##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936481_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1588392 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.232261305773385 31.0 31.0 34.0 28.0 34.0 2 31.240816498698056 31.0 31.0 34.0 28.0 34.0 3 31.28403945625513 31.0 31.0 34.0 27.0 34.0 4 34.918348871059536 37.0 35.0 37.0 32.0 37.0 5 34.90561775682577 37.0 35.0 37.0 32.0 37.0 6 35.044187454985924 37.0 35.0 37.0 32.0 37.0 7 34.90053085132637 37.0 35.0 37.0 32.0 37.0 8 34.84736135664244 37.0 35.0 37.0 32.0 37.0 9 36.282167122473545 39.0 37.0 39.0 32.0 39.0 10-11 36.194190099169475 39.0 35.0 39.0 32.0 39.0 12-13 36.074164626867926 39.0 35.0 39.0 30.5 39.0 14-15 37.07009604681967 39.0 36.0 41.0 31.0 41.0 16-17 36.955000402923204 39.0 36.0 41.0 31.0 41.0 18-19 36.94391308946406 39.0 36.0 41.0 31.0 41.0 20-21 36.866877634740035 39.0 36.0 41.0 30.5 41.0 22-23 36.72698584480405 39.0 36.0 41.0 30.0 41.0 24-25 36.86400806601897 39.0 36.0 41.0 30.0 41.0 26-27 36.84082581629724 39.0 36.0 41.0 30.0 41.0 28-29 36.73801775632212 39.0 36.0 41.0 30.0 41.0 30-31 36.5473031846043 39.0 36.0 41.0 30.0 41.0 32-33 36.349356141305165 39.0 35.0 40.5 30.0 41.0 34-35 36.11502828017265 39.0 35.0 40.0 29.5 41.0 36-37 35.91191437630006 38.0 35.0 40.0 29.0 41.0 38-39 35.69517977929881 38.0 35.0 40.0 27.5 41.0 40-41 35.51767825574544 38.0 35.0 40.0 27.0 41.0 42-43 35.278509020443316 38.0 34.0 40.0 26.5 41.0 44-45 34.98120300278521 38.0 34.0 40.0 25.5 41.0 46-47 34.641971880996635 38.0 34.0 40.0 24.0 41.0 48-49 34.4788994152577 38.0 33.5 40.0 23.5 41.0 50-51 33.55433639806799 37.5 32.0 39.5 22.5 40.5 52-53 33.68942868007394 37.5 33.0 39.5 23.0 40.5 54-55 34.11421550851427 38.0 33.0 40.0 23.0 41.0 56-57 34.035722604999265 38.0 33.0 40.0 22.0 41.0 58-59 33.784405549763534 38.0 33.0 40.0 21.5 41.0 60-61 33.470728888083045 37.0 33.0 40.0 20.0 41.0 62-63 33.14076972183189 37.0 32.0 40.0 18.5 41.0 64-65 32.80483784859153 36.0 32.0 39.5 18.0 41.0 66-67 32.404489571843726 36.0 31.5 39.0 15.0 41.0 68-69 32.00456751230175 35.5 31.0 39.0 13.0 40.0 70-71 31.564796347501122 35.0 31.0 38.5 9.5 40.0 72-73 31.165240381467548 35.0 30.5 37.5 7.5 40.0 74-75 30.744879412638696 35.0 30.0 37.0 7.0 39.0 76-77 30.30369140615163 34.5 30.0 36.5 2.0 39.0 78-79 29.86006760295947 34.0 29.0 36.0 2.0 38.5 80-81 29.36925582601776 34.0 29.0 35.5 2.0 37.0 82-83 28.880596540400607 34.0 29.0 35.0 2.0 37.0 84-85 28.54708535424505 34.0 28.0 35.0 2.0 36.5 86-87 28.219014575747046 34.0 27.5 35.0 2.0 36.0 88-89 27.802137318747512 34.0 26.5 35.0 2.0 36.0 90-91 27.38187928420692 33.0 26.0 35.0 2.0 35.0 92-93 27.00826527708525 33.0 25.5 35.0 2.0 35.0 94-95 26.542779112460906 33.0 25.0 35.0 2.0 35.0 96-97 25.946707110083658 33.0 23.5 35.0 2.0 35.0 98-99 25.236034618658366 32.5 19.0 35.0 2.0 35.0 100-101 22.747770386655183 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4239164992591853 1101 2 0.49640200040468585 1101 3 0.47291480913198214 1101 4 0.5772088080823394 1101 5 0.5211848402576535 1101 6 0.5175391099867213 1101 7 0.6124627145408752 1101 8 0.6573211365444322 1101 9 0.8741154346003626 1101 10-11 0.7255642751591296 1101 12-13 0.8794159428375821 1101 14-15 0.8936216100405758 1101 16-17 1.0462616193287673 1101 18-19 1.0942755517529932 1101 20-21 1.1487345196665117 1101 22-23 