##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636953 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87860494467465 31.0 31.0 34.0 30.0 34.0 2 32.03082739699918 31.0 31.0 34.0 30.0 34.0 3 32.10943747315897 33.0 31.0 34.0 30.0 34.0 4 35.812255452661134 37.0 35.0 37.0 35.0 37.0 5 35.65269131123496 37.0 35.0 37.0 33.0 37.0 6 35.747220598270076 37.0 35.0 37.0 33.0 37.0 7 35.69576585277647 37.0 35.0 37.0 33.0 37.0 8 35.671719957750774 37.0 35.0 37.0 33.0 37.0 9 37.29393513436244 39.0 37.0 39.0 34.0 39.0 10-11 37.191329256246206 39.0 37.0 39.0 33.0 39.0 12-13 37.088172659813694 39.0 37.0 39.0 33.0 39.0 14-15 38.187977296843584 40.0 37.5 41.0 33.0 41.0 16-17 38.09763078109145 40.0 37.0 41.0 33.0 41.0 18-19 38.286932489814916 40.0 38.0 41.0 33.5 41.0 20-21 38.32068422245477 40.0 38.0 41.0 34.0 41.0 22-23 38.23382711660017 40.0 38.0 41.0 33.5 41.0 24-25 38.102208798908705 40.0 37.0 41.0 33.0 41.0 26-27 37.89561948327166 40.0 37.0 41.0 33.0 41.0 28-29 37.80595899821192 40.0 37.0 41.0 33.0 41.0 30-31 37.729880760168435 40.0 37.0 41.0 33.0 41.0 32-33 37.52903443165442 40.0 36.0 41.0 32.5 41.0 34-35 37.33913863134738 39.0 36.0 41.0 32.0 41.0 36-37 37.118055619190045 39.0 36.0 41.0 31.0 41.0 38-39 36.937754779764596 39.0 35.5 41.0 31.0 41.0 40-41 36.73711126709197 39.0 35.0 41.0 31.0 41.0 42-43 36.43834795501154 39.0 35.0 41.0 30.0 41.0 44-45 36.124500214728215 39.0 35.0 40.0 30.0 41.0 46-47 35.68589812902386 39.0 35.0 40.0 28.5 41.0 48-49 35.24670622797356 38.0 35.0 40.0 26.5 41.0 50-51 34.946260216389845 38.0 34.0 40.0 26.0 41.0 52-53 34.59899093010001 38.0 34.0 40.0 24.0 41.0 54-55 34.41510293820287 38.0 34.0 40.0 24.0 41.0 56-57 34.12380654789722 38.0 33.5 40.0 23.0 41.0 58-59 33.77032083389077 37.5 33.0 40.0 22.5 41.0 60-61 33.359663044693406 37.0 32.5 40.0 22.0 41.0 62-63 33.047932042031746 36.5 32.0 39.0 21.0 40.5 64-65 32.63733931273531 36.0 31.5 39.0 19.5 40.0 66-67 32.22198896364159 35.5 31.0 39.0 18.5 40.0 68-69 31.873496673392577 35.0 31.0 38.0 18.0 40.0 70-71 31.51454592770837 35.0 31.0 38.0 15.5 40.0 72-73 31.056271621726466 35.0 30.0 37.0 12.5 39.0 74-75 30.562138314294913 34.0 30.0 36.5 8.0 39.0 76-77 29.061097050434558 32.5 28.0 35.0 7.0 37.5 78-79 29.813016928402952 34.0 29.0 36.0 7.0 37.5 80-81 29.581948595958465 34.0 29.0 35.0 3.5 37.0 82-83 29.28223382100769 34.0 29.0 35.0 2.0 37.0 84-85 28.897271332774977 34.0 29.0 35.0 2.0 36.0 86-87 28.522691854928027 34.0 29.0 35.0 2.0 36.0 88-89 28.048850211337772 33.0 27.5 35.0 2.0 35.5 90-91 27.583199090016635 33.0 26.5 35.0 2.0 35.0 92-93 26.99059013911823 33.0 25.5 35.0 2.0 35.0 94-95 26.476241223785898 32.0 25.0 34.0 2.0 35.0 96-97 25.77183920369125 32.0 23.5 34.0 2.0 35.0 98-99 24.910537443652935 32.0 19.0 34.0 2.0 35.0 100-101 21.71602788839997 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42786387193674 1101 2 0.5165745448481687 1101 3 0.47064345481501135 1101 4 0.3831547359637355 1101 5 0.37206733928147173 1101 6 0.36460103324860427 1101 7 0.3999107618069573 1101 8 0.288755882573831 1101 9 0.3172983869324071 1101 10-11 0.5143507344102503 1101 12-13 0.3967276871190961 1101 14-15 0.6024009670366794 1101 16-17 0.7187648330985255 1101 18-19 0.6316000681928244 1101 20-21 0.5760762316718981 1101 22-23 0.5547147651362678 1101 24-25 0.4745492629178827 1101 26-27 0.5645662979348813 1101 28-29 0.6086632794434337 1101 30-31 0.667269645672441 1101 32-33 0.6380152116015765 1101 34-35 0.6506236690034228 1101 36-37 0.5925721543602194 1101 38-39 