##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936479_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1166447 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.560288637203406 31.0 31.0 34.0 30.0 34.0 2 31.61257305304056 33.0 31.0 34.0 30.0 34.0 3 31.62373172548774 33.0 31.0 34.0 28.0 34.0 4 35.269827947604995 37.0 35.0 37.0 33.0 37.0 5 35.285874111725605 37.0 35.0 37.0 33.0 37.0 6 35.29448144664953 37.0 35.0 37.0 33.0 37.0 7 35.215608596018505 37.0 35.0 37.0 32.0 37.0 8 35.17392389024105 37.0 35.0 37.0 32.0 37.0 9 36.6997754720103 39.0 37.0 39.0 32.0 39.0 10-11 36.5754942144821 39.0 37.0 39.0 32.0 39.0 12-13 36.48619397195072 39.0 37.0 39.0 32.0 39.0 14-15 37.57813428299785 40.0 37.0 41.0 32.0 41.0 16-17 37.50006858434202 40.0 37.0 41.0 32.0 41.0 18-19 37.50373270281462 40.0 37.0 41.0 32.0 41.0 20-21 37.37855256175377 40.0 37.0 41.0 32.0 41.0 22-23 37.468863994677854 40.0 37.0 41.0 32.0 41.0 24-25 37.498463710738676 40.0 37.0 41.0 32.0 41.0 26-27 37.4003628111693 40.0 37.0 41.0 32.0 41.0 28-29 37.260186703725076 39.5 37.0 41.0 31.0 41.0 30-31 37.034695532673155 39.0 36.0 41.0 31.0 41.0 32-33 36.80113155591296 39.0 35.5 41.0 30.5 41.0 34-35 36.5892698082296 39.0 35.0 41.0 30.0 41.0 36-37 36.37050633247803 39.0 35.0 40.5 30.0 41.0 38-39 36.154278334120626 39.0 35.0 40.0 30.0 41.0 40-41 35.93215465426204 38.5 35.0 40.0 29.0 41.0 42-43 35.66558660616384 38.0 35.0 40.0 28.0 41.0 44-45 35.34025677977654 38.0 35.0 40.0 26.5 41.0 46-47 35.05522282624071 38.0 34.5 40.0 25.5 41.0 48-49 34.82254615940545 38.0 34.0 40.0 24.0 41.0 50-51 33.8187208677291 37.5 33.0 39.5 23.0 40.5 52-53 33.90614918637537 37.5 33.5 39.5 23.0 40.5 54-55 34.33932403272502 38.0 34.0 40.0 23.0 41.0 56-57 34.21859544411362 38.0 34.0 40.0 23.0 41.0 58-59 33.98602936952986 38.0 34.0 40.0 22.0 41.0 60-61 33.68393077439438 37.0 33.0 40.0 21.5 41.0 62-63 33.32555701202027 37.0 33.0 40.0 20.0 41.0 64-65 32.96712152373833 36.0 32.5 39.5 19.5 41.0 66-67 32.50762229231161 36.0 32.0 39.0 17.5 41.0 68-69 32.12301416180932 35.0 31.5 39.0 14.0 40.0 70-71 31.731172097832136 35.0 31.0 38.0 12.0 40.0 72-73 31.33920915395213 35.0 31.0 37.0 8.0 39.5 74-75 30.916967937677406 35.0 31.0 37.0 7.0 39.0 76-77 30.461116535942054 35.0 30.0 36.0 4.5 39.0 78-79 29.98549826953132 34.0 30.0 36.0 2.0 38.5 80-81 29.5419971931858 34.0 29.0 35.0 2.0 37.0 82-83 29.13900588710846 34.0 29.0 35.0 2.0 37.0 84-85 28.787768754174 34.0 29.0 35.0 2.0 36.0 86-87 28.44527355293468 34.0 29.0 35.0 2.0 36.0 88-89 28.038814451063786 34.0 27.5 35.0 2.0 36.0 90-91 27.63237892506046 34.0 27.0 35.0 2.0 35.0 92-93 27.199895494608842 33.0 26.0 35.0 2.0 35.0 94-95 26.714617552276273 33.0 25.0 35.0 2.0 35.0 96-97 26.150875264799858 33.0 24.5 35.0 2.0 35.0 98-99 25.422176489801938 33.0 21.0 35.0 2.0 35.0 100-101 22.600644950006302 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48081481869408904 1101 2 0.5078647963506135 1101 3 0.5443050061619452 1101 4 0.6227095664051916 1101 5 0.36510332769680076 1101 6 0.40560253923558776 1101 7 0.5285421392931227 1101 8 0.5228670189970259 1101 9 0.6136241026433851 1101 10-11 0.6875225947215995 1101 12-13 0.6056860913868718 1101 14-15 0.8035439202039498 1101 16-17 0.8898321134469356 1101 18-19 0.8126885669251394 1101 20-21 0.7883176256280393 1101 22-23 0.6738256465748833 1101 24-25 0.7516607144040819 1101 26-27 0.7596690090581859 1101 28-29 0.8126927629561109 1101 