1.0473584391596589 1101 24-25 1.0152156031285742 1101 26-27 0.9394302765405129 1101 28-29 0.9049026925932893 1101 30-31 1.072449829041858 1101 32-33 0.8881841242664237 1101 34-35 0.8693505299153514 1101 36-37 0.7598081826009064 1101 38-39 0.8578660548221251 1101 40-41 0.8777722511373298 1101 42-43 1.1018220296350734 1101 44-45 0.964064863776045 1101 46-47 0.983890619970289 1101 48-49 1.0924049463284788 1101 50-51 0.8350916852869332 1101 52-53 1.0453624874832812 1101 54-55 1.1942343690513937 1101 56-57 1.2567934145972757 1101 58-59 1.0269849960022128 1101 60-61 1.0149085811137937 1101 62-63 0.9918513727209657 1101 64-65 0.9887183648828355 1101 66-67 1.0020915861564461 1101 68-69 0.9298286505909203 1101 70-71 1.0227757754065685 1101 72-73 0.7812104498798682 1101 74-75 0.8728769491185382 1101 76-77 0.768822623041661 1101 78-79 0.7309807459862796 1101 80-81 0.8236350452287056 1101 82-83 0.7757898262027574 1101 84-85 0.8874491338751973 1101 86-87 0.6954937518843991 1101 88-89 0.6322538854755884 1101 90-91 0.4543264834575176 1101 92-93 0.5030716669360977 1101 94-95 0.8891671951266744 1101 96-97 0.7424062372603792 1101 98-99 0.6344582928731057 1101 100-101 1.2548761718277746 1102 1 0.4619463259484071 1102 2 0.4335576928498668 1102 3 0.39822121492815654 1102 4 0.37107510663648213 1102 5 0.49470467684123065 1102 6 0.3477001326160689 1102 7 0.43293604766142835 1102 8 0.3398328661413146 1102 9 0.5607018027341653 1102 10-11 0.6074683797201033 1102 12-13 0.6617118318931574 1102 14-15 0.5536960969704623 1102 16-17 0.447084087491298 1102 18-19 0.6094925093987698 1102 20-21 0.7265450348112594 1102 22-23 0.5672325255277926 1102 24-25 0.42508522107885227 1102 26-27 0.49683189155440033 1102 28-29 0.5925435275490756 1102 30-31 0.5120162728195865 1102 32-33 0.45584255361755055 1102 34-35 0.45511243505723087 1102 36-37 0.4574337371634769 1102 38-39 0.3656389075877584 1102 40-41 0.3480373240682937 1102 42-43 0.3833126060609757 1102 44-45 0.25548749631251155 1102 46-47 0.3315008143464695 1102 48-49 0.5189158301176988 1102 50-51 0.5008841512152387 1102 52-53 0.559251487196029 1102 54-55 0.6448316209744078 1102 56-57 0.6386109435621989 1102 58-59 0.7471662860982846 1102 60-61 0.5456111999258368 1102 62-63 0.6067070667620058 1102 64-65 0.5329158051177458 1102 66-67 0.6014542381624466 1102 68-69 0.4237087134679456 1102 70-71 0.47478556602927924 1102 72-73 0.4113088348659808 1102 74-75 0.20683293520260548 1102 76-77 0.5098091858708074 1102 78-79 0.3365646724488869 1102 80-81 0.45959244365199226 1102 82-83 0.3763114740914304 1102 84-85 0.4962413444653464 1102 86-87 0.5913125894574094 1102 88-89 0.6112162151171567 1102 90-91 0.6893650634581583 1102 92-93 0.6135682597674794 1102 94-95 0.7442540101022566 1102 96-97 0.7480494101562307 1102 98-99 0.9698132528169303 1102 100-101 0.9924162678987578 1103 1 0.33614059695824494 1103 2 0.5848365776095115 1103 3 0.4783730842883429 1103 4 0.487586783077397 1103 5 0.5146004820532681 1103 6 0.43501467565816654 1103 7 0.3935308118829042 1103 8 0.46823561480059084 1103 9 0.5726204457894681 1103 10-11 0.6379915937904244 1103 12-13 0.59801113630391 1103 14-15 0.725731035347728 1103 16-17 0.7808066674323655 1103 18-19 0.730908156997522 1103 20-21 0.8398552392758205 1103 22-23 0.9010879815858175 1103 24-25 0.8699540777124142 