FastQCFastQC Report
Thu 26 May 2016
SRR936482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607752
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95450.5936860908896396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82400.5125168558334867No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57080.35502988023028426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.3144141633784315No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27650.17197926048296006No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24360.15151590543815216No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22880.14231050560036623No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22770.14162632047728754No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18260.1135747304310615No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17990.1118953669471411No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.10878543456951072No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16630.10343635087998646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1551.3587851E-930.6435592
GTACCTG17300.030.2346461
TACCTGG17900.028.1270182
TACCGTA700.00840106227.1372177
GTACCGT1109.492341E-525.9037046
TAACGCA1901.19289325E-824.9979114
CCGTATG1351.3453271E-524.6245129
GTACATG96600.023.0000271
GAGTACT91100.022.4150612-13
TATCACG1302.943257E-421.9219282
TACATGG99300.021.9072112
ACATGGG97150.021.6579953
GTACTTT97850.020.72320214-15
AGTACTT95000.019.69529212-13
GGTATCA155600.019.0899811
CATGGGA63300.018.8333424
GTGTAGC8450.018.7405241
ACTTTTT112250.018.36086816-17
CATGGGG33200.018.3117244
ACCTGGG26200.018.3095423
Produced by FastQC (version 0.11.5)