30-31 0.8330842325458363 1101 32-33 0.7032328925793152 1101 34-35 0.5948361020667008 1101 36-37 0.5104277752357689 1101 38-39 0.45791698170781103 1101 40-41 0.514960427715657 1101 42-43 0.5195040306243754 1101 44-45 0.4893607355147864 1101 46-47 0.7017724247966513 1101 48-49 0.7123817350838593 1101 50-51 0.5056079502002788 1101 52-53 0.5160097949912199 1101 54-55 0.569190983775151 1101 56-57 0.599121330366934 1101 58-59 0.4727406581267175 1101 60-61 0.5095621859821122 1101 62-63 0.6267440399529463 1101 64-65 0.35035875698841323 1101 66-67 0.4762832679083431 1101 68-69 0.5063661242408486 1101 70-71 0.4727610347362514 1101 72-73 0.42059254735130835 1101 74-75 0.46905634159763565 1101 76-77 0.38693844630230956 1101 78-79 0.21194019874423375 1101 80-81 0.10679310588178481 1101 82-83 0.09342879818185068 1101 84-85 0.0634895624436318 1101 86-87 0.30352017085579774 1101 88-89 0.3423805473083448 1101 90-91 0.1637415892005798 1101 92-93 -0.10598263720022061 1101 94-95 -0.05956335317526751 1101 96-97 0.09245126197363263 1101 98-99 0.17415425117851058 1101 100-101 0.39284805868423334 1102 1 0.4096835911195562 1102 2 0.5167155610068654 1102 3 0.29802881334941844 1102 4 0.32335736085782685 1102 5 0.23015288949876123 1102 6 0.5300324504637572 1102 7 0.5543784357311949 1102 8 0.48144429739767247 1102 9 0.428320922438985 1102 10-11 0.5751134086026752 1102 12-13 0.528606908422006 1102 14-15 0.5034878481081648 1102 16-17 0.3690280180273291 1102 18-19 0.5075215772149093 1102 20-21 0.4974509073822375 1102 22-23 0.35975775081507066 1102 24-25 0.44791439750508744 1102 26-27 0.13852308951625503 1102 28-29 0.09827765152629553 1102 30-31 0.4018836157527801 1102 32-33 0.28930409874939755 1102 34-35 0.3672949982551543 1102 36-37 0.4478046794630046 1102 38-39 0.3335118715989225 1102 40-41 0.2765240843711183 1102 42-43 0.21157575977458265 1102 44-45 0.12639365900063382 1102 46-47 0.21783330615294716 1102 48-49 0.29632031457278885 1102 50-51 0.4642965640603407 1102 52-53 0.5129926849146855 1102 54-55 0.3983719268107606 1102 56-57 0.4064473653418972 1102 58-59 0.37189283725549416 1102 60-61 0.3361872658444085 1102 62-63 0.45124916327112174 1102 64-65 0.5522102144585403 1102 66-67 0.5635745813163169 1102 68-69 0.7112767458593368 1102 70-71 0.5046318209047769 1102 72-73 0.12783070009924558 1102 74-75 0.2322789774713776 1102 76-77 0.4861723073783004 1102 78-79 0.3441716794519323 1102 80-81 0.507975472285974 1102 82-83 0.45800794744954487 1102 84-85 0.11061654147859556 1102 86-87 0.03225038050613094 1102 88-89 0.06550732146450144 1102 90-91 0.22913297512486608 1102 92-93 0.48395080414378455 1102 94-95 0.13294724795533952 1102 96-97 0.1568861873411329 1102 98-99 -0.0894018519186659 1102 100-101 0.2086156398472454 1103 1 0.2764248390561228 1103 2 0.1851556585212677 1103 3 0.20754739975497927 1103 4 0.34470776771444633 1103 5 0.3282248300572519 1103 6 0.36236671059690906 1103 7 0.45777139764008723 1103 8 0.44289039663934915 1103 9 0.09988760283152232 1103 10-11 0.4815855809218448 1103 12-13 0.12793968543250855 1103 14-15 0.2909111867010665 1103 16-17 0.4099523641961653 1103 18-19 0.5778460057389694 1103 20-21 0.5433311424954397 1103 22-23 0.24270402679248804 1103 24-25 0.3493851304300506 1103 26-27 0.35449417044664955 1103 28-29 0.3041930151464314 1103 30-31 0.24163895517651213 1103 32-33 0.21493479027810736 1103 34-35 0.4549686821204517 1103 36-37 0.5966963459171026 