##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936482_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1607752 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.502181928556144 31.0 31.0 34.0 30.0 34.0 2 31.70711527648543 31.0 31.0 34.0 30.0 34.0 3 31.981755270713393 31.0 31.0 34.0 30.0 34.0 4 35.78921997920077 37.0 35.0 37.0 35.0 37.0 5 35.613569754539256 37.0 35.0 37.0 33.0 37.0 6 35.720371674238315 37.0 35.0 37.0 33.0 37.0 7 35.657390567699494 37.0 35.0 37.0 33.0 37.0 8 35.64060128676562 37.0 35.0 37.0 33.0 37.0 9 37.26669411700312 39.0 37.0 39.0 34.0 39.0 10-11 37.16270917405172 39.0 37.0 39.0 33.0 39.0 12-13 37.03840175599222 39.0 37.0 39.0 33.0 39.0 14-15 38.13261093750778 40.0 37.0 41.0 33.0 41.0 16-17 38.05824996641273 40.0 37.0 41.0 33.0 41.0 18-19 38.168090601037974 40.0 37.5 41.0 33.5 41.0 20-21 38.2533584159746 40.0 38.0 41.0 33.5 41.0 22-23 38.16723723559355 40.0 37.0 41.0 33.0 41.0 24-25 38.065079377914 40.0 37.0 41.0 33.0 41.0 26-27 37.85730541775099 40.0 37.0 41.0 33.0 41.0 28-29 37.769651662694244 40.0 37.0 41.0 33.0 41.0 30-31 37.714189750658065 40.0 37.0 41.0 33.0 41.0 32-33 37.50441392702357 39.5 36.0 41.0 32.5 41.0 34-35 37.31771069169872 39.0 36.0 41.0 32.0 41.0 36-37 37.0644517935602 39.0 36.0 41.0 31.0 41.0 38-39 36.909608882464454 39.0 35.0 41.0 31.0 41.0 40-41 36.673080176544644 39.0 35.0 41.0 30.5 41.0 42-43 36.416883636282215 39.0 35.0 40.5 30.0 41.0 44-45 36.103752320009555 39.0 35.0 40.0 30.0 41.0 46-47 35.654288721146045 39.0 35.0 40.0 28.5 41.0 48-49 35.16393635336793 38.0 35.0 40.0 26.0 41.0 50-51 34.95129566002717 38.0 34.0 40.0 26.0 41.0 52-53 34.6830611935174 38.0 34.0 40.0 24.5 41.0 54-55 34.4250776861108 38.0 34.0 40.0 24.0 41.0 56-57 34.15456146221557 38.0 33.5 40.0 23.5 41.0 58-59 33.80619492309759 37.5 33.0 40.0 22.5 41.0 60-61 33.44196897282666 37.0 33.0 40.0 22.0 41.0 62-63 33.16092531683991 36.5 32.0 39.0 22.0 41.0 64-65 32.7314843956033 36.0 32.0 39.0 20.0 40.0 66-67 32.32888125780593 35.5 31.0 39.0 19.0 40.0 68-69 31.888929076126168 35.0 31.0 38.0 18.0 40.0 70-71 31.415765615592456 35.0 30.5 37.5 15.5 40.0 72-73 31.001653240051947 35.0 30.0 37.0 13.0 39.0 74-75 30.649506267135727 34.0 30.0 36.5 9.5 39.0 76-77 29.208253200742405 32.5 28.5 35.0 8.0 37.5 78-79 29.997630231528248 34.0 29.5 36.0 7.0 38.0 80-81 29.832105946688294 34.0 29.5 35.0 6.0 37.0 82-83 29.487335733371815 34.0 29.0 35.0 2.0 37.0 84-85 29.031456655006494 34.0 29.0 35.0 2.0 36.0 86-87 28.65326493140733 34.0 29.0 35.0 2.0 36.0 88-89 28.22121913081122 33.0 28.0 35.0 2.0 35.5 90-91 27.76242075892302 33.0 27.0 35.0 2.0 35.0 92-93 27.320213254282997 33.0 26.0 35.0 2.0 35.0 94-95 26.799537179863563 33.0 25.5 34.5 2.0 35.0 96-97 26.13571465002065 32.0 24.5 34.0 2.0 35.0 98-99 25.29524422920948 32.0 21.5 34.0 2.0 35.0 100-101 22.48595430140967 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34745732442139854 1101 2 0.3917151867612674 1101 3 0.21304702046703383 1101 4 0.20628907561093968 1101 5 0.27139064481666964 1101 6 0.26345964275742517 1101 7 0.2563595786248527 1101 8 0.2103602802555642 1101 9 0.31311074238875847 1101 10-11 0.29957782262325594 1101 12-13 0.38297374002997486 1101 14-15 0.4551351199921001 1101 16-17 0.3864015787690107 1101 18-19 0.34276536169997485 1101 20-21 0.31807336742650705 1101 22-23 0.25228419340087527 1101 24-25 0.23503570927854867 1101 26-27 0.3642267216684445 1101 