0.6106518007413513 1101 40-41 0.7370133649386972 1101 42-43 0.8259350808387893 1101 44-45 0.8483332385936606 1101 46-47 0.9256153000953162 1101 48-49 0.9100450214041587 1101 50-51 0.7041585585852985 1101 52-53 0.6999623724503508 1101 54-55 0.645678730521233 1101 56-57 0.6296112004196743 1101 58-59 0.6075452267447687 1101 60-61 0.7434466411703866 1101 62-63 0.8294868094954992 1101 64-65 0.8959386417586757 1101 66-67 0.6940420648781824 1101 68-69 0.6144039903040692 1101 70-71 0.6039456758631445 1101 72-73 0.681863395529394 1101 74-75 0.8349753109145688 1101 76-77 0.6963010731907495 1101 78-79 0.7892388164020154 1101 80-81 0.9463514253567382 1101 82-83 1.0828695047318924 1101 84-85 0.9092536664447906 1101 86-87 0.7909199935653319 1101 88-89 0.8557495568957059 1101 90-91 0.9865553805086016 1101 92-93 0.8509624529312951 1101 94-95 0.6994854712660725 1101 96-97 1.177866262094195 1101 98-99 1.1875112713860965 1101 100-101 1.3674108062566326 1102 1 0.3414089030141021 1102 2 0.3821194469164624 1102 3 0.37871159003494625 1102 4 0.31687125695765417 1102 5 0.2937339521943585 1102 6 0.30474543126002374 1102 7 0.29592069550550804 1102 8 0.3724680264742304 1102 9 0.44082549496368273 1102 10-11 0.4397542333205777 1102 12-13 0.3983921345225596 1102 14-15 0.5145434852016137 1102 16-17 0.5563716687363538 1102 18-19 0.4876499025543879 1102 20-21 0.3601030655589028 1102 22-23 0.4139981197407394 1102 24-25 0.410711427321516 1102 26-27 0.3291950052179047 1102 28-29 0.427131388861568 1102 30-31 0.6174871181082651 1102 32-33 0.5307856353503055 1102 34-35 0.49756477175453995 1102 36-37 0.5915488359704355 1102 38-39 0.4720529774883886 1102 40-41 0.4550027585499805 1102 42-43 0.5384134833378766 1102 44-45 0.33227575583710234 1102 46-47 0.2386530490713099 1102 48-49 0.3879169706851613 1102 50-51 0.11283741353764043 1102 52-53 0.3908306035055915 1102 54-55 0.2589945401222735 1102 56-57 0.21466780315005707 1102 58-59 0.397879222017508 1102 60-61 0.4636259637806077 1102 62-63 0.538718560471132 1102 64-65 0.3412719689487176 1102 66-67 0.34335048410238045 1102 68-69 0.4619058217721488 1102 70-71 0.337615891815112 1102 72-73 0.39657941430942856 1102 74-75 0.4526709785134777 1102 76-77 0.28556175905826464 1102 78-79 0.3472122681779588 1102 80-81 0.34047621398919503 1102 82-83 0.3601235927068629 1102 84-85 0.4391957505863502 1102 86-87 0.4600545730648662 1102 88-89 0.42539625633681766 1102 90-91 0.43762730767376823 1102 92-93 0.4696600141990821 1102 94-95 0.34126644150644836 1102 96-97 0.402710414527629 1102 98-99 0.2772937528756003 1102 100-101 1.0373270425091015 1103 1 0.31900013906838254 1103 2 0.2889190839539353 1103 3 0.19843759504828284 1103 4 0.2521881364534053 1103 5 0.372374963483054 1103 6 0.2878801897891208 1103 7 0.21880593468181786 1103 8 0.163200487631336 1103 9 0.3955447031212955 1103 10-11 0.32300215688984935 1103 12-13 0.36213238685799354 1103 14-15 0.4507108408249252 1103 16-17 0.3895418891213609 1103 18-19 0.3843937567018614 1103 20-21 0.14448150961782602 1103 22-23 0.15817994171526806 1103 24-25 0.3454902948367149 1103 26-27 0.34851563254509443 1103 28-29 0.2964066458731054 1103 30-31 0.4590304215539831 1103 32-33 0.3394819601809118 1103 34-35 0.5151131754495282 1103 36-37 0.3716853293491553 1103 38-39 0.31603906392492576 1103 40-41 0.44436811301208934 1103 42-43 0.48898765250483933 1103 44-45 0.6272623359582994 1103 46-47 0.6500483861849204 1103 48-49 0.6266743455343615 1103 50-51 0.6517754874025954 1103 52-53 0.616594858650167 1103 