##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936477_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1265793 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87297844118272 31.0 31.0 34.0 30.0 34.0 2 32.008631743104914 31.0 31.0 34.0 30.0 34.0 3 32.10238956922657 33.0 31.0 34.0 30.0 34.0 4 35.862773771066834 37.0 35.0 37.0 35.0 37.0 5 35.652556144646084 37.0 35.0 37.0 33.0 37.0 6 35.764865187277856 37.0 35.0 37.0 33.0 37.0 7 35.70981037183805 37.0 35.0 37.0 33.0 37.0 8 35.689565355472816 37.0 35.0 37.0 33.0 37.0 9 37.3181863069238 39.0 37.0 39.0 34.0 39.0 10-11 37.1871664640269 39.0 37.0 39.0 33.0 39.0 12-13 37.09152325854227 39.0 37.0 39.0 33.0 39.0 14-15 38.154601897782655 40.0 37.0 41.0 33.0 41.0 16-17 38.08688347936827 40.0 37.0 41.0 33.0 41.0 18-19 38.276860829535316 40.0 38.0 41.0 33.5 41.0 20-21 38.320693430916435 40.0 38.0 41.0 34.0 41.0 22-23 38.225203489038094 40.0 38.0 41.0 33.5 41.0 24-25 38.09454902973867 40.0 37.0 41.0 33.0 41.0 26-27 37.865235073981296 40.0 37.0 41.0 33.0 41.0 28-29 37.762530682346956 40.0 37.0 41.0 33.0 41.0 30-31 37.67280550611356 40.0 37.0 41.0 33.0 41.0 32-33 37.45936420883983 39.0 36.0 41.0 32.5 41.0 34-35 37.256171427713696 39.0 36.0 41.0 32.0 41.0 36-37 36.95717941243157 39.0 35.5 41.0 31.0 41.0 38-39 36.76562123506766 39.0 35.0 41.0 31.0 41.0 40-41 36.520980523671724 39.0 35.0 41.0 30.5 41.0 42-43 36.21971680993654 39.0 35.0 40.0 30.0 41.0 44-45 35.88851376172882 39.0 35.0 40.0 29.5 41.0 46-47 35.417567880372225 38.0 35.0 40.0 27.5 41.0 48-49 34.95890560304884 38.0 34.5 40.0 25.5 41.0 50-51 34.59418799124343 38.0 34.0 40.0 24.0 41.0 52-53 34.29813208004784 38.0 34.0 40.0 23.5 41.0 54-55 34.0213964684589 38.0 33.0 40.0 23.0 41.0 56-57 33.706191691690506 37.5 33.0 40.0 22.0 41.0 58-59 33.35851952096433 37.0 33.0 40.0 21.0 41.0 60-61 32.99099931821395 36.5 32.0 39.5 19.5 41.0 62-63 32.589043390190966 36.0 32.0 39.0 18.0 40.0 64-65 32.16884316787974 35.5 31.0 39.0 17.5 40.0 66-67 31.705740196066813 35.0 31.0 38.0 13.5 40.0 68-69 31.420189557060276 35.0 31.0 38.0 12.0 40.0 70-71 30.9854980237685 35.0 30.0 37.0 8.0 39.5 72-73 30.533645311674185 34.0 30.0 37.0 7.0 39.0 74-75 30.021543806925777 34.0 29.0 36.0 6.0 39.0 76-77 28.63295538844029 32.5 27.0 35.0 2.0 37.0 78-79 29.395522411642347 34.0 29.0 35.0 2.0 37.0 80-81 29.19863713893188 34.0 29.0 35.0 2.0 37.0 82-83 28.865265884706268 34.0 29.0 35.0 2.0 36.5 84-85 28.451321029583823 34.0 28.5 35.0 2.0 36.0 86-87 28.070429762212306 33.0 27.0 35.0 2.0 36.0 88-89 27.65400069363632 33.0 26.5 35.0 2.0 35.0 90-91 27.169048177703623 33.0 26.0 35.0 2.0 35.0 92-93 26.680298437422234 32.5 25.0 34.5 2.0 35.0 94-95 26.092559762931224 32.0 24.5 34.0 2.0 35.0 96-97 25.31840830214735 32.0 21.5 34.0 2.0 35.0 98-99 24.37026670237551 31.5 10.0 34.0 2.0 35.0 100-101 21.166829015486734 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3629825769444359 1101 2 0.4546357171762203 1101 3 0.4002387643362795 1101 4 0.31806050463600855 1101 5 0.2934514867488005 1101 6 0.32983884294790755 1101 7 0.35289372920352946 1101 8 0.3447629066543314 1101 9 0.42414083585431683 1101 10-11 0.5371443645783955 1101 12-13 0.41780437196734255 1101 14-15 0.6463049457051255 1101 16-17 0.6182328846141516 1101 18-19 0.5555839121817883 1101 20-21 0.5018599041760794 1101 22-23 0.3800220266643848 1101 24-25 0.33756297863185125 1101 26-27 0.4066610197545302 1101 28-29 0.37941962518157624 1101 30-31 0.5001149314234539 1101 32-33 0.5938468915312356 1101 34-35 0.4464678614259441 1101 36-37 0.4763940630076533 