1103 38-39 0.5414095907967962 1103 40-41 0.43558078921513044 1103 42-43 0.3318981840788524 1103 44-45 0.5657824084039262 1103 46-47 0.7087633005775729 1103 48-49 0.6526305094922762 1103 50-51 0.753665817871088 1103 52-53 0.7186256357457097 1103 54-55 0.6247449084703263 1103 56-57 0.6921816932541276 1103 58-59 0.5528192240844874 1103 60-61 0.5925816379952309 1103 62-63 0.7861470027221813 1103 64-65 0.6990338904891615 1103 66-67 0.7596747340014218 1103 68-69 0.4898432549821834 1103 70-71 0.5291689372333366 1103 72-73 0.6537015335995946 1103 74-75 0.46521851198221853 1103 76-77 0.5537491327670274 1103 78-79 0.5574844361287141 1103 80-81 0.5931548135171489 1103 82-83 0.4707402091933588 1103 84-85 0.4865170738722533 1103 86-87 0.5769062195271957 1103 88-89 0.5919520906330433 1103 90-91 0.3599830193999054 1103 92-93 0.2545569166497508 1103 94-95 0.543761289154812 1103 96-97 0.6270797949732021 1103 98-99 0.5121191687029345 1103 100-101 0.7155812146392613 1104 1 0.31085930647518367 1104 2 0.3034373479991004 1104 3 0.20301262596408876 1104 4 0.27890941608118425 1104 5 0.35121105313202605 1104 6 0.3571469152893769 1104 7 0.4914512135816196 1104 8 0.42079955601938934 1104 9 0.35790975888893684 1104 10-11 0.4984563810583609 1104 12-13 0.4041678696792701 1104 14-15 0.3455721770548621 1104 16-17 0.3808423822018909 1104 18-19 0.34623468998768914 1104 20-21 0.0829773144167163 1104 22-23 0.20090986864813942 1104 24-25 0.34555989574765533 1104 26-27 0.18404689111478234 1104 28-29 0.20946562134682978 1104 30-31 0.37649968544769763 1104 32-33 0.39477843669128276 1104 34-35 0.5680416092070928 1104 36-37 0.41240839562701126 1104 38-39 0.25986967027301944 1104 40-41 0.2463039993328593 1104 42-43 0.4915219273070903 1104 44-45 0.3750433893429701 1104 46-47 0.21134745475988126 1104 48-49 0.572272140755544 1104 50-51 0.5085864985578965 1104 52-53 0.4254619249199223 1104 54-55 0.3935023373006601 1104 56-57 0.4048311034589389 1104 58-59 0.3793376475837178 1104 60-61 0.286683735131966 1104 62-63 0.4081470301818868 1104 64-65 0.509673005266805 1104 66-67 0.48127747840258195 1104 68-69 0.3851237166190131 1104 70-71 0.2921424209673802 1104 72-73 0.2841410065302128 1104 74-75 0.5371533716827521 1104 76-77 0.3840028113865124 1104 78-79 0.4566227408451162 1104 80-81 0.6537922120633546 1104 82-83 0.5501509730046124 1104 84-85 0.5272589369738689 1104 86-87 0.4252526550616196 1104 88-89 0.4422243830082664 1104 90-91 0.3963997341210508 1104 92-93 0.21663604766783706 1104 94-95 0.24723474542915724 1104 96-97 0.2615053379888259 1104 98-99 0.5286258826002914 1104 100-101 0.5043932980853754 1105 1 0.30392703202366533 1105 2 0.25886559618561833 1105 3 0.3872410545746021 1105 4 0.4212391779067204 1105 5 0.29567614034928624 1105 6 0.2663905024733566 1105 7 0.34010302484629307 1105 8 0.45404951719865494 1105 9 0.5510614111734427 1105 10-11 0.4485842121992505 1105 12-13 0.42826306921785573 1105 14-15 0.40652720415543797 1105 16-17 0.5204514573810215 1105 18-19 0.6045025826501984 1105 20-21 0.5185076241692244 1105 22-23 0.2565039368395361 1105 24-25 0.2946002841029127 1105 26-27 0.18351264383038313 1105 28-29 0.14944090547765398 1105 30-31 0.1810562232295183 1105 32-33 0.0483149133951386 1105 34-35 0.12542928787491547 1105 36-37 0.22086687962178075 1105 38-39 0.21834904352147078 1105 40-41 -0.07237727871974897 1105 42-43 0.02040547988515584 1105 44-45 0.21914855287069912 1105 