FastQCFastQC Report
Thu 26 May 2016
SRR936478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266190
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.5108238100127154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.4169200514930619No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33560.2650471098334373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30620.24182784574195026No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20240.15984962762302657No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14750.11649120590116807No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.11056792424517646No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13430.10606623018662287No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13010.10274919245926756No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13000.102670215370521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15000.035.1744542
GTACCTG16150.033.84841
TATCACG700.00836346627.161742
GTACATG81700.026.7056661
TACATGG82900.025.687342
ACATGGG83200.024.5653863
GTATCAA120550.024.013911
GAGTACT59200.022.83684212-13
GGTATCA90350.021.8865831
CATGGGA48950.021.4594234
GTACACG2709.931682E-1021.1266331
ATCAACG139700.020.6864343
TCAACGC139450.020.655354
ACCTGGG25350.020.6249623
GTACTTT65450.020.61612114-15
CATGGGT10150.020.6046434
CAACGCA140100.020.5280285
TATCAAC141150.020.5084132
AGTACTT60600.020.38807312-13
AACGCAG143650.020.0506426
Produced by FastQC (version 0.11.5)