1101 38-39 0.5301947122303545 1101 40-41 0.6979013295956165 1101 42-43 0.7177974226448072 1101 44-45 0.7554726413885291 1101 46-47 0.6460945165139123 1101 48-49 0.642662895902653 1101 50-51 0.7612217277695521 1101 52-53 0.6649348829741797 1101 54-55 0.5846770347745576 1101 56-57 0.5835299013968083 1101 58-59 0.5773429396451775 1101 60-61 0.3954024295438856 1101 62-63 0.4253587782939974 1101 64-65 0.516554499179108 1101 66-67 0.4998316602025703 1101 68-69 0.44036534450244247 1101 70-71 0.45482799985775557 1101 72-73 0.6532408355660664 1101 74-75 0.5023229485342142 1101 76-77 0.43867480435065787 1101 78-79 0.3918347311658614 1101 80-81 0.6110774205517338 1101 82-83 0.7221919276340962 1101 84-85 0.6846162050920093 1101 86-87 0.6481282631684735 1101 88-89 0.521728481274284 1101 90-91 0.41861813605876463 1101 92-93 0.4656784170146224 1101 94-95 0.547452989636632 1101 96-97 0.7805722002495692 1101 98-99 0.9266679261519428 1101 100-101 1.3812636722343754 1102 1 0.3829215068983025 1102 2 0.3847142867036517 1102 3 0.3542601870545852 1102 4 0.2523745773625947 1102 5 0.21864277598903215 1102 6 0.2612995571363186 1102 7 0.21786060593382928 1102 8 0.2202556689946107 1102 9 0.12494371624798362 1102 10-11 0.3957317896816619 1102 12-13 0.3147030866461549 1102 14-15 0.4621239528662855 1102 16-17 0.33860165212672655 1102 18-19 0.3638503737447536 1102 20-21 0.07001095299229831 1102 22-23 0.1459203578216446 1102 24-25 0.3010854670371401 1102 26-27 0.0266671183761531 1102 28-29 0.1419778607308828 1102 30-31 0.3712972676356543 1102 32-33 0.3588466714778775 1102 34-35 0.35631985982479364 1102 36-37 0.5392523046346298 1102 38-39 0.4371189530986612 1102 40-41 0.42927800435884933 1102 42-43 0.4525954031837074 1102 44-45 0.47885194840907275 1102 46-47 0.4598864131014082 1102 48-49 0.5047711747291146 1102 50-51 0.44146795699808905 1102 52-53 0.410114142630853 1102 54-55 0.47921384341406537 1102 56-57 0.6254526857214628 1102 58-59 0.35078281373274933 1102 60-61 0.4695452169283314 1102 62-63 0.4304257688233193 1102 64-65 0.45936001183007136 1102 66-67 0.3262907711450893 1102 68-69 0.38117675458340017 1102 70-71 0.4939482474911365 1102 72-73 0.4220533019031407 1102 74-75 0.4668660653699739 1102 76-77 0.14121348658732558 1102 78-79 0.08874004744540898 1102 80-81 0.6128331319842353 1102 82-83 0.4941249601579827 1102 84-85 0.6553485845813576 1102 86-87 0.5248653748528866 1102 88-89 0.18248269843970277 1102 90-91 0.31094917175560965 1102 92-93 0.3001338478267037 1102 94-95 0.4162624171416276 1102 96-97 0.6110326147462608 1102 98-99 0.3475571722491395 1102 100-101 0.9205961141769627 1103 1 0.1648657373408362 1103 2 0.17549449159237795 1103 3 0.1354725309484195 1103 4 0.1571157404004495 1103 5 -0.021251065891867427 1103 6 0.17023845004185745 1103 7 0.14914031104577674 1103 8 0.11033243312762409 1103 9 0.2886225869499981 1103 10-11 0.1540630488425876 1103 12-13 0.2521277744338235 1103 14-15 0.11215822609603521 1103 16-17 0.272465677285453 1103 18-19 0.173639381026085 1103 20-21 0.18024975937522214 1103 22-23 0.21355566300153583 1103 24-25 0.15533979218045602 1103 26-27 0.23807664692664332 1103 28-29 0.157150925725567 1103 30-31 0.19228927515797523 1103 32-33 0.16919619915919526 1103 34-35 0.12746061513961848 1103 36-37 0.07692223861668168 1103 38-39 0.029460614728051837 1103 40-41 0.25823779702062666 1103 42-43 0.35578538157280803 1103 44-45 0.4730813044674349 1103 46-47 0.45163515773325713 1103 48-49 0.25994524717896894 1103 50-51 0.2743647264251834 1103 52-53 0.3191177299614125 1103 