28-29 0.3706149455497254 1101 30-31 0.3333366185901312 1101 32-33 0.4057829119789318 1101 34-35 0.49686977554613776 1101 36-37 0.4868847422946061 1101 38-39 0.5120876760370265 1101 40-41 0.6776706452402692 1101 42-43 0.7805500433839256 1101 44-45 0.7913517786587789 1101 46-47 0.772052544596697 1101 48-49 0.7755288360028274 1101 50-51 0.7335977391092925 1101 52-53 0.6334053883847091 1101 54-55 0.5375516070322703 1101 56-57 0.5707106814203016 1101 58-59 0.5623306073554701 1101 60-61 0.4657306632365561 1101 62-63 0.383029512097373 1101 64-65 0.5799653373279412 1101 66-67 0.4532179155394189 1101 68-69 0.3301895751788102 1101 70-71 0.26847571787879687 1101 72-73 0.22300569883695687 1101 74-75 0.1725231666930256 1101 76-77 0.6105791591960603 1101 78-79 0.5254097338436701 1101 80-81 0.38099037512777656 1101 82-83 0.5381469996398529 1101 84-85 0.5748928942062612 1101 86-87 0.39148492104402166 1101 88-89 0.17567028388285877 1101 90-91 0.27951157962554163 1101 92-93 0.5789646510859932 1101 94-95 0.5035663819791658 1101 96-97 0.5309420916501786 1101 98-99 0.46878829786388465 1101 100-101 0.47542146508646965 1102 1 0.5407939256604202 1102 2 0.5729109968889929 1102 3 0.27360322496840084 1102 4 0.1833546353614679 1102 5 0.18387682574739728 1102 6 0.1921723660353436 1102 7 0.2895366434893063 1102 8 0.06576153735630896 1102 9 0.16056560009580068 1102 10-11 0.22567057204441454 1102 12-13 0.3181623051412643 1102 14-15 0.2734669410499251 1102 16-17 0.3541236225311408 1102 18-19 0.32933218341833737 1102 20-21 0.32380741205079744 1102 22-23 0.17286235197078526 1102 24-25 0.27574242864888276 1102 26-27 0.0601846004500004 1102 28-29 0.0751888836541994 1102 30-31 0.07058135691431744 1102 32-33 0.28013628383183686 1102 34-35 0.31902096137873315 1102 36-37 0.3209833391810619 1102 38-39 0.28103631429377174 1102 40-41 0.387153394870019 1102 42-43 0.18287008259879656 1102 44-45 0.14069046231855964 1102 46-47 0.043826704501583436 1102 48-49 -0.2801563638608897 1102 50-51 -0.2798276170576628 1102 52-53 -0.3423903814156759 1102 54-55 -0.07356631221568932 1102 56-57 -0.1104525365629101 1102 58-59 -0.03437897664708345 1102 60-61 -0.03531011257815919 1102 62-63 -0.12047262247081392 1102 64-65 -0.14037494204323053 1102 66-67 -0.048485567213688796 1102 68-69 0.007629466573092714 1102 70-71 -0.2619271036448829 1102 72-73 -0.045540969637489326 1102 74-75 -0.35849716732865744 1102 76-77 -0.22856296341297977 1102 78-79 -0.044540395829610446 1102 80-81 -0.07355618557530974 1102 82-83 -0.03757628523112899 1102 84-85 0.027921530098989678 1102 86-87 -0.3317860439615785 1102 88-89 -0.32591717083578686 1102 90-91 -0.18483826358451694 1102 92-93 0.20558730532071223 1102 94-95 0.04250791080279015 1102 96-97 -0.039802417225313746 1102 98-99 -0.3202497037074927 1102 100-101 -0.4973136850633679 1103 1 0.5203358104771745 1103 2 0.5728946356324514 1103 3 0.38423476947101776 1103 4 0.2417970437384298 1103 5 0.2168513021871874 1103 6 0.19177969587827448 1103 7 0.29400981102857315 1103 8 0.2722635007070906 1103 9 0.18940722313244862 1103 10-11 0.2473492369658814 1103 12-13 0.4100193077590646 1103 14-15 0.5720566007357988 1103 16-17 0.3829030727929208 1103 18-19 0.4272984792298544 1103 20-21 0.38468110315027104 1103 22-23 0.36761038861999396 1103 24-25 0.3793762637274156 1103 26-27 0.34442052976937276 1103 28-29 0.41650596480603497 1103 30-31 0.4927950349247894 1103 32-33 