##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936479_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1166447 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.959719558625466 31.0 31.0 34.0 30.0 34.0 2 32.0703315281363 33.0 31.0 34.0 30.0 34.0 3 32.17455229427484 33.0 31.0 34.0 30.0 34.0 4 35.91032254358749 37.0 35.0 37.0 35.0 37.0 5 35.78371756282112 37.0 35.0 37.0 35.0 37.0 6 35.84943765126062 37.0 35.0 37.0 35.0 37.0 7 35.77924414911265 37.0 35.0 37.0 33.0 37.0 8 35.75355931302494 37.0 35.0 37.0 33.0 37.0 9 37.36489184677915 39.0 37.0 39.0 34.0 39.0 10-11 37.27051550563378 39.0 37.0 39.0 34.0 39.0 12-13 37.149254102415284 39.0 37.0 39.0 33.0 39.0 14-15 38.218132499804966 40.0 37.5 41.0 33.0 41.0 16-17 38.206349281193226 40.0 37.5 41.0 33.0 41.0 18-19 38.38855001556007 40.0 38.0 41.0 33.5 41.0 20-21 38.40630778766631 40.0 38.0 41.0 34.0 41.0 22-23 38.30504600723393 40.0 38.0 41.0 34.0 41.0 24-25 38.18477221854057 40.0 37.5 41.0 33.0 41.0 26-27 37.973555163672245 40.0 37.0 41.0 33.0 41.0 28-29 37.96148303351974 40.0 37.0 41.0 33.0 41.0 30-31 37.787394969509975 40.0 37.0 41.0 33.0 41.0 32-33 37.53781954945231 40.0 36.0 41.0 33.0 41.0 34-35 37.31281189801165 39.0 36.0 41.0 32.0 41.0 36-37 37.09307581055976 39.0 36.0 41.0 31.5 41.0 38-39 36.86938112061671 39.0 35.0 41.0 31.0 41.0 40-41 36.614990222444746 39.0 35.0 41.0 30.5 41.0 42-43 36.29887898892963 39.0 35.0 40.0 30.0 41.0 44-45 35.92392710513208 39.0 35.0 40.0 29.5 41.0 46-47 35.42149493290308 38.0 35.0 40.0 27.5 41.0 48-49 34.92208218633166 38.0 34.5 40.0 25.5 41.0 50-51 34.58177568290715 38.0 34.0 40.0 24.0 41.0 52-53 34.29459075294463 38.0 34.0 40.0 23.5 41.0 54-55 33.963324094450925 38.0 33.0 40.0 23.0 41.0 56-57 33.61942805802578 37.5 33.0 40.0 22.0 41.0 58-59 33.24992734346267 37.0 33.0 40.0 20.5 41.0 60-61 32.866690899800844 36.5 32.0 39.0 19.5 40.5 62-63 32.53685593944688 36.0 32.0 39.0 18.0 40.0 64-65 32.21037089554862 35.5 31.0 39.0 17.0 40.0 66-67 31.763729942294848 35.0 31.0 38.5 13.5 40.0 68-69 31.31800287539854 35.0 30.5 38.0 9.5 40.0 70-71 30.811552946683392 34.5 30.0 37.0 7.5 39.5 72-73 30.25261756427853 34.0 29.0 36.5 7.0 39.0 74-75 29.733558404282405 34.0 29.0 36.0 3.5 39.0 76-77 28.3784128211569 32.5 27.0 34.5 2.0 36.5 78-79 29.150247289418207 34.0 29.0 35.0 2.0 37.0 80-81 28.9931042730617 34.0 29.0 35.0 2.0 37.0 82-83 28.591556238731805 34.0 28.5 35.0 2.0 36.0 84-85 28.158995222243274 33.0 27.5 35.0 2.0 36.0 86-87 27.74748359762595 33.0 27.0 35.0 2.0 35.5 88-89 27.24753889375171 33.0 26.0 35.0 2.0 35.0 90-91 26.67145313931966 32.0 25.0 34.0 2.0 35.0 92-93 26.09990038124321 32.0 24.5 34.0 2.0 35.0 94-95 25.366821210050695 32.0 21.5 34.0 2.0 35.0 96-97 24.54888091786425 31.0 18.0 34.0 2.0 35.0 98-99 23.41935767334478 31.0 2.0 34.0 2.0 35.0 100-101 19.697404597036986 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3747873353455411 1101 2 0.4818219938922894 1101 3 0.36446665987247684 1101 4 0.2722335616595828 1101 5 0.380184588476304 1101 6 0.36940775800901093 1101 7 0.34961072205567234 1101 8 0.30403163549313916 1101 9 0.2960603026073869 1101 10-11 0.3716230653105157 1101 12-13 0.4259253787148296 1101 14-15 0.47623141496682564 1101 16-17 0.5891279054153244 1101 18-19 0.4972160586438932 1101 20-21 0.3806175710717099 1101 22-23 0.519284010326551 1101 24-25 0.5513998074747661 1101 26-27 0.4427409552270589 1101 28-29 0.4163427340263368 1101 30-31 0.5280283945852489 1101 32-33 0.30806143215696835 1101 34-35 0.4843190923719405 1101 36-37 0.38051366168198797 1101 38-39 