46-47 0.238719771507526 1105 48-49 0.3452604871400382 1105 50-51 0.4338038946846936 1105 52-53 0.5866068039101364 1105 54-55 0.5233438377775954 1105 56-57 0.2908598899596697 1105 58-59 0.5088547176163232 1105 60-61 0.5304932140467642 1105 62-63 0.5783879775557565 1105 64-65 0.5097548061053345 1105 66-67 0.5900864581936105 1105 68-69 0.5174710032343626 1105 70-71 0.48642865550378644 1105 72-73 0.3753789813448023 1105 74-75 0.5366232837630243 1105 76-77 0.34277472661680974 1105 78-79 0.4245323181057792 1105 80-81 0.5791057357292111 1105 82-83 0.43509416532623746 1105 84-85 0.3390291995499375 1105 86-87 0.34284639789827054 1105 88-89 0.23523082725059652 1105 90-91 0.1216584466035826 1105 92-93 0.07739161012485951 1105 94-95 0.33496406506801435 1105 96-97 0.29060887474281927 1105 98-99 0.41480070901613786 1105 100-101 0.6316342713397383 1106 1 0.28319037404815717 1106 2 0.255773556372894 1106 3 0.09861248126006572 1106 4 0.34354165842233897 1106 5 0.21514024336070037 1106 6 0.29711711768461413 1106 7 0.41201298887150273 1106 8 0.40003500469705955 1106 9 0.49798610930115217 1106 10-11 0.4464464707367739 1106 12-13 0.5453192662634905 1106 14-15 0.4909602123169492 1106 16-17 0.3533697998062806 1106 18-19 0.4007952467173794 1106 20-21 0.10068761125036474 1106 22-23 0.44775541614342984 1106 24-25 0.3837993618659752 1106 26-27 0.32137386508709653 1106 28-29 0.3936507568164487 1106 30-31 0.5524876236057636 1106 32-33 0.4120317734518153 1106 34-35 0.3307220132359632 1106 36-37 0.3893547599384277 1106 38-39 0.5219481722055832 1106 40-41 0.3526662163508121 1106 42-43 0.25473711742152005 1106 44-45 0.1600341549414921 1106 46-47 0.35613430192578477 1106 48-49 0.3763624094099782 1106 50-51 0.03528296282932075 1106 52-53 0.05604968981396752 1106 54-55 0.22230938590978866 1106 56-57 0.2636680448728441 1106 58-59 0.23504393649673005 1106 60-61 0.28610325303909434 1106 62-63 0.3732357565960811 1106 64-65 0.521967373233899 1106 66-67 0.4055049302789797 1106 68-69 0.3584353443488766 1106 70-71 0.26088325796591505 1106 72-73 -0.03025133177256123 1106 74-75 0.34617381112602175 1106 76-77 0.19386434296435695 1106 78-79 0.09614288516833724 1106 80-81 0.35718752060660464 1106 82-83 -0.019382880120808466 1106 84-85 -0.0702073146207347 1106 86-87 0.09066315752730247 1106 88-89 0.1486782300408862 1106 90-91 -0.0011134034810496019 1106 92-93 -0.04614169837659787 1106 94-95 -0.1966305655892917 1106 96-97 -0.0630310379369945 1106 98-99 0.06159126787474989 1106 100-101 -0.47304834430528686 1107 1 0.15436027896754467 1107 2 0.20287330243088775 1107 3 0.1708666594919208 1107 4 0.09774705616639068 1107 5 0.12385576925687758 1107 6 0.22942208431781097 1107 7 0.23393940474583275 1107 8 0.4185030847773774 1107 9 0.30257123892968707 1107 10-11 0.3500630773628899 1107 12-13 0.3953174945750746 1107 14-15 0.02220689040116497 1107 16-17 0.09279222938166498 1107 18-19 0.20380947928101278 1107 20-21 0.18008878056471644 1107 22-23 0.16012770693655654 1107 24-25 0.0807414276546723 1107 26-27 0.2868708236886448 1107 28-29 0.16431395884799116 1107 30-31 0.19349630487901948 1107 32-33 -0.08605952755804225 1107 34-35 0.12809046537084612 1107 36-37 0.09177488631887343 1107 38-39 -1.1315185901139557E-5 1107 40-41 0.0875330444379756 1107 42-43 0.12912913301237694 1107 44-45 0.20995679121385535 1107 46-47 0.16999244873969133 1107 48-49 0.1295236921124001 1107 50-51 -0.06651352922759912 1107 