##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936478_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1266190 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.420143896255695 31.0 31.0 34.0 30.0 34.0 2 31.44586673406045 31.0 31.0 34.0 28.0 34.0 3 31.48469345043003 31.0 31.0 34.0 28.0 34.0 4 35.14394601126213 37.0 35.0 37.0 32.0 37.0 5 35.08195610453407 37.0 35.0 37.0 32.0 37.0 6 35.210019823249276 37.0 35.0 37.0 32.0 37.0 7 35.054088248998966 37.0 35.0 37.0 32.0 37.0 8 35.040916450137814 37.0 35.0 37.0 32.0 37.0 9 36.54535891137981 39.0 37.0 39.0 32.0 39.0 10-11 36.42122864656963 39.0 37.0 39.0 32.0 39.0 12-13 36.33334847060868 39.0 36.0 39.0 32.0 39.0 14-15 37.39007021063189 40.0 37.0 41.0 32.0 41.0 16-17 37.33724204108388 40.0 37.0 41.0 32.0 41.0 18-19 37.32756537328521 40.0 37.0 41.0 32.0 41.0 20-21 37.24925129719868 39.5 37.0 41.0 31.0 41.0 22-23 37.1429931526864 39.0 36.0 41.0 31.0 41.0 24-25 37.00365624432352 39.0 36.0 41.0 31.0 41.0 26-27 36.796488283748886 39.0 36.0 41.0 30.5 41.0 28-29 36.889013497184465 39.0 36.0 41.0 30.0 41.0 30-31 36.78666471856515 39.0 36.0 41.0 30.0 41.0 32-33 36.601219406250245 39.0 35.0 41.0 30.0 41.0 34-35 36.4135532581998 39.0 35.0 40.5 30.0 41.0 36-37 36.15501188605185 39.0 35.0 40.0 29.5 41.0 38-39 35.96471066743538 38.0 35.0 40.0 29.0 41.0 40-41 35.80467978739368 38.0 35.0 40.0 28.5 41.0 42-43 35.51120605912225 38.0 35.0 40.0 27.0 41.0 44-45 35.193529012233554 38.0 34.5 40.0 26.5 41.0 46-47 34.79247822206778 38.0 34.0 40.0 24.5 41.0 48-49 34.471214825579096 38.0 33.5 40.0 24.0 41.0 50-51 33.72572007360665 37.5 33.0 39.5 22.5 40.5 52-53 33.88985539295051 37.5 33.0 39.5 23.0 40.5 54-55 34.23690678334215 38.0 34.0 40.0 23.0 41.0 56-57 34.151132926338065 38.0 34.0 40.0 23.0 41.0 58-59 33.918730996138024 38.0 33.0 40.0 22.0 41.0 60-61 33.592287492398455 37.0 33.0 40.0 21.5 41.0 62-63 33.27684312780862 37.0 33.0 40.0 20.0 41.0 64-65 32.95379129514528 36.0 32.0 39.0 19.5 41.0 66-67 32.58475900141369 36.0 32.0 39.0 18.0 41.0 68-69 32.2094168331767 35.0 31.5 39.0 17.0 40.5 70-71 31.813566684304885 35.0 31.0 38.0 13.5 40.0 72-73 31.411232121561532 35.0 31.0 37.0 10.0 40.0 74-75 30.98943168086938 35.0 31.0 37.0 7.5 39.0 76-77 30.58100364084379 35.0 30.0 36.0 7.0 39.0 78-79 30.142796499735425 34.0 30.0 36.0 3.5 38.5 80-81 29.745605714782144 34.0 29.5 35.5 2.0 37.0 82-83 29.34798489958063 34.0 29.0 35.0 2.0 37.0 84-85 28.974219508920463 34.0 29.0 35.0 2.0 36.5 86-87 28.645919253824466 34.0 29.0 35.0 2.0 36.0 88-89 28.272708282327297 34.0 28.5 35.0 2.0 36.0 90-91 27.93331687977318 34.0 27.0 35.0 2.0 35.0 92-93 27.58157780427898 34.0 27.0 35.0 2.0 35.0 94-95 27.175076805218808 33.5 26.0 35.0 2.0 35.0 96-97 26.60379761331238 33.0 25.0 35.0 2.0 35.0 98-99 25.98101746183432 33.0 24.5 35.0 2.0 35.0 100-101 23.95450366848577 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3262109520005865 1101 2 0.3880481699432252 1101 3 0.3881875710465117 1101 4 0.33697510485097126 1101 5 0.4564980322548493 1101 6 0.1515510681567065 1101 7 0.3530855904460424 1101 8 0.2759723394921494 1101 9 0.6377988679421591 1101 10-11 0.5201917543358903 1101 12-13 0.6339220040155595 1101 14-15 0.4655876702131465 1101 16-17 0.6554130230415538 1101 18-19 0.7776292815596548 1101 20-21 0.8133536488748021 1101 22-23 0.6032910304179566 1101 24-25 0.6950948659087786 1101 26-27 0.749183024198949 1101 28-29 0.7976280636693502 1101 