0.4429455115805254 1103 34-35 0.4393760006457441 1103 36-37 0.3316361533171843 1103 38-39 0.5669067331419342 1103 40-41 0.6693588922522196 1103 42-43 0.6550395852165991 1103 44-45 0.6083180801196022 1103 46-47 0.5795089688994892 1103 48-49 0.6364045000134553 1103 50-51 0.34591845624076 1103 52-53 0.4864594222492471 1103 54-55 0.5418672610827358 1103 56-57 0.5808858142224267 1103 58-59 0.5548422275413927 1103 60-61 0.5651201884689669 1103 62-63 0.5800460293616467 1103 64-65 0.5723541155483858 1103 66-67 0.39621011341458257 1103 68-69 0.3765545320181154 1103 70-71 0.44845247750695094 1103 72-73 0.3793723157028239 1103 74-75 0.5162017855509262 1103 76-77 0.3388274161681686 1103 78-79 0.5355495910813808 1103 80-81 0.5609497044770464 1103 82-83 0.6430536231458319 1103 84-85 0.6730295143921872 1103 86-87 0.5608072152007253 1103 88-89 0.6258972925149955 1103 90-91 0.23074746939977686 1103 92-93 0.48737559066102776 1103 94-95 0.5558177930017436 1103 96-97 0.36624961157049896 1103 98-99 0.3054898250883191 1103 100-101 0.34928808195233785 1104 1 0.35577698694776316 1104 2 0.4111912042599002 1104 3 0.3027519263337588 1104 4 0.1595666515815637 1104 5 0.11394689042248274 1104 6 0.19079930372141263 1104 7 0.11892402671485058 1104 8 0.08259438306003375 1104 9 0.12624545842656687 1104 10-11 -0.012148312053724908 1104 12-13 0.03702298422965811 1104 14-15 0.13101493406912823 1104 16-17 0.12990062181252426 1104 18-19 0.18834627334750564 1104 20-21 0.08341884824831425 1104 22-23 0.111605486659208 1104 24-25 0.2987941558842806 1104 26-27 0.2119144023183921 1104 28-29 0.230274347158975 1104 30-31 0.08025214276792525 1104 32-33 0.14148105079620876 1104 34-35 0.344578206528098 1104 36-37 0.3514216925328739 1104 38-39 0.2564655566713441 1104 40-41 0.315045166762026 1104 42-43 0.23291948717738364 1104 44-45 0.4284528840411781 1104 46-47 0.4866842139975276 1104 48-49 0.5618211666801258 1104 50-51 0.42517090722115114 1104 52-53 0.22130623597473686 1104 54-55 0.5162361336317574 1104 56-57 0.49200101030085364 1104 58-59 0.3633471295021806 1104 60-61 0.2889988649395505 1104 62-63 0.11756441171458931 1104 64-65 0.16690068417584314 1104 66-67 0.22630815263027415 1104 68-69 0.222406247704388 1104 70-71 0.17869757554616683 1104 72-73 0.1423808941341953 1104 74-75 0.05127531496269455 1104 76-77 0.08834334754071804 1104 78-79 0.2471856204931484 1104 80-81 0.2646261750652812 1104 82-83 0.30168720157720585 1104 84-85 0.29318882811767466 1104 86-87 0.43897510735759226 1104 88-89 0.5686730278091119 1104 90-91 0.4218259007723262 1104 92-93 0.4581537769355357 1104 94-95 0.5715347047664494 1104 96-97 0.6115376017665746 1104 98-99 0.8699996290098859 1104 100-101 0.6674682290111633 1105 1 0.4484425050378462 1105 2 0.44121315027680197 1105 3 0.2806308394052657 1105 4 0.13794916567506732 1105 5 -0.01593822261855138 1105 6 0.07909290568102989 1105 7 0.10505247390962325 1105 8 0.10526521917690701 1105 9 0.04152841261242912 1105 10-11 0.11507375878297665 1105 12-13 0.13051971869749224 1105 14-15 0.16651752422652777 1105 16-17 0.13963312359985025 1105 18-19 0.018502342051135656 1105 20-21 0.09141713124351725 1105 22-23 0.06577797678496466 1105 24-25 0.06580726342855314 1105 26-27 -0.0010059122509531448 1105 28-29 0.07301018626449007 1105 30-31 0.10780717628760783 1105 32-33 0.1989551125966642 1105 34-35 0.09008150722075925 1105 36-37 0.21589478302429654 1105 38-39 