##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936478_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1266190 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.496371792542984 31.0 31.0 34.0 30.0 34.0 2 31.7154242254322 31.0 31.0 34.0 30.0 34.0 3 31.984551291670286 33.0 31.0 34.0 30.0 34.0 4 35.80088059453952 37.0 35.0 37.0 35.0 37.0 5 35.62996390747044 37.0 35.0 37.0 33.0 37.0 6 35.74596229633783 37.0 35.0 37.0 33.0 37.0 7 35.68340296479991 37.0 35.0 37.0 33.0 37.0 8 35.66496181457759 37.0 35.0 37.0 33.0 37.0 9 37.29480251778959 39.0 37.0 39.0 34.0 39.0 10-11 37.183015977065054 39.0 37.0 39.0 33.0 39.0 12-13 37.05685126244876 39.0 37.0 39.0 33.0 39.0 14-15 38.15047544207425 40.0 37.0 41.0 33.0 41.0 16-17 38.07777505745583 40.0 37.0 41.0 33.0 41.0 18-19 38.17213095980856 40.0 37.5 41.0 33.5 41.0 20-21 38.25936549806901 40.0 38.0 41.0 34.0 41.0 22-23 38.1736184932751 40.0 37.5 41.0 33.0 41.0 24-25 38.05445391291986 40.0 37.0 41.0 33.0 41.0 26-27 37.83641120211027 40.0 37.0 41.0 33.0 41.0 28-29 37.73896295184767 40.0 37.0 41.0 33.0 41.0 30-31 37.669774283480365 39.5 37.0 41.0 33.0 41.0 32-33 37.435984725831034 39.0 36.0 41.0 32.5 41.0 34-35 37.23744224800386 39.0 36.0 41.0 32.0 41.0 36-37 36.97028052661923 39.0 35.5 41.0 31.0 41.0 38-39 36.79234593544413 39.0 35.0 41.0 31.0 41.0 40-41 36.53083739407198 39.0 35.0 41.0 30.5 41.0 42-43 36.24281426958039 39.0 35.0 40.0 30.0 41.0 44-45 35.89991391497327 39.0 35.0 40.0 29.5 41.0 46-47 35.382427992639336 38.0 35.0 40.0 27.5 41.0 48-49 34.82994534785459 38.0 34.0 40.0 24.5 41.0 50-51 34.599258405136666 38.0 34.0 40.0 24.0 41.0 52-53 34.316488836588505 38.0 34.0 40.0 23.5 41.0 54-55 34.0366481333765 38.0 33.0 40.0 23.0 41.0 56-57 33.758827664094646 37.5 33.0 40.0 22.0 41.0 58-59 33.389195934259476 37.0 33.0 40.0 21.0 41.0 60-61 33.00710991241441 36.5 32.0 39.5 20.0 41.0 62-63 32.71003285446892 36.0 32.0 39.0 19.0 40.5 64-65 32.272459899383186 35.5 31.0 39.0 18.0 40.0 66-67 31.871880602437233 35.0 31.0 38.5 15.0 40.0 68-69 31.42248240785348 35.0 31.0 38.0 12.5 40.0 70-71 30.942921283535647 35.0 30.0 37.0 8.0 39.5 72-73 30.529949691594467 34.0 30.0 37.0 7.5 39.0 74-75 30.17260798142459 34.0 29.0 36.0 7.0 39.0 76-77 28.773056966174114 32.5 28.0 35.0 3.5 37.0 78-79 29.559728002906358 34.0 29.0 35.0 2.0 37.0 80-81 29.40333362291599 34.0 29.0 35.0 2.0 37.0 82-83 29.06654017169619 34.0 29.0 35.0 2.0 36.5 84-85 28.628108735655786 34.0 29.0 35.0 2.0 36.0 86-87 28.249101240730063 33.5 28.0 35.0 2.0 36.0 88-89 27.84181402475142 33.0 27.0 35.0 2.0 35.0 90-91 27.385384105071118 33.0 26.0 35.0 2.0 35.0 92-93 26.94129119642392 33.0 25.5 35.0 2.0 35.0 94-95 26.42884045838302 32.5 25.0 34.0 2.0 35.0 96-97 25.746042063197468 32.0 23.5 34.0 2.0 35.0 98-99 24.902798553139732 32.0 19.0 34.0 2.0 35.0 100-101 22.12921244047102 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35858011982030646 1101 2 0.38328140315040926 1101 3 0.21431556053997625 1101 4 0.12778651040425615 1101 5 0.21583025568371994 1101 6 0.07114977643354337 1101 7 0.200458604324659 1101 8 0.20071440644516514 1101 9 0.20384602748600855 1101 10-11 0.17833311628969284 1101 12-13 0.23982474584204994 1101 14-15 0.3179490403339571 1101 16-17 0.1775505268881048 1101 18-19 0.20863213749668574 1101 20-21 0.08525887521530962 1101 22-23 