30-31 0.675499411933437 1101 32-33 0.6362085182443096 1101 34-35 0.49442740792482454 1101 36-37 0.6416914533161346 1101 38-39 0.6115137969480671 1101 40-41 0.5968393044142246 1101 42-43 0.447099464920683 1101 44-45 0.6719259549799901 1101 46-47 0.7252813832417573 1101 48-49 0.5358910536793715 1101 50-51 0.5216515850444949 1101 52-53 0.5806696456660987 1101 54-55 0.5100391305815322 1101 56-57 0.40159321899263745 1101 58-59 0.3908246498964658 1101 60-61 0.541796224121228 1101 62-63 0.6660020691941853 1101 64-65 0.6636999411828626 1101 66-67 0.6933439547826623 1101 68-69 0.3945485921203371 1101 70-71 0.5241891914115797 1101 72-73 0.6124764510623422 1101 74-75 0.40871929072975277 1101 76-77 0.4704821023263399 1101 78-79 0.2482252068245323 1101 80-81 0.2709009394585884 1101 82-83 0.3288256379678067 1101 84-85 0.24217777774716254 1101 86-87 0.4282244323774371 1101 88-89 0.37919285240197453 1101 90-91 0.3325722419771502 1101 92-93 0.08301197654484582 1101 94-95 0.2150134596528055 1101 96-97 0.2525903581479412 1101 98-99 0.366293238029229 1101 100-101 0.5442118660452095 1102 1 0.32754041869939243 1102 2 0.4338398452312511 1102 3 0.4870864547851035 1102 4 0.3439274744267138 1102 5 0.28308875275860856 1102 6 0.4673727351042345 1102 7 0.4316094504536778 1102 8 0.5101248617992411 1102 9 0.5981351115614686 1102 10-11 0.7290404137769713 1102 12-13 0.7488395482831152 1102 14-15 0.6954571552994366 1102 16-17 0.7044777034895944 1102 18-19 0.7245722014162226 1102 20-21 0.5497587453237927 1102 22-23 0.20887991003962725 1102 24-25 0.34954631291035554 1102 26-27 0.30951404167559105 1102 28-29 0.49362441176884886 1102 30-31 0.49304917227745904 1102 32-33 0.30478841529996004 1102 34-35 0.3631096413862167 1102 36-37 0.45780855730783543 1102 38-39 0.33802495209624794 1102 40-41 0.3706427843852964 1102 42-43 0.4464275322931286 1102 44-45 0.5164922064705095 1102 46-47 0.822810004372009 1102 48-49 0.6272264291190695 1102 50-51 0.6608258971668022 1102 52-53 0.5188571767296537 1102 54-55 0.42196716126432676 1102 56-57 0.6689632106947982 1102 58-59 0.493364768540566 1102 60-61 0.1205643263061873 1102 62-63 0.3783941066858958 1102 64-65 0.5442572506366403 1102 66-67 0.45197722248701666 1102 68-69 0.7403213893012079 1102 70-71 0.7866818054854718 1102 72-73 0.44206287119728316 1102 74-75 0.5721690355020108 1102 76-77 0.6688115498270726 1102 78-79 0.5384166436298052 1102 80-81 0.6600189905553293 1102 82-83 0.6513421003171835 1102 84-85 0.44697297694852267 1102 86-87 0.5053534989030304 1102 88-89 0.36481429409208843 1102 90-91 0.24500875500944375 1102 92-93 0.42567148588470616 1102 94-95 0.5557310303710494 1102 96-97 0.5128231988017831 1102 98-99 0.38040462312768497 1102 100-101 0.6666011944752128 1103 1 0.20469301587377942 1103 2 0.4501810074077959 1103 3 0.4162282274708424 1103 4 0.4281812574750461 1103 5 0.3818180300884322 1103 6 0.2536940155669356 1103 7 0.31229557040668254 1103 8 0.21346760045511814 1103 9 0.568668503803579 1103 10-11 0.4124901421606282 1103 12-13 0.6464366724635013 1103 14-15 0.5590793926951321 1103 16-17 0.7421076124152819 1103 18-19 0.609524354766485 1103 20-21 0.5876816082905947 1103 22-23 0.5797637425914104 1103 24-25 0.5601631983093469 1103 26-27 0.5315375583290418 1103 28-29 0.5995417319548082 1103 30-31 0.4987739511185012 1103 32-33 0.5920154182822088 1103 34-35 0.657406953338338 1103 36-37 0.6786577474794413 1103 38-39 0.39697172332600417 