FastQCFastQC Report
Thu 26 May 2016
SRR936481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588392
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94250.5933673803443986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83790.5275146185576357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56720.35709069297755214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.320701690766511No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27380.1723755848682189No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24540.15449586751884925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23410.1473817546298395No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23380.14719288437614897No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18300.11521085475122009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.11206301718971136No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17310.1089781363794328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17100.035.556252
GTACCTG17900.035.0407521
GTACATG95900.024.2294061
TACATGG97950.023.1321532
ACATGGG95150.022.6133123
ACCTGGG26450.022.2674663
GAGTACT89250.021.84707812-13
GTACACG2657.894414E-1021.5173071
AGTACTT91400.021.02135812-13
CATGGGA61950.020.3939534
GTACTTT95800.020.30313714-15
GTATCAA191850.019.7400861
CGATTCG1250.006032552618.9997739
CATGGGG31900.018.611464
AGACCGT2854.2788088E-818.331966
TGCACCG2352.5297013E-618.19075
ACTTTTT109350.017.98219316-17
TACTTTT107450.017.88079614-15
GGTATCA153050.017.5415381
TCAACGC218300.017.2971464
Produced by FastQC (version 0.11.5)