54-55 0.10170903330948278 1103 56-57 0.16787099295973462 1103 58-59 0.4382546148650448 1103 60-61 0.2597234179689778 1103 62-63 -0.0648940092994934 1103 64-65 -0.04257390701249619 1103 66-67 0.13730403399607027 1103 68-69 0.2686682109684959 1103 70-71 0.3535475173824487 1103 72-73 0.15831240356919807 1103 74-75 0.36879524324704605 1103 76-77 0.11401120934356257 1103 78-79 -0.10680008680293085 1103 80-81 0.023281232239856564 1103 82-83 0.20282028596108503 1103 84-85 0.18412871440479606 1103 86-87 0.11216580983647262 1103 88-89 0.1080949743796964 1103 90-91 -0.007184428652109887 1103 92-93 0.11976085236618061 1103 94-95 0.30534752932472387 1103 96-97 0.37248994803218594 1103 98-99 0.4706914047855939 1103 100-101 0.6285249838912854 1104 1 0.2520696747393636 1104 2 0.39475745522028305 1104 3 0.2973236256247489 1104 4 0.1569936378033816 1104 5 0.14815639385287938 1104 6 0.18058531794132904 1104 7 0.23775204795280303 1104 8 0.1171528042809058 1104 9 0.19656350280634882 1104 10-11 0.31165889214200604 1104 12-13 0.2211607701269358 1104 14-15 0.3373972190053962 1104 16-17 0.344453362780051 1104 18-19 0.27859862868103846 1104 20-21 0.18695743900116923 1104 22-23 0.18914889822809045 1104 24-25 0.19325506166522644 1104 26-27 0.3072296699552908 1104 28-29 0.3048665936605559 1104 30-31 0.15840459792389083 1104 32-33 0.32314103002055106 1104 34-35 0.173221097140825 1104 36-37 0.32420461124871025 1104 38-39 0.39066881268676923 1104 40-41 0.2676585191344145 1104 42-43 0.31916497875972283 1104 44-45 0.42082742565884246 1104 46-47 0.514101977679708 1104 48-49 0.563304806026629 1104 50-51 0.5111390364248507 1104 52-53 0.501836061953064 1104 54-55 0.44433881437421263 1104 56-57 0.5285140441154113 1104 58-59 0.4491646990917104 1104 60-61 0.5718096928648748 1104 62-63 0.5841852985090839 1104 64-65 0.8263390613450738 1104 66-67 0.5819206125365106 1104 68-69 0.8592709923980948 1104 70-71 0.7349376337322298 1104 72-73 0.3196393740541481 1104 74-75 0.5664532864781258 1104 76-77 0.49115283098187845 1104 78-79 0.4505847554868474 1104 80-81 0.6174131648199186 1104 82-83 0.4463343697475963 1104 84-85 0.553469524471069 1104 86-87 0.5871634932654608 1104 88-89 0.7510597412650881 1104 90-91 0.7033180356003008 1104 92-93 0.7884593448575927 1104 94-95 0.9086984492929844 1104 96-97 1.0286697873007675 1104 98-99 1.3606569361394172 1104 100-101 1.69230114907824 1105 1 0.06928137099761145 1105 2 0.13865240669212042 1105 3 0.0660790276907619 1105 4 0.05894304590828625 1105 5 -0.07397132526633499 1105 6 -0.017088414405577623 1105 7 0.024906363323040637 1105 8 0.04006841591853316 1105 9 0.09055164586638398 1105 10-11 0.02224499012739045 1105 12-13 -0.12112055804833943 1105 14-15 0.10179717559848456 1105 16-17 0.22546179285875212 1105 18-19 0.11981453617102034 1105 20-21 0.2093500735340328 1105 22-23 0.15403475519089938 1105 24-25 0.11747050081749677 1105 26-27 0.08825442522028482 1105 28-29 0.053926348601898155 1105 30-31 0.17974323464233066 1105 32-33 0.2611514895626428 1105 34-35 0.1685208237534539 1105 36-37 0.05367081097380577 1105 38-39 0.1940883442729131 1105 40-41 0.2022502086150979 1105 42-43 0.3312320519536698 1105 44-45 0.07814653497953117 1105 46-47 0.10398283713650613 1105 48-49 0.17650164874512342 1105 50-51 0.14735867538598768 1105 52-53 0.09704188304806394 1105 54-55 0.06296342456874271 1105 56-57 -0.17619094120620105 1105 58-59 -0.13179370833163517 1105 60-61 -0.18644690098734173 1105 62-63 0.11105427056493511 1105 64-65 -0.25285332264615334 1105 66-67 -0.11957882964662403 1105 