1103 40-41 0.4483657256739946 1103 42-43 0.5690254095687308 1103 44-45 0.5469832747611818 1103 46-47 0.3927652705158593 1103 48-49 0.48703474128205215 1103 50-51 -0.055443971995458696 1103 52-53 0.30350909611834 1103 54-55 0.6003209453167813 1103 56-57 0.6150069504652578 1103 58-59 0.5949372313627634 1103 60-61 0.6652585194399663 1103 62-63 0.6360314963574467 1103 64-65 0.6362008889902668 1103 66-67 0.34762533623452896 1103 68-69 0.18342404120051015 1103 70-71 0.3260450885789865 1103 72-73 0.39699740211937495 1103 74-75 0.34629218163260944 1103 76-77 0.22325782401398087 1103 78-79 0.4391659733750579 1103 80-81 0.606592658329884 1103 82-83 0.5969269540660918 1103 84-85 0.41278672143837625 1103 86-87 0.47132890783373327 1103 88-89 0.5750224998176172 1103 90-91 0.6822247125951186 1103 92-93 0.6592499935512173 1103 94-95 0.7774947291731245 1103 96-97 0.9251219968618116 1103 98-99 0.8014523794059869 1103 100-101 0.7668527282791473 1104 1 0.4381823810704617 1104 2 0.40648270029485545 1104 3 0.3152673996807458 1104 4 0.34356506066372106 1104 5 0.4207579519551885 1104 6 0.44774368766538686 1104 7 0.586679361863645 1104 8 0.5350950245215671 1104 9 0.6373412944275927 1104 10-11 0.7184205261397949 1104 12-13 0.5444915534452122 1104 14-15 0.6604531769957447 1104 16-17 0.5142834943472607 1104 18-19 0.5103221116618073 1104 20-21 0.590903138341389 1104 22-23 0.651342480023807 1104 24-25 0.6789010999740768 1104 26-27 0.6398752910570167 1104 28-29 0.5892424558018732 1104 30-31 0.5999459498514739 1104 32-33 0.5016014059169791 1104 34-35 0.5226728527505813 1104 36-37 0.5036944091014703 1104 38-39 0.5561723239900118 1104 40-41 0.5225970047833215 1104 42-43 0.6141761034999149 1104 44-45 0.6764134651669806 1104 46-47 0.7187756648189421 1104 48-49 0.4753605076566032 1104 50-51 0.4061416871511767 1104 52-53 0.490841185889451 1104 54-55 0.7287363420248596 1104 56-57 0.68906577330457 1104 58-59 0.6346983735629337 1104 60-61 0.47493366813950644 1104 62-63 0.29021209444048424 1104 64-65 0.48632465862623775 1104 66-67 0.36036950263722645 1104 68-69 0.4985157245848768 1104 70-71 0.5939412394019641 1104 72-73 0.14484404508796445 1104 74-75 0.0560729158035862 1104 76-77 0.23181963257578886 1104 78-79 0.38771534631467475 1104 80-81 0.5044680440101494 1104 82-83 0.41355549264584823 1104 84-85 0.4528751786000065 1104 86-87 0.415482405645772 1104 88-89 0.11667948781607151 1104 90-91 0.39714393970946915 1104 92-93 0.21250378582939788 1104 94-95 0.15663221318865794 1104 96-97 0.4098062376436076 1104 98-99 0.45474723270084283 1104 100-101 0.8190764756248434 1105 1 0.14814072153727764 1105 2 0.22488365903479846 1105 3 0.22277638786770027 1105 4 0.36084292404328266 1105 5 0.30240150614111627 1105 6 0.31696927689097265 1105 7 0.5506467190513433 1105 8 0.2573275189574531 1105 9 0.4496063175400735 1105 10-11 0.39624966498588066 1105 12-13 0.3923954511796097 1105 14-15 0.39496553218607033 1105 16-17 0.40640927189677 1105 18-19 0.2596584125235353 1105 20-21 0.05454749147727256 1105 22-23 -0.051805382785076404 1105 24-25 -0.1304397663159378 1105 26-27 -0.01252669490428815 1105 28-29 -0.022109481651753526 1105 30-31 0.059514516877662516 1105 32-33 -0.11938244388042563 1105 34-35 -0.11402634259268041 1105 36-37 -0.06707543624464307 1105 38-39 -0.04443145390189329 1105 40-41 -0.04052460681286618 1105 42-43 -0.022541353556526644 1105 44-45 0.06614467557616166 1105 46-47 -0.02269212037766266 1105 48-49 0.15878457887728104 1105 50-51 0.09585349449010039 1105 52-53 0.21676368774416233 1105 54-55 0.08883449873318483 1105 56-57 0.20082517523346155 1105 58-59 0.14838552758906332 1105 60-61 0.27040704598575616 1105 62-63 0.09722915772874074 1105 64-65 -0.06444518671988675 1105 66-67 -0.11505936228316216 1105 68-69 0.049613139411107454 1105 70-71 0.129340429038443 1105 72-73 -0.07077086103541319 1105 74-75 0.00440710515472631 1105 76-77 0.16296726711325604 1105 78-79 0.08938743442744013 1105 80-81 -0.0109151666272993 1105 82-83 0.05893014310524691 1105 84-85 -0.01438271103076616 1105 86-87 -0.009176613928506328 1105 88-89 0.004777416252981226 1105 90-91 -0.03314881837989958 1105 92-93 0.010548641162387185 1105 94-95 0.047539021383599334 1105 96-97 0.22668462604742246 1105 98-99 0.32736036972075766 1105 100-101 0.6906098888531744 1106 1 0.18033602383760083 1106 2 0.07768552609890733 1106 3 0.1152921874355961 1106 4 0.20523699472829549 1106 5 0.3975566134164268 1106 6 0.32504016986984396 1106 7 0.36686568315426626 1106 8 0.46115315180669114 1106 9 0.5337036914034883 1106 10-11 0.4712742164167949 1106 12-13 0.35716016181933696 1106 14-15 0.41187124313773893 1106 16-17 0.21005305085301273 1106 18-19 0.20356538281919256 1106 20-21 0.2897277856322944 1106 22-23 0.24114645019587044 1106 24-25 0.11632441285751582 1106 26-27 0.20679603221088172 1106 28-29 0.04402132837216044 1106 30-31 0.0938481621585936 1106 32-33 0.07521277523387226 1106 34-35 0.06695617678850851 1106 36-37 0.0906106058336178 1106 38-39 0.08977625698045699 1106 40-41 0.24734964364638756 1106 42-43 0.2109826144168494 1106 44-45 0.05476136130629072 1106 46-47 0.18892087974942484 1106 48-49 0.20732431753452119 1106 50-51 0.2125389935484776 1106 52-53 0.19453869265444723 1106 54-55 0.5419337558357711 1106 56-57 0.5269125436666755 1106 58-59 0.40486837624826677 1106 60-61 0.402392303573599 1106 62-63 0.4620800583329867 1106 64-65 0.41093683354515775 1106 66-67 0.32569763054204515 1106 68-69 0.2635897780024905 1106 70-71 0.2263099159424229 1106 72-73 0.46477753127911825 1106 74-75 0.31546437642203884 1106 76-77 0.04899548103998441 1106 78-79 0.2605438357075833 1106 80-81 0.4200492135790448 1106 82-83 0.4325532410914512 1106 84-85 0.4561678187024576 1106 86-87 0.30589161016540345 1106 88-89 0.10522754507578469 1106 90-91 0.25677088399898906 1106 92-93 0.08483445447908622 1106 94-95 0.08611931322790412 1106 96-97 0.2787988219736164 1106 98-99 0.4028643961554259 1106 100-101 0.6619675540987444 1107 1 0.44407799278002713 1107 2 0.4129392927979616 1107 3 0.4256318414405378 1107 4 0.3435851955675773 1107 5 0.4392971646804469 1107 6 0.5172018350305123 1107 7 0.5737533128910854 1107 8 0.5357024274545523 1107 9 0.6287487242071137 1107 10-11 0.6639547722554369 1107 12-13 0.6634673020721209 1107 14-15 0.6532694907422965 1107 16-17 0.669668182871483 1107 18-19 0.8694303591421431 1107 20-21 0.891400917908932 1107 22-23 0.9023632077887243 1107 24-25 0.8602503063232874 1107 26-27 0.9759788515791783 1107 28-29 0.8991183464917256 1107 30-31 0.7652434430559438 1107 32-33 0.8067690849218749 1107 34-35 0.7367177871443502 1107 36-37 0.8677449365240975 1107 38-39 0.8232195962698512 1107 40-41 0.6321210939585669 1107 42-43 0.8381405542085503 1107 44-45 0.7114918235011558 1107 46-47 0.7473171706860242 1107 48-49 0.8668216305130798 1107 50-51 0.7156766827326777 1107 52-53 0.7690163483669465 