0.15944782698532833 1105 40-41 0.05869466631079945 1105 42-43 -0.06858003522572176 1105 44-45 -0.31230393123723843 1105 46-47 -7.224641549044009E-4 1105 48-49 0.06120164585924215 1105 50-51 -0.10367957125475158 1105 52-53 0.053577062715469026 1105 54-55 0.005637621214241051 1105 56-57 0.006536217689259161 1105 58-59 0.21230711098431243 1105 60-61 0.22915018697464262 1105 62-63 0.07893070509386746 1105 64-65 0.18516418727582362 1105 66-67 0.21614548400633993 1105 68-69 -0.10554368226759792 1105 70-71 0.09583890906702308 1105 72-73 0.07564753237945254 1105 74-75 -0.058299111042018126 1105 76-77 -0.15554403481808166 1105 78-79 -0.07244372182955416 1105 80-81 -0.06300987412426906 1105 82-83 0.19795070174714624 1105 84-85 0.21037447852846825 1105 86-87 0.09466125734059716 1105 88-89 0.12560832807984923 1105 90-91 -0.13656013669705658 1105 92-93 -0.19597523868744915 1105 94-95 0.030095848889370558 1105 96-97 0.031178444505346903 1105 98-99 0.06042930506440669 1105 100-101 0.5915121928309972 1106 1 0.26940472543318705 1106 2 0.36339133651221545 1106 3 0.1453436404387567 1106 4 0.024824902975964847 1106 5 -0.009774430950642454 1106 6 -0.12090358857920336 1106 7 -0.07013425055500733 1106 8 -0.03678525295859458 1106 9 0.04015135802981007 1106 10-11 -0.07951110027752151 1106 12-13 0.0013197109848661626 1106 14-15 0.08892060366834897 1106 16-17 0.08883232499233173 1106 18-19 -0.09286922604874093 1106 20-21 -0.07091457133653023 1106 22-23 -0.008791737479711514 1106 24-25 -0.10543953539281858 1106 26-27 -0.07032928694616913 1106 28-29 -0.09733476100041827 1106 30-31 -0.12764759704001705 1106 32-33 0.03726460982099411 1106 34-35 -0.1336912647501478 1106 36-37 -0.06885963114029181 1106 38-39 -0.011872592371261703 1106 40-41 0.1096044934252447 1106 42-43 -0.10215432973941319 1106 44-45 -0.15340662662526938 1106 46-47 -0.09833102376912706 1106 48-49 -0.009656716995344539 1106 50-51 0.046893529554544955 1106 52-53 0.1923584325131742 1106 54-55 -0.11704311281755508 1106 56-57 0.007016679325069219 1106 58-59 -0.06544003052312775 1106 60-61 -0.013007560797902329 1106 62-63 -0.39891932987588774 1106 64-65 -0.04815541524340006 1106 66-67 -0.13753072236254837 1106 68-69 -0.136126003172496 1106 70-71 -0.07686277175990952 1106 72-73 -0.014704847053771175 1106 74-75 0.0831226066652988 1106 76-77 0.19784592789837419 1106 78-79 -0.006258510091637248 1106 80-81 -0.0671936239687021 1106 82-83 -0.035988715563846085 1106 84-85 0.011491757793944402 1106 86-87 0.02345109349984753 1106 88-89 0.13564894207511458 1106 90-91 0.018040357962828324 1106 92-93 0.1378530466727561 1106 94-95 0.0945962020926494 1106 96-97 0.07342611450304659 1106 98-99 -0.2585607614219434 1106 100-101 -0.15920990191862927 1107 1 0.42473081687922587 1107 2 0.508598060728481 1107 3 0.28386745192940666 1107 4 0.2017362242762033 1107 5 -0.010427026878112144 1107 6 0.1611458930613736 1107 7 0.12659863945366823 1107 8 0.23454182977238958 1107 9 0.3590022935549868 1107 10-11 0.31971239317586964 1107 12-13 0.2886952840714869 1107 14-15 0.39539286834142473 1107 16-17 0.3753446540989387 1107 18-19 0.39696597282780743 1107 20-21 0.29987556537818705 1107 22-23 0.3237843642922087 1107 24-25 0.30654055630103727 1107 26-27 0.24231785691406316 1107 28-29 0.1934813969443141 1107 30-31 0.2028674581002079 1107 32-33 0.3166975122207276 1107 34-35 0.3602102676111727 1107 36-37 0.4748415790534182 1107 38-39 