0.32231148001443444 1101 40-41 0.3971042494363033 1101 42-43 0.5427336497441573 1101 44-45 0.4225240607754799 1101 46-47 0.31575483450702535 1101 48-49 0.2409352384174568 1101 50-51 0.2805994722245657 1101 52-53 0.15424933719610578 1101 54-55 0.22362954300270843 1101 56-57 0.1271425812360718 1101 58-59 0.030683812759285445 1101 60-61 0.1716855844263918 1101 62-63 0.14618178847856456 1101 64-65 0.3062514247170114 1101 66-67 0.29744432059115056 1101 68-69 0.2339530859519492 1101 70-71 0.2160504466381603 1101 72-73 0.39471867980039477 1101 74-75 0.6467316519217121 1101 76-77 0.4682558629084461 1101 78-79 0.5991141545069425 1101 80-81 0.450795884755685 1101 82-83 0.40308145023027464 1101 84-85 0.3136758297143203 1101 86-87 0.4858416934165426 1101 88-89 0.19958441609445998 1101 90-91 0.22795014988694717 1101 92-93 0.3128167765731362 1101 94-95 0.418460415107031 1101 96-97 0.62538897513706 1101 98-99 0.4948579856811257 1101 100-101 0.4499263288015065 1102 1 0.3420924511155512 1102 2 0.35812155906397436 1102 3 0.2376822597765056 1102 4 0.16169335554072006 1102 5 -0.02095464554651727 1102 6 0.12522779893085811 1102 7 0.2080329934607903 1102 8 0.18818075491861208 1102 9 0.10950520503385519 1102 10-11 0.2168365840771429 1102 12-13 0.31876324678905377 1102 14-15 0.4939156913267766 1102 16-17 0.46687321409491034 1102 18-19 0.296597108971298 1102 20-21 0.2904269529038146 1102 22-23 0.1860273268292687 1102 24-25 0.147509805830353 1102 26-27 0.25709487259081953 1102 28-29 0.42081151831320796 1102 30-31 0.533050082085758 1102 32-33 0.21663641914986442 1102 34-35 0.3530181927922129 1102 36-37 0.48396128684003514 1102 38-39 0.4241788425501838 1102 40-41 0.39798819367691607 1102 42-43 0.5288277699918424 1102 44-45 0.7054390549391272 1102 46-47 0.5484998601303559 1102 48-49 0.480245282062036 1102 50-51 0.30611308453455166 1102 52-53 0.34383232794151297 1102 54-55 0.4776954438898926 1102 56-57 0.6128962252483561 1102 58-59 0.4639334872445957 1102 60-61 0.3971582209740774 1102 62-63 0.35937787098002616 1102 64-65 0.3422028037401219 1102 66-67 0.2733600075686766 1102 68-69 0.42235991057299316 1102 70-71 -0.053806219067009664 1102 72-73 0.11441157724506823 1102 74-75 0.2744983920032844 1102 76-77 0.25110157568452607 1102 78-79 0.22271808063195664 1102 80-81 0.3411929991993716 1102 82-83 0.2986169791815385 1102 84-85 -0.045266995618405304 1102 86-87 0.12977148468277377 1102 88-89 -0.06512089538873056 1102 90-91 0.025178894384200845 1102 92-93 0.24715230875932548 1102 94-95 0.1957256064665387 1102 96-97 0.06828547629219983 1102 98-99 0.05951423408050971 1102 100-101 0.44763276442238364 1103 1 0.20878632658426355 1103 2 0.3056474408295813 1103 3 0.27418092423731366 1103 4 0.21288477310715592 1103 5 0.265404634537731 1103 6 0.2111213712157749 1103 7 0.19270617809316093 1103 8 0.19917116745993013 1103 9 0.18693368171749114 1103 10-11 0.3351037720838974 1103 12-13 0.22326763967443952 1103 14-15 0.3452098707080964 1103 16-17 0.355875877885218 1103 18-19 0.2596338299219525 1103 20-21 0.25927773327412496 1103 22-23 0.2061442275037777 1103 24-25 0.2557558948687273 1103 26-27 0.2565519112285273 1103 28-29 0.4515143435343063 1103 30-31 0.5073450522228811 1103 32-33 0.48658842719589046 1103 34-35 0.5048615283969937 1103 36-37 0.5838497793387845 1103 38-39 0.5119108309913045 1103 40-41 0.7484515328528474 1103 42-43 0.9063699405983243 1103 44-45 0.7742767167798732 1103 46-47 0.8554461215316351 1103 48-49 0.930019534032553 1103 50-51 0.8256524055048828 1103 52-53 0.9232176519509778 1103 54-55 