0.08955519329680328 1101 24-25 0.0071919887082287914 1101 26-27 -0.026112838460697674 1101 28-29 -0.07908169052174685 1101 30-31 -0.13964585647686079 1101 32-33 -0.0715384248143991 1101 34-35 0.01297618068180384 1101 36-37 0.1379366520539591 1101 38-39 0.1328530015913998 1101 40-41 0.05707614951259643 1101 42-43 0.3646984146119223 1101 44-45 0.2935269167387631 1101 46-47 0.3947201092187598 1101 48-49 0.24542312347537631 1101 50-51 0.19746367160325207 1101 52-53 0.08549521033863527 1101 54-55 0.04520797226796702 1101 56-57 0.10856604610085441 1101 58-59 0.05446726966398785 1101 60-61 0.07577271921741868 1101 62-63 -0.026173490917479114 1101 64-65 -0.04145494201410571 1101 66-67 -0.21333284896392257 1101 68-69 -0.33093686652938104 1101 70-71 -0.36928396193126645 1101 72-73 -0.30044040315275566 1101 74-75 -0.18455703232447007 1101 76-77 0.24253865396047658 1101 78-79 0.12369181071714408 1101 80-81 -0.08248245922635178 1101 82-83 0.012054307520909191 1101 84-85 0.12294933674404618 1101 86-87 -0.004558069346586535 1101 88-89 -0.07055298680555211 1101 90-91 -0.12257786676686422 1101 92-93 -0.01951198947272914 1101 94-95 -0.21306228138950445 1101 96-97 0.07695310176687897 1101 98-99 -0.1629944358502975 1101 100-101 -0.3254942756857808 1102 1 0.5568039779081353 1102 2 0.6034470612070955 1102 3 0.36191302810056314 1102 4 0.18420858648409677 1102 5 0.29955999371532016 1102 6 0.3071431808281986 1102 7 0.2545214502899711 1102 8 0.1868464636042333 1102 9 0.3059883193522097 1102 10-11 0.3438154572603054 1102 12-13 0.4714703195125267 1102 14-15 0.5806994952216797 1102 16-17 0.6101796819381562 1102 18-19 0.4730349600403443 1102 20-21 0.49194743124276386 1102 22-23 0.38906196275138427 1102 24-25 0.5058401242551724 1102 26-27 0.34324959346612616 1102 28-29 0.36109150811220303 1102 30-31 0.36686282425275607 1102 32-33 0.5318213449793561 1102 34-35 0.5019690176666813 1102 36-37 0.4350633934793606 1102 38-39 0.4440268678917434 1102 40-41 0.37935956856177455 1102 42-43 0.18183514970194636 1102 44-45 0.20281921169798522 1102 46-47 0.43205769883643796 1102 48-49 0.304051911628882 1102 50-51 0.5604203458140447 1102 52-53 0.48068118635810464 1102 54-55 0.6171407598071355 1102 56-57 0.6269260718689438 1102 58-59 0.5521123057883699 1102 60-61 0.6717966912138209 1102 62-63 0.6070390189748665 1102 64-65 0.5605660286832261 1102 66-67 0.47220161478051637 1102 68-69 0.3949772818120074 1102 70-71 0.4165073827232426 1102 72-73 0.48867807456787915 1102 74-75 0.07634267479823365 1102 76-77 0.32786823158149403 1102 78-79 0.6844782491969141 1102 80-81 0.616618910876273 1102 82-83 0.6670571659009745 1102 84-85 0.8387786295936159 1102 86-87 0.5421207169937681 1102 88-89 0.6232529142561027 1102 90-91 0.6466791357310164 1102 92-93 0.8903303836653258 1102 94-95 0.8033323414429816 1102 96-97 0.9580647556383823 1102 98-99 0.6854636920814059 1102 100-101 0.7016632679845038 1103 1 0.4435469916947987 1103 2 0.5090852340976788 1103 3 0.40461001270729824 1103 4 0.19156582473329564 1103 5 0.06956822755640246 1103 6 0.08589585431623448 1103 7 0.15059129736143717 1103 8 0.18333252130136657 1103 9 0.21492002867753968 1103 10-11 0.21425772498064077 1103 12-13 0.2753557887912663 1103 14-15 0.31037019178455694 1103 16-17 0.2721940610362523 1103 18-19 0.3044274833109881 1103 20-21 0.13568042384235213 