68-69 0.058672195987917064 1105 70-71 0.046450507307703504 1105 72-73 0.09790550600353853 1105 74-75 -0.15097606306553502 1105 76-77 -0.1471391071366206 1105 78-79 -0.07955686907385129 1105 80-81 0.10859256671839645 1105 82-83 0.08877328594561007 1105 84-85 0.034695322263406325 1105 86-87 0.08977909271868256 1105 88-89 0.27144885184228684 1105 90-91 0.1704903705493379 1105 92-93 0.23264910158851748 1105 94-95 0.20379047003691753 1105 96-97 0.3144740621198103 1105 98-99 0.3568852469633512 1105 100-101 0.6611121900444594 1106 1 0.03689715860499376 1106 2 0.026070954061420792 1106 3 0.0666623981996679 1106 4 0.07073771092779424 1106 5 -0.05365678180142197 1106 6 -0.07348947591113131 1106 7 0.07547896244836494 1106 8 -0.029439417641668797 1106 9 0.0157467699565359 1106 10-11 -0.004453981157809039 1106 12-13 -0.09348075883455209 1106 14-15 -0.033271684998844364 1106 16-17 0.05169983471653694 1106 18-19 -0.01639532850990122 1106 20-21 0.04165011661957152 1106 22-23 -0.008042396512117023 1106 24-25 -0.19459220960577284 1106 26-27 -0.0700770493344649 1106 28-29 0.029913074063159684 1106 30-31 0.09014986867074981 1106 32-33 -0.10716072042023228 1106 34-35 -0.010494566933211047 1106 36-37 -0.15611880388727428 1106 38-39 -0.03946623137270677 1106 40-41 -0.0798046663415306 1106 42-43 -0.24295918871907674 1106 44-45 -0.21269083047349824 1106 46-47 -0.3404425429122213 1106 48-49 -0.3301117901287043 1106 50-51 -0.22028675735673175 1106 52-53 -0.29003970112696464 1106 54-55 -0.2969685536673339 1106 56-57 -0.11062228841078792 1106 58-59 -0.07325433654160918 1106 60-61 -0.33500215341900486 1106 62-63 -0.4287402724424396 1106 64-65 -0.41010932697758307 1106 66-67 -0.27937278165773094 1106 68-69 -0.17213782158945534 1106 70-71 -0.11780189194649893 1106 72-73 -0.39042303263427414 1106 74-75 -0.316149749242701 1106 76-77 -0.3537962034766373 1106 78-79 -0.2218997498565578 1106 80-81 -0.23939485268686767 1106 82-83 0.03432680161082047 1106 84-85 -0.1836756537192663 1106 86-87 0.005860244131927317 1106 88-89 0.02792812503126285 1106 90-91 0.2575924966107479 1106 92-93 0.29970239420030254 1106 94-95 0.4061878654591773 1106 96-97 0.16018328623703226 1106 98-99 0.3712567033687968 1106 100-101 0.7334046169039077 1107 1 0.03174052463893062 1107 2 0.06019697071486618 1107 3 0.07626941044788538 1107 4 0.06329783940827838 1107 5 -0.036729219770244015 1107 6 0.01611792332649742 1107 7 -0.03251365895344094 1107 8 -0.017903908137853364 1107 9 0.10616362205563235 1107 10-11 0.06807912256179804 1107 12-13 0.060123402591628405 1107 14-15 0.13101015939271576 1107 16-17 0.07822957162823485 1107 18-19 0.026086434613873166 1107 20-21 -0.03621860367093177 1107 22-23 -0.06391560587984912 1107 24-25 -0.0266839518966151 1107 26-27 -0.011288519700755728 1107 28-29 0.036395146778467335 1107 30-31 -0.09431508621273821 1107 32-33 0.06641209486326716 1107 34-35 0.13202879755277053 1107 36-37 0.3038326289410378 1107 38-39 0.2791824660854161 1107 40-41 0.2600768503989883 1107 42-43 0.03439935052487186 1107 44-45 0.4164862365337214 1107 46-47 0.2547713654873789 1107 48-49 0.22046450160971887 1107 50-51 0.30996775268241805 1107 52-53 0.22850999153894946 1107 54-55 0.3943574010678006 1107 56-57 0.29573756014735153 1107 58-59 0.37132877911018625 1107 60-61 0.43115787960409335 1107 62-63 0.26985624594279045 1107 64-65 0.3718479364912568 1107 66-67 0.3319753604705866 1107 68-69 0.4370528545455521 1107 70-71 0.31208046327108363 1107 72-73 0.2848863976528122 1107 74-75 0.1979334405820552 1107 76-77 0.11500599978310433 