0.8152880479544464 1103 56-57 0.769028591211601 1103 58-59 0.9762448628317415 1103 60-61 0.7882871797636781 1103 62-63 0.9368974485633714 1103 64-65 0.9459138899908126 1103 66-67 0.8302725616370559 1103 68-69 0.8184939527927959 1103 70-71 0.43282016885519425 1103 72-73 0.5610738224814042 1103 74-75 0.6815936944764935 1103 76-77 0.5695210843955962 1103 78-79 0.6171157397032374 1103 80-81 0.6492707337750758 1103 82-83 0.4540988847208389 1103 84-85 0.4504913464909812 1103 86-87 0.4547830544970388 1103 88-89 0.5492872585061974 1103 90-91 0.527476459074947 1103 92-93 0.7562004501187687 1103 94-95 0.6111621073174192 1103 96-97 0.8992204266057691 1103 98-99 0.933410546680495 1103 100-101 0.7348210626940173 1104 1 0.22430792364058405 1104 2 0.2830240781143658 1104 3 0.16233216051237065 1104 4 0.14311996546237538 1104 5 0.10791784461622456 1104 6 0.04117376040606757 1104 7 0.1649625614933754 1104 8 0.20659019075752383 1104 9 0.18785799493070243 1104 10-11 0.2503273332935336 1104 12-13 0.21044175184339764 1104 14-15 0.25823493945542 1104 16-17 0.22279438925549044 1104 18-19 0.22242961015017926 1104 20-21 0.1620007550967344 1104 22-23 0.2090289673623218 1104 24-25 0.16965452541411707 1104 26-27 0.20946170806595177 1104 28-29 0.13616417348029586 1104 30-31 0.1924663554603825 1104 32-33 0.2740704312834694 1104 34-35 0.41859245861405014 1104 36-37 0.3960805761801325 1104 38-39 0.48806477596991016 1104 40-41 0.3059268975765477 1104 42-43 0.324757742213734 1104 44-45 0.4969702488430059 1104 46-47 0.457041628339681 1104 48-49 0.5748013078508976 1104 50-51 0.5489679488735675 1104 52-53 0.6149886164534166 1104 54-55 0.49360841638520725 1104 56-57 0.629184905570277 1104 58-59 0.7021862402888956 1104 60-61 0.5176512130448359 1104 62-63 0.4871933268619699 1104 64-65 0.5866608037163488 1104 66-67 0.672790675233923 1104 68-69 0.5574473066032191 1104 70-71 0.5507750388335033 1104 72-73 0.6925996699074481 1104 74-75 0.7892631894925586 1104 76-77 0.6843793825216657 1104 78-79 0.6903534642353435 1104 80-81 0.6828666995524415 1104 82-83 0.5170174000139305 1104 84-85 0.45545346190803215 1104 86-87 0.6377727982980126 1104 88-89 0.5302878410441849 1104 90-91 0.4180625373978657 1104 92-93 0.4749209064205786 1104 94-95 0.6720262935883525 1104 96-97 0.8824859442800097 1104 98-99 0.653883520448634 1104 100-101 0.19304798967866788 1105 1 0.1999192578168838 1105 2 0.18488802806889026 1105 3 0.20082846699654056 1105 4 0.19221987894758996 1105 5 0.21014670949787728 1105 6 0.14051510190284944 1105 7 0.18407807721762026 1105 8 0.18971056461970903 1105 9 0.2702402202092884 1105 10-11 0.2894188910772755 1105 12-13 0.21415698879778233 1105 14-15 0.311112018213457 1105 16-17 0.30351009575593224 1105 18-19 0.29857879639358487 1105 20-21 0.10544263860494851 1105 22-23 0.07824467755997233 1105 24-25 0.19737900170389366 1105 26-27 0.29397491451093316 1105 28-29 0.3744957691234987 1105 30-31 0.3977850737105655 1105 32-33 0.496153699915574 1105 34-35 0.39768564317152055 1105 36-37 0.5455223894412953 1105 38-39 0.4111474937172659 1105 40-41 0.5711395427845858 1105 42-43 0.3635082320944676 1105 44-45 0.5242432029901707 1105 46-47 0.5235886901644804 1105 48-49 0.426094777833228 1105 50-51 0.6960033385967961 1105 52-53 0.5847366328986254 1105 54-55 0.5393078181761339 1105 56-57 0.6946546919453098 1105 58-59 0.6435355012160073 1105 60-61 0.5350096282714389 1105 62-63 0.5732524305096405 1105 64-65 0.5271527733696217 1105 66-67 0.39991324524065774 1105 68-69 0.3544185496801866 1105 70-71 0.3511176780520593 1105 72-73 