1103 26-27 0.6859109681611173 1103 28-29 0.48387115908015943 1103 30-31 0.618581578367646 1103 32-33 0.7448454274943686 1103 34-35 0.6099033466371679 1103 36-37 0.6452391942745592 1103 38-39 0.6642138399530495 1103 40-41 0.5033779292098686 1103 42-43 0.5042670165332979 1103 44-45 0.5624696193052472 1103 46-47 0.6103729373583491 1103 48-49 0.4860889194491236 1103 50-51 0.6247478593779334 1103 52-53 0.7543228171634198 1103 54-55 0.6107508116171729 1103 56-57 0.6191761840124457 1103 58-59 0.4656871872817234 1103 60-61 0.3114503585557955 1103 62-63 0.4492580584505461 1103 64-65 0.5704896863448781 1103 66-67 0.35526466185345384 1103 68-69 0.4156986194781922 1103 70-71 0.4725134610150228 1103 72-73 0.3752909827490569 1103 74-75 0.22509229636333927 1103 76-77 0.14263915996571086 1103 78-79 0.4473050451529943 1103 80-81 0.4270121388027803 1103 82-83 0.4170739991971679 1103 84-85 0.38855310170412594 1103 86-87 0.4183450292275772 1103 88-89 0.5553337664439049 1103 90-91 0.8067739408847281 1103 92-93 0.7457646790050312 1103 94-95 0.6372234466807178 1103 96-97 0.6278436244926837 1103 98-99 0.7614560343314487 1103 100-101 1.5220991458346198 1104 1 0.3790823985685634 1104 2 0.4257884482077081 1104 3 0.4212745829283975 1104 4 0.5143334702783164 1104 5 0.5064340645595209 1104 6 0.4767023569089517 1104 7 0.5440699110177079 1104 8 0.4902270018231789 1104 9 0.5798130624654476 1104 10-11 0.7539335575132498 1104 12-13 0.6906460499339744 1104 14-15 0.9612981439210699 1104 16-17 0.670163756252002 1104 18-19 0.6469198065823392 1104 20-21 0.838452140525284 1104 22-23 0.6686770008651948 1104 24-25 0.8548931879549215 1104 26-27 0.4886245513878791 1104 28-29 0.6769287660594046 1104 30-31 0.6518619249281556 1104 32-33 0.3810495781749452 1104 34-35 0.3905790105479241 1104 36-37 0.5223569765154323 1104 38-39 0.4788843462844383 1104 40-41 0.664217551908024 1104 42-43 0.7374534729340425 1104 44-45 0.46266985827109863 1104 46-47 0.7252797451829807 1104 48-49 0.8172541856438613 1104 50-51 0.8227868609450226 1104 52-53 1.0238149697173 1104 54-55 1.0504487119600086 1104 56-57 0.8904136728296876 1104 58-59 1.0827226598997228 1104 60-61 1.364617068752672 1104 62-63 1.2415260799574739 1104 64-65 1.0721997481277583 1104 66-67 1.1239293651575508 1104 68-69 0.9863826742121802 1104 70-71 1.223953710548198 1104 72-73 1.0879020973626048 1104 74-75 0.9722570161788902 1104 76-77 1.1291781930546705 1104 78-79 1.0555322173104713 1104 80-81 1.1660494682093905 1104 82-83 1.0379101094576875 1104 84-85 1.170891732213704 1104 86-87 1.1933386873262997 1104 88-89 1.3313486958048841 1104 90-91 1.3950856667224585 1104 92-93 1.3287178735220273 1104 94-95 1.336257076019475 1104 96-97 1.3620357788478081 1104 98-99 1.898317641286674 1104 100-101 2.2704526906947535 1105 1 0.3517314637824107 1105 2 0.36064153661077114 1105 3 0.46599560222946224 1105 4 0.4112930870509146 1105 5 0.540295325065685 1105 6 0.4073355202811655 1105 7 0.40713625405977893 1105 8 0.5317525192732973 1105 9 0.5422484539580736 1105 10-11 0.47673395796958573 1105 12-13 0.40580256549822025 1105 14-15 0.287229146691665 1105 16-17 0.4164662288370451 1105 18-19 0.3969452716471764 1105 20-21 0.40079498469655306 1105 22-23 0.2897284606682291 1105 24-25 0.6854886630311725 1105 26-27 0.6358504599813912 1105 28-29 0.6017369777424832 