1107 54-55 1.080869523227804 1107 56-57 0.9082915303210228 1107 58-59 0.8326205633455857 1107 60-61 0.8745041235239128 1107 62-63 1.0471387810183401 1107 64-65 0.8340130598030058 1107 66-67 0.7891171116174007 1107 68-69 0.8926271041613774 1107 70-71 0.8155840238354486 1107 72-73 0.7558500072567185 1107 74-75 0.777195268902684 1107 76-77 0.789287724346206 1107 78-79 0.8595208878876583 1107 80-81 0.8959999186815573 1107 82-83 0.8497812608483635 1107 84-85 0.9442610752184599 1107 86-87 0.7627376267382004 1107 88-89 0.8390955084879046 1107 90-91 0.820890541944447 1107 92-93 0.7187447670703797 1107 94-95 0.7619145492849455 1107 96-97 0.8392638816361391 1107 98-99 0.7606716485089784 1107 100-101 0.968645532752042 1108 1 -0.008475528367718255 1108 2 -0.18792777214252254 1108 3 -0.37504378390397974 1108 4 -0.17143697765669685 1108 5 0.07399888142929711 1108 6 0.020078394977673497 1108 7 -0.005979664876093693 1108 8 -0.14741165663982514 1108 9 0.18146413720514687 1108 10-11 -0.10876160911409016 1108 12-13 0.09563225794914132 1108 14-15 0.19192861900931035 1108 16-17 0.07027540146024336 1108 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62-63 -2.160510153176169 2316 64-65 -2.259639568083333 2316 66-67 -2.6659137379317244 2316 68-69 -2.0818755887256906 2316 70-71 -1.6132866144097378 2316 72-73 -1.8021347640026448 2316 74-75 -2.037106437624214 2316 76-77 -2.7217288663143258 2316 78-79 -2.6799025660237348 2316 80-81 -2.834316727693878 2316 82-83 -2.457865609831156 2316 84-85 -2.3888753643927743 2316 86-87 -2.3732660669536365 2316 88-89 -2.673638958402993 2316 90-91 -2.826998998098066 2316 92-93 -3.0078523935329926 2316 94-95 -3.002610016861027 2316 96-97 -3.377449042158254 2316 98-99 -4.024900770549646 2316 100-101 -4.5953672816760545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2568.0 3 1028.0 4 1012.0 5 1122.0 6 1373.0 7 1576.0 8 1846.0 9 2546.0 10 3123.0 11 4363.0 12 5575.0 13 7083.0 14 7751.0 15 9504.0 16 12025.0 17 14769.0 18 13195.0 19 8348.0 20 8336.0 21 8722.0 22 9735.0 23 10646.0 24 12477.0 25 14038.0 26 16846.0 27 19571.0 28 23764.0 29 28589.0 30 34975.0 31 42305.0 32 53750.0 33 68789.0 34 90887.0 35 124737.0 36 172961.0 37 223775.0 38 174419.0 39 28048.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.87596415495989 18.29542537351748 13.95594804127946 21.872662430243167 2 15.600989485922256 20.40435601273821 40.12923027346712 23.865424227872413 3 19.771073279613802 25.72440015733578 29.477395618925158 25.027130944125265 4 12.547223622318466 16.368564670489324 37.31752444726044 33.76668725993177 5 13.348045187990957 37.883578820618084 33.46939033505419 15.298985656336765 6 30.942393979096895 35.879973890875874 17.887910938174162 15.289721191853067 7 28.579870110890006 33.49506931711444 20.3233561032951 17.60170446870045 8 27.176373934544074 33.21749915079509 19.734337106699634 19.871789807961196 9 27.500077013548857 16.797642358557706 20.501954564375822 35.200326063517615 10-11 25.20347590012555 26.62956522013955 26.579274021059884 21.587684858675015 12-13 26.843713519887647 24.286100867325473 27.238602403070306 21.631583209716574 14-15 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26.324165678080558 29.744581468709868 21.830271744023857 40-41 22.052793798535326 26.369019529638017 29.847707918279987 21.73047875354667 42-43 21.81710700585934 26.61680815490336 29.23996768239662 22.326117156840677 44-45 