FastQCFastQC Report
Thu 26 May 2016
SRR936480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636953
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77490.4733795044818025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.3725213857697808No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48380.2955491086182682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.22315851463053615No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33920.20721425722057996No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24620.1504013859897016No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22360.13659524738950965No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21110.1289591087832088No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19850.12126188106805753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16800.034.508042
GTACCTG17100.034.4605261
GTACATG100100.025.1141171
TACATGG103400.023.851552
ACATGGG102350.023.3076733
GAGTACT71050.023.24521312-13
GTACTTT77050.021.3405914-15
ACCTGGG26550.021.1203213
GTATCAA176250.020.8962761
CATGGGA65550.020.589324
CGGGAGT4950.020.1609234
CATGGGG34100.019.7892934
AGTACTT73750.019.52645512-13
GTCGGGA4150.019.4657252
ATGGGAT27400.018.7319415
ATCAACG197700.018.6044713
TATCAAC199400.018.5167882
ACTTTTT91200.018.49765216-17
TCAACGC198050.018.476184
CAACGCA199000.018.4601825
Produced by FastQC (version 0.11.5)