FastQCFastQC Report
Thu 26 May 2016
SRR936477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265793
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64300.5079819528153497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.4172088169234622No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35080.2771385210694008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.23882261949623676No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20170.15934674942901406No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14310.11305166010556228No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13920.10997058760792641No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13890.10973358203118519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG14200.031.4567741
TACCTGG13900.031.110092
GTACATG79650.026.4893821
TACATGG81400.025.3944912
GTACGTG1351.3385366E-524.63991
ACATGGG81100.024.3165453
GTACCGA1000.001649729923.7599016
TAAGGTA4150.022.903834
GTACACG2303.610694E-922.7268641
GTATATG5050.022.5836721
GTATCAA124800.022.1987571
GAGTACT58600.021.57298312-13
CATGGGG28700.021.361614
CATGGGA48300.021.1552184
GGTATCA95500.020.7992441
AGTACTT60600.020.42966712-13
GTACTTT63150.020.09309814-15
GTACCCG1707.929224E-519.5669781
GTAAGGT4650.019.4166973
ATGGGAG20000.019.2470425
Produced by FastQC (version 0.11.5)