52-53 0.04843497298882937 1107 54-55 0.07069183438317594 1107 56-57 -0.19439136574547433 1107 58-59 -0.07367985727874071 1107 60-61 0.12122874763543479 1107 62-63 0.24341469712640418 1107 64-65 0.12884152429986528 1107 66-67 0.16028553619641173 1107 68-69 0.3450366553983102 1107 70-71 0.16299983506388926 1107 72-73 0.06820139367479072 1107 74-75 0.16315249180871305 1107 76-77 0.1886862882782374 1107 78-79 0.2502679663707248 1107 80-81 0.19847730977567934 1107 82-83 0.39474692558403035 1107 84-85 0.09639990205676341 1107 86-87 0.4459421984533023 1107 88-89 0.4061081007076268 1107 90-91 0.21113368799712973 1107 92-93 0.282818320521411 1107 94-95 0.2386295773269076 1107 96-97 0.28148912978284457 1107 98-99 0.38068062002735203 1107 100-101 0.03290098540592723 1108 1 0.33657690047847666 1108 2 0.38072724988221296 1108 3 0.3480224782809067 1108 4 0.3639735069256389 1108 5 0.28034496338453607 1108 6 0.317870197228153 1108 7 0.43783581959051787 1108 8 0.38680753944311874 1108 9 0.4820255896322294 1108 10-11 0.5562958289223445 1108 12-13 0.36033293153556656 1108 14-15 0.4184138086797873 1108 16-17 0.5786796970900596 1108 18-19 0.6097145892299736 1108 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-2.2153727123216314 2316 48-49 -2.496888589367771 2316 50-51 -2.0502689644924885 2316 52-53 -1.885017862222817 2316 54-55 -1.76812971145052 2316 56-57 -2.103188952044171 2316 58-59 -1.791227110806119 2316 60-61 -2.3811441337204897 2316 62-63 -2.338981256374346 2316 64-65 -2.2216687250567766 2316 66-67 -2.1249837867248758 2316 68-69 -2.185379530598677 2316 70-71 -1.7987642966248139 2316 72-73 -1.6806316882313226 2316 74-75 -1.769556710427512 2316 76-77 -1.4457675230626066 2316 78-79 -2.2745523371167202 2316 80-81 -2.5133143310087718 2316 82-83 -2.521055718838724 2316 84-85 -2.1371682938789895 2316 86-87 -2.220267758064576 2316 88-89 -2.3968806294370175 2316 90-91 -2.6291485020818506 2316 92-93 -3.155138907271649 2316 94-95 -2.69569290026649 2316 96-97 -2.796546188346536 2316 98-99 -4.052420808670849 2316 100-101 -5.333051627958167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2236.0 3 886.0 4 867.0 5 1074.0 6 1120.0 7 1406.0 8 1792.0 9 2203.0 10 2959.0 11 3865.0 12 5041.0 13 6472.0 14 7229.0 15 8998.0 16 11235.0 17 14950.0 18 11706.0 19 7836.0 20 7643.0 21 8222.0 22 8944.0 23 9820.0 24 11444.0 25 12854.0 26 15084.0 27 17903.0 28 21258.0 29 25590.0 30 31162.0 31 38649.0 32 48434.0 33 62091.0 34 83503.0 35 115716.0 36 161807.0 37 210053.0 38 161101.0 39 23287.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.83376203802782 18.382943040579473 13.914039564298845 21.869255357093863 2 15.672033376603112 20.369776546180056 40.17873218979942 23.77945788741741 3 19.774360082415672 25.780963968436478 29.526967296959654 24.917708652188193 4 12.490696693339004 16.447731708069956 37.298285447741996 33.76328615084904 5 13.340902737162743 37.946124596732005 33.43226727084688 15.280705395258373 6 30.823200856342975 36.01898295548114 17.928566037217983 15.229250150957895 7 28.51169557803154 33.489914530500755 20.31317382570532 17.68521606576239 8 27.12855359200922 33.31020868777784 19.658635640195378 19.902602080017562 9 27.46422283340923 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22.034064682946568 34-35 22.35768106051968 26.231410428420666 29.826687367707233 21.58422114335242 36-37 22.369121561668145 25.941214545049274 29.87680516440981 