54-55 0.5976613481728137 1103 56-57 0.6008526289523886 1103 58-59 0.6718529141877383 1103 60-61 0.6974995109744242 1103 62-63 0.7097333605809908 1103 64-65 0.6916246753488906 1103 66-67 0.34235442314970754 1103 68-69 0.5632663228273174 1103 70-71 0.45462958634946915 1103 72-73 0.44026659554385006 1103 74-75 0.45659438090472193 1103 76-77 0.4487236702436981 1103 78-79 0.3592267101926119 1103 80-81 0.4869171647841313 1103 82-83 0.6046378695805181 1103 84-85 0.8899880797001813 1103 86-87 0.8542587325319673 1103 88-89 0.6609120872741592 1103 90-91 0.8513619973651423 1103 92-93 0.6917872562053908 1103 94-95 0.7916146282583156 1103 96-97 1.0695888672781813 1103 98-99 1.0793879145329868 1103 100-101 1.606269931537831 1104 1 0.22799071745270894 1104 2 0.23906330564513922 1104 3 0.1769589808787444 1104 4 0.20719454992364206 1104 5 0.20148039832935893 1104 6 0.17117456481760485 1104 7 0.14599839738927756 1104 8 0.17020321579786213 1104 9 0.2768238811553232 1104 10-11 0.247835146218101 1104 12-13 0.20127880931360664 1104 14-15 0.29858869840030877 1104 16-17 0.40438728216616227 1104 18-19 0.188372712706105 1104 20-21 0.26232902529338986 1104 22-23 0.2863760962375821 1104 24-25 0.3258461936015564 1104 26-27 0.33015341146593613 1104 28-29 0.43821677459828834 1104 30-31 0.49369083318807583 1104 32-33 0.45718053359072286 1104 34-35 0.41677058544932777 1104 36-37 0.2905980670870534 1104 38-39 0.3872367225199511 1104 40-41 0.43212636099526947 1104 42-43 0.5598914960291879 1104 44-45 0.5080401564202006 1104 46-47 0.4954959617629484 1104 48-49 0.6737270792353627 1104 50-51 0.6599201062731694 1104 52-53 0.7710431048753392 1104 54-55 0.7370167781357608 1104 56-57 0.6768620180123719 1104 58-59 0.6166287031748041 1104 60-61 0.7102597122987859 1104 62-63 0.6413257243191666 1104 64-65 0.7306841741215422 1104 66-67 0.6536445724500837 1104 68-69 0.5227328653482957 1104 70-71 0.671032931771812 1104 72-73 0.8234234227968642 1104 74-75 0.5071199924632914 1104 76-77 0.4985778207681726 1104 78-79 0.5600432068100751 1104 80-81 0.6352278502115567 1104 82-83 0.5770973865843523 1104 84-85 0.9296490447809518 1104 86-87 0.7406222949054069 1104 88-89 0.7304803337597754 1104 90-91 0.9018176141977357 1104 92-93 0.8977874320060977 1104 94-95 0.8551527477116387 1104 96-97 1.107907724182919 1104 98-99 1.2828723496592715 1104 100-101 1.692382977903641 1105 1 0.06351097720601118 1105 2 0.179937934204915 1105 3 0.14239621147522286 1105 4 0.1474396510208109 1105 5 0.29907237019956057 1105 6 0.15075429152280861 1105 7 0.13262692978008062 1105 8 0.3192057095634482 1105 9 0.20216953167211216 1105 10-11 0.20703320811034587 1105 12-13 0.20697783965113103 1105 14-15 0.38413976886326395 1105 16-17 0.3246978541194423 1105 18-19 0.34965289388829746 1105 20-21 0.23158944654044689 1105 22-23 0.36431045497162273 1105 24-25 0.3453082918641357 1105 26-27 0.2916637980985044 1105 28-29 0.3452515442939301 1105 30-31 0.22906886918411828 1105 32-33 0.0475726892601287 1105 34-35 0.25169593224099174 1105 36-37 0.2454875745569396 1105 38-39 0.061653867576772825 1105 40-41 0.15423924534935907 1105 42-43 0.19263981893759308 1105 44-45 0.3288684624093463 1105 46-47 0.16697924257859853 1105 48-49 0.34363901385884077 1105 50-51 0.4269207711758938 1105 52-53 0.3802307443793822 1105 54-55 0.20935541750439057 1105 56-57 0.27421663330452617 1105 58-59 0.3247491509875502 1105 60-61 0.3312955911799875 1105 62-63 0.3541501306614592 1105 64-65 0.3439042127984919 1105 66-67 0.1930645931731405 