FastQCFastQC Report
Thu 26 May 2016
SRR936479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166447
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59580.5107818872181934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.412191895559764No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32060.2748517506581954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.2516188047978177No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18610.15954432563159748No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15000.12859564129360357No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.11342135562095834No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12930.11084944279508627No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12760.10939202552709211No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12540.10750595612145257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10550.034.2162932
GTACCTG12900.027.9842931
GTATCAA121000.023.5142941
GTACATG75150.023.386391
TACATGG76250.022.7365652
GAGTACT56150.022.5472612-13
TATAACG1503.0344361E-522.1655542
TATAGGG2154.423964E-822.0919172
ATTCGCG1406.548362E-1122.06429580-81
ACATGGG75550.021.6926363
TAAGGTA2901.05501385E-1021.2956894
GTACTTT60350.021.05727414-15
CTAGTAC2509.480573E-920.9016423
CGTTTAG804.982504E-520.78644890-91
ATCAACG137750.020.5533393
TAACGCA1856.5453532E-620.5430364
TCAACGC137950.020.5244224
CAACGCA139250.020.3669265
TATCAAC139450.020.3342272
AACGCAG141150.020.1946036
Produced by FastQC (version 0.11.5)