FastQCFastQC Report
Thu 26 May 2016
SRR936482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607752
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78540.48850817787818024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.39720056327095227No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49710.3091894769840125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39310.24450288352930055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32930.20482014639073687No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23810.14809497982275874No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22050.1371480178534998No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20250.12595226129403042No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19260.11979459518632228No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.10368514547019689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG801.0752045E-535.6378942
TACCTGG14850.033.5980152
GTACCTG18000.029.0391881
ATCCCGT1201.707396E-423.7578566
GTACATG99850.023.6998481
GAGTACT72600.023.65747512-13
TACATGG102200.022.7822172
GTATCAA173600.022.609691
ACATGGG101650.021.9231383
AGTACTT77200.021.20160112-13
GTACTTT81750.020.9504814-15
ATCAACG198850.019.6657943
TCAACGC199250.019.6269264
CAACGCA199150.019.565815
TATCAAC198450.019.5384372
AACGCAG202050.019.2837856
TACACCG1507.7024987E-419.007475
TAACGCA2251.6627073E-619.0068764
CATGGGA65300.018.9923234
GGTATCA133000.018.8288291
Produced by FastQC (version 0.11.5)