1103 22-23 0.26634384127210353 1103 24-25 0.20057900103875426 1103 26-27 0.09901579254433557 1103 28-29 0.1434472401709641 1103 30-31 0.14551960361593075 1103 32-33 0.029627829631273528 1103 34-35 0.090566402920075 1103 36-37 0.1920302773721616 1103 38-39 0.21439332402646016 1103 40-41 0.25338782469447807 1103 42-43 0.19309291913431537 1103 44-45 0.21608473299050246 1103 46-47 0.12308933887700135 1103 48-49 0.2943570658125765 1103 50-51 0.1558937414701944 1103 52-53 0.12403035142654062 1103 54-55 0.09260014789216342 1103 56-57 0.06003289089513686 1103 58-59 0.04408832723652267 1103 60-61 0.054680845086238605 1103 62-63 -0.038330700572480225 1103 64-65 -0.029272457494961657 1103 66-67 -0.05960209848099396 1103 68-69 -0.1386818070431879 1103 70-71 0.07645451543870507 1103 72-73 0.0188533449290631 1103 74-75 0.15567874605108045 1103 76-77 0.14325898758868405 1103 78-79 0.09093378143440844 1103 80-81 -0.010891406528255487 1103 82-83 0.08466177082722481 1103 84-85 0.17958308590906924 1103 86-87 0.08308432743267247 1103 88-89 -0.10009027625690692 1103 90-91 -0.09261812601475583 1103 92-93 0.007690985739852607 1103 94-95 -0.018411525851600885 1103 96-97 -0.20813833908091084 1103 98-99 -0.209958744131427 1103 100-101 -0.0016059183714176584 1104 1 0.39845730270267055 1104 2 0.4797606291633123 1104 3 0.2646740678932993 1104 4 0.08334306285200199 1104 5 0.10304586103404034 1104 6 0.19463481183567666 1104 7 0.12520620368366053 1104 8 0.09285515765327546 1104 9 0.21705285520060613 1104 10-11 0.16649203087348496 1104 12-13 0.11940839381947654 1104 14-15 0.38917449497910184 1104 16-17 0.09168396589200967 1104 18-19 0.23751210420048352 1104 20-21 0.144378320345119 1104 22-23 0.29628670780033417 1104 24-25 0.3754250222262385 1104 26-27 0.3773261903669294 1104 28-29 0.23093184228971353 1104 30-31 0.3020606235715846 1104 32-33 0.22132881157616424 1104 34-35 0.26639788850765456 1104 36-37 0.25283695037395404 1104 38-39 0.1028657368891146 1104 40-41 0.07084696898289877 1104 42-43 0.3294248798565249 1104 44-45 0.4458809530306169 1104 46-47 0.22469541121478898 1104 48-49 0.4087589241168743 1104 50-51 0.6259109870971926 1104 52-53 0.5367451885316186 1104 54-55 0.48535581137953443 1104 56-57 0.4023656259108037 1104 58-59 0.43010646447417145 1104 60-61 0.31892843128504467 1104 62-63 0.06698259498471515 1104 64-65 0.20759564278777276 1104 66-67 0.5253226425413118 1104 68-69 0.3018090480818181 1104 70-71 0.13611969217705067 1104 72-73 0.34074121315839534 1104 74-75 0.3657070194939891 1104 76-77 0.5097625833605726 1104 78-79 0.44371713177873673 1104 80-81 0.3810676732357017 1104 82-83 0.5835765868152123 1104 84-85 0.5228860340900674 1104 86-87 0.7275627938623579 1104 88-89 0.6928530947352485 1104 90-91 0.6364565933038655 1104 92-93 0.6960472545839238 1104 94-95 0.5562209780491969 1104 96-97 0.5567732624160548 1104 98-99 0.8437654900806137 1104 100-101 0.8430121555149483 1105 1 0.4043832286285962 1105 2 0.39339870483528827 1105 3 0.2024107568164304 1105 4 0.06616335618076619 1105 5 0.008260721503134505 1105 6 0.13374797738952537 1105 7 0.11563706597893031 1105 8 0.13310397349530945 1105 9 0.007417518785608479 1105 10-11 -0.022518983222276745 1105 12-13 -0.012214045260584783 1105 14-15 0.08136647123887997 1105 16-17 0.086128981014987 1105 18-19 0.13907462203419385 1105 