0.5553235361570721 1105 74-75 0.7303166733764037 1105 76-77 0.60149202551138 1105 78-79 0.6158264302135912 1105 80-81 0.7196521207019799 1105 82-83 0.5792830891374692 1105 84-85 0.6733704335947763 1105 86-87 0.8374541909642197 1105 88-89 0.9071720332401014 1105 90-91 0.9543955121015308 1105 92-93 0.8480029771556588 1105 94-95 0.8638631244852242 1105 96-97 0.6328875540465724 1105 98-99 0.4942193752812578 1105 100-101 0.3013818775939576 1106 1 -0.014549866576661685 1106 2 0.06795724770367428 1106 3 0.11180808442851742 1106 4 0.05732999573598363 1106 5 0.0693546984461193 1106 6 0.07616785800512105 1106 7 0.06629012570578396 1106 8 0.07893934633338517 1106 9 0.10423096955037892 1106 10-11 0.12623363743854554 1106 12-13 -0.008136769129166055 1106 14-15 0.11441127844363308 1106 16-17 0.12891003823399672 1106 18-19 0.07223516411906417 1106 20-21 0.061114442954924186 1106 22-23 -0.04213199682343571 1106 24-25 0.05423259894660504 1106 26-27 0.0023733529318192836 1106 28-29 0.11877398669786743 1106 30-31 0.0032741751896310234 1106 32-33 -0.059549930095791126 1106 34-35 0.0562036046318255 1106 36-37 -0.1114542670254437 1106 38-39 -0.07558238421363939 1106 40-41 -0.22725102173191658 1106 42-43 0.026503710493088306 1106 44-45 0.13475156488801332 1106 46-47 0.1521964235644475 1106 48-49 0.09010042629876125 1106 50-51 -0.13613562874300555 1106 52-53 -0.0730073756259344 1106 54-55 -0.061631392453158185 1106 56-57 0.0013767431209714687 1106 58-59 0.06330560206799163 1106 60-61 -0.019308288868685963 1106 62-63 0.048882460732230015 1106 64-65 0.110410589516313 1106 66-67 -0.1969419079232857 1106 68-69 -0.2969718781038502 1106 70-71 -0.1653168577412032 1106 72-73 -0.33485530048534784 1106 74-75 -0.17753294919482343 1106 76-77 -0.44072176749451586 1106 78-79 -0.3821016013574656 1106 80-81 -0.3420062084272253 1106 82-83 -0.11995780990210392 1106 84-85 -0.1680088198459302 1106 86-87 -0.15074108186421142 1106 88-89 -0.02123083719880725 1106 90-91 0.07835104104402646 1106 92-93 -0.01492696395508375 1106 94-95 0.01022886970076442 1106 96-97 -0.18992390130556203 1106 98-99 -0.19738454375425718 1106 100-101 -0.38148695824692425 1107 1 0.020505941608540468 1107 2 0.053072702732315236 1107 3 -0.037141304338980774 1107 4 0.007297514781633652 1107 5 0.014118178042174634 1107 6 -0.008022775668770521 1107 7 -0.13742097096934458 1107 8 -0.13454438366129295 1107 9 -0.05938799932695815 1107 10-11 0.010475059513780138 1107 12-13 0.18939585946588267 1107 14-15 0.07613690296380327 1107 16-17 0.11222663895448903 1107 18-19 -0.08045012496143045 1107 20-21 -0.18136592083175884 1107 22-23 -0.04171151610530188 1107 24-25 0.09334675473707676 1107 26-27 -0.04419370547484647 1107 28-29 -0.04660604046802774 1107 30-31 0.0719613540711066 1107 32-33 0.07335032384621343 1107 34-35 0.09256809999590843 1107 36-37 0.2941737965191109 1107 38-39 0.18278530018959316 1107 40-41 0.1154584804236336 1107 42-43 0.17216720780602657 1107 44-45 0.12633604489746375 1107 46-47 0.21543979449695172 1107 48-49 0.34554364368492685 1107 50-51 0.4746338745936711 1107 52-53 0.46189724848083813 1107 54-55 0.30377698172743095 1107 56-57 0.569955258446079 1107 58-59 0.4579952613132505 1107 60-61 0.3471288457205759 1107 62-63 0.2718978744214766 1107 64-65 0.29891351280220135 1107 66-67 0.4426966676392219 1107 68-69 0.3453726562373767 1107 70-71 0.16234746635966602 1107 72-73 0.41941151579056424 1107 74-75 0.5693187604844994 1107 76-77 0.35176036798058874 1107 78-79 0.3988444802583153 1107 80-81 0.4767677831867836 1107 82-83 0.5719479362740039 