FastQCFastQC Report
Thu 26 May 2016
SRR936483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615533
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94310.5837701860624327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83640.5177238719357635No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59180.366318731960288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50210.31079526075914266No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27930.17288411935875034No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25060.15511908453742512No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.14533903052429137No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23270.14403914992760905No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18980.11748444630966995No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18940.11723685000553996No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.10052409947676712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1152.4992914E-937.180132
TACCTGG16350.032.5436672
GTACCTG16900.032.330891
CGTATAC700.0083895227.1448733
GTACATG98100.023.6835291
TACATGG101250.022.6630462
GAGTACT89500.022.15775312-13
ACATGGG101450.021.3051823
AGTACTT93000.020.5832712-13
GTACTTT97200.020.13399514-15
GTGTAGC8400.019.7968161
GGTTACG600.00476669919.79068628-29
GCACCGT3900.019.4856116
ATCACGC2201.3444824E-619.4332623
GTATCAA196150.019.3537451
ACCTGGG25800.019.148713
TAGGTCG1507.720659E-419.0008245
CATGGGG32050.018.971184
AGCACCG3305.984475E-1018.7129335
CATGGGA65000.018.7085044
Produced by FastQC (version 0.11.5)