1105 68-69 0.4296086365669822 1105 70-71 0.26726409729911893 1105 72-73 0.40709884376244787 1105 74-75 0.2331867276996391 1105 76-77 0.209226078099217 1105 78-79 0.3002454011412077 1105 80-81 0.3428783396073456 1105 82-83 0.5556012109161408 1105 84-85 0.567975437463069 1105 86-87 0.7043223340083813 1105 88-89 0.600279409894938 1105 90-91 0.39901212791344776 1105 92-93 0.4502264990730218 1105 94-95 0.49137583398619 1105 96-97 0.6734978291523426 1105 98-99 0.5638858013868777 1105 100-101 1.2130543014252204 1106 1 0.24488899002804132 1106 2 0.2599078374252315 1106 3 0.17012829551911324 1106 4 0.2646032954332469 1106 5 0.25112880381740155 1106 6 0.1245302803579662 1106 7 0.12989392946603573 1106 8 0.17904080968123282 1106 9 0.31647225727039796 1106 10-11 0.3225500814947466 1106 12-13 0.3343013031379414 1106 14-15 0.3548820051804711 1106 16-17 0.32097097017817333 1106 18-19 0.3452680920433977 1106 20-21 0.306449436867517 1106 22-23 0.23019293811404395 1106 24-25 0.24050962336637127 1106 26-27 0.3303226732349529 1106 28-29 0.04373955480912173 1106 30-31 0.2374692698995986 1106 32-33 0.2692595821413164 1106 34-35 0.38869736449700554 1106 36-37 0.19011862670097912 1106 38-39 0.45246895994758773 1106 40-41 0.46716832029019884 1106 42-43 0.4464387468655815 1106 44-45 0.43577419294256003 1106 46-47 0.38528635621263163 1106 48-49 0.26137897423284784 1106 50-51 0.31641050571626295 1106 52-53 0.12604231180500847 1106 54-55 0.23573501493481075 1106 56-57 0.19066878495837614 1106 58-59 0.23167640488636465 1106 60-61 0.1459321625042591 1106 62-63 0.2325339522769596 1106 64-65 0.40623286129641656 1106 66-67 0.21686659689427046 1106 68-69 0.0805693548077997 1106 70-71 0.29556516714333725 1106 72-73 0.5049821878395022 1106 74-75 0.401907097139766 1106 76-77 0.3313009305437902 1106 78-79 0.2409028950453198 1106 80-81 0.5068899787535699 1106 82-83 0.5069000148781662 1106 84-85 0.8164889238817885 1106 86-87 0.9436916239385376 1106 88-89 0.8027580464274564 1106 90-91 0.7026045896331183 1106 92-93 0.9482935283670777 1106 94-95 0.8882226788907559 1106 96-97 1.0837062285110264 1106 98-99 1.0208433820312273 1106 100-101 1.4457608700401998 1107 1 0.041288840747117916 1107 2 0.11473281502293275 1107 3 0.030856439647003242 1107 4 0.1780863973608291 1107 5 0.08020688573361667 1107 6 0.06638048331635815 1107 7 0.018114756864370918 1107 8 0.05238569806258653 1107 9 0.052829883974617076 1107 10-11 0.10020447447881509 1107 12-13 0.08735448412887337 1107 14-15 0.10844588407327649 1107 16-17 0.1528883698724357 1107 18-19 0.12385549080972424 1107 20-21 0.12601763298634694 1107 22-23 0.07684099207734363 1107 24-25 0.10205224259637902 1107 26-27 0.03706371621012039 1107 28-29 0.16811159619868477 1107 30-31 0.15890764308971939 1107 32-33 0.14469585970901022 1107 34-35 0.14111555970146128 1107 36-37 0.1530991246788389 1107 38-39 0.16046287221184485 1107 40-41 0.11471581892031679 1107 42-43 0.1564175400336083 1107 44-45 0.0012312370854203891 1107 46-47 -0.10845132929635071 1107 48-49 -0.057061317799650624 1107 50-51 0.03547095026727476 1107 52-53 0.17118116541691109 1107 54-55 0.058457820440871444 1107 56-57 -0.14345887326145856 1107 58-59 -0.11454239868526628 1107 60-61 0.01159880275550762 1107 62-63 0.09208711870554254 1107 64-65 0.06550930938573885 1107 66-67 0.02626875523636585 1107 68-69 -0.059746925685907115 1107 70-71 -0.05126085216971177 1107 72-73 0.11212698017713407 1107 74-75 0.14826278671401028 1107 76-77 0.011994179239447789 1107 78-79 