22.05036124633936 26.617671216864693 29.108407072656796 22.22356046413915 46-47 22.53591095859306 26.98600681987359 28.46643670489929 22.011645516634054 48-49 22.51712927185342 27.07169317486725 28.091729417725197 22.319448135554133 50-51 22.309625155933283 27.42139363188017 28.1895390535131 22.079442158673444 52-53 22.66950775600013 27.4950816668391 27.78911545821648 22.04629511894429 54-55 22.006069545060548 27.325431472782807 28.058199710541544 22.6102992716151 56-57 22.485869771677308 27.625075177592873 27.558140958332167 22.330914092397656 58-59 22.739742472389846 27.097501057334583 27.64790738578318 22.514849084492386 60-61 22.35455836383194 27.308759116016862 28.31967543268008 22.017007087471114 62-63 22.35719159798195 27.36342297886859 28.24553062301172 22.03385480013774 64-65 22.006480287644212 27.63232561767093 28.06809633158854 22.293097763096323 66-67 22.0046439628483 27.716244392493838 27.674543501611172 22.60456814304669 68-69 22.128585171366332 27.52026272432743 28.02219066469318 22.328961439613053 70-71 22.302711446460208 27.544730691735182 27.65388676992 22.498671091884617 72-73 22.199524385322132 27.45825734845212 27.83230436180203 22.509913904423723 74-75 22.059063372108405 28.255532427673373 27.40835691863948 22.277047281578742 76-77 22.290596332992408 27.715129664219624 27.517207608863053 22.477066393924915 78-79 22.269838661511503 27.755560576590355 27.594079883961424 22.380520877936714 80-81 22.319059127606824 28.092238798070827 27.234767441998244 22.3539346323241 82-83 22.358805219955755 27.955101367067332 27.375703022017294 22.31039039095962 84-85 22.180637303686012 27.95430634934175 27.28831578315499 22.576740563817253 86-87 22.453299465523628 27.99330858013473 27.127736456627797 22.425655497713844 88-89 22.311295365269874 27.91824349166019 26.944564342160394 22.825896800909547 90-91 22.115032972969985 27.78370616340597 27.395091928592922 22.706168935031123 92-93 22.284578445818738 27.87934031240336 27.459166437273876 22.376914804504025 94-95 21.97548327936906 27.67429195835609 27.559649968742978 22.79057479353187 96-97 21.92395299722463 27.653344264211327 27.59758414959507 22.825118588968976 98-99 21.857847092786503 27.850801576571506 27.06721599416705 23.224135336474944 100-101 21.777480704033174 27.755694769368855 27.482600248562232 22.984224278035743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 333.0 1 255.0 2 344.5 3 490.0 4 437.5 5 423.0 6 587.0 7 1040.5 8 1661.0 9 2055.0 10 1898.5 11 1699.5 12 1764.5 13 1858.0 14 1994.0 15 2098.5 16 2183.5 17 2294.5 18 2399.5 19 2596.0 20 2880.5 21 3243.0 22 3683.5 23 4300.5 24 5186.5 25 6234.5 26 7703.5 27 9420.5 28 11022.0 29 12932.5 30 15451.0 31 18391.0 32 21859.5 33 24060.5 34 25530.0 35 27492.5 36 30113.5 37 33020.5 38 34228.0 39 34183.5 40 34513.5 41 36195.0 42 37679.0 43 38964.0 44 40567.0 45 42757.5 46 43919.5 47 46356.0 48 54807.5 49 54111.0 50 45840.5 51 42387.0 52 40439.5 53 40296.0 54 40084.0 55 37685.0 56 34326.0 57 31290.5 58 29528.5 59 26866.0 60 22588.5 61 19181.0 62 16411.0 63 12720.0 64 9079.5 65 6945.0 66 5131.5 67 3941.0 68 2971.5 69 2048.0 70 1541.5 71 1261.0 72 1080.5 73 985.0 74 776.0 75 568.5 76 425.5 77 253.5 78 160.5 79 108.5 80 63.5 81 44.5 82 35.5 83 17.5 84 12.5 85 11.5 86 9.5 87 5.5 88 2.5 89 3.0 90 2.5 91 2.5 92 2.