1107 78-79 0.31647670054752197 1107 80-81 0.4302290580991688 1107 82-83 0.39508317242408 1107 84-85 0.525364741525923 1107 86-87 0.6095250338999278 1107 88-89 0.5771386666229503 1107 90-91 0.5176526089666709 1107 92-93 0.44293685173789044 1107 94-95 0.7793520844177841 1107 96-97 0.9313854688573393 1107 98-99 1.2435421338622739 1107 100-101 1.5006224209969652 1108 1 -0.05345516092620883 1108 2 0.07129291032111595 1108 3 0.04977892064411549 1108 4 -0.07485987454389686 1108 5 -0.085323465611161 1108 6 -0.011211631765171148 1108 7 0.029229891235551975 1108 8 -0.01744955262622483 1108 9 0.009145217577838594 1108 10-11 -0.14859562995894748 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-0.9411999370760107 2316 64-65 -1.2220159541016038 2316 66-67 -2.2054168381365677 2316 68-69 -1.3440201076021765 2316 70-71 -0.9697547765080792 2316 72-73 -0.7546257446630698 2316 74-75 -0.9181492642856988 2316 76-77 -0.9745458350958351 2316 78-79 -1.0843911232174968 2316 80-81 -1.6642708156063115 2316 82-83 -1.2062095421863894 2316 84-85 -1.3124550542377982 2316 86-87 -1.7692502701090227 2316 88-89 -1.2812039884486772 2316 90-91 -1.3260579239961956 2316 92-93 -1.4507592475656885 2316 94-95 -1.6662059569346468 2316 96-97 -1.6809489951126402 2316 98-99 -1.982484460154975 2316 100-101 -2.1190145724718015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 16.0 5 50.0 6 121.0 7 272.0 8 641.0 9 1241.0 10 1975.0 11 3316.0 12 4896.0 13 6489.0 14 8172.0 15 11049.0 16 14029.0 17 18097.0 18 15829.0 19 9792.0 20 8349.0 21 8498.0 22 8962.0 23 9741.0 24 11396.0 25 13433.0 26 16717.0 27 20495.0 28 25401.0 29 31039.0 30 38093.0 31 47303.0 32 58427.0 33 75274.0 34 98009.0 35 132439.0 36 185901.0 37 235952.0 38 136921.0 39 7450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41097785066487 19.06771255260714 14.913516824405763 21.607792772322224 2 15.991663091561408 21.07336906068356 39.24446372236009 23.69050412539494 3 20.001990939644507 25.50354181842314 30.131133397696512 24.363333844235836 4 12.916500761583336 17.02454763213945 36.80485770083995 33.254093905437266 5 13.159048588897148 38.021130004116024 33.51656642568276 15.303254981304063 6 30.9606159621802 36.33061356052179 17.29028149964607 15.418488977651936 7 28.167243775246032 33.775269732096795 20.286492341164788 17.770994151492385 8 27.43300049850173 33.08218642384656 19.492444657222784 19.99236842042893 9 27.199146128346204 16.405269860732332 20.451837783346658 35.943746227574806 10-11 25.20848705863392 26.625274730483667 26.470351228406695 21.69588698247571 12-13 26.88457217616359 24.24418025909757 27.43636719867149 21.43488036606735 14-15 23.29395257560268 25.19707496353975 27.04054948718638 24.468422973671196 16-17 21.47208849706441 28.527417728249322 27.73579815122857 22.264695623457694 18-19 21.328171351871912 26.380024221969943 31.266407698573147 21.025396727585 20-21 22.331494960076412 25.097350040646454 31.351058190399222 21.220096808877916 22-23 21.87949452371726 25.18671345217738 31.412180586383144 21.52161143772222 24-25 21.926919485345817 25.516005125685147 31.5225291558894 21.034546233079627 26-27 21.68295950078235 25.453519106009487 31.58822839278667 21.27529300042149 28-29 21.16095059767881 25.976353566716444 31.613495769239886 21.249200066364864 30-31 21.418552169360073 25.554313893487517 31.444058251989983 21.583075685162427 32-33 21.240012624571857 25.699733485649816 31.37723571647803 21.683018173300294 34-35 22.02360447881475 25.46630617875439 31.123014129819893 21.387075212610966 36-37 