0.4459544284044199 1107 40-41 0.46631631862866385 1107 42-43 0.35442738931391204 1107 44-45 0.30099013517721573 1107 46-47 0.47162684662036014 1107 48-49 0.5395006907945046 1107 50-51 0.344679659825907 1107 52-53 0.30841724875372734 1107 54-55 0.41337694418133 1107 56-57 0.40743591025315595 1107 58-59 0.33804805340566446 1107 60-61 0.31533905850737654 1107 62-63 0.24542415036037113 1107 64-65 0.17005738059320663 1107 66-67 0.23759215566810354 1107 68-69 0.23806341486062976 1107 70-71 0.14053634434433704 1107 72-73 0.1781331191599307 1107 74-75 0.30867297633197666 1107 76-77 0.3981267919684264 1107 78-79 0.6312038004283735 1107 80-81 0.4108576750276214 1107 82-83 0.3425210527232956 1107 84-85 0.4668075233550546 1107 86-87 0.757540970002264 1107 88-89 0.8813554391219114 1107 90-91 0.5654361697839008 1107 92-93 0.7271967334267124 1107 94-95 0.7709726425535983 1107 96-97 0.9128689297523174 1107 98-99 0.846730222015335 1107 100-101 0.7759394103774326 1108 1 0.27147486373161556 1108 2 0.2539412332655857 1108 3 0.07021479905681716 1108 4 0.025598818886358288 1108 5 -0.04455032503175005 1108 6 -0.015468824831785355 1108 7 -0.09856785169331772 1108 8 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-1.297037153476726 2316 84-85 -1.6604869474855697 2316 86-87 -1.34204033520885 2316 88-89 -1.4067887897156268 2316 90-91 -1.5850886207073422 2316 92-93 -1.5969724323383439 2316 94-95 -1.8050429111570487 2316 96-97 -1.919648112122001 2316 98-99 -2.300048139624977 2316 100-101 -3.0553966612738748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 15.0 5 52.0 6 139.0 7 322.0 8 724.0 9 1344.0 10 2343.0 11 3705.0 12 5493.0 13 7588.0 14 9468.0 15 12360.0 16 15704.0 17 20435.0 18 17737.0 19 11156.0 20 9615.0 21 9742.0 22 10299.0 23 11552.0 24 13475.0 25 16553.0 26 20324.0 27 25031.0 28 31019.0 29 38678.0 30 47518.0 31 58784.0 32 72746.0 33 93274.0 34 121382.0 35 162204.0 36 227795.0 37 306841.0 38 207988.0 39 14330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7721272721103 19.643487779650386 15.126162369090126 21.45822257914918 2 15.295058053640131 21.55968809970359 39.9716963233213 23.17355752333498 3 19.767180035828027 26.161051950636942 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21.546250329267675 26.0889779997879 31.113750435784016 21.251021235160408 24-25 21.63349820121511 26.235202941747232 31.33558533903239 20.795713518005265 26-27 21.398860458223798 26.196927821246007 31.50468216014726 20.89952956038293 28-29 20.84497484686693 26.66092158492106 31.57184690176097 20.922256666451045 30-31 21.160757380491518 26.273703904582295 31.352969861023343 21.21256885390284 32-33 21.02108266148658 26.42673939188111 31.257722622225415 21.29445532440689 34-35 21.73845571089409 26.242813683530265 31.063266952491787 20.955463653083857 36-37 21.750323751612537 25.94845849448347 31.13566840868463 21.16554934521936 38-39 21.445920419268905 26.338655674914836 31.269612023254844 20.945811882561415 40-41 21.57037333757127 26.423391670276896 31.033734206091246 20.97250078606059 42-43 21.316537396173576 26.64935733831132 30.489527628263307 21.5445776372518 44-45 21.570336118956075 26.57686866396781 30.222653889562203 21.63014132751391 46-47 21.997595993114526 26.970282680732026 29.583559454075846 21.4485618720776 48-49 22.099522089445102 27.035598520299846 29.253904677900227 21.610974712354825 50-51 21.816357128662467 27.340303549286876 