20-21 0.08655032285610531 1105 22-23 0.07446053648264694 1105 24-25 -0.08123993240482008 1105 26-27 -0.10991115483651015 1105 28-29 -0.03775160232593322 1105 30-31 0.08766925223105915 1105 32-33 0.04348597644519003 1105 34-35 -0.09452661072192825 1105 36-37 -0.08014712769487176 1105 38-39 -0.040179403783149326 1105 40-41 0.1912464439586401 1105 42-43 0.02113520343124975 1105 44-45 -0.02642515893651165 1105 46-47 0.4496032466310922 1105 48-49 0.30175327439935984 1105 50-51 0.23133813998197894 1105 52-53 0.32606779310845724 1105 54-55 0.36608742126368554 1105 56-57 0.3156202056060593 1105 58-59 0.22916598624555462 1105 60-61 0.3255460195975459 1105 62-63 0.20879621137461157 1105 64-65 0.2952016463259781 1105 66-67 0.4909677946271387 1105 68-69 0.37067625118630687 1105 70-71 0.3332560272794858 1105 72-73 0.17451625167919715 1105 74-75 0.25182355205119933 1105 76-77 0.026381883708683773 1105 78-79 0.1823281002202144 1105 80-81 0.03571699127484962 1105 82-83 0.1441267209246675 1105 84-85 0.30517217230841 1105 86-87 0.23951850920790463 1105 88-89 0.3115142828879591 1105 90-91 0.06259595300254617 1105 92-93 0.15661793312070316 1105 94-95 0.23003824678171725 1105 96-97 0.1587192762264742 1105 98-99 0.16904084247460816 1105 100-101 0.4893135879180761 1106 1 0.30352433292307524 1106 2 0.30382953912179644 1106 3 0.0621457168986943 1106 4 -0.12348183231645038 1106 5 -0.24259459258187377 1106 6 -0.06811538956483076 1106 7 -0.06757229707029921 1106 8 -0.05079814428223273 1106 9 -0.13017401472564671 1106 10-11 -0.16767582353976707 1106 12-13 -0.098422264501977 1106 14-15 -0.05256395289431737 1106 16-17 0.01591812754949018 1106 18-19 -0.03627897633905519 1106 20-21 -0.09034106161352184 1106 22-23 -0.12651389536836177 1106 24-25 -0.09897542395334824 1106 26-27 0.02510045654610593 1106 28-29 0.1273500117140216 1106 30-31 -0.02432594146528544 1106 32-33 0.08235626401465623 1106 34-35 -0.1188472004184078 1106 36-37 0.022657858845171575 1106 38-39 0.14091287493624804 1106 40-41 -0.016364615161201357 1106 42-43 -0.13411233276044499 1106 44-45 -0.017563305505660765 1106 46-47 0.1517099327078384 1106 48-49 -0.011486474656123846 1106 50-51 0.0026724669261639633 1106 52-53 0.24359693710115948 1106 54-55 0.10719795469407245 1106 56-57 0.08327285773827953 1106 58-59 0.0553300706793749 1106 60-61 0.3376535144273092 1106 62-63 -0.16927156212832983 1106 64-65 0.13301755103177015 1106 66-67 0.05019508888614865 1106 68-69 0.014380571082785565 1106 70-71 0.025347347048878532 1106 72-73 0.04494893454403126 1106 74-75 0.04610197105458269 1106 76-77 0.13118333484267097 1106 78-79 0.251837187138058 1106 80-81 0.12632598229768988 1106 82-83 0.06139567751773711 1106 84-85 -0.022056231097597134 1106 86-87 -0.06593248613598846 1106 88-89 -0.1424260773537398 1106 90-91 -0.15822643328100128 1106 92-93 0.17663017995212726 1106 94-95 0.11715134511453229 1106 96-97 -0.015642097729369198 1106 98-99 -0.027259690147019455 1106 100-101 0.29892919916205685 1107 1 0.4521240924159713 1107 2 0.5714667266833651 1107 3 0.33837956136623504 1107 4 0.135954448021522 1107 5 0.039169556460059596 1107 6 0.1982003677268196 1107 7 0.23034172277267118 1107 8 0.37774950533832197 1107 9 0.3478995279077495 1107 10-11 0.3717794006957291 1107 12-13 0.36245569965748103 1107 14-15 0.4851270996526438 1107 16-17 