22.097039450942805 25.32326006855709 31.067494008069303 21.512206472430798 38-39 21.683367715133812 25.62106489034826 31.24693348840039 21.448633906117536 40-41 21.766953290166 25.66208606289284 31.166592403687236 21.40436824325392 42-43 21.606402985658303 25.935022904767756 30.524350708987193 21.93422340058675 44-45 21.840041207923043 25.82921464777338 30.31930610155351 22.011438042750058 46-47 22.376368015939416 26.353203090868725 29.45762063781361 21.81280825537825 48-49 22.484957939111254 26.549276969846357 28.939218697660895 22.026546393381494 50-51 22.289686086963734 26.854379570549554 29.00680647237021 21.849127870116504 52-53 22.67600604225788 26.934304666316937 28.608724299862846 21.78096499156233 54-55 22.03630660933559 26.787491186038288 28.812026095252012 22.364176109374103 56-57 22.473887415476284 27.077413019279188 28.257288652194834 22.191410913049694 58-59 22.66082414200922 26.52694747969034 28.451536092060447 22.360692286239995 60-61 22.264231141315747 26.760546132627134 29.091718536994076 21.883504189063046 62-63 22.29178311906093 26.730885841429863 29.02369604591862 21.953634993590594 64-65 22.02827052508781 26.984678799160438 28.899641255640812 22.087409420110937 66-67 22.05446432380588 27.12226623666992 28.417905330137327 22.405364109386873 68-69 22.215730679727702 26.869060584262527 28.685612753550366 22.229595982459404 70-71 22.29139295727803 26.984473715603468 28.414174874327397 22.309958452791104 72-73 22.34287337238465 26.855737276228194 28.51081613926063 22.290573212126528 74-75 22.16871696275178 27.576663402363017 28.04300190051208 22.211617734373117 76-77 22.324493158433835 27.110804574817177 28.190594471049106 22.374107795699878 78-79 22.282011222617466 27.118520874028984 28.25148775723436 22.34798014611919 80-81 22.37787679836932 27.356426726079018 27.98136253387373 22.284333941677925 82-83 22.308005293408918 27.2656382705564 28.069209346422014 22.357147089612674 84-85 22.162014825583118 27.284603396887537 27.93514350556108 22.618238271968274 86-87 22.34059825058928 27.2692941404656 27.71464609056912 22.675461518376004 88-89 22.239240052272873 27.284370442122963 27.505834872707336 22.97055463289683 90-91 22.13230132144876 27.070914339510683 27.95506702849239 22.841717310548162 92-93 22.29103390140495 27.056696952495844 28.02764475634859 22.624624389750615 94-95 21.83092805333093 26.927680431552027 28.13565320989732 23.105738305219724 96-97 21.884808729527332 26.794377822879774 28.20801992142351 23.112793526169384 98-99 21.765351635194214 26.894223244727478 27.740923960081737 23.59950115999657 100-101 21.42503026915351 26.926767412416968 28.00259139506726 23.645610923362256 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 431.0 1 349.5 2 547.5 3 760.5 4 536.0 5 434.0 6 693.0 7 1307.0 8 2006.0 9 2432.0 10 2273.5 11 1888.0 12 1881.0 13 2049.0 14 2243.0 15 2456.5 16 2584.0 17 2699.0 18 2909.0 19 3147.5 20 3358.5 21 3705.5 22 4215.5 23 4821.5 24 5563.0 25 6603.0 26 8011.0 27 9781.5 28 11433.5 29 13447.5 30 16173.5 31 18753.5 32 21955.5 33 24129.0 34 25496.5 35 27213.0 36 29948.0 37 32767.0 38 33768.0 39 33872.5 40 34293.5 41 35966.5 42 37078.5 43 38385.0 44 40251.5 45 42160.5 46 42752.0 47 45334.5 48 54534.5 49 53959.5 50 45620.5 51 42102.5 52 39845.5 53 39855.5 54 39412.5 55 37165.5 56 34214.0 57 31109.5 58 29488.0 59 26890.0 60 22462.5 61 18913.0 62 16150.0 63 12452.0 64 8794.5 65 6686.0 66 5004.5 67 3942.0 68 2968.5 69 2005.0 70 1584.5 71 1256.0 72 1052.0 73 974.0 74 788.0 75 622.0 76 466.5 77 277.0 78 171.5 79 119.0 80 