1105 30-31 0.4683271993105933 1105 32-33 0.32389869693818696 1105 34-35 0.4746675649471257 1105 36-37 0.5271625980376271 1105 38-39 0.49578587360627324 1105 40-41 0.557823055854378 1105 42-43 0.6776513146606575 1105 44-45 0.45143321651653423 1105 46-47 0.6494324133397171 1105 48-49 0.7622314562545611 1105 50-51 0.8524260137004873 1105 52-53 0.6910884325004645 1105 54-55 0.69571308298746 1105 56-57 0.9507877037020762 1105 58-59 0.9087249648518778 1105 60-61 0.7955157963725199 1105 62-63 0.7920975160125181 1105 64-65 0.9776457796520575 1105 66-67 0.9315822719583196 1105 68-69 0.8687107923002344 1105 70-71 0.8734001727958791 1105 72-73 0.8966907460552953 1105 74-75 0.7896703185467047 1105 76-77 0.6549415629412891 1105 78-79 0.7202759077949743 1105 80-81 0.5569651337352219 1105 82-83 0.5636283698008704 1105 84-85 0.849661580412814 1105 86-87 0.765625275531459 1105 88-89 0.7815754076392984 1105 90-91 0.7460371870595885 1105 92-93 0.8465914387475131 1105 94-95 0.8572257379831107 1105 96-97 0.9396693030963696 1105 98-99 0.8655200929176061 1105 100-101 1.3075698416341979 1106 1 0.18882347964028057 1106 2 0.1595497033551858 1106 3 0.3006506622385636 1106 4 0.3057446881733483 1106 5 0.16255718244474338 1106 6 0.19730830112847286 1106 7 0.40277377141055837 1106 8 0.2992017365800024 1106 9 0.7451103167581365 1106 10-11 0.48477309477435426 1106 12-13 0.5111271695865156 1106 14-15 0.682462513834956 1106 16-17 0.7649131108317491 1106 18-19 0.6799032003666596 1106 20-21 0.5200285686800541 1106 22-23 0.5421647032268311 1106 24-25 0.38501803418029823 1106 26-27 0.10269067014520061 1106 28-29 0.4290299916359501 1106 30-31 0.42720441639474416 1106 32-33 0.1943805387104547 1106 34-35 0.29586069967194817 1106 36-37 0.2054926288374972 1106 38-39 0.29410825080267244 1106 40-41 0.24995881779806695 1106 42-43 0.003626881619346989 1106 44-45 0.12661959923664767 1106 46-47 0.19848198985934573 1106 48-49 -0.04484192133130449 1106 50-51 -0.05563498544784551 1106 52-53 0.1837019561966784 1106 54-55 0.22836162209787148 1106 56-57 0.26766614621873686 1106 58-59 0.3009996043906895 1106 60-61 0.42005697247387985 1106 62-63 0.47961811226603146 1106 64-65 0.49007218593927604 1106 66-67 0.55985877033131 1106 68-69 0.3960902569616991 1106 70-71 0.4365691498093831 1106 72-73 0.37862078798249144 1106 74-75 0.34445207315491544 1106 76-77 0.41399731655374694 1106 78-79 0.5886271518443493 1106 80-81 0.44262775176690994 1106 82-83 0.5765667401040311 1106 84-85 0.6016236633342231 1106 86-87 0.772620155534657 1106 88-89 0.81529513025502 1106 90-91 0.9651265794362267 1106 92-93 0.9717676214182731 1106 94-95 1.053440271412878 1106 96-97 1.338590202070229 1106 98-99 1.560444106116325 1106 100-101 2.11252813555825 1107 1 0.24194156290958801 1107 2 0.22894434953683174 1107 3 -0.022532025639325326 1107 4 0.20494711697908485 1107 5 0.33494555051863273 1107 6 0.271756880821421 1107 7 0.34332287445692344 1107 8 0.5733510507430921 1107 9 0.3030554243447483 1107 10-11 0.44771945990926554 1107 12-13 0.2755178401264118 1107 14-15 0.314418037568565 1107 16-17 0.49935503635666834 1107 18-19 0.5457324304684121 1107 20-21 0.5970992483995303 1107 22-23 0.7153664349620144 1107 24-25 0.6501388052655628 1107 26-27 0.23236075897181507 1107 28-29 0.4093604567499156 1107 30-31 0.4371276939062412 