FastQCFastQC Report
Thu 26 May 2016
SRR936477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265793
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80440.6354909531021264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71530.565100296809984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45500.3594584580575181No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37280.2945189300304236No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.17056501339476518No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18260.14425739437648966No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14760.11660674375668059No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14410.11384167869469969No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13500.10665250953354932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.10333443145917223No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13060.10317642774134476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.10214940357546613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12200.033.0932852
GTACCTG14000.030.8899541
GTACATG73650.025.3585071
GTACACG3000.025.3456021
GTATCAA139350.023.9746481
TAACGCA1201.7117505E-423.7483624
TACATGG77050.023.672242
GGTATCA104300.022.8274461
ACATGGG74900.022.575213
GAGTACT82750.022.30126812-13
TAAGGTG6250.022.0384795
AGTACTT84450.021.6554712-13
GTACTTT89350.020.4940514-15
GTAAGGT5850.020.2977454
TCAACGC164200.020.2482974
ATCAACG164300.020.2359733
CAACGCA165350.020.1649235
TATCAAC167100.020.011382
AACGCAG168200.019.8805076
CATGGGG28600.019.4304774
Produced by FastQC (version 0.11.5)