0.5067252983752084 1107 18-19 0.31343607256631145 1107 20-21 0.26819399648638154 1107 22-23 0.4283546183566216 1107 24-25 0.43450250804790613 1107 26-27 0.33436949159627716 1107 28-29 0.3532681623004592 1107 30-31 0.3756184610829152 1107 32-33 0.4445091196401876 1107 34-35 0.3330771394892338 1107 36-37 0.2918256748277912 1107 38-39 0.3566751488380646 1107 40-41 0.47994766587429183 1107 42-43 0.6605408569027276 1107 44-45 0.6141055578831285 1107 46-47 0.769726564385472 1107 48-49 0.7516437524668191 1107 50-51 0.6888610860938087 1107 52-53 0.4398171596268483 1107 54-55 0.6154832205767136 1107 56-57 0.6968167819898667 1107 58-59 0.3835279998724488 1107 60-61 0.5864388063535628 1107 62-63 0.4906701907653357 1107 64-65 0.5556436757195229 1107 66-67 0.4264666965923425 1107 68-69 0.4914879522956035 1107 70-71 0.3809355137370609 1107 72-73 0.6781090778518433 1107 74-75 0.992837404182513 1107 76-77 0.7760237554356983 1107 78-79 0.8233501172256759 1107 80-81 0.7130673488400276 1107 82-83 0.616634463477741 1107 84-85 0.7911623744594287 1107 86-87 0.9353572388661071 1107 88-89 1.1626700349010228 1107 90-91 1.1900372503728924 1107 92-93 1.4524037318706355 1107 94-95 1.2016456007994023 1107 96-97 1.1320285841830042 1107 98-99 1.2740397162850705 1107 100-101 1.2285010421666271 1108 1 0.28223076226088395 1108 2 0.28600694886254985 1108 3 0.031322616156199246 1108 4 0.05143051434752266 1108 5 -0.16921033319525236 1108 6 -0.056395672378663164 1108 7 0.03172127970546512 1108 8 0.02581102424142756 1108 9 0.0985041393424737 1108 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-1.3656828131833656 2316 92-93 -1.5349778739567306 2316 94-95 -1.892030241630593 2316 96-97 -1.7996071641507072 2316 98-99 -1.9878538682468232 2316 100-101 -2.5910187778948632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 17.0 5 41.0 6 104.0 7 284.0 8 645.0 9 1212.0 10 2003.0 11 3244.0 12 4850.0 13 6645.0 14 8186.0 15 10935.0 16 14194.0 17 18169.0 18 15435.0 19 9375.0 20 7975.0 21 8101.0 22 8568.0 23 9404.0 24 11168.0 25 13254.0 26 16111.0 27 19977.0 28 24859.0 29 30643.0 30 37834.0 31 46541.0 32 58103.0 33 73670.0 34 97857.0 35 131716.0 36 183967.0 37 237075.0 38 144887.0 39 9133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34003777487611 19.101628791962657 14.942479165917957 21.61585426724327 2 15.861708354100038 21.15968655241622 39.24698017217343 23.731624921310313 3 19.97371515856599 25.492663069876762 30.205689941546243 24.327931830011 4 12.951245038605084 17.070910921980214 36.768942161489704 33.208901877925 5 13.048831533971994 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20.988911616739983 26-27 21.617103916909752 25.435450222893174 31.624729157839326 21.322716702357745 28-29 21.11973716424865 26.05055323450667 31.613778342902727 21.215931258341953 30-31 21.386008418957662 25.53234506669615 31.497168671368435 21.584477842977755 32-33 21.179505770330422 25.675758925609653 31.428950535963075 21.71578476809685 34-35 21.997789412954482 25.48710510634551 31.170896385544072 21.34420909515594 36-37 22.1459630162157 25.29393250954962 31.147309223752806 21.412795250481874 38-39 21.729062564909345 25.599811718471727 31.263223736740724 21.4079019798782 40-41 21.781884582790013 25.631152079119403 31.16258351189484 21.424379826195743 42-43 21.55345672259532 