1107 84-85 0.6154967609725865 1107 86-87 0.7725873301124047 1107 88-89 0.7617833953648265 1107 90-91 0.60276963196117 1107 92-93 0.4383866839513715 1107 94-95 0.7372506614683196 1107 96-97 0.7922694489572386 1107 98-99 1.2477179188926435 1107 100-101 0.8608103310697963 1108 1 0.0825940267953591 1108 2 0.22531641768411248 1108 3 -0.050403228603357775 1108 4 0.032084862774823364 1108 5 0.04858607546515259 1108 6 0.06606203310194303 1108 7 0.13027986703708194 1108 8 0.03042568753774333 1108 9 0.17710119480069864 1108 10-11 -0.02317247372293707 1108 12-13 -0.03950213057885321 1108 14-15 0.12344331087760452 1108 16-17 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2316 62-63 -1.9410367249218545 2316 64-65 -1.680167150965655 2316 66-67 -1.8604003241080456 2316 68-69 -2.0864988496637658 2316 70-71 -1.771765587149961 2316 72-73 -1.575193151881809 2316 74-75 -1.658973013408481 2316 76-77 -1.722851398813269 2316 78-79 -1.7320970407284655 2316 80-81 -2.0684979901737606 2316 82-83 -2.048472686673904 2316 84-85 -1.9858609460111722 2316 86-87 -2.3344195027920236 2316 88-89 -2.042018184189704 2316 90-91 -2.5422867173156085 2316 92-93 -2.3297453597082267 2316 94-95 -2.1963530300748495 2316 96-97 -1.7101566758098237 2316 98-99 -1.1835767833879487 2316 100-101 -1.1711854094573155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 15.0 5 45.0 6 97.0 7 227.0 8 516.0 9 1054.0 10 1666.0 11 2869.0 12 4324.0 13 6015.0 14 7494.0 15 10230.0 16 13240.0 17 17330.0 18 15052.0 19 9208.0 20 7883.0 21 8110.0 22 8719.0 23 9595.0 24 11016.0 25 12809.0 26 15751.0 27 19324.0 28 23935.0 29 29263.0 30 35996.0 31 44210.0 32 55488.0 33 70547.0 34 94677.0 35 127352.0 36 177126.0 37 213577.0 38 107487.0 39 4196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47885558382185 19.027959622357752 14.894593021112263 21.598591772708136 2 15.941750636202132 21.05451789254186 39.25650092257883 23.74723054867718 3 19.963442665256043 25.50697152831779 30.208069877322018 24.321515929104148 4 12.869862814052258 17.112836770840655 36.7555806269643 33.261719788142784 5 13.088630428483 38.02591577117166 33.570560205213674 15.314893595131663 6 30.871269761935178 36.382964678206555 17.28959824149747 15.4561673183608 7 28.1027636131696 33.83008528484865 20.26869699702686 17.79845410495489 8 27.336605439698225 33.21936686885141 19.416593437211993 20.027434254238376 9 27.110150928676447 16.454354898859354 20.437911774665757 35.99758239779844 10-11 25.207960584578636 26.68350983799521 26.452894987942017 21.655634589484134 12-13 26.859557270926153 24.36930267727552 27.426406857748358 21.344733194049965 14-15 23.287362953672265 25.27855066636546 27.006329946816788 24.427756433145483 16-17 21.416917724481696 28.56113436408499 27.80251680815639 22.219431103276925 18-19 21.243062673098283 26.39246998629574 31.363126063344808 21.001341277261172 20-21 22.277937761104077 25.059647251768414 31.478017319350055 21.184397667777453 22-23 21.77706449664599 25.28911676163508 31.462599249597385 21.471219492121545 24-25 21.82213633916416 25.533544687957587 31.631694277650706 21.012624695227547 26-27 21.60999239999434 25.48093718828939 31.66171787561624 21.247352536100028 28-29 21.068184896686173 26.079689929923887 31.688179307057506 21.16394586633243 30-31 21.311637736107865 25.5780289212713 31.55157669580798 21.558756646812853 32-33 21.11213753399877 25.7778082608705 31.46396719828704 21.646087006843693 34-35 21.952506528444815 25.46800761980532 31.269193113369464 21.310292738380397 36-37 22.046173129087236 25.25535048643903 