1107 32-33 0.18985405295412505 1107 34-35 0.28300342951169455 1107 36-37 0.32316027069187214 1107 38-39 0.19869439698999258 1107 40-41 0.24022253823493145 1107 42-43 0.30219465280679714 1107 44-45 0.34498159354654945 1107 46-47 0.2637533914906811 1107 48-49 0.13278307742147177 1107 50-51 0.19573326494980137 1107 52-53 0.4502459416579754 1107 54-55 0.41337174957646283 1107 56-57 0.5615245624510479 1107 58-59 0.5715462405091998 1107 60-61 0.3623430748620464 1107 62-63 0.2822896884746555 1107 64-65 0.5298198298855468 1107 66-67 0.5207445453784558 1107 68-69 0.42961828377379874 1107 70-71 0.4459147464463804 1107 72-73 0.4545538251697927 1107 74-75 0.39419387147995266 1107 76-77 0.46227065628893627 1107 78-79 0.49754177584085113 1107 80-81 0.6467560540354178 1107 82-83 0.86014095730172 1107 84-85 0.6570177197928047 1107 86-87 0.563407986320609 1107 88-89 0.5339291059414997 1107 90-91 0.47332042698008436 1107 92-93 0.4836733868385501 1107 94-95 0.6370243976094869 1107 96-97 0.5610933321784835 1107 98-99 1.0174844670516094 1107 100-101 1.3653724139199177 1108 1 0.017762828275369458 1108 2 0.045637249812006786 1108 3 0.08368752242063593 1108 4 0.005815245801386482 1108 5 0.17584060446533556 1108 6 -0.04503365518009872 1108 7 -0.056682743404628866 1108 8 0.09552654393888105 1108 9 0.06614648006615198 1108 10-11 -0.04657857982931546 1108 12-13 0.2906239652537792 1108 14-15 0.18124629108032053 1108 16-17 0.22682800378220946 1108 18-19 0.25371006461093515 1108 20-21 0.11329882597694763 1108 22-23 0.3107555243303466 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-3.895074067571457 2316 80-81 -3.1900739815700696 2316 82-83 -3.0484248262683273 2316 84-85 -3.2119582747076265 2316 86-87 -3.850821165501067 2316 88-89 -4.190125909207421 2316 90-91 -4.20150050540472 2316 92-93 -4.794350053036375 2316 94-95 -4.958297410575167 2316 96-97 -5.637291357798116 2316 98-99 -6.517620057308292 2316 100-101 -6.566170699309588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3882.0 3 1613.0 4 1546.0 5 1739.0 6 1979.0 7 2294.0 8 2801.0 9 3747.0 10 4780.0 11 6062.0 12 7788.0 13 9259.0 14 10427.0 15 12644.0 16 15169.0 17 18538.0 18 15768.0 19 11510.0 20 11465.0 21 12230.0 22 13546.0 23 15121.0 24 17569.0 25 19897.0 26 23593.0 27 27188.0 28 32398.0 29 38696.0 30 46276.0 31 56331.0 32 69417.0 33 87177.0 34 112981.0 35 151194.0 36 205100.0 37 268566.0 38 215345.0 39 32747.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.291772970229125 18.86458663317855 14.238893783768635 21.60474661282369 2 15.193234828868224 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27.096097492335602 29.330900201183557 21.925135541557104 46-47 22.13266573616061 27.35231364223868 28.733246326610146 21.781774294990566 48-49 22.04904705917535 27.471421218889148 28.519412165597174 21.960119556338327 50-51 21.81841246976883 27.725511218781584 28.668984953589664 21.787091357859914 52-53 22.08816112263789 27.99779391002062 28.175873975850745 21.73817099149075 54-55 21.66045184311886 27.627721313565523 28.4681312558141 22.243695587501517 56-57 22.0655305188856 27.95937321919382 27.89202922962128 22.083067032299297 58-59 22.39629998784796 27.465242338706886 28.022000855050116 22.116456818395037 60-61 22.050471597133896 27.650577375930286 28.664322323103853 21.63462870383196 62-63 