21.812858728872772 38-39 22.004533428894266 26.340053461540442 29.89032497003719 21.765088139528103 40-41 22.046244345481895 26.356644972349496 29.88320390043333 21.71390678173528 42-43 21.765230620318697 26.691249249298167 29.278207420470615 22.26531270991252 44-45 22.022243902773585 26.61059538832954 29.245511097089246 22.12164961180763 46-47 22.47331504683718 27.03936654822881 28.494398956820977 21.99291944811303 48-49 22.511940166712247 27.080409553505064 28.12846942108516 22.279180858697526 50-51 22.312651640503766 27.386725079517493 28.30964240704383 21.990980872934905 52-53 22.657852140931816 27.524331108840133 27.85380415772183 21.964012592506226 54-55 22.012114353322445 27.391298756414052 28.045885363752106 22.550701526511403 56-57 22.430815129796645 27.645485408593668 27.55688248002469 22.366816981584993 58-59 22.699131925838604 27.112035151645053 27.73469081556103 22.45414210695531 60-61 22.299438878958174 27.270011102394946 28.383979972822708 22.046570045824172 62-63 22.34584618761094 27.33448469939101 28.317646720942903 22.002022392055146 64-65 22.011576263986484 27.627003052454114 28.10323904775091 22.258181635808487 66-67 21.999764204027823 27.66285463178315 27.732564495557387 22.604816668631635 68-69 22.083729107925855 27.51014719598615 28.069170320657825 22.33695337543017 70-71 22.232448822916353 27.57909770501928 27.789510252572256 22.39894321949211 72-73 22.242405352812032 27.428960215319755 27.874570584485266 22.454063847382947 74-75 22.10052518918474 28.21489705468691 27.485215118313356 22.199362637814993 76-77 22.268981967222253 27.72739492670821 27.56883579954662 22.43478730652292 78-79 22.233778956231053 27.76066666294945 27.652366959712083 22.35318742110742 80-81 22.388509837972894 28.05542590031266 27.300417680551597 22.255646581162846 82-83 22.271665826802046 27.930737972959935 27.497282055130857 22.300314145107166 84-85 22.114331355056223 27.935293489691965 27.365904666278812 22.584470488972997 86-87 22.29816982614034 27.955854258343994 27.261993366648717 22.483982548866948 88-89 22.25864194222627 27.878407202958357 27.02764349643604 22.835307358379335 90-91 22.117911823215884 27.74484128804951 27.46112767584262 22.676119212891987 92-93 22.270762391749326 27.80006842977673 27.511171289678337 22.41799788879561 94-95 21.92161023761294 27.735399719194454 27.598683857968126 22.744306185224485 96-97 21.89670458949708 27.599062961959408 27.632804220101043 22.87142822844247 98-99 21.818359600319244 27.867521585617048 27.08986898743053 23.224249826633176 100-101 21.875293557498665 27.72636902349103 27.299742848992953 23.09859457001735 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 201.0 2 317.0 3 463.5 4 375.5 5 354.0 6 562.0 7 1024.5 8 1568.5 9 1873.5 10 1732.0 11 1538.5 12 1602.5 13 1684.5 14 1817.5 15 1986.0 16 2039.5 17 2162.0 18 2303.5 19 2445.5 20 2636.0 21 3001.5 22 3554.5 23 4121.5 24 4767.0 25 5742.5 26 7191.0 27 8785.5 28 10323.5 29 12159.5 30 14437.5 31 17094.0 32 20255.5 33 22425.5 34 23988.5 35 25548.5 36 27838.0 37 30503.0 38 31466.0 39 31585.0 40 31912.0 41 33351.0 42 34738.0 43 35899.0 44 37327.0 45 39265.0 46 40371.5 47 42784.5 48 50804.5 49 50258.0 50 42470.5 51 38935.0 52 36919.5 53 36937.0 54 36559.0 55 34456.0 56 31557.5 57 28489.0 58 26901.0 59 24483.0 60 20541.0 61 17472.5 62 14918.5 63 11433.5 64 8185.5 65 6352.5 66 4653.0 67 3504.0 68 2632.0 69 1884.5 70 1465.0 71 1173.5 72 