29.260725655895165 21.582613666155495 52-53 22.119962282036322 27.6734099552037 28.760165003651128 21.44646275910885 54-55 21.695561964637665 27.299548492502403 28.979739856787806 22.025149686072126 56-57 22.126291557413836 27.586574738267483 28.477909909215054 21.809223795103627 58-59 22.493528933744305 27.093358445289596 28.55588317342395 21.857229447542146 60-61 22.042483207846384 27.350442767967692 29.18063745027355 21.426436573912376 62-63 22.078459667662912 27.303796405435076 29.072591053706994 21.54515287319502 64-65 21.860376434771492 27.566648077064837 28.895954942039943 21.677020546123728 66-67 21.849026454160914 27.606704088528648 28.542292105115024 22.00197735219541 68-69 21.870702454176616 27.5571000199658 28.67363002558235 21.898567500275227 70-71 22.06372126278156 27.50080625774186 28.518883535952565 21.916588943524015 72-73 22.017232757290923 27.30396230264369 28.680107379745134 21.99869756032025 74-75 21.78386343167354 28.048822206409945 28.30517393229802 21.8621404296185 76-77 21.9441955615322 27.581924808092356 28.49961078893689 21.974268841438555 78-79 21.96611362969082 27.510935501689467 28.521536344681042 22.001414523938674 80-81 22.037147280919314 27.79632570618464 28.265643244023348 21.9008837688727 82-83 22.0624067310988 27.708958106673187 28.349951448852096 21.878683713375917 84-85 21.814078125553994 27.75193248749878 28.187632453225003 22.246356933722225 86-87 21.913957980780925 27.72421866714273 28.15137145155125 22.210451900525094 88-89 22.064103433494818 27.65634069545861 27.885254866486953 22.39430100455962 90-91 21.8661708857708 27.501406748223328 28.20660053420661 22.425821831799258 92-93 21.961559131090315 27.642382112263608 28.289573531643647 22.106485225002427 94-95 21.741534715960885 27.479605968875486 28.21512534371045 22.563733971453175 96-97 21.52101959481693 27.390787244987774 28.386483364528537 22.701709795666762 98-99 21.51736728828952 27.566962341846953 27.897575297644966 23.018095072218557 100-101 21.405395755279464 27.58502286331711 28.10489818032753 22.904683201075894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 706.0 1 562.5 2 838.5 3 1069.0 4 691.0 5 537.5 6 803.5 7 1508.5 8 2320.0 9 2752.5 10 2597.0 11 2245.0 12 2204.5 13 2298.0 14 2464.0 15 2646.0 16 2876.0 17 3171.5 18 3300.5 19 3490.0 20 3855.0 21 4300.0 22 4880.5 23 5645.0 24 6629.5 25 7984.5 26 9943.5 27 12473.0 28 14798.5 29 17253.0 30 20607.5 31 24338.5 32 28875.0 33 32993.5 34 36179.0 35 39141.5 36 43325.5 37 47673.0 38 49361.0 39 49833.5 40 50580.5 41 51705.0 42 52052.0 43 53137.0 44 55195.0 45 56769.5 46 57343.5 47 61005.5 48 73927.5 49 72735.5 50 58663.0 51 51728.0 52 47183.0 53 45251.0 54 43581.5 55 40519.5 56 36619.5 57 32791.5 58 30602.5 59 27983.5 60 23752.0 61 19774.0 62 16421.0 63 12578.5 64 8872.0 65 6622.0 66 5022.0 67 3886.5 68 2875.5 69 2084.0 70 1617.5 71 1265.5 72 1055.0 73 989.0 74 819.0 75 665.0 76 486.5 77 253.5 78 166.5 79 126.5 80 79.0 81 47.0 82 34.0 83 27.5 84 15.0 85 8.0 86 5.0 87 3.0 88 1.5 89 2.5 90 2.