22.054858861142836 27.674064121629876 28.546635572507356 21.724441444719933 64-65 21.782129909408354 27.99089519177105 28.34751382137212 21.879461077448475 66-67 21.799922431520002 27.986040192057544 27.944328245630164 22.269709130792297 68-69 21.80094652439416 27.94863253923177 28.232646528046264 22.017774408327803 70-71 22.018999601145 27.840928944941957 27.96955732131878 22.17051413259426 72-73 21.9504862758307 27.65739235274397 28.20727202328206 22.184849348143274 74-75 21.65381026673567 28.441353535887743 27.905880071572746 21.99895612580384 76-77 21.96686156346203 27.846505315667642 28.020939678953884 22.165693441916442 78-79 21.928970731655106 27.922789291463907 28.049685733767422 22.098554243113565 80-81 21.988013063607326 28.19297469067748 27.743102537480425 22.07590970823477 82-83 21.989858268866687 28.089220538579568 27.930307393679616 21.990613798874133 84-85 21.831181915496504 28.129998110950194 27.76509665638184 22.273723317171463 86-87 21.872611472988474 28.162331063852292 27.79902311999708 22.166034343162156 88-89 21.990802025707612 27.981384947092288 27.49412967261218 22.53368335458792 90-91 21.82459847192522 27.908440171915494 27.849258832421427 22.417702523737855 92-93 22.029708815075992 28.10735623911248 27.839019163920486 22.02391578189104 94-95 21.683343869396474 27.867593168601356 27.90889789421457 22.540165067787605 96-97 21.517856805557827 27.777214612849647 28.050062773650936 22.65486580794159 98-99 21.577526713019505 28.021081379426732 27.5862991814105 22.81509272614326 100-101 21.61854669249244 27.866486083588214 27.76357766106286 22.751389562856485 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 412.0 1 305.5 2 458.5 3 651.0 4 520.0 5 501.5 6 748.0 7 1325.0 8 2048.0 9 2438.0 10 2214.5 11 1873.5 12 1919.0 13 2083.5 14 2219.0 15 2314.5 16 2407.0 17 2548.5 18 2721.5 19 2969.5 20 3287.5 21 3781.5 22 4345.0 23 5023.0 24 5997.0 25 7385.0 26 9281.0 27 11740.5 28 13939.5 29 16405.0 30 19932.5 31 23649.0 32 28149.0 33 32244.5 34 35976.5 35 39342.5 36 43196.0 37 47285.5 38 48663.0 39 49273.5 40 49897.5 41 50770.0 42 51781.5 43 52922.5 44 54767.0 45 56600.5 46 57161.0 47 60507.5 48 72313.5 49 71704.0 50 58938.5 51 52142.0 52 47675.5 53 45722.0 54 43996.5 55 40616.0 56 36731.5 57 33056.0 58 30778.5 59 27896.0 60 23691.0 61 19946.5 62 16619.5 63 12662.0 64 9007.0 65 6863.0 66 5194.0 67 3970.5 68 3025.5 69 2196.5 70 1651.5 71 1332.5 72 1126.5 73 1003.5 74 807.0 75 626.0 76 475.0 77 292.5 78 185.0 79 127.5 80 77.5 81 48.5 82 32.0 83 23.5 84 14.0 85 10.0 86 6.0 87 5.0 88 5.5 89 3.5 90 3.5 91 3.5 92 2.5 93 1.5 94 1.5 95 1.5 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03557056444504883 2 0.02367173846254577 3 0.022727387194093146 4 0.051498622506283086 5 0.057290643619459174 6 0.050176530730449406 7 0.05112088199890204 8 0.04274763408528877 9 0.044573379870963846 10-11 0.051813406262433956 12-13 0.0469342580420954 14-15 0.04082745317276844 16-17 0.05102644687205678 18-19 0.054835330321482356 20-21 0.01202473948496341 22-23 0.021908949428100875 24-25 0.0064530670010929285 26-27 0.010513777455439213 28-29 0.00607532649371188 30-31 0.00966386131383185 32-33 0.004564364464187682 34-35 0.008089942533077477 36-37 0.009349077557680975 38-39 0.011867347606887972 40-41 0.00223496466867121 42-43 0.002046094414980685 