0.050034803436680875 1107 80-81 0.15968008936640032 1107 82-83 0.13971381130748028 1107 84-85 0.32760828698596356 1107 86-87 0.25644566058551277 1107 88-89 0.15598912503632434 1107 90-91 0.29090401980534253 1107 92-93 0.3916333233157765 1107 94-95 0.3271629536516478 1107 96-97 0.6326394185238335 1107 98-99 0.4458303944552746 1107 100-101 1.1838204279817255 1108 1 0.05488876232296036 1108 2 0.08431616646321771 1108 3 0.023861934391270267 1108 4 0.07786753062362095 1108 5 0.0981275472993417 1108 6 0.05310126534806159 1108 7 0.10379685671840377 1108 8 0.13642890815746966 1108 9 0.16322570027477923 1108 10-11 0.054972801494081125 1108 12-13 0.038789784365725666 1108 14-15 0.07488755290435734 1108 16-17 0.14117814040428556 1108 18-19 0.1567355271112163 1108 20-21 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2316 76-77 -1.169349831292461 2316 78-79 -1.3119988391633406 2316 80-81 -1.1718828940545407 2316 82-83 -1.5747728577791307 2316 84-85 -1.4182128944810088 2316 86-87 -1.6312760641904518 2316 88-89 -1.7400195528872473 2316 90-91 -1.60963063777157 2316 92-93 -1.489624320303271 2316 94-95 -1.8223911486294533 2316 96-97 -1.786268087887418 2316 98-99 -2.4284575202881378 2316 100-101 -3.4692408115344158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 18.0 5 54.0 6 152.0 7 342.0 8 692.0 9 1361.0 10 2289.0 11 3823.0 12 5537.0 13 7529.0 14 9613.0 15 12766.0 16 16182.0 17 21088.0 18 18228.0 19 11601.0 20 10089.0 21 10394.0 22 10968.0 23 12127.0 24 14263.0 25 17141.0 26 21035.0 27 26022.0 28 32200.0 29 39864.0 30 48803.0 31 60053.0 32 74510.0 33 94677.0 34 123842.0 35 166616.0 36 233253.0 37 312491.0 38 204368.0 39 12951.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.765779857996556 19.677314888364762 15.132960197484998 21.423945056153688 2 15.486385031599632 21.48218885483721 39.77172749534437 23.25969861821879 3 19.74413646055437 26.15025568025611 30.2569632394719 23.84864461971762 4 12.553078799452397 17.511254141077966 37.87671362586464 32.058953433605 5 12.86164263555757 38.36316807440379 34.03536916597532 14.739820124063325 6 29.94624769312253 37.11395501275846 17.950912457474345 14.98888483664467 7 27.61808066572467 33.785576006152894 20.913856414936777 17.682486913185656 8 26.450728884702247 33.801825709107106 19.92635097036995 19.8210944358207 9 26.689587300307338 16.936039092142536 20.931144632741443 35.44322897480868 10-11 24.681160668632515 27.12997868600992 27.11351517117474 21.07534547418283 12-13 26.577665046872617 24.706425692138943 27.7071224204482 21.008786840540242 14-15 23.081944139422664 25.7593616911446 27.3898019518611 23.76889221757164 16-17 21.05570532568742 29.0824782385322 27.97361927923404 21.88819715654634 18-19 20.84474191258493 27.276248284894976 31.166191996461663 20.71281780605843 20-21 22.236710522537912 25.863296013996738 31.068424078150077 20.831569385315277 22-23 21.610309401158556 26.05753679474511 31.033185141291515 21.298968662804814 24-25 21.713054681472226 26.178607449328112 31.361468533305477 20.74686933589419 26-27 21.492445586966774 26.168197179588056 31.443304637234228 20.896052596210943 28-29 20.882297959063983 26.67179222137509 31.531936054581355 20.91397376497957 30-31 21.23587138331238 26.23121020742538 31.32896661180774 21.203951797454497 32-33 21.072469231100545 26.40677354132766 31.180912972835763 21.339844254736033 34-35 21.827822984629673 26.16716149842387 31.029647142193877 20.975368374752584 36-37 21.785448327640797 25.92384322201377 31.136071655693954 