FastQCFastQC Report
Thu 26 May 2016
SRR936478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266190
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80180.633238297569875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70930.560184490479312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45770.3614781351929805No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38970.3077737148453234No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.15708542951689716No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17460.13789399695148435No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14890.11759688514361984No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14150.11175258057637479No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.104091802967959No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.10124862777308302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12500.031.9245532
TATCACG902.4108034E-531.6711852
GTACACG2201.8189894E-1228.3861681
GTACCTG15000.027.2216051
ATCACGC1109.475818E-525.9097163
GTACATG76950.025.2832261
TACATGG78650.024.5235212
ACATGGG76650.024.4787373
GTATCAA137600.024.0190641
GGTATCA102900.023.9957221
CCTATAC2853.6379788E-1223.3338993
TCACGCA1652.4305318E-623.0299474
TAAGGTG5650.022.6942845
CATGGGG29350.022.4953964
GAGTACT86450.022.05574412-13
GTACTTT90850.020.90914314-15
AGTACTT88050.020.8459312-13
GGACCGT1150.003728079120.6477786
CATGGGA45700.020.5795524
ATCAACG166150.019.9267523
Produced by FastQC (version 0.11.5)