84.5 81 61.5 82 44.0 83 30.0 84 14.5 85 8.0 86 5.0 87 2.0 88 1.5 89 1.5 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040922962917317444 2 0.007584178455719063 3 0.004661109675910674 4 7.110167302236621E-4 5 7.900185891374025E-4 6 7.900185891374025E-5 7 0.0 8 0.0 9 0.002291053908498467 10-11 5.530130123961817E-4 12-13 0.0011850278837061035 14-15 0.0024490576263259474 16-17 0.002646562273610298 18-19 0.0 20-21 0.0 22-23 0.001303530672076714 24-25 8.690204480511426E-4 26-27 0.004147597592971362 28-29 0.004977117111565635 30-31 0.001303530672076714 32-33 5.925139418530517E-4 34-35 0.007979187750287765 36-37 0.010467746306070582 38-39 0.008216193327028986 40-41 0.001659039037188545 42-43 6.715158007667921E-4 44-45 0.0013430316015335842 46-47 0.0 48-49 7.110167302236621E-4 50-51 9.87523236421753E-4 52-53 0.00323907621546335 54-55 0.003594584580575181 56-57 0.0018960446139297657 58-59 0.001264029742619844 60-61 0.004463605028626323 62-63 0.005964640347987388 64-65 0.01015173887041562 66-67 0.003713087368945791 68-69 0.0034365808627477004 70-71 3.16007435654961E-4 72-73 0.0017775418255591554 74-75 0.006438651501469829 76-77 0.002962569709265259 78-79 0.003594584580575181 80-81 0.004977117111565635 82-83 0.005372126406134337 84-85 0.005964640347987388 86-87 0.004147597592971362 88-89 0.01003323608204501 90-91 0.013785824380447672 92-93 0.0015405362488179347 94-95 0.0028440669208946484 96-97 0.001580037178274805 98-99 0.004463605028626323 100-101 0.005174621758849986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1265793.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.635796296467575 #Duplication Level Percentage of deduplicated Percentage of total 1 80.27523449731711 46.26727063138536 2 11.795025616326928 13.596313874684714 3 3.3437073971450437 5.781517152505308 4 1.4522788203509154 3.348129850216784 5 0.7478208070942296 2.1550623851971493 6 0.46856308592114126 1.6203603943317082 7 0.32993085259765903 1.3311079188566985 8 0.24422391278990604 1.126083175062823 9 0.17819121336617016 0.9243173227853677 >10 1.0293957057111855 11.169225875122386 >50 0.08439939035214696 3.313796556166422 >100 0.04558253920358308 4.900847729590209 >500 0.0037182041280668173 1.4574893718056119 >1k 0.0016525351680296966 1.8022769245542685 >5k 2.7542252800494943E-4 1.2062008377351132 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8044 0.6354909531021264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7153 0.565100296809984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4550 0.3594584580575181 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3728 0.2945189300304236 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2159 0.17056501339476518 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1826 0.14425739437648966 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1476 0.11660674375668059 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1441 0.11384167869469969 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1350 0.10665250953354932 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 0.10333443145917223 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1306 0.10317642774134476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1293 0.10214940357546613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 7.900185891374025E-5 5 0.0 0.0 0.0 0.0 7.900185891374025E-5 6 0.0 0.0 0.0 0.0 7.900185891374025E-5 7 0.0 0.0 0.0 0.0 7.900185891374025E-5 8 0.0 0.0 0.0 0.0 7.900185891374025E-5 9 0.0 0.0 0.0 0.0 7.900185891374025E-5 10-11 0.0 0.0 0.0 