31.300259937107565 21.398216447366163 38-39 21.672337319008168 25.64237981353506 31.279409766325113 21.405873101131657 40-41 21.677281522435223 25.70708313365288 31.229751544647975 21.38588379926392 42-43 21.515922709563313 26.00714229682583 30.61427096233255 21.862664031278307 44-45 21.79649964506051 25.88464809550101 30.353542021749046 21.96531023768943 46-47 22.227928058454435 26.423703777368367 29.51604316355565 21.832325000621548 48-49 22.407122269222395 26.620934375605483 28.948214460240084 22.02372889493204 50-51 22.2075621243082 26.891892065672096 29.070716968806142 21.829828841213565 52-53 22.581230067555982 27.010819244881862 28.588354308837143 21.81959637872501 54-55 21.947627192023628 26.87880697332493 28.818889874790493 22.354675959860945 56-57 22.481578906755058 27.04051011466777 28.354079391182225 22.12383158739495 58-59 22.629144608526055 26.649248772960117 28.397753820434236 22.323852798079603 60-61 22.182184962708337 26.853212861476656 29.13696557867343 21.82763659714158 62-63 22.265206561300978 26.741497995405698 29.096258676466242 21.897036766827082 64-65 21.942913873559366 27.027562278541133 28.96915586503237 22.06036798286713 66-67 21.96998631299057 27.103193535513565 28.582273586729475 22.34454656476639 68-69 22.185514775854532 26.85660519705426 28.73404319161887 22.223836835472337 70-71 22.236144499809672 27.043902756868164 28.469490636113292 22.250462107208875 72-73 22.23865907304163 26.855147001458313 28.559940398673888 22.346253526826168 74-75 22.048493001175864 27.64202235216905 28.058698128259834 22.25078651839525 76-77 22.22629445593243 27.1669855868646 28.2268808575867 22.379839099616266 78-79 22.114591567457083 27.180881800707485 28.315411879086337 22.38911475274909 80-81 22.294137365496677 27.477043023059426 27.95195731215402 22.276862299289878 82-83 22.23219108203805 27.326326077451323 28.083135432652327 22.3583474078583 84-85 22.055687368856482 27.37931581395249 27.963323752756825 22.6016730644342 86-87 22.194312001875797 27.35326380259225 27.707675972629502 22.74474822290245 88-89 22.06490800912193 27.27996776461309 27.58294610860582 23.072178117659163 90-91 22.0113181712764 27.09647881497435 28.009708883534124 22.88249413021513 92-93 22.091027479880694 27.138064688700524 28.03615688042955 22.734750950989227 94-95 21.745772716225133 27.013936844614022 28.149142626649194 23.091147812511654 96-97 21.782372172553284 26.838587829435667 28.188286550893295 23.19075344711776 98-99 21.618046401971817 27.02116487167122 27.703541767132826 23.657246959224135 100-101 21.316544141286407 26.88878410527895 28.09319073236599 23.70148102106865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 399.0 1 321.5 2 477.5 3 651.0 4 480.5 5 393.0 6 605.0 7 1158.5 8 1823.0 9 2233.0 10 2125.5 11 1831.0 12 1825.5 13 1917.0 14 2048.0 15 2232.5 16 2384.5 17 2543.0 18 2733.5 19 2932.5 20 3161.5 21 3526.0 22 3987.0 23 4584.5 24 5190.5 25 6149.0 26 7636.5 27 9171.0 28 10722.0 29 12614.0 30 14909.0 31 17376.0 32 20566.0 33 22485.5 34 23709.0 35 25348.0 36 27562.0 37 30355.0 38 31401.0 39 31315.5 40 31724.0 41 33220.5 42 34482.0 43 35672.0 44 36947.0 45 38697.5 46 39729.5 47 42117.5 48 50558.0 49 49940.0 50 41754.5 51 38436.5 52 36445.0 53 36348.5 54 35979.0 55 33911.5 56 31182.0 57 28577.0 58 26798.0 59 24202.5 60 20487.0 61 17319.5 62 14688.5 63 11323.5 64 7988.5 65 6010.5 66 4527.0 67 3478.5 68 2564.0 69 1812.0 70 1429.5 71 1113.5 72 942.0 73 879.5 74 714.0 75 551.5 76 425.0 77 248.5 78 153.5 79 110.5 80 80.5 81 66.5 82 46.5 83 28.5 84 20.0 85 