44-45 0.002612705176052259 46-47 5.666107610715743E-4 48-49 0.001227656648988411 50-51 4.09218882996137E-4 52-53 0.0038718402006557577 54-55 0.007523331772005903 56-57 0.017439020090758452 58-59 0.01139517197266166 60-61 0.010828561211590086 62-63 0.006169761620557142 64-65 3.147837561508746E-5 66-67 0.007428896645160641 68-69 0.016746495827226527 70-71 0.006012369742481705 72-73 0.0028015754297427836 74-75 0.004910626595953644 76-77 0.007271504767085203 78-79 0.005603150859485567 80-81 0.010261950450518512 82-83 0.006484545376708017 84-85 0.018383371359211077 86-87 0.004406972586112244 88-89 0.006767850757243805 90-91 0.0033681861908143582 92-93 0.017470498466373538 94-95 0.012339523241114285 96-97 0.009191685679605538 98-99 0.022381125062327183 100-101 0.03604274007927514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1588392.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.682090968051185 #Duplication Level Percentage of deduplicated Percentage of total 1 81.30089391583127 49.335082403843515 2 11.514383991597716 13.9743379363841 3 3.237867961107278 5.894417944753508 4 1.2928944564594687 3.138221560758503 5 0.6604799187077998 2.003965125479888 6 0.3863857823155089 1.4068018314738802 7 0.2782403834875922 1.1818945783245665 8 0.192065033687058 0.9323926276783896 9 0.14384190844215838 0.7855764995794631 >10 0.8702256381717972 10.111030552743923 >50 0.07868384701081399 3.2433930384413148 >100 0.040071913618080726 4.361745649720827 >500 0.0021913221271814233 0.8568591834348525 >1k 0.0015652300908438738 1.9126728122105494 >5k 2.0869734544584986E-4 0.8616082551727786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7560 0.4759530393001224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6047 0.3806994746888677 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4842 0.30483658945650693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3614 0.22752569894585215 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3211 0.20215412820009165 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2343 0.14750766813229982 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2159 0.13592362590594764 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2096 0.13195735057844662 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1921 0.12093991911316603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.295675123017492E-5 0.0 0.0 0.0 0.0 8 6.295675123017492E-5 0.0 0.0 0.0 0.0 9 6.295675123017492E-5 0.0 0.0 0.0 0.0 10-11 6.295675123017492E-5 0.0 0.0 0.0 0.0 12-13 1.2591350246034984E-4 0.0 0.0 6.295675123017492E-5 0.0 14-15 1.2591350246034984E-4 0.0 0.0 6.295675123017492E-5 0.0 16-17 1.2591350246034984E-4 0.0 0.0 6.295675123017492E-5 0.0 18-19 1.8887025369052475E-4 0.0 0.0 6.295675123017492E-5 0.0 20-21 1.8887025369052475E-4 0.0 0.0 9.443512684526237E-5 0.0 22-23 1.8887025369052475E-4 0.0 0.0 1.2591350246034984E-4 0.0 24-25 2.518270049206997E-4 0.0 0.0 1.2591350246034984E-4 0.0 26-27 2.518270049206997E-4 0.0 0.0 2.518270049206997E-4 0.0 28-29 2.518270049206997E-4 0.0 0.0 8.499161416073614E-4 0.0 30-31 2.518270049206997E-4 0.0 0.0 0.0018887025369052476 3.147837561508746E-5 32-33 2.518270049206997E-4 6.295675123017492E-5 0.0 0.004060710454346282 6.295675123017492E-5 34-35 2.8330538053578715E-4 6.295675123017492E-5 0.0 