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014610761418112605 2 0.00616021292223126 3 0.0085295255846279 4 0.03190674385360807 5 0.04296353627812572 6 0.057811228962477985 7 0.026931187262575127 8 0.023693126623966387 9 0.014136898885633279 10-11 0.044306146786817144 12-13 0.04087064342634202 14-15 0.019546829464772267 16-17 0.021679210860929245 18-19 0.028905614481238993 20-21 0.021679210860929245 22-23 0.013820990530647058 24-25 0.0034355033604751262 26-27 0.002566755384263025 28-29 0.0016190303193043699 30-31 0.00675254108783042 32-33 0.0012241448755715967 34-35 0.001658518863677647 36-37 0.005686350389751933 38-39 0.005686350389751933 40-41 0.0029616408279957984 42-43 5.528396212258823E-4 44-45 0.0014215875974379832 46-47 0.0029616408279957984 48-49 7.502823430922689E-4 50-51 0.0018559615855440338 52-53 7.107937987189916E-4 54-55 0.002566755384263025 56-57 0.0016980074080509245 58-59 0.0027247095617561347 60-61 9.477250649586555E-4 62-63 0.0014215875974379832 64-65 0.0033565262717285714 66-67 0.0023693126623966386 68-69 0.005686350389751933 70-71 0.008924411028360672 72-73 0.00308010646111563 74-75 0.003277549182982017 76-77 0.002764198106129412 78-79 0.03190674385360807 80-81 0.02041557744098437 82-83 0.0038698773485811764 84-85 0.00837157140713479 86-87 0.007186915075936471 88-89 0.005094022224152774 90-91 0.012083494578222859 92-93 0.013149685276301346 94-95 0.00588379311161832 96-97 0.004106808614820841 98-99 0.021995119215915463 100-101 0.04035729234948941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1266190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.07815663974869 #Duplication Level Percentage of deduplicated Percentage of total 1 81.54545962143035 48.99100896396595 2 11.072414949403223 13.304205594210838 3 3.201004556687178 5.769313594836048 4 1.2945506874468116 3.1109687591409565 5 0.7064591242848743 2.1221380964183187 6 0.4302267283297262 1.5508337265119936 7 0.30054305883379423 1.2639251075924127 8 0.21284801926916835 1.0230013313690676 9 0.1604906409560726 0.8677783679915337 >10 0.956333201663929 10.849486720976017 >50 0.07525049757592447 3.131401248583217 >100 0.03967388252827057 4.448569755105027 >500 0.0034269672599015486 1.428689604576564 >1k 0.0010544514645850916 1.2084709043034554 >5k 2.636128661462729E-4 0.9302082244186285 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6468 0.5108238100127154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5279 0.4169200514930619 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3356 0.2650471098334373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3062 0.24182784574195026 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2024 0.15984962762302657 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1475 0.11649120590116807 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.11056792424517646 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1343 0.10606623018662287 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1301 0.10274919245926756 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1300 0.102670215370521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.897708874655462E-5 0.0 0.0 0.0 0.0 6 7.897708874655462E-5 0.0 0.0 0.0 0.0 7 1.5795417749310924E-4 0.0 0.0 0.0 0.0 8 1.5795417749310924E-4 0.0 0.0 0.0 0.0 9 1.5795417749310924E-4 0.0 0.0 0.0 0.0 10-11 1.5795417749310924E-4 0.0 0.0 0.0 0.0 12-13 2.3693126623966387E-4 0.0 0.0 0.0 0.0 14-15 2.3693126623966387E-4 0.0 0.0 3.948854437327731E-5 0.0 16-17 2.3693126623966387E-4 0.0 0.0 