12.5 86 6.0 87 4.5 88 3.0 89 0.5 90 1.0 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013545407549592908 2 0.012945294556889426 3 0.0051438256517441425 4 8.573042752906904E-5 5 0.004543712659040659 6 0.0 7 2.5719128258720715E-4 8 0.001886069405639519 9 1.7146085505813808E-4 10-11 0.0 12-13 0.0 14-15 6.429782064680178E-4 16-17 0.004200790948924383 18-19 0.0018432041918749846 20-21 5.143825651744143E-4 22-23 5.572477789389488E-4 24-25 2.5719128258720715E-4 26-27 4.286521376453452E-5 28-29 8.573042752906904E-5 30-31 3.857869238808107E-4 32-33 0.003815004025043573 34-35 0.0011144955578778976 36-37 9.430347028197595E-4 38-39 0.005272421293037746 40-41 0.0 42-43 0.0013288216267005701 44-45 0.004372251803982522 46-47 0.0 48-49 0.0018003389781104497 50-51 0.0013288216267005701 52-53 6.001129927034833E-4 54-55 1.7146085505813808E-4 56-57 0.010373381731017355 58-59 0.001886069405639519 60-61 0.002871969322223813 62-63 1.2859564129360357E-4 64-65 0.0029148345359883477 66-67 0.001071630344113363 68-69 0.0014574172679941739 70-71 0.004372251803982522 72-73 0.0025719128258720712 74-75 0.006215455995857506 76-77 9.430347028197595E-4 78-79 0.005229556079273212 80-81 0.002228991115755795 82-83 0.0031720258185755548 84-85 0.0021003954744621915 86-87 7.287086339970869E-4 88-89 0.002314721543284864 90-91 0.007930064546438886 92-93 0.0025719128258720712 94-95 5.572477789389488E-4 96-97 1.2859564129360357E-4 98-99 8.144390615261558E-4 100-101 0.001886069405639519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1166447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.47337207643611 #Duplication Level Percentage of deduplicated Percentage of total 1 80.92632226001838 47.320349522876285 2 11.408128913694584 13.341435333328247 3 3.198011167896908 5.609954907751017 4 1.4420559426423838 3.3728749475665563 5 0.7398053919688804 2.1629457974375 6 0.45432488288659184 1.5939544752366566 7 0.3149344616850748 1.289069597046244 8 0.20728943938164704 0.9696730013183115 9 0.16248686949199884 0.8551039659606867 >10 1.017317528105521 11.153427966374691 >50 0.08125676571068258 3.238314411294982 >100 0.042351171691181175 4.619413485295834 >500 0.00381013655088493 1.5178101576510272 >1k 0.0016119808484513169 1.729205046090314 >5k 2.930874269911485E-4 1.2264673847715888 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7533 0.6458073105764771 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6780 0.5812522986470882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4150 0.35578127424563655 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3657 0.3135161734738055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 0.16708860325415556 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1773 0.1520000480090394 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1466 0.12568080675761523 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1371 0.11753641614235366 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1296 0.11110663407767349 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1187 0.10176201747700495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1169 0.10021886978148171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.7146085505813808E-4 0.0 0.0 0.0 0.0 8 1.7146085505813808E-4 0.0 0.0 0.0 0.0 9 1.7146085505813808E-4 0.0 0.0 0.0 0.0 10-11 1.7146085505813808E-4 0.0 0.0 0.0 0.0 12-13 1.7146085505813808E-4 0.0 0.0 0.0 0.0 14-15 1.7146085505813808E-4 0.0 0.0 0.0 0.0 16-17 1.7146085505813808E-4 0.0 0.0 0.0 0.0 18-19 2.143260688226726E-4 0.0 0.0 0.0 0.0 20-21 