FastQCFastQC Report
Thu 26 May 2016
SRR936479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166447
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75330.6458073105764771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67800.5812522986470882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41500.35578127424563655No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36570.3135161734738055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.16708860325415556No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17730.1520000480090394No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14660.12568080675761523No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13710.11753641614235366No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12960.11110663407767349No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11870.10176201747700495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.10021886978148171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10600.029.5780622
GTACACG1951.6370905E-1129.2358361
GTACCTG12500.026.2245451
GTACATG71700.025.3112221
TACATGG73300.024.497372
GAGTACT75850.024.04853612-13
GGTATCA102350.023.6264691
ACATGGG72950.022.9839783
GTATCAA135000.022.8391441
GTAAGGT4700.022.2321764
GTACTTT82250.022.09065214-15
TAAGGTG5550.021.39465
CATGGGA45450.020.9003354
GCACCGT3451.8189894E-1220.6504426
AGTACTT77800.020.6066412-13
CCTATAC2801.5770638E-920.3563083
ATATAGG4500.020.0573122
CTACACG2401.4144825E-719.7900054
TCAACGC155800.019.7544384
ATCAACG155750.019.7006363
Produced by FastQC (version 0.11.5)