1 CATGGGA 6875 0.0 19.761276 4 ATAACGC 230 2.058856E-6 18.587006 3 >>END_MODULE