0.00594941299125153 6.295675123017492E-5 36-37 3.147837561508746E-4 6.295675123017492E-5 0.0 0.009474991060141325 6.295675123017492E-5 38-39 3.147837561508746E-4 6.295675123017492E-5 0.0 0.014637444661015669 6.295675123017492E-5 40-41 3.147837561508746E-4 6.295675123017492E-5 0.0 0.019768419886274924 6.295675123017492E-5 42-43 3.147837561508746E-4 6.295675123017492E-5 0.0 0.024930873487149268 6.295675123017492E-5 44-45 3.147837561508746E-4 6.295675123017492E-5 0.0 0.028173146175503275 6.295675123017492E-5 46-47 4.4069725861122443E-4 6.295675123017492E-5 0.0 0.032674553888460786 6.295675123017492E-5 48-49 5.036540098413994E-4 6.295675123017492E-5 0.0 0.038183269621101086 6.295675123017492E-5 50-51 5.036540098413994E-4 6.295675123017492E-5 0.0 0.044793728500269456 6.295675123017492E-5 52-53 5.036540098413994E-4 6.295675123017492E-5 0.0 0.05832943001475706 6.295675123017492E-5 54-55 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.06597867528922333 6.295675123017492E-5 56-57 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.0878561463417091 1.2591350246034984E-4 58-59 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.12125470286931689 1.2591350246034984E-4 60-61 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.14867236803005807 1.2591350246034984E-4 62-63 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.1658280827402807 1.8887025369052475E-4 64-65 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.19853411500435658 1.8887025369052475E-4 66-67 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.26548861993764766 1.8887025369052475E-4 68-69 5.036540098413994E-4 1.2591350246034984E-4 0.0 0.3546353796795753 1.8887025369052475E-4 70-71 5.666107610715743E-4 1.2591350246034984E-4 0.0 0.43427566998574657 1.8887025369052475E-4 72-73 5.666107610715743E-4 1.2591350246034984E-4 0.0 0.5213763353126936 2.2034862930561222E-4 74-75 5.666107610715743E-4 1.2591350246034984E-4 0.0 0.6272695908818477 2.518270049206997E-4 76-77 5.666107610715743E-4 1.2591350246034984E-4 0.0 0.6974348901278777 2.518270049206997E-4 78-79 5.666107610715743E-4 1.2591350246034984E-4 0.0 0.7841578149474437 2.518270049206997E-4 80-81 5.666107610715743E-4 1.2591350246034984E-4 0.0 0.9009425884794182 2.8330538053578715E-4 82-83 6.295675123017492E-4 1.2591350246034984E-4 0.0 1.0090078519660133 3.147837561508746E-4 84-85 6.295675123017492E-4 1.2591350246034984E-4 0.0 1.157208044361845 3.147837561508746E-4 86-87 6.295675123017492E-4 1.2591350246034984E-4 0.0 1.3826561705171017 3.4626213176596203E-4 88-89 6.295675123017492E-4 1.2591350246034984E-4 0.0 1.676097588000947 3.777405073810495E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 95 3.4774683E-5 30.017923 2 GCGTATC 70 0.008369389 27.158216 1 GTACATG 10160 0.0 26.242697 1 TACATGG 10305 0.0 25.320793 2 GTACCTG 1590 0.0 24.80963 1 TACCTGG 1510 0.0 24.551083 2 ACATGGG 10305 0.0 24.029387 3 GAGTACT 6595 0.0 23.171457 12-13 ATCACGC 130 2.9303358E-4 21.936172 3 CATGGGA 6820 0.0 21.32572 4 AGTACTT 6765 0.0 21.008284 12-13 GTACTTT 7335 0.0 20.83345 14-15 GTATAGG 510 0.0 19.56989 1 GTATCAA 17735 0.0 19.375208 1 CATGGGG 3265 0.0 19.215754 4 ACTTTTT 8460 0.0 18.426207 16-17 GTACACG 265 3.98466E-7 17.93467 1 ATGGGAT 2550 0.0 17.891342 5 TCACGCA 220 2.9026087E-5 17.28359 4 CAACGCA 20020 0.0 17.138983 5 >>END_MODULE