994.5 73 912.0 74 714.5 75 495.5 76 360.0 77 232.0 78 151.0 79 108.5 80 70.5 81 42.5 82 31.0 83 22.0 84 13.0 85 12.0 86 11.5 87 8.0 88 4.5 89 3.0 90 4.0 91 3.5 92 4.0 93 2.5 94 1.5 95 2.5 96 1.5 97 0.5 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012259451136656873 2 0.010373381731017355 3 0.014059790114767323 4 0.01671743336816846 5 0.0299199192076451 6 0.047923308988749594 7 0.0056582082169185565 8 0.02597631954130792 9 0.03514947528691831 10-11 0.03845009674678747 12-13 0.030520032200348584 14-15 0.026919354244127676 16-17 0.027819523733182906 18-19 0.02233277637132249 20-21 0.052852808571671066 22-23 0.012730968488066754 24-25 0.0030005649635174163 26-27 0.0012430911991715011 28-29 0.0 30-31 0.0022718563295203295 32-33 0.0016288781230523117 34-35 0.0 36-37 1.7146085505813808E-4 38-39 8.573042752906904E-5 40-41 0.001071630344113363 42-43 0.003300621459869158 44-45 0.0012859564129360356 46-47 0.001071630344113363 48-49 5.143825651744143E-4 50-51 0.0031720258185755548 52-53 0.0033434866736336928 54-55 0.0053152865068022805 56-57 6.001129927034833E-4 58-59 0.0061725907820929715 60-61 0.0027433736809302093 62-63 5.572477789389488E-4 64-65 7.287086339970869E-4 66-67 0.01581726387911324 68-69 0.04153639213783395 70-71 0.05503893447366232 72-73 0.015560072596526031 74-75 0.033520597163865996 76-77 0.027476602023066628 78-79 0.06005416448411286 80-81 0.08255840171049349 82-83 0.05010943489074086 84-85 0.01684602900946207 86-87 0.01877496362886612 88-89 0.02224704594379342 90-91 0.04136493128277582 92-93 0.024690363128371886 94-95 0.013674003190886514 96-97 0.01877496362886612 98-99 0.04856628719521761 100-101 0.0676841725342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1166447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.739862030910764 #Duplication Level Percentage of deduplicated Percentage of total 1 81.92319962970434 49.76003842639002 2 10.91972857667863 13.265256143249069 3 3.0538019270309014 5.564625231527591 4 1.2763667697178074 3.101053659739955 5 0.6898568199852976 2.095090403349491 6 0.4222122418097744 1.5387067989172338 7 0.28185057707770333 1.1983695615522596 8 0.21534130405709176 1.0463840878387325 9 0.1607877834814306 0.8789605006426242 >10 0.9422894437801236 10.695927538971953 >50 0.07108058658825007 2.984463753334384 >100 0.03868412755725772 4.329475836904837 >500 0.0033883853162226278 1.3622910664103 >1k 0.0012706444935834855 1.668433525625003 >5k 1.4118272150927616E-4 0.5109234655465547 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5958 0.5107818872181934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4808 0.412191895559764 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3206 0.2748517506581954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2935 0.2516188047978177 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1861 0.15954432563159748 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1500 0.12859564129360357 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1323 0.11342135562095834 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1293 0.11084944279508627 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1276 0.10939202552709211 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1254 0.10750595612145257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.573042752906904E-5 0.0 0.0 0.0 0.0 14-15 8.573042752906904E-5 0.0 0.0 0.0 0.0 16-17 8.573042752906904E-5 