2.5719128258720715E-4 0.0 0.0 0.0 0.0 22-23 2.5719128258720715E-4 0.0 0.0 0.0 0.0 24-25 2.5719128258720715E-4 0.0 0.0 0.0 0.0 26-27 2.5719128258720715E-4 0.0 0.0 5.143825651744143E-4 0.0 28-29 3.0005649635174165E-4 0.0 0.0 0.0015431476955232427 0.0 30-31 3.4292171011627616E-4 0.0 0.0 0.0028291041084592787 0.0 32-33 3.4292171011627616E-4 0.0 0.0 0.004372251803982522 0.0 34-35 3.8578692388081066E-4 0.0 0.0 0.0066869733472673856 0.0 36-37 5.143825651744143E-4 0.0 0.0 0.00985899916584294 0.0 38-39 5.143825651744143E-4 0.0 0.0 0.014745633534999875 8.573042752906904E-5 40-41 5.143825651744143E-4 0.0 0.0 0.02078962867579924 8.573042752906904E-5 42-43 5.143825651744143E-4 0.0 0.0 0.030434301772819514 8.573042752906904E-5 44-45 5.143825651744143E-4 0.0 0.0 0.03935026623584269 8.573042752906904E-5 46-47 5.143825651744143E-4 0.0 0.0 0.05585337353518848 1.2859564129360357E-4 48-49 5.143825651744143E-4 8.573042752906904E-5 0.0 0.07505698930169995 1.7146085505813808E-4 50-51 5.143825651744143E-4 8.573042752906904E-5 0.0 0.08337284077201965 1.7146085505813808E-4 52-53 5.143825651744143E-4 8.573042752906904E-5 0.0 0.095589426694912 2.143260688226726E-4 54-55 5.143825651744143E-4 8.573042752906904E-5 0.0 0.11530742502659785 2.5719128258720715E-4 56-57 5.143825651744143E-4 8.573042752906904E-5 0.0 0.15281448707056555 2.5719128258720715E-4 58-59 5.143825651744143E-4 8.573042752906904E-5 0.0 0.20223807854107387 2.5719128258720715E-4 60-61 5.572477789389488E-4 8.573042752906904E-5 0.0 0.253890661127338 2.5719128258720715E-4 62-63 6.001129927034833E-4 8.573042752906904E-5 0.0 0.3139448256114509 2.5719128258720715E-4 64-65 6.001129927034833E-4 8.573042752906904E-5 0.0 0.3772996115554329 2.5719128258720715E-4 66-67 6.001129927034833E-4 8.573042752906904E-5 0.0 0.46598773883425476 2.5719128258720715E-4 68-69 6.001129927034833E-4 8.573042752906904E-5 0.0 0.5743509992309981 2.5719128258720715E-4 70-71 7.715738477616213E-4 8.573042752906904E-5 0.0 0.6345766245701691 2.5719128258720715E-4 72-73 8.573042752906904E-4 8.573042752906904E-5 0.0 0.7152060916612585 2.5719128258720715E-4 74-75 8.573042752906904E-4 1.7146085505813808E-4 0.0 0.8021796103894991 2.5719128258720715E-4 76-77 8.573042752906904E-4 1.7146085505813808E-4 0.0 0.9027414018810971 2.5719128258720715E-4 78-79 8.573042752906904E-4 1.7146085505813808E-4 0.0 1.009089997230907 2.5719128258720715E-4 80-81 0.0010287651303488286 1.7146085505813808E-4 0.0 1.1678198838009786 2.5719128258720715E-4 82-83 0.0010287651303488286 1.7146085505813808E-4 0.0 1.3394521997141746 3.4292171011627616E-4 84-85 0.0011144955578778976 1.7146085505813808E-4 0.0 1.5903851610917599 3.4292171011627616E-4 86-87 0.0011144955578778976 1.7146085505813808E-4 0.0 1.9507530132102016 3.4292171011627616E-4 88-89 0.0011144955578778976 1.7146085505813808E-4 0.0 2.3283955464757504 3.4292171011627616E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1060 0.0 29.578062 2 GTACACG 195 1.6370905E-11 29.235836 1 GTACCTG 1250 0.0 26.224545 1 GTACATG 7170 0.0 25.311222 1 TACATGG 7330 0.0 24.49737 2 GAGTACT 7585 0.0 24.048536 12-13 GGTATCA 10235 0.0 23.626469 1 ACATGGG 7295 0.0 22.983978 3 GTATCAA 13500 0.0 22.839144 1 GTAAGGT 470 0.0 22.232176 4 GTACTTT 8225 0.0 22.090652 14-15 TAAGGTG 555 0.0 21.3946 5 CATGGGA 4545 0.0 20.900335 4 GCACCGT 345 1.8189894E-12 20.650442 6 AGTACTT 7780 0.0 20.60664 12-13 CCTATAC 280 1.5770638E-9 20.356308 3 ATATAGG 450 0.0 20.057312 2 CTACACG 240 1.4144825E-7 19.790005 4 TCAACGC 15580 0.0 19.754438 4 ATCAACG 15575 0.0 19.700636 3 >>END_MODULE