25.975802645635333 30.57239163617479 21.898348995594556 44-45 21.810838207982485 25.83359363492514 30.32595814350404 22.029610013588336 46-47 22.370109740831566 26.347829864196214 29.516808630786667 21.765251764185553 48-49 22.485044223602877 26.526737152723474 28.961602317730673 22.026616305942976 50-51 22.23979029928869 26.850686570016798 29.010717623918453 21.89880550677606 52-53 22.643470500312954 26.955493293685816 28.601891944722517 21.79914426127871 54-55 21.962529901082068 26.80696923777467 28.862102153193852 22.368398707949414 56-57 22.498920931635837 27.065561942868023 28.324547433001996 22.110969692494148 58-59 22.713460444152048 26.539421584303156 28.39494521055897 22.352172760985827 60-61 22.218005428474733 26.760176810468987 29.136447591287112 21.885370169769168 62-63 22.333821828424885 26.758829144004164 29.064115276943546 21.8432337506274 64-65 21.97122742577288 27.016992999397694 28.92787108622886 22.08390848860057 66-67 22.01063144597064 27.04305455240475 28.47226966483816 22.474044336786452 68-69 22.25770058360281 26.792538066074783 28.68795394675271 22.261807403569694 70-71 22.336284220244785 27.005669841325002 28.384114966912104 22.273930971518112 72-73 22.313001998120345 26.865557301826744 28.48810210158033 22.333338598472583 74-75 22.12748481665469 27.62997654380464 28.02742874292168 22.21510989661899 76-77 22.30641711736757 27.13137826021027 28.207097591488072 22.35510703093409 78-79 22.209268434858643 27.144779900145256 28.333013437974657 22.31293822702144 80-81 22.377375465890882 27.3993010205069 27.93469292967511 22.288630583927105 82-83 22.286679957536617 27.310249661935877 28.096683180203975 22.30638720032353 84-85 22.173010561002407 27.313788383450245 27.93796250295204 22.57523855259531 86-87 22.2901544172821 27.288693179574263 27.711701749535962 22.709450653607675 88-89 22.23061390231031 27.209939295499147 27.598585563009753 22.960861239180787 90-91 22.10548684366961 27.105670511310965 27.946552321381247 22.842290323638185 92-93 22.23844808767338 27.0370744790918 28.0590670645119 22.66541036872292 94-95 21.854689881258683 26.929679721879847 28.196037901492925 23.01959249536855 96-97 21.872634119389865 26.81996816070197 28.251630766824732 23.055766953083438 98-99 21.72026831430766 26.947539695233807 27.73163040237273 23.600561588085803 100-101 21.417555566350444 26.893999082961205 28.114861281097664 23.573584069590687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 432.0 1 357.5 2 564.5 3 782.5 4 557.0 5 434.5 6 673.5 7 1329.5 8 2067.0 9 2502.5 10 2328.0 11 1919.5 12 1903.0 13 2011.0 14 2176.5 15 2397.0 16 2558.5 17 2731.5 18 2887.0 19 3080.0 20 3372.5 21 3762.0 22 4253.5 23 4839.5 24 5540.5 25 6573.5 26 8283.5 27 10096.5 28 11606.0 29 13509.0 30 15946.5 31 18787.5 32 22104.5 33 24067.5 34 25556.5 35 27496.0 36 30061.0 37 32639.0 38 33833.5 39 34090.0 40 34180.0 41 36019.0 42 37639.0 43 38715.0 44 40250.5 45 41962.5 46 42591.0 47 45286.5 48 54484.0 49 53978.0 50 45528.0 51 41865.5 52 39638.5 53 39577.0 54 39283.0 55 37190.0 56 34190.5 57 31332.0 58 29473.5 59 26576.5 60 22362.5 61 18966.0 62 16198.5 63 12459.0 64 8762.5 65 6571.0 66 5006.0 67 3890.5 68 2872.0 69 2002.0 70 1539.0 71 1223.0 72 1031.0 73 966.5 74 777.5 75 600.0 76 463.5 77 264.0 78 160.0 79 125.0 80 97.5 81 69.5 82 45.5 83 31.0 84 20.5 85 12.0 86 6.0 87 3.5 88 4.0 89 2.