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0194358333872389 2 0.011631147092337623 3 0.004478302623787749 4 0.014678880822415396 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003669720205603849 10-11 2.798939139867343E-4 12-13 1.5549661888151903E-4 14-15 6.219864755260762E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010884763321706331 24-25 0.0 26-27 3.109932377630381E-5 28-29 0.0 30-31 0.0 32-33 0.0035142235867223304 34-35 5.597878279734686E-4 36-37 0.0023635486069990895 38-39 6.219864755260762E-5 40-41 0.003203230348959292 42-43 0.008770009304917673 44-45 0.0020214560454597475 46-47 0.0012128736272758484 48-49 0.008023625534286382 50-51 4.042912090919495E-4 52-53 0.0018659594265782286 54-55 0.008552314038483546 56-57 0.003203230348959292 58-59 0.0011506749797232409 60-61 0.0050691897755375205 62-63 0.002923336434972558 64-65 0.013839199080455195 66-67 0.0028922371111962542 68-69 1.8659594265782285E-4 70-71 9.640790370654181E-4 72-73 0.0 74-75 0.0 76-77 7.774830944075953E-4 78-79 0.008956605247575498 80-81 0.012750722748284562 82-83 0.010511571436390687 84-85 0.006779652583234231 86-87 0.008116923505615294 88-89 0.0236976847175435 90-91 0.01999686518816335 92-93 0.0022391513118938744 94-95 0.006997347849668357 96-97 0.008272420124496813 98-99 0.008210221476944205 100-101 0.00964079037065418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1607752.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.022232734677694 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64858218028309 46.21388572251487 2 12.443567441442251 14.440071322740403 3 3.5424933140479156 6.166301145861834 4 1.4599333521778841 3.3883437094873345 5 0.7256401817305802 2.105163175300277 6 0.4522910163335938 1.5745760768107016 7 0.2653095610751303 1.0775697169605476 8 0.2222461254504276 1.0316173132212068 9 0.15437662303534055 0.8061548715495122 >10 0.9487089771435648 10.503793628441967 >50 0.08582636850254594 3.3707259445901796 >100 0.04626850378855255 4.890590355483871 >500 0.002702474425703149 1.039858754341343 >1k 0.0016214846554218894 1.6007765925749293 >5k 4.323959081125038E-4 1.790571670121036 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9545 0.5936860908896396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8240 0.5125168558334867 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5708 0.35502988023028426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5055 0.3144141633784315 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2765 0.17197926048296006 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2436 0.15151590543815216 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2288 0.14231050560036623 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2277 0.14162632047728754 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1826 0.1135747304310615 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1799 0.1118953669471411 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1749 0.10878543456951072 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1663 0.10343635087998646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.219864755260762E-5 0.0 0.0 0.0 0.0 3 6.219864755260762E-5 0.0 0.0 0.0 0.0 4 6.219864755260762E-5 0.0 0.0 0.0 0.0 5 6.219864755260762E-5 0.0 0.0 0.0 0.0 6 1.2439729510521524E-4 0.0 0.0 0.0 0.0 7 1.2439729510521524E-4 0.0 0.0 0.0 0.0 8 1.2439729510521524E-4 0.0 0.0 0.0 0.0 9 1.2439729510521524E-4 0.0 0.0 0.0 0.0 10-11 1.2439729510521524E-4 0.0 0.0 0.0 0.0 12-13 1.2439729510521524E-4 0.0 0.0 0.0 6.219864755260762E-5 14-15 1.2439729510521524E-4 0.0 0.0 0.0 6.219864755260762E-5 16-17 1.2439729510521524E-4 0.0 0.0 3.109932377630381E-5 6.219864755260762E-5 18-19 1.2439729510521524E-4 0.0 0.0 6.219864755260762E-5 6.219864755260762E-5 20-21 