21.15463679465148 38-39 21.535297554848746 26.295112171630674 31.180222043229 20.98936823029158 40-41 21.55894661509519 26.346718274793112 31.089619942924397 21.0047151671873 42-43 21.363652469704487 26.63020900789064 30.449832485783894 21.556306036620978 44-45 21.59404446309724 26.547309125219424 30.21472200560745 21.643924406075886 46-47 22.020960328953336 26.969738528891696 29.480078646286888 21.529222495868076 48-49 22.08917640252751 27.04966228279299 29.21188933175275 21.64927198292675 50-51 21.83771367831382 27.367745857011734 29.23148350364473 21.56305696102972 52-53 22.19877076344985 27.612585837938948 28.659135184865033 21.529508213746166 54-55 21.72507186424919 27.27087514787495 28.945798579728464 22.058254408147395 56-57 22.14528855268339 27.573103872367632 28.44965078950013 21.831956785448845 58-59 22.523885525457118 27.065709942097914 28.492364453156505 21.918040079288456 60-61 22.048225308877704 27.312298101427857 29.11549255155803 21.523984038136415 62-63 22.153692378799168 27.208445483491282 29.030463602863342 21.607398534846205 64-65 21.91979099394574 27.53175751925064 28.84879871008658 21.699652776717038 66-67 21.895310641516165 27.55301270252224 28.481678079367395 22.069998576594198 68-69 21.94446243187008 27.454418166512518 28.676388836284154 21.92473056533325 70-71 22.06200240996608 27.4843344568642 28.539221510703577 21.91444162246614 72-73 22.076908157078474 27.263436171788737 28.600554994723424 22.059100676409365 74-75 21.78121498764144 28.073878901987996 28.242457490338573 21.902448620031986 76-77 22.015271400609436 27.514606736081753 28.457232991796772 22.012888871512036 78-79 21.993696938002174 27.54111851399983 28.455908654781652 22.009275893216344 80-81 22.154942821164692 27.813102772823722 28.10515260214565 21.926801803865935 82-83 22.072710853900098 27.7406638471756 28.25734312049475 21.92928217842955 84-85 21.866056585851123 27.75149716972975 28.112938277896575 22.269507966522546 86-87 22.031708734916112 27.632041578575816 28.108457668585345 22.227792017922727 88-89 22.13438203757857 27.571357418085256 27.838575326726982 22.455685217609187 90-91 21.92043373445377 27.395316672661217 28.21220522934577 22.472044363539244 92-93 22.063982567429914 27.627458253308784 28.146652840217122 22.16190633904418 94-95 21.716006668019173 27.442272015095924 28.18991877629138 22.651802540593522 96-97 21.58359364638537 27.2907599511298 28.354615746153673 22.771030656331153 98-99 21.601878846776806 27.532867594477146 27.81102737590993 23.054226182836118 100-101 21.469559726822684 27.549576499367056 27.972557574631406 23.008306199178854 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 690.0 1 537.5 2 811.5 3 1049.0 4 706.0 5 581.0 6 825.5 7 1530.0 8 2338.0 9 2735.0 10 2568.0 11 2190.0 12 2133.5 13 2305.5 14 2516.0 15 2711.5 16 2862.0 17 3082.5 18 3292.0 19 3478.5 20 3888.5 21 4451.5 22 5000.5 23 5756.0 24 6694.5 25 7926.0 26 9858.0 27 12429.0 28 14709.5 29 17355.0 30 20930.0 31 24732.0 32 29312.5 33 33171.5 34 36471.0 35 39690.5 36 43763.5 37 48106.0 38 50007.0 39 50580.0 40 51056.0 41 52046.5 42 53003.0 43 54519.5 44 56193.5 45 57682.0 46 58206.0 47 61988.5 48 75719.5 49 74688.5 50 60213.0 51 53269.0 52 48521.5 53 46238.0 54 44271.0 55 41052.5 56 37556.5 57 33981.0 58 31427.5 59 28612.5 60 24535.0 61 20573.5 62 17017.0 63 12897.0 64 9064.0 65 6824.0 66 5227.0 67 4006.5 68 2974.0 69 2146.5 70 1646.5 71 1351.5 72 1137.0 73 1002.5 74 846.5 75 691.5 76 500.5 77 279.0 78 178.5 79 122.5 80 76.0 81 61.0 82 48.5 83 31.5 84 20.5 85 10.0 86 2.5 87 1.0 88 2.0 89 2.