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.108443440557894 2 0.011135769513264202 3 0.0048176024135398315 4 0.013031219643181512 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004185785703567395 10-11 2.3693126623966387E-4 12-13 1.9744272186638653E-4 14-15 3.948854437327731E-5 16-17 0.0 18-19 0.0 20-21 3.948854437327731E-5 22-23 0.0013031219643181513 24-25 0.0 26-27 3.948854437327731E-5 28-29 0.0 30-31 0.0 32-33 0.004580671147300168 34-35 8.292594318388235E-4 36-37 0.003198572094235462 38-39 7.897708874655462E-5 40-41 0.004185785703567395 42-43 0.011254235146384035 44-45 0.0024088012067699158 46-47 0.0019744272186638656 48-49 0.009793159004572774 50-51 3.553968993594958E-4 52-53 0.002566755384263025 54-55 0.011096280968890924 56-57 0.004067320070447563 58-59 0.0019349386742905882 60-61 0.006989472354070084 62-63 0.004462205514180336 64-65 0.017967287689841176 66-67 0.003198572094235462 68-69 2.7641981061294116E-4 70-71 0.0013426105086914288 72-73 0.0 74-75 0.0 76-77 0.0011056792424517646 78-79 0.011925540400729748 80-81 0.015913883382430757 82-83 0.01247838002195563 84-85 0.009240319383346891 86-87 0.011056792424517648 88-89 0.030485156256170085 90-91 0.02499624858828454 92-93 0.0028431751948759664 94-95 0.009358785016466723 96-97 0.011412189323877144 98-99 0.010859349702651261 100-101 0.012517868566328907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1266190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.04239255265182 #Duplication Level Percentage of deduplicated Percentage of total 1 80.6474490855687 46.809708981945796 2 11.55325566499497 13.41157201137573 3 3.2640079594511135 5.683524938323247 4 1.427306511212133 3.313771392669223 5 0.7549467002512126 2.1909456366155022 6 0.4729696492089814 1.6471374026926635 7 0.3293355639697524 1.3380796859836943 8 0.23320945397547502 1.0828827739707287 9 0.15842138244276913 0.8275640461629289 >10 1.0284669080975508 11.265537584720017 >50 0.07924346652504846 3.1340930272889125 >100 0.04592089625346649 4.909410546542998 >500 0.0035533927317607434 1.4058934426021803 >1k 0.0016400274146588045 1.7811844134847012 >5k 2.733379024431341E-4 1.1986941156215962 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8018 0.633238297569875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7093 0.560184490479312 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4577 0.3614781351929805 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3897 0.3077737148453234 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1989 0.15708542951689716 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1746 0.13789399695148435 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1489 0.11759688514361984 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1415 0.11175258057637479 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1318 0.104091802967959 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.10124862777308302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.897708874655462E-5 0.0 10-11 0.0 0.0 0.0 7.897708874655462E-5 0.0 12-13 0.0 0.0 0.0 7.897708874655462E-5 0.0 14-15 0.0 0.0 0.0 1.1846563311983192E-4 1.1846563311983192E-4 16-17 0.0 0.0 0.0 2.3693126623966387E-4 1.5795417749310924E-4 18-19 0.0 0.0 0.0 2.3693126623966387E-4 1.5795417749310924E-4 20-21 