FastQCFastQC Report
Thu 26 May 2016
SRR936480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636953
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97570.5960464350534194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84120.5138815836496222No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59590.3640299996395742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.31919059374337566No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28660.17508138596526596No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25890.15815970281370326No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25140.15357801964992276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.14636950480557473No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19200.1172910889927811No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18970.11588603948922176No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.10934950484222822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16250.032.7387432
GTACCTG17900.030.522621
GTACACG3350.026.9454731
GTACATG94250.023.489921
GAGTACT94350.022.8020612-13
ACATGGG95150.022.3108713
TACATGG99550.022.0443922
GTACTTT100350.021.2257414-15
GTATAGG5050.020.6970441
AGTACTT96200.020.53695512-13
CATGGGA63200.019.762564
GTATCAA200600.019.4204771
ACCTGGG26100.019.2878193
CATGGGG31400.019.05664
ACTTTTT115300.018.78192116-17
TAACGCA2051.592878E-518.5327824
ATGGGAG24850.018.3463345
GGTATCA160600.017.867691
TATAGGG3208.276402E-917.8126542
TACTTTT112700.017.50914214-15
Produced by FastQC (version 0.11.5)