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022786237601201745 2 0.013745049491341536 3 0.008735742565608176 4 0.0 5 0.002871188115969121 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010079702960317126 14-15 1.5272277212601706E-4 16-17 0.0 18-19 0.0016494059389609843 20-21 0.0 22-23 3.054455442520341E-5 24-25 0.0 26-27 0.001771584156661798 28-29 0.0034820792044731887 30-31 0.0016494059389609843 32-33 0.006933613854521175 34-35 0.002015940592063425 36-37 8.552475239056955E-4 38-39 0.001191237622582933 40-41 9.163366327561024E-5 42-43 0.004703861381481325 44-45 0.0013745049491341534 46-47 2.1381188097642387E-4 48-49 3.970792075276443E-4 50-51 3.054455442520341E-5 52-53 1.2217821770081364E-4 54-55 7.636138606300852E-4 56-57 0.011576386127152094 58-59 0.02425237621361151 60-61 0.01844891087282286 62-63 0.012004009889104942 64-65 0.015516633648003333 66-67 0.002077029700913832 68-69 4.2762376195284774E-4 70-71 3.054455442520341E-5 72-73 3.054455442520341E-5 74-75 0.0018937623743626114 76-77 0.0022602970274650525 78-79 0.007911039596127684 80-81 0.015027920777200079 82-83 0.015333366321452113 84-85 0.02739846531940746 86-87 0.009071732664285412 88-89 0.039585742535063626 90-91 0.045480841539127875 92-93 0.029078415812793645 94-95 0.02962821779244731 96-97 0.047771683121018135 98-99 0.051498118760892955 100-101 0.057790296972484854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636953.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.51416307271382 #Duplication Level Percentage of deduplicated Percentage of total 1 79.47597146639424 45.70993983280549 2 12.441646838069916 14.31141810275735 3 3.6555382893486597 6.307356758764445 4 1.4429642745567983 3.319635303798396 5 0.7549814362304287 2.171106272011428 6 0.44959699394396674 1.5514916896017141 7 0.29611066635853184 1.1921390006760189 8 0.21583584969566194 0.9930894605067242 9 0.154376202736386 0.7990936288854151 >10 0.9733977575677911 10.729602614266387 >50 0.08728504912069605 3.412096281754434 >100 0.04760907524378373 5.026350997616906 >500 0.0025560549453706766 0.9847789566696117 >1k 0.0017040366302471177 1.6939153615563503 >5k 4.260091575617794E-4 1.797985738329484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9757 0.5960464350534194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8412 0.5138815836496222 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5959 0.3640299996395742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.31919059374337566 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2866 0.17508138596526596 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2589 0.15815970281370326 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2514 0.15357801964992276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2396 0.14636950480557473 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1920 0.1172910889927811 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1897 0.11588603948922176 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.10934950484222822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.054455442520341E-5 14-15 0.0 0.0 0.0 0.0 6.108910885040682E-5 16-17 3.054455442520341E-5 0.0 0.0 0.0 6.108910885040682E-5 18-19 9.163366327561023E-5 0.0 0.0 0.0 6.108910885040682E-5 20-21 1.8326732655122048E-4 0.0 0.0 0.0 6.108910885040682E-5 22-23 1.8326732655122048E-4 0.0 0.0 2.749009898268307E-4 6.108910885040682E-5 24-25 2.443564354016273E-4 0.0 