1.2439729510521524E-4 0.0 0.0 9.329797132891143E-5 1.2439729510521524E-4 22-23 1.8659594265782285E-4 0.0 0.0 1.8659594265782285E-4 1.2439729510521524E-4 24-25 1.8659594265782285E-4 0.0 0.0 4.975891804208609E-4 1.2439729510521524E-4 26-27 1.8659594265782285E-4 0.0 0.0 0.0010262776846180258 1.2439729510521524E-4 28-29 2.487945902104305E-4 0.0 0.0 0.001648264160144102 1.2439729510521524E-4 30-31 2.487945902104305E-4 0.0 0.0 0.0035142235867223304 1.2439729510521524E-4 32-33 2.798939139867343E-4 0.0 0.0 0.0055667789559583816 1.2439729510521524E-4 34-35 3.1099323776303807E-4 0.0 0.0 0.007183943792326179 1.2439729510521524E-4 36-37 3.731918853156457E-4 0.0 0.0 0.009547492399325268 1.2439729510521524E-4 38-39 4.3539053286825334E-4 0.0 0.0 0.013683702461573674 1.2439729510521524E-4 40-41 4.3539053286825334E-4 0.0 0.0 0.01803760779025621 1.2439729510521524E-4 42-43 4.975891804208609E-4 0.0 0.0 0.021614030024531147 1.5549661888151903E-4 44-45 5.597878279734685E-4 0.0 0.0 0.023728784041319805 1.8659594265782285E-4 46-47 5.908871517497723E-4 0.0 0.0 0.02718080898048953 2.1769526643412667E-4 48-49 6.219864755260761E-4 0.0 0.0 0.03246769402246118 2.798939139867343E-4 50-51 6.219864755260761E-4 0.0 0.0 0.03800337365464325 3.1099323776303807E-4 52-53 6.219864755260761E-4 0.0 0.0 0.04926132886166523 3.731918853156457E-4 54-55 6.219864755260761E-4 0.0 0.0 0.056227577387557286 4.3539053286825334E-4 56-57 6.841851230786838E-4 0.0 0.0 0.07323890749319546 4.3539053286825334E-4 58-59 6.841851230786838E-4 0.0 0.0 0.10508461504013056 4.3539053286825334E-4 60-61 6.841851230786838E-4 0.0 0.0 0.13117694768844945 4.3539053286825334E-4 62-63 6.841851230786838E-4 0.0 0.0 0.14781508590877201 4.3539053286825334E-4 64-65 6.841851230786838E-4 0.0 0.0 0.17975409142703602 4.3539053286825334E-4 66-67 6.841851230786838E-4 0.0 0.0 0.24195273897964364 4.6648985664455716E-4 68-69 6.841851230786838E-4 0.0 0.0 0.3284088590777682 4.97589180420861E-4 70-71 6.841851230786838E-4 0.0 0.0 0.39968850917305654 4.97589180420861E-4 72-73 6.841851230786838E-4 0.0 0.0 0.4873264035746807 4.97589180420861E-4 74-75 6.841851230786838E-4 0.0 0.0 0.5937482895371924 5.597878279734685E-4 76-77 6.841851230786838E-4 0.0 0.0 0.6581238897541413 5.597878279734685E-4 78-79 6.841851230786838E-4 0.0 0.0 0.7357478018997955 5.597878279734685E-4 80-81 0.001119575655946937 0.0 0.0 0.8462125999532266 5.597878279734685E-4 82-83 0.001119575655946937 0.0 0.0 0.9521679960590936 5.597878279734685E-4 84-85 0.0011506749797232409 0.0 0.0 1.0911508740153955 5.597878279734685E-4 86-87 0.0011817743034995447 0.0 0.0 1.3194510098572416 6.219864755260761E-4 88-89 0.0011817743034995447 0.0 0.0 1.600557797471252 6.841851230786838E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 155 1.3587851E-9 30.643559 2 GTACCTG 1730 0.0 30.234646 1 TACCTGG 1790 0.0 28.127018 2 TACCGTA 70 0.008401062 27.137217 7 GTACCGT 110 9.492341E-5 25.903704 6 TAACGCA 190 1.19289325E-8 24.997911 4 CCGTATG 135 1.3453271E-5 24.624512 9 GTACATG 9660 0.0 23.000027 1 GAGTACT 9110 0.0 22.41506 12-13 TATCACG 130 2.943257E-4 21.921928 2 TACATGG 9930 0.0 21.907211 2 ACATGGG 9715 0.0 21.657995 3 GTACTTT 9785 0.0 20.723202 14-15 AGTACTT 9500 0.0 19.695292 12-13 GGTATCA 15560 0.0 19.089981 1 CATGGGA 6330 0.0 18.833342 4 GTGTAGC 845 0.0 18.740524 1 ACTTTTT 11225 0.0 18.360868 16-17 CATGGGG 3320 0.0 18.311724 4 ACCTGGG 2620 0.0 18.309542 3 >>END_MODULE