0.0 0.0 0.0 0.0 18-19 1.2859564129360357E-4 0.0 0.0 0.0 0.0 20-21 1.7146085505813808E-4 0.0 0.0 0.0 0.0 22-23 1.7146085505813808E-4 0.0 0.0 0.0 4.286521376453452E-5 24-25 2.5719128258720715E-4 0.0 0.0 0.0 1.7146085505813808E-4 26-27 2.5719128258720715E-4 0.0 0.0 5.143825651744143E-4 1.7146085505813808E-4 28-29 2.5719128258720715E-4 0.0 0.0 0.0014574172679941736 1.7146085505813808E-4 30-31 2.5719128258720715E-4 0.0 0.0 0.0026576432534011407 1.7146085505813808E-4 32-33 2.5719128258720715E-4 0.0 0.0 0.004115060521395314 1.7146085505813808E-4 34-35 3.0005649635174165E-4 0.0 0.0 0.0063440516371511095 1.7146085505813808E-4 36-37 3.4292171011627616E-4 0.0 0.0 0.009430347028197596 1.7146085505813808E-4 38-39 4.286521376453452E-4 0.0 0.0 0.014145520542296394 1.7146085505813808E-4 40-41 4.286521376453452E-4 0.0 0.0 0.02027524611062483 1.7146085505813808E-4 42-43 4.286521376453452E-4 0.0 0.0 0.02931980621494161 1.7146085505813808E-4 44-45 4.286521376453452E-4 0.0 0.0 0.03819290546420026 2.143260688226726E-4 46-47 5.143825651744143E-4 0.0 0.0 0.05461028233601698 2.5719128258720715E-4 48-49 5.143825651744143E-4 0.0 0.0 0.07355670681994124 2.5719128258720715E-4 50-51 5.143825651744143E-4 0.0 0.0 0.08165823222143825 2.5719128258720715E-4 52-53 5.143825651744143E-4 0.0 0.0 0.09396054857185968 2.5719128258720715E-4 54-55 5.143825651744143E-4 0.0 0.0 0.11372141211731009 2.5719128258720715E-4 56-57 6.001129927034833E-4 0.0 0.0 0.15092841766492604 3.857869238808107E-4 58-59 6.001129927034833E-4 0.0 0.0 0.20043773956296343 5.143825651744143E-4 60-61 6.001129927034833E-4 0.0 0.0 0.2520903221492275 5.143825651744143E-4 62-63 6.858434202325523E-4 0.0 0.0 0.31197302577828223 5.143825651744143E-4 64-65 6.858434202325523E-4 0.0 0.0 0.3751992160809707 5.143825651744143E-4 66-67 6.858434202325523E-4 0.0 0.0 0.4645303215662606 5.143825651744143E-4 68-69 6.858434202325523E-4 0.0 0.0 0.5727221211079458 5.143825651744143E-4 70-71 7.287086339970868E-4 0.0 0.0 0.6335049942260558 5.143825651744143E-4 72-73 8.144390615261559E-4 0.0 0.0 0.7135343483244416 5.572477789389488E-4 74-75 8.573042752906904E-4 0.0 0.0 0.8000792149150369 6.001129927034833E-4 76-77 8.573042752906904E-4 0.0 0.0 0.9011125237580447 6.001129927034833E-4 78-79 8.573042752906904E-4 0.0 0.0 1.0075897147491486 6.001129927034833E-4 80-81 0.0010287651303488286 0.0 0.0 1.1664481969605134 6.001129927034833E-4 82-83 0.0010287651303488286 0.0 0.0 1.3376518607360643 6.858434202325523E-4 84-85 0.0010287651303488286 0.0 0.0 1.5902565654504661 6.858434202325523E-4 86-87 0.0010287651303488286 0.0 0.0 1.9537964433874837 6.858434202325523E-4 88-89 0.0010287651303488286 0.0 0.0 2.3347824633266665 6.858434202325523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1055 0.0 34.216293 2 GTACCTG 1290 0.0 27.984293 1 GTATCAA 12100 0.0 23.514294 1 GTACATG 7515 0.0 23.38639 1 TACATGG 7625 0.0 22.736565 2 GAGTACT 5615 0.0 22.54726 12-13 TATAACG 150 3.0344361E-5 22.165554 2 TATAGGG 215 4.423964E-8 22.091917 2 ATTCGCG 140 6.548362E-11 22.064295 80-81 ACATGGG 7555 0.0 21.692636 3 TAAGGTA 290 1.05501385E-10 21.295689 4 GTACTTT 6035 0.0 21.057274 14-15 CTAGTAC 250 9.480573E-9 20.901642 3 CGTTTAG 80 4.982504E-5 20.786448 90-91 ATCAACG 13775 0.0 20.553339 3 TAACGCA 185 6.5453532E-6 20.543036 4 TCAACGC 13795 0.0 20.524422 4 CAACGCA 13925 0.0 20.366926 5 TATCAAC 13945 0.0 20.334227 2 AACGCAG 14115 0.0 20.194603 6 >>END_MODULE