0.0 5.498019796536614E-4 6.108910885040682E-5 26-27 2.443564354016273E-4 0.0 0.0 8.552475239056955E-4 6.108910885040682E-5 28-29 2.749009898268307E-4 0.0 0.0 0.0014661386124097638 6.108910885040682E-5 30-31 3.054455442520341E-4 0.0 0.0 0.00250465346286668 6.108910885040682E-5 32-33 3.054455442520341E-4 0.0 0.0 0.004734405935906528 1.5272277212601706E-4 34-35 3.054455442520341E-4 0.0 0.0 0.006780891082395157 1.8326732655122048E-4 36-37 3.054455442520341E-4 0.0 0.0 0.00974371286163989 1.8326732655122048E-4 38-39 3.6653465310244095E-4 0.0 0.0 0.014539207906396824 1.8326732655122048E-4 40-41 3.6653465310244095E-4 0.0 0.0 0.01994559403965783 1.8326732655122048E-4 42-43 3.970792075276443E-4 0.0 0.0 0.02425237621361151 2.1381188097642387E-4 44-45 4.2762376195284774E-4 0.0 0.0 0.026390495023375746 2.443564354016273E-4 46-47 4.887128708032546E-4 0.0 0.0 0.02990311878227414 2.443564354016273E-4 48-49 5.498019796536614E-4 0.0 0.0 0.03616475243944084 3.054455442520341E-4 50-51 5.498019796536614E-4 0.0 0.0 0.042487475205457946 3.054455442520341E-4 52-53 5.498019796536614E-4 0.0 0.0 0.05232282173037345 3.6653465310244095E-4 54-55 5.498019796536614E-4 3.054455442520341E-5 0.0 0.06023386132650113 3.6653465310244095E-4 56-57 5.498019796536614E-4 6.108910885040682E-5 0.0 0.0788660395258752 3.6653465310244095E-4 58-59 5.498019796536614E-4 6.108910885040682E-5 0.0 0.11267886127457538 3.6653465310244095E-4 60-61 5.498019796536614E-4 6.108910885040682E-5 0.0 0.13919153451565194 3.6653465310244095E-4 62-63 5.498019796536614E-4 6.108910885040682E-5 0.0 0.15531905925215936 3.6653465310244095E-4 64-65 5.498019796536614E-4 6.108910885040682E-5 0.0 0.18668831664684327 3.6653465310244095E-4 66-67 5.498019796536614E-4 6.108910885040682E-5 0.0 0.25318381163051107 3.6653465310244095E-4 68-69 5.498019796536614E-4 6.108910885040682E-5 0.0 0.34075504916756927 3.6653465310244095E-4 70-71 5.498019796536614E-4 6.108910885040682E-5 0.0 0.42001816790097213 3.6653465310244095E-4 72-73 5.498019796536614E-4 6.108910885040682E-5 0.0 0.5083224747442352 3.6653465310244095E-4 74-75 5.498019796536614E-4 6.108910885040682E-5 0.0 0.6113492568204463 3.6653465310244095E-4 76-77 5.498019796536614E-4 9.163366327561023E-5 0.0 0.6824569795223199 3.6653465310244095E-4 78-79 5.498019796536614E-4 1.2217821770081364E-4 0.0 0.7649578210247943 3.6653465310244095E-4 80-81 6.108910885040682E-4 1.2217821770081364E-4 0.0 0.879347177347181 3.6653465310244095E-4 82-83 6.719801973544751E-4 1.2217821770081364E-4 0.0 0.987016731696023 3.6653465310244095E-4 84-85 6.719801973544751E-4 1.2217821770081364E-4 0.0 1.135341087984811 3.6653465310244095E-4 86-87 6.719801973544751E-4 1.2217821770081364E-4 0.0 1.3717559392358853 3.970792075276443E-4 88-89 6.719801973544751E-4 1.2217821770081364E-4 0.0 1.6586609389518208 4.2762376195284774E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1625 0.0 32.738743 2 GTACCTG 1790 0.0 30.52262 1 GTACACG 335 0.0 26.945473 1 GTACATG 9425 0.0 23.48992 1 GAGTACT 9435 0.0 22.80206 12-13 ACATGGG 9515 0.0 22.310871 3 TACATGG 9955 0.0 22.044392 2 GTACTTT 10035 0.0 21.22574 14-15 GTATAGG 505 0.0 20.697044 1 AGTACTT 9620 0.0 20.536955 12-13 CATGGGA 6320 0.0 19.76256 4 GTATCAA 20060 0.0 19.420477 1 ACCTGGG 2610 0.0 19.287819 3 CATGGGG 3140 0.0 19.0566 4 ACTTTTT 11530 0.0 18.781921 16-17 TAACGCA 205 1.592878E-5 18.532782 4 ATGGGAG 2485 0.0 18.346334 5 GGTATCA 16060 0.0 17.86769 1 TATAGGG 320 8.276402E-9 17.812654 2 TACTTTT 11270 0.0 17.509142 14-15 >>END_MODULE