FastQCFastQC Report
Thu 26 May 2016
SRR936481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588392
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.4759530393001224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60470.3806994746888677No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48420.30483658945650693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.22752569894585215No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32110.20215412820009165No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23430.14750766813229982No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21590.13592362590594764No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20960.13195735057844662No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19210.12093991911316603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG953.4774683E-530.0179232
GCGTATC700.00836938927.1582161
GTACATG101600.026.2426971
TACATGG103050.025.3207932
GTACCTG15900.024.809631
TACCTGG15100.024.5510832
ACATGGG103050.024.0293873
GAGTACT65950.023.17145712-13
ATCACGC1302.9303358E-421.9361723
CATGGGA68200.021.325724
AGTACTT67650.021.00828412-13
GTACTTT73350.020.8334514-15
GTATAGG5100.019.569891
GTATCAA177350.019.3752081
CATGGGG32650.019.2157544
ACTTTTT84600.018.42620716-17
GTACACG2653.98466E-717.934671
ATGGGAT25500.017.8913425
TCACGCA2202.9026087E-517.283594
CAACGCA200200.017.1389835
Produced by FastQC (version 0.11.5)