0.0 0.0 0.0 3.159083549862185E-4 1.5795417749310924E-4 22-23 0.0 0.0 0.0 5.13351076852605E-4 1.5795417749310924E-4 24-25 0.0 0.0 0.0 6.31816709972437E-4 1.5795417749310924E-4 26-27 0.0 0.0 0.0 0.0010661906980784874 2.3693126623966387E-4 28-29 1.1846563311983192E-4 0.0 0.0 0.0016980074080509243 2.3693126623966387E-4 30-31 1.5795417749310924E-4 0.0 0.0 0.0027247095617561343 2.3693126623966387E-4 32-33 1.5795417749310924E-4 0.0 0.0 0.0046201596916734455 2.3693126623966387E-4 34-35 1.5795417749310924E-4 0.0 0.0 0.006278678555351093 2.3693126623966387E-4 36-37 1.5795417749310924E-4 0.0 0.0 0.009793159004572774 2.3693126623966387E-4 38-39 1.5795417749310924E-4 0.0 0.0 0.015045135406218657 2.3693126623966387E-4 40-41 1.5795417749310924E-4 0.0 0.0 0.02084995142909042 2.3693126623966387E-4 42-43 1.5795417749310924E-4 0.0 0.0 0.029734873913077817 2.3693126623966387E-4 44-45 2.3693126623966387E-4 0.0 0.0 0.03719820879962722 2.3693126623966387E-4 46-47 2.3693126623966387E-4 0.0 0.0 0.05153255040712689 2.3693126623966387E-4 48-49 3.159083549862185E-4 0.0 0.0 0.07009216626256723 2.3693126623966387E-4 50-51 3.159083549862185E-4 0.0 0.0 0.07893760020218135 2.3693126623966387E-4 52-53 3.159083549862185E-4 0.0 0.0 0.0901523468041921 2.3693126623966387E-4 54-55 3.948854437327731E-4 0.0 0.0 0.10847503139339278 2.3693126623966387E-4 56-57 3.948854437327731E-4 0.0 0.0 0.13880223347206977 2.3693126623966387E-4 58-59 3.948854437327731E-4 0.0 0.0 0.17817231221222724 2.3693126623966387E-4 60-61 3.948854437327731E-4 0.0 0.0 0.2222415277328047 2.7641981061294116E-4 62-63 3.948854437327731E-4 0.0 0.0 0.273260727063079 3.159083549862185E-4 64-65 3.948854437327731E-4 0.0 0.0 0.3269651474107361 3.159083549862185E-4 66-67 5.528396212258823E-4 0.0 0.0 0.4042837172936131 3.159083549862185E-4 68-69 5.528396212258823E-4 0.0 0.0 0.500951673919396 3.159083549862185E-4 70-71 6.713052543457143E-4 0.0 0.0 0.5511811023622046 3.159083549862185E-4 72-73 7.107937987189916E-4 0.0 0.0 0.621431222802265 3.159083549862185E-4 74-75 7.107937987189916E-4 0.0 0.0 0.6977625790758102 3.159083549862185E-4 76-77 7.107937987189916E-4 0.0 0.0 0.783610674543315 3.159083549862185E-4 78-79 7.107937987189916E-4 0.0 0.0 0.8734076244481477 3.159083549862185E-4 80-81 8.687479762121009E-4 0.0 0.0 1.005180897021774 3.159083549862185E-4 82-83 8.687479762121009E-4 0.0 0.0 1.151564931013513 3.159083549862185E-4 84-85 9.477250649586554E-4 0.0 0.0 1.380401045656655 3.159083549862185E-4 86-87 0.00102670215370521 0.0 0.0 1.7113940245934653 3.159083549862185E-4 88-89 0.00102670215370521 0.0 0.0 2.058774749445186 3.553968993594958E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1250 0.0 31.924553 2 TATCACG 90 2.4108034E-5 31.671185 2 GTACACG 220 1.8189894E-12 28.386168 1 GTACCTG 1500 0.0 27.221605 1 ATCACGC 110 9.475818E-5 25.909716 3 GTACATG 7695 0.0 25.283226 1 TACATGG 7865 0.0 24.523521 2 ACATGGG 7665 0.0 24.478737 3 GTATCAA 13760 0.0 24.019064 1 GGTATCA 10290 0.0 23.995722 1 CCTATAC 285 3.6379788E-12 23.333899 3 TCACGCA 165 2.4305318E-6 23.029947 4 TAAGGTG 565 0.0 22.694284 5 CATGGGG 2935 0.0 22.495396 4 GAGTACT 8645 0.0 22.055744 12-13 GTACTTT 9085 0.0 20.909143 14-15 AGTACTT 8805 0.0 20.84593 12-13 GGACCGT 115 0.0037280791 20.647778 6 CATGGGA 4570 0.0 20.579552 4 ATCAACG 16615 0.0 19.926752 3 >>END_MODULE