0.0 7.900185891374025E-5 12-13 0.0 0.0 0.0 0.0 7.900185891374025E-5 14-15 0.0 0.0 0.0 0.0 7.900185891374025E-5 16-17 0.0 0.0 0.0 0.0 7.900185891374025E-5 18-19 0.0 0.0 0.0 0.0 7.900185891374025E-5 20-21 0.0 0.0 0.0 0.0 1.9750464728435062E-4 22-23 0.0 0.0 0.0 7.900185891374025E-5 2.7650650619809086E-4 24-25 0.0 0.0 0.0 3.16007435654961E-4 3.16007435654961E-4 26-27 0.0 0.0 0.0 5.530130123961817E-4 3.16007435654961E-4 28-29 0.0 0.0 0.0 0.0018565436844728957 3.16007435654961E-4 30-31 0.0 0.0 0.0 0.0030810724976358694 3.16007435654961E-4 32-33 0.0 0.0 0.0 0.005451128265048077 3.16007435654961E-4 34-35 0.0 0.0 0.0 0.007742182173546544 3.16007435654961E-4 36-37 0.0 0.0 0.0 0.011573772330862944 3.16007435654961E-4 38-39 0.0 0.0 0.0 0.0159978764300324 3.950092945687012E-4 40-41 0.0 0.0 0.0 0.021014494471054905 3.950092945687012E-4 42-43 0.0 0.0 0.0 0.029783700810480068 4.345102240255713E-4 44-45 1.580037178274805E-4 0.0 0.0 0.037012370901087305 4.740111534824414E-4 46-47 1.9750464728435062E-4 0.0 0.0 0.05135120829393115 4.740111534824414E-4 48-49 3.16007435654961E-4 0.0 0.0 0.06920562840843646 5.135120829393116E-4 50-51 3.16007435654961E-4 0.0 0.0 0.07757982545329292 5.530130123961817E-4 52-53 3.16007435654961E-4 0.0 0.0 0.08860058477175968 5.925139418530518E-4 54-55 3.16007435654961E-4 0.0 0.0 0.1074820290521436 6.32014871309922E-4 56-57 3.16007435654961E-4 0.0 0.0 0.1425193534803874 6.32014871309922E-4 58-59 3.16007435654961E-4 0.0 0.0 0.18494335171706588 6.32014871309922E-4 60-61 3.16007435654961E-4 7.900185891374025E-5 0.0 0.23396400517304172 6.32014871309922E-4 62-63 3.16007435654961E-4 7.900185891374025E-5 0.0 0.28606573112665346 6.32014871309922E-4 64-65 3.16007435654961E-4 7.900185891374025E-5 0.0 0.3442506002166231 7.110167302236622E-4 66-67 3.16007435654961E-4 7.900185891374025E-5 0.0 0.4218699265993729 7.110167302236622E-4 68-69 3.16007435654961E-4 7.900185891374025E-5 0.0 0.5252833599174589 7.110167302236622E-4 70-71 3.555083651118311E-4 7.900185891374025E-5 0.0 0.5816511862524125 7.110167302236622E-4 72-73 4.345102240255713E-4 7.900185891374025E-5 0.0 0.6517258351089001 8.295195185942725E-4 74-75 5.530130123961817E-4 7.900185891374025E-5 0.0 0.7280416308195732 8.690204480511426E-4 76-77 5.530130123961817E-4 7.900185891374025E-5 0.0 0.8106380743138886 8.690204480511426E-4 78-79 5.530130123961817E-4 7.900185891374025E-5 0.0 0.8968291023887792 8.690204480511426E-4 80-81 6.32014871309922E-4 7.900185891374025E-5 0.0 1.0360303777947895 8.690204480511426E-4 82-83 6.32014871309922E-4 7.900185891374025E-5 0.0 1.186805425531663 8.690204480511426E-4 84-85 6.32014871309922E-4 7.900185891374025E-5 0.0 1.4141332745559503 8.690204480511426E-4 86-87 6.32014871309922E-4 7.900185891374025E-5 0.0 1.742109491836343 8.690204480511426E-4 88-89 6.32014871309922E-4 7.900185891374025E-5 0.0 2.0908236970815923 8.690204480511426E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1220 0.0 33.093285 2 GTACCTG 1400 0.0 30.889954 1 GTACATG 7365 0.0 25.358507 1 GTACACG 300 0.0 25.345602 1 GTATCAA 13935 0.0 23.974648 1 TAACGCA 120 1.7117505E-4 23.748362 4 TACATGG 7705 0.0 23.67224 2 GGTATCA 10430 0.0 22.827446 1 ACATGGG 7490 0.0 22.57521 3 GAGTACT 8275 0.0 22.301268 12-13 TAAGGTG 625 0.0 22.038479 5 AGTACTT 8445 0.0 21.65547 12-13 GTACTTT 8935 0.0 20.49405 14-15 GTAAGGT 585 0.0 20.297745 4 TCAACGC 16420 0.0 20.248297 4 ATCAACG 16430 0.0 20.235973 3 CAACGCA 16535 0.0 20.164923 5 TATCAAC 16710 0.0 20.01138 2 AACGCAG 16820 0.0 19.880507 6 CATGGGG 2860 0.0 19.430477 4 >>END_MODULE