1.5795417749310924E-4 0.0 18-19 3.159083549862185E-4 0.0 0.0 1.5795417749310924E-4 0.0 20-21 3.948854437327731E-4 0.0 0.0 2.7641981061294116E-4 0.0 22-23 3.948854437327731E-4 0.0 0.0 5.13351076852605E-4 3.948854437327731E-5 24-25 4.7386253247932774E-4 0.0 0.0 6.31816709972437E-4 7.897708874655462E-5 26-27 4.7386253247932774E-4 0.0 0.0 0.0010661906980784874 7.897708874655462E-5 28-29 5.923281655991596E-4 0.0 0.0 0.0016980074080509243 7.897708874655462E-5 30-31 7.107937987189916E-4 0.0 0.0 0.00264573247300958 7.897708874655462E-5 32-33 7.107937987189916E-4 0.0 0.0 0.004541182602926891 7.897708874655462E-5 34-35 7.107937987189916E-4 0.0 0.0 0.006120724377857984 1.5795417749310924E-4 36-37 7.107937987189916E-4 0.0 0.0 0.009635204827079663 1.5795417749310924E-4 38-39 7.107937987189916E-4 0.0 0.0 0.01468973850685916 1.5795417749310924E-4 40-41 7.107937987189916E-4 0.0 0.0 0.020534043074104204 1.5795417749310924E-4 42-43 7.897708874655462E-4 0.0 0.0 0.02953743119121143 1.5795417749310924E-4 44-45 0.00102670215370521 0.0 0.0 0.03715872025525395 2.3693126623966385E-4 46-47 0.00102670215370521 0.0 0.0 0.05149306186275361 3.159083549862185E-4 48-49 0.0011056792424517646 0.0 0.0 0.0704870517063 3.159083549862185E-4 50-51 0.0011056792424517646 0.0 0.0 0.07945095127903395 3.159083549862185E-4 52-53 0.0011056792424517646 0.0 0.0 0.09102109478040421 3.159083549862185E-4 54-55 0.0011056792424517646 0.0 0.0 0.109501733547098 3.159083549862185E-4 56-57 0.0011846563311983193 0.0 0.0 0.13982893562577497 3.159083549862185E-4 58-59 0.0011846563311983193 0.0 0.0 0.17951492272091865 3.159083549862185E-4 60-61 0.0011846563311983193 0.0 0.0 0.22378158096336254 3.159083549862185E-4 62-63 0.0011846563311983193 0.0 0.0 0.27476129174926356 3.159083549862185E-4 64-65 0.0011846563311983193 0.0 0.0 0.3285841777300405 3.159083549862185E-4 66-67 0.001263633419944874 0.0 0.0 0.4064950757785166 3.159083549862185E-4 68-69 0.001263633419944874 0.0 0.0 0.5027681469605667 3.159083549862185E-4 70-71 0.001263633419944874 0.0 0.0 0.553510926480228 3.159083549862185E-4 72-73 0.0013426105086914285 0.0 0.0 0.6240374667309013 3.159083549862185E-4 74-75 0.0014215875974379832 0.0 0.0 0.7006847313594327 3.159083549862185E-4 76-77 0.0014215875974379832 0.0 0.0 0.785901010116965 3.159083549862185E-4 78-79 0.0014215875974379832 0.0 0.0 0.8755400058443046 3.159083549862185E-4 80-81 0.0018164730411707563 0.0 0.0 1.0071553242404379 3.159083549862185E-4 82-83 0.0018164730411707563 0.0 0.0 1.1534998696878036 3.159083549862185E-4 84-85 0.0018164730411707563 0.0 0.0 1.3837180833840104 3.159083549862185E-4 86-87 0.0018164730411707563 0.0 0.0 1.7151849248533 3.159083549862185E-4 88-89 0.0018164730411707563 0.0 0.0 2.0614204819181956 3.159083549862185E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1500 0.0 35.174454 2 GTACCTG 1615 0.0 33.8484 1 TATCACG 70 0.008363466 27.16174 2 GTACATG 8170 0.0 26.705666 1 TACATGG 8290 0.0 25.68734 2 ACATGGG 8320 0.0 24.565386 3 GTATCAA 12055 0.0 24.01391 1 GAGTACT 5920 0.0 22.836842 12-13 GGTATCA 9035 0.0 21.886583 1 CATGGGA 4895 0.0 21.459423 4 GTACACG 270 9.931682E-10 21.126633 1 ATCAACG 13970 0.0 20.686434 3 TCAACGC 13945 0.0 20.65535 4 ACCTGGG 2535 0.0 20.624962 3 GTACTTT 6545 0.0 20.616121 14-15 CATGGGT 1015 0.0 20.604643 4 CAACGCA 14010 0.0 20.528028 5 TATCAAC 14115 0.0 20.508413